Restriction map for
pFN21AE9536
(Circular) (Six-base) MAPSORT of: pFN21AE9536.seq Check: 3152 from: 1 to: 8350
pFN21AE9536 8350 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4763 4763
Size: 8350
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6160 278
Size: 53 83 186 936 4624 2468
Fragments arranged by size:
4624 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3318 5693 1937
Size: 1381 2375 4594
Fragments arranged by size:
4594 2375 1381
Acc65I G'GTAC_C
Cuts at: 2472 5671 2472
Size: 3199 5151
AclI AA'CG_TT
Cuts at: 1563 6477 6850 1563
Size: 4914 373 3063
Fragments arranged by size:
4914 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2306 2521 2918 3902 4374 5049 5232 6413
Size: 674 215 397 984 472 675 183 1181
Cuts at: 6413 7797 8065 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1181 984 675 674 472 397
268 215 183
AfeI AGC'GCT
Cuts at: 4648 4648
Size: 8350
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8153
AflIII A'CryG_T
Cuts at: 1203 7249 8226 1203
Size: 6046 977 1327
Fragments arranged by size:
6046 1327 977
AgeI A'CCGG_T
Cuts at: 7181 7181
Size: 8350
AhdI GACnn_n'nnGTC
Cuts at: 5052 7079 5052
Size: 2027 6323
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8350
AlwNI CAG_nnn'CTG
Cuts at: 2482 3758 6093 7665 2482
Size: 1276 2335 1572 3167
Fragments arranged by size:
3167 2335 1572 1276
ApaI G_GGCC'C
Cuts at: 2733 2733
Size: 8350
ApaLI G'TGCA_C
Cuts at: 6407 7563 6407
Size: 1156 7194
ApoI r'AATT_y
Cuts at: 1493 2900 3555 3655 3761 4540 4843 5659 5885
Size: 1407 655 100 106 779 303 816 226
Cuts at: 5885 6037 8146 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1407 816 779 655 303 226
152 106 100
AseI AT'TA_AT
Cuts at: 160 1032 6904 160
Size: 872 5872 1606
Fragments arranged by size:
5872 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8350
AvaI C'yCGr_G
Cuts at: 1870 1943 2109 2520 2733 2840 5675 1870
Size: 73 166 411 213 107 2835 4545
Fragments arranged by size:
4545 2835 411 213 166 107 73
AvrII C'CTAG_G
Cuts at: 3031 4252 5615 3031
Size: 1221 1363 5766
Fragments arranged by size:
5766 1363 1221
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4084 4117 4084
Size: 33 8317
BamHI G'GATC_C
Cuts at: 4082 4132 5680 6047 4082
Size: 50 1548 367 6385
Fragments arranged by size:
6385 1548 367 50
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2204 2236 2472 4331
Size: 325 217 627 285 132 32 236 1859
Cuts at: 4331 4621 4680 4982 5671 7126 618
Size: 290 59 302 689 1455 1842
Fragments arranged by size:
1859 1842 1455 689 627 325 302 290
285 236 217 132 59 32
BanII G_rGCy'C
Cuts at: 1391 2511 2733 2772 3466 3834 3867 5669 1391
Size: 1120 222 39 694 368 33 1802 4072
Fragments arranged by size:
4072 1802 1120 694 368 222 39 33
BbeI G_GCGC'C
Cuts at: 2076 4335 2076
Size: 2259 6091
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2566 3461 4380 4429 4599 4745 928
Size: 971 667 895 919 49 170 146 4533
Fragments arranged by size:
4533 971 919 895 667 170 146 49
BbvCI CC'TCA_GC
Cuts at: 4658 5169 4658
Size: 511 7839
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2135 2169 2280 2314 6541 6575 1903
Size: 34 198 34 111 34 4227 34 3678
Fragments arranged by size:
4227 3678 198 111 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6242 7452 6242
Size: 1210 7140
BclI T'GATC_A
Cuts at: 1548 4203 1548
Size: 2655 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2845 6961 136
Size: 107 122 71 631 605 1173 4116 1525
Fragments arranged by size:
4116 1525 1173 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5628 8345 5628
Size: 2717 5633
BlpI GC'TnA_GC
Cuts at: 2049 5767 2049
Size: 3718 4632
Bme1580I G_kGCm'C
Cuts at: 1790 2241 2733 4683 6411 7567 1790
Size: 451 492 1950 1728 1156 2573
Fragments arranged by size:
2573 1950 1728 1156 492 451
BmgBI CAC'GTC
Cuts at: 1318 2248 1318
Size: 930 7420
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4686 6081 7039
Size: 416 53 83 399 24 3269 1395 958
Cuts at: 7039 442
Size: 1753
Fragments arranged by size:
3269 1753 1395 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2206 2519 2564 2678 2834 3108 4135 4208
Size: 967 313 45 114 156 274 1027 73
Cuts at: 4208 5265 7010 1239
Size: 1057 1745 2579
Fragments arranged by size:
2579 1745 1057 1027 967 313 274 156
114 73 45
Bpu10I CC'TnA_GC
Cuts at: 4658 5169 4658
Size: 511 7839
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2944 3895 4430 5458 6475 7340 7638 2944
Size: 951 535 1028 1017 865 298 3656
Fragments arranged by size:
3656 1028 1017 951 865 535 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2654 2805 4412 4473 5528 7013 882
Size: 617 1155 151 1607 61 1055 1485 2219
Fragments arranged by size:
2219 1607 1485 1155 1055 617 151 61
BsaAI yAC'GTr
Cuts at: 493 1204 4474 5089 6141 493
Size: 711 3270 615 1052 2702
Fragments arranged by size:
3270 2702 1052 711 615
BsaBI GATnn'nnATC
Cuts at: 4081 8344 4081
Size: 4263 4087
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 4332 6157 6539
Size: 53 83 186 936 540 2259 1825 382
Cuts at: 6539 7174 275
Size: 635 1451
Fragments arranged by size:
2259 1825 1451 936 635 540 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3124 6783 7181 7455 7602 8013 3124
Size: 3659 398 274 147 411 3461
Fragments arranged by size:
3659 3461 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2609 2639 2651 2681 3106
Size: 30 165 30 1206 30 12 30 425
Cuts at: 3106 3136 1178
Size: 30 6392
Fragments arranged by size:
6392 1206 425 165 30 30 30 30
30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2021 2164 2246 2609 2617 4430 4538 4541
Size: 835 143 82 363 8 1813 108 3
Cuts at: 4541 4913 5321 5462 1186
Size: 372 408 141 4074
Fragments arranged by size:
4074 1813 835 408 372 363 143 141
108 82 8 3
BseYI C'CCAG_C
Cuts at: 2053 2142 2510 3735 4754 5345 5539 7553 8049
Size: 89 368 1225 1019 591 194 2014 496
Cuts at: 8049 2053
Size: 2354
Fragments arranged by size:
2354 2014 1225 1019 591 496 368 194
89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5349 1163
Size: 573 3613 4164
Fragments arranged by size:
4164 3613 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2099 2308 5800 6561 7589 1540
Size: 275 186 98 209 3492 761 1028 2301
Fragments arranged by size:
3492 2301 1028 761 275 209 186 98
BsiHKAI G_wGCw'C
Cuts at: 3867 4125 4241 4313 6411 6496 7567 3867
Size: 258 116 72 2098 85 1071 4650
Fragments arranged by size:
4650 2098 1071 258 116 85 72
BsmI GAATG_Cn'
Cuts at: 3821 5861 5954 3821
Size: 2040 93 6217
Fragments arranged by size:
6217 2040 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4264 4264
Size: 8350
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 2511 2733 2772 3466 3834 3867
Size: 399 451 270 222 39 694 368 33
Cuts at: 3867 4125 4241 4313 4683 5669 6411 6496 7567
Size: 258 116 72 370 986 742 85 1071
Cuts at: 7567 1391
Size: 2174
Fragments arranged by size:
2174 1071 986 742 694 451 399 370
368 270 258 222 116 85 72 39
33
BspEI T'CCGG_A
Cuts at: 3124 8013 3124
Size: 4889 3461
BspHI T'CATG_A
Cuts at: 2913 3582 4266 6239 7969 2913
Size: 669 684 1973 1730 3294
Fragments arranged by size:
3294 1973 1730 684 669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4763 5584 5705 844
Size: 3919 821 121 3489
Fragments arranged by size:
3919 3489 821 121
BsrBI CCG'CTC
Cuts at: 1394 1823 2193 6237 1394
Size: 429 370 4044 3507
Fragments arranged by size:
4044 3507 429 370
BsrDI GCAATG_nn'
Cuts at: 65 1248 6845 7019 65
Size: 1183 5597 174 1396
Fragments arranged by size:
5597 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2560 4302 6994 7181 1700
Size: 110 750 1742 2692 187 2869
Fragments arranged by size:
2869 2692 1742 750 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8350
BssHII G'CGCG_C
Cuts at: 1925 2355 1925
Size: 430 7920
BssSI C'ACGA_G
Cuts at: 4026 5325 5505 6410 7422 4026
Size: 1299 180 905 1012 4954
Fragments arranged by size:
4954 1299 1012 905 180
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2452 794
Size: 1658 6692
BstBI TT'CG_AA
Cuts at: 7154 7154
Size: 8350
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8350
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2248
Size: 8350
BstYI r'GATC_y
Cuts at: 1972 3386 4082 4132 4284 4867 5628 5680 6047
Size: 1414 696 50 152 583 761 52 367
Cuts at: 6047 6432 6449 7901 7987 7999 8345 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1414 761 696 583 385 367
346 152 86 52 50 17 12
Bsu36I CC'TnA_GG
Cuts at: 5160 5160
Size: 8350
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 2241 2674 2713 4339 4611
Size: 552 939 9 228 433 39 1626 272
Cuts at: 4611 513
Size: 4252
Fragments arranged by size:
4252 1626 939 552 433 272 228 39
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7611
Fragments arranged by size:
7611 683 56
BtsI GCAGTG_nn'
Cuts at: 3807 4313 4356 4956 5866 6660 6680 3807
Size: 506 43 600 910 794 20 5477
Fragments arranged by size:
5477 910 794 600 506 43 20
ClaI AT'CG_AT
Cuts at: 1554 4694 1554
Size: 3140 5210
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8315
DraI TTT'AAA
Cuts at: 5644 5654 5644
Size: 10 8340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7357 809
Size: 507 6041 1802
Fragments arranged by size:
6041 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2096 2305 2339
Size: 54 1425 50 275 18 266 209 34
Cuts at: 2339 2349 2526 2643 3682 5797 6686 8315 8
Size: 10 177 117 1039 2115 889 1629 43
Fragments arranged by size:
2115 1629 1425 1039 889 275 266 209
177 117 54 50 43 34 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2096 2305 5797 1537
Size: 275 284 209 3492 4090
Fragments arranged by size:
4090 3492 284 275 209
EarI CTCTTCn'nnn_
Cuts at: 1353 4485 5587 5619 6280 1353
Size: 3132 1102 32 661 3423
Fragments arranged by size:
3423 3132 1102 661 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2793 3588 6937 7311 7457 387
Size: 1064 138 1204 795 3349 374 146 1280
Fragments arranged by size:
3349 1280 1204 1064 795 374 146 138
EcoICRI GAG'CTC
Cuts at: 3865 3865
Size: 8350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2206 2306 2519 2521 2564 2678 2834
Size: 393 574 100 213 2 43 114 156
Cuts at: 2834 2918 3108 3902 4135 4208 4374 5049 5232
Size: 84 190 794 233 73 166 675 183
Cuts at: 5232 5265 6413 7010 7797 8065 1239
Size: 33 1148 597 787 268 1524
Fragments arranged by size:
1524 1148 794 787 675 597 574 393
268 233 213 190 183 166 156 114
100 84 73 43 33 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2498 2498
Size: 8350
EcoO109I rG'GnC_Cy
Cuts at: 2118 2697 2729 2730 2737 4249 5612 2118
Size: 579 32 1 7 1512 1363 4856
Fragments arranged by size:
4856 1512 1363 579 32 7 1
EcoRI G'AATT_C
Cuts at: 2900 3555 5659 6037 2900
Size: 655 2104 378 5213
Fragments arranged by size:
5213 2104 655 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2806 5020 5237 5586 2806
Size: 2214 217 349 5570
Fragments arranged by size:
5570 2214 349 217
FspI TGC'GCA
Cuts at: 6856 6856
Size: 8350
HaeII r_GCGC'y
Cuts at: 1678 2076 2381 2483 4335 4650 4731 7497 1678
Size: 398 305 102 1852 315 81 2766 2531
Fragments arranged by size:
2766 2531 1852 398 315 305 102 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2237 2269 2537 2569 2609
Size: 32 135 32 665 32 268 32 40
Cuts at: 2609 2641 2942 2974 3106 3138 3246 3278 3378
Size: 32 301 32 132 32 108 32 100
Cuts at: 3378 3410 4592 4624 4852 4884 5038 5070 5527
Size: 32 1182 32 228 32 154 32 457
Cuts at: 5527 5559 6991 7023 7065 7097 1373
Size: 32 1432 32 42 32 2626
Fragments arranged by size:
2626 1432 1182 665 457 301 268 228
154 135 132 108 100 42 40 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2923 4948 5694 5940 669
Size: 988 281 985 2025 746 246 3079
Fragments arranged by size:
3079 2025 988 985 746 281 246
HindIII A'AGCT_T
Cuts at: 748 5261 748
Size: 4513 3837
HpaI GTT'AAC
Cuts at: 5940 5940
Size: 8350
KasI G'GCGC_C
Cuts at: 2072 4331 2072
Size: 2259 6091
KpnI G_GTAC'C
Cuts at: 2476 5675 2476
Size: 3199 5151
MfeI C'AATT_G
Cuts at: 5949 5949
Size: 8350
MluI A'CGCG_T
Cuts at: 8226 8226
Size: 8350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3348 7464 7648 1305
Size: 2043 4116 184 2007
Fragments arranged by size:
4116 2043 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2341 2351 2645 3684 10
Size: 54 1425 343 509 10 294 1039 4676
Fragments arranged by size:
4676 1425 1039 509 343 294 54 10
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 3311 3587 3646 4991 6308 6667
Size: 648 1080 1065 276 59 1345 1317 359
Cuts at: 6667 6826 518
Size: 159 2042
Fragments arranged by size:
2042 1345 1317 1080 1065 648 359 276
159 59
MspA1I CmG'CkG
Cuts at: 1606 2053 2284 2715 2751 3735 5168 5766 6443
Size: 447 231 431 36 984 1433 598 677
Cuts at: 6443 7591 7836 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1433 1148 984 677 598 447 431
245 231 36
NaeI GCC'GGC
Cuts at: 4304 4304
Size: 8350
NarI GG'CG_CC
Cuts at: 2073 4332 2073
Size: 2259 6091
NcoI C'CATG_G
Cuts at: 513 1065 2004 2013 2241 4339 513
Size: 552 939 9 228 2098 4524
Fragments arranged by size:
4524 2098 939 552 228 9
NdeI CA'TA_TG
Cuts at: 387 4051 387
Size: 3664 4686
NgoMIV G'CCGG_C
Cuts at: 4302 4302
Size: 8350
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8350
NotI GC'GGCC_GC
Cuts at: 5797 5797
Size: 8350
NspI r_CATG'y
Cuts at: 1132 1229 3310 5708 7253 1132
Size: 97 2081 2398 1545 2229
Fragments arranged by size:
2398 2229 2081 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8350
PciI A'CATG_T
Cuts at: 7249 7249
Size: 8350
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2482 3658 5555 1108
Size: 1374 1176 1897 3903
Fragments arranged by size:
3903 1897 1374 1176
PfoI T'CCnGG_A
Cuts at: 2895 3085 3234 2895
Size: 190 149 8011
Fragments arranged by size:
8011 190 149
PmeI GTTT'AAAC
Cuts at: 5654 5654
Size: 8350
PmlI CAC'GTG
Cuts at: 4474 4474
Size: 8350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3487 3519 6419 6451 7932 7964 3487
Size: 32 2900 32 1481 32 3873
Fragments arranged by size:
3873 2900 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2118 2118
Size: 8350
PshAI GACnn'nnGTC
Cuts at: 1613 3919 1613
Size: 2306 6044
PsiI TTA'TAA
Cuts at: 5920 5920
Size: 8350
PspOMI G'GGCC_C
Cuts at: 2729 2729
Size: 8350
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2816 2848 2816
Size: 32 8318
PstI C_TGCA'G
Cuts at: 830 2427 5702 830
Size: 1597 3275 3478
Fragments arranged by size:
3478 3275 1597
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8350
PvuII CAG'CTG
Cuts at: 2053 2284 2751 5168 2053
Size: 231 467 2417 5235
Fragments arranged by size:
5235 2417 467 231
SacI G_AGCT'C
Cuts at: 3867 3867
Size: 8350
SacII CC_GC'GG
Cuts at: 2716 2716
Size: 8350
SalI G'TCGA_C
Cuts at: 1936 5692 1936
Size: 3756 4594
SbfI CC_TGCA'GG
Cuts at: 5702 5702
Size: 8350
ScaI AGT'ACT
Cuts at: 6598 8310 6598
Size: 1712 6638
SexAI A'CCwGG_T
Cuts at: 2889 2889
Size: 8350
SfcI C'TryA_G
Cuts at: 826 1045 2423 4041 5376 5698 6833 7514 7705
Size: 219 1378 1618 1335 322 1135 681 191
Cuts at: 7705 826
Size: 1471
Fragments arranged by size:
1618 1471 1378 1335 1135 681 322 219
191
SfoI GGC'GCC
Cuts at: 2074 4333 2074
Size: 2259 6091
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8350
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8350
SmaI CCC'GGG
Cuts at: 1872 2522 2735 2842 5677 1872
Size: 650 213 107 2835 4545
Fragments arranged by size:
4545 2835 650 213 107
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2109 2959 3910 4445 5473 6454
Size: 197 926 166 850 951 535 1028 981
Cuts at: 6454 7355 7617 820
Size: 901 262 1553
Fragments arranged by size:
1553 1028 981 951 926 901 850 535
262 197 166
SnaBI TAC'GTA
Cuts at: 493 6141 493
Size: 5648 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8350
SphI G_CATG'C
Cuts at: 1132 3310 5708 1132
Size: 2178 2398 3774
Fragments arranged by size:
3774 2398 2178
SspI AAT'ATT
Cuts at: 5 52 6274 5
Size: 47 6222 2081
Fragments arranged by size:
6222 2081 47
StuI AGG'CCT
Cuts at: 1517 3030 3279 1517
Size: 1513 249 6588
Fragments arranged by size:
6588 1513 249
StyI C'CwwG_G
Cuts at: 513 1065 2004 2013 2089 2241 3031 3274 4252
Size: 552 939 9 76 152 790 243 978
Cuts at: 4252 4339 4410 4731 5027 5233 5522 5615 5789
Size: 87 71 321 296 206 289 93 174
Cuts at: 5789 513
Size: 3074
Fragments arranged by size:
3074 978 939 790 552 321 296 289
243 206 174 152 93 87 76 71
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2678 4698 5003 6377 6394 6547 6732
Size: 1255 1098 2020 305 1374 17 153 185
Cuts at: 6732 7177 325
Size: 445 1498
Fragments arranged by size:
2020 1498 1374 1255 1098 445 305 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3146 3420 6596
Size: 274 80 33 51 1443 1169 274 3176
Cuts at: 6596 8308 96
Size: 1712 138
Fragments arranged by size:
3176 1712 1443 1169 274 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2559 2591 2651 2683 8310 8342 2559
Size: 32 60 32 5627 32 2567
Fragments arranged by size:
5627 2567 60 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8350
XbaI T'CTAG_A
Cuts at: 5686 5686
Size: 8350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2653 2011
Size: 642 7708
XhoI C'TCGA_G
Cuts at: 1943 2109 1943
Size: 166 8184
XmaI C'CCGG_G
Cuts at: 1870 2520 2733 2840 5675 1870
Size: 650 213 107 2835 4545
Fragments arranged by size:
4545 2835 650 213 107
XmnI GAAnn'nnTTC
Cuts at: 6479 6479
Size: 8350
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6158 276
Size: 53 83 186 936 4624 2468
Fragments arranged by size:
4624 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BfrBI BplI BsiWI BstZ17I DraIII EcoRV
FseI FspAI NruI NsiI PacI PspXI RsrII SanDI
SapI SfiI SrfI SwaI