Restriction map for
pFN21AE9534
(Circular) (Six-base) MAPSORT of: pFN21AE9534.seq Check: 1127 from: 1 to: 5710
pFN21AE9534 5710 bp
With 182 enzymes: SgfI *
March 28, 2012 12:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2450 3773 5157 5425 1632
Size: 818 1323 1384 268 1917
Fragments arranged by size:
1917 1384 1323 818 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 2457 2808 4609 5586 1203
Size: 1254 351 1801 977 1327
Fragments arranged by size:
1801 1327 1254 977 351
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AlwNI CAG_nnn'CTG
Cuts at: 2721 3453 5025 2721
Size: 732 1572 3406
Fragments arranged by size:
3406 1572 732
ApaI G_GGCC'C
Cuts at: 2228 2228
Size: 5710
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 3019 3245 3397 5506 1493
Size: 1526 226 152 2109 1697
Fragments arranged by size:
2109 1697 1526 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 3035 1870
Size: 73 146 946 4545
Fragments arranged by size:
4545 946 146 73
BamHI G'GATC_C
Cuts at: 3040 3407 3040
Size: 367 5343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2145 2822 3031 4486
Size: 325 217 627 248 110 677 209 1455
Cuts at: 4486 618
Size: 1842
Fragments arranged by size:
1842 1455 677 627 325 248 217 209
110
BanII G_rGCy'C
Cuts at: 1391 2228 2924 3029 1391
Size: 837 696 105 4072
Fragments arranged by size:
4072 837 696 105
BbeI G_GCGC'C
Cuts at: 2149 2149
Size: 5710
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4739
BbvCI CC'TCA_GC
Cuts at: 2321 2321
Size: 5710
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2821 2855 3901 3935 1903
Size: 34 50 34 800 34 1046 34 3678
Fragments arranged by size:
3678 1046 800 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2928 4321 136
Size: 107 122 71 631 605 1256 1393 1525
Fragments arranged by size:
1525 1393 1256 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2228 3771 4927 1790
Size: 353 85 1543 1156 2573
Fragments arranged by size:
2573 1543 1156 353 85
BmgBI CAC'GTC
Cuts at: 1318 2458 1318
Size: 1140 4570
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 4399 442
Size: 416 53 83 399 24 2024 958 1753
Fragments arranged by size:
2024 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2507 2743 4370 1239
Size: 1268 236 1627 2579
Fragments arranged by size:
2579 1627 1268 236
Bpu10I CC'TnA_GC
Cuts at: 2321 2321
Size: 5710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4373 882
Size: 617 2874 2219
Fragments arranged by size:
2874 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2146 2448 2774 3517
Size: 53 83 186 936 613 302 326 743
Cuts at: 3517 3899 4534 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 743 635 613 382 326 302
186 83 53
BsaWI w'CCGG_w
Cuts at: 2580 4143 4541 4815 4962 5373 2580
Size: 1563 398 274 147 411 2917
Fragments arranged by size:
2917 1563 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 1178
Size: 30 165 30 861 30 4594
Fragments arranged by size:
4594 861 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2653 1186
Size: 1467 4243
BseYI C'CCAG_C
Cuts at: 2388 4913 5409 2388
Size: 2525 496 2689
Fragments arranged by size:
2689 2525 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2789 1163
Size: 573 1053 4084
Fragments arranged by size:
4084 1053 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2187 2766 3160 3921 4949 1540
Size: 275 186 186 579 394 761 1028 2301
Fragments arranged by size:
2301 1028 761 579 394 275 186 186
BsiHKAI G_wGCw'C
Cuts at: 2646 2691 2729 3771 3856 4927 2646
Size: 45 38 1042 85 1071 3429
Fragments arranged by size:
3429 1071 1042 85 45 38
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2228 2646 2691 2729 2924 3029
Size: 399 353 85 418 45 38 195 105
Cuts at: 3029 3771 3856 4927 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 418 399 353 195 105
85 85 45 38
BspEI T'CCGG_A
Cuts at: 2580 5373 2580
Size: 2793 2917
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 2184 3065 844
Size: 1292 48 881 3489
Fragments arranged by size:
3489 1292 881 48
BsrBI CCG'CTC
Cuts at: 1394 1823 2698 3597 1394
Size: 429 875 899 3507
Fragments arranged by size:
3507 899 875 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2301 4205 4379 65
Size: 1183 1053 1904 174 1396
Fragments arranged by size:
1904 1396 1183 1053 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2622 2776 4354 4541 1700
Size: 110 812 154 1578 187 2869
Fragments arranged by size:
2869 1578 812 187 154 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 2047 2253 2339 1925
Size: 122 206 86 5296
Fragments arranged by size:
5296 206 122 86
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2650 794
Size: 1856 3854
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 2032
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 3040 3407 3792 3809 5261 5347 5359 5705
Size: 1068 367 385 17 1452 86 12 346
Cuts at: 5705 1972
Size: 1977
Fragments arranged by size:
1977 1452 1068 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2714 2714
Size: 5710
BtgI C'CryG_G
Cuts at: 513 1065 2052 2295 2667 513
Size: 552 987 243 372 3556
Fragments arranged by size:
3556 987 552 372 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2169 2184
Size: 54 1425 50 275 18 273 66 15
Cuts at: 2184 2298 2763 3157 4046 5675 8
Size: 114 465 394 889 1629 43
Fragments arranged by size:
1629 1425 889 465 394 275 273 114
66 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2184 2763 3157 1537
Size: 275 372 579 394 4090
Fragments arranged by size:
4090 579 394 372 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2162 2879 2905 3640 1353
Size: 809 717 26 735 3423
Fragments arranged by size:
3423 809 735 717 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2687 4297 4671 4817 387
Size: 1064 138 1098 1610 374 146 1280
Fragments arranged by size:
1610 1280 1098 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 2507 2743 3773 4370 5157 5425
Size: 393 818 57 236 1030 597 787 268
Cuts at: 5425 1239
Size: 1524
Fragments arranged by size:
1524 1030 818 787 597 393 268 236
57
EcoNI CCTnn'n_nnAGG
Cuts at: 2636 2636
Size: 5710
EcoO109I rG'GnC_Cy
Cuts at: 2395 2985 2395
Size: 590 5120
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FspI TGC'GCA
Cuts at: 2071 2132 4216 2071
Size: 61 2084 3565
Fragments arranged by size:
3565 2084 61
FspAI rTGC'GCAy
Cuts at: 2071 2071
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2149 4857 1678
Size: 471 2708 2531
Fragments arranged by size:
2708 2531 471
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 4351 4383 4425
Size: 32 135 32 593 32 2154 32 42
Cuts at: 4425 4457 1373
Size: 32 2626
Fragments arranged by size:
2626 2154 593 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KasI G'GCGC_C
Cuts at: 2145 2145
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2022 4824 5008 1305
Size: 717 2802 184 2007
Fragments arranged by size:
2802 2007 717 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2105 2300 10
Size: 54 1425 343 273 195 3420
Fragments arranged by size:
3420 1425 343 273 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2411 3668 4027 4186 518
Size: 648 1245 1257 359 159 2042
Fragments arranged by size:
2042 1257 1245 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2388 3126 3803 4951 5196 1606
Size: 782 738 677 1148 245 2120
Fragments arranged by size:
2120 1148 782 738 677 245
NarI GG'CG_CC
Cuts at: 2146 2146
Size: 5710
NcoI C'CATG_G
Cuts at: 513 1065 2667 513
Size: 552 1602 3556
Fragments arranged by size:
3556 1602 552
NdeI CA'TA_TG
Cuts at: 387 2548 387
Size: 2161 3549
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 3068 4613 1132
Size: 97 1839 1545 2229
Fragments arranged by size:
2229 1839 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2990 1348
Size: 1642 4068
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5710
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2395 2985 2395
Size: 590 5120
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PspOMI G'GGCC_C
Cuts at: 2224 2224
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2214 2250 3062 830
Size: 1384 36 812 3478
Fragments arranged by size:
3478 1384 812 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2388 2388
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SanDI GG'GwC_CC
Cuts at: 2395 2985 2395
Size: 590 5120
SapI GCTCTTCn'nnn_
Cuts at: 2162 2879 2905 2162
Size: 717 26 4967
Fragments arranged by size:
4967 717 26
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2210 2246 3058 4193 4874 5065 826
Size: 219 1165 36 812 1135 681 191 1471
Fragments arranged by size:
1471 1165 1135 812 681 219 191 36
SfoI GGC'GCC
Cuts at: 2147 2147
Size: 5710
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 2776 1700
Size: 1076 4634
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3814 4715 4977 820
Size: 197 926 1871 901 262 1553
Fragments arranged by size:
1871 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2493 2667 2937 2980 3149 513
Size: 552 1428 174 270 43 169 3074
Fragments arranged by size:
3074 1428 552 270 174 169 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3737 3754 3907 4092 4537 325
Size: 1255 2157 17 153 185 445 1498
Fragments arranged by size:
2157 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3956 5668 96
Size: 274 80 33 51 1443 1979 1712 138
Fragments arranged by size:
1979 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 2501 1316
Size: 1185 4525
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI
XcmI