Restriction map for
pFN21AE9531
(Circular) (Six-base) MAPSORT of: pFN21AE9531.seq Check: 918 from: 1 to: 5590
pFN21AE9531 5590 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3400 278
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2933 1937
Size: 996 4594
Acc65I G'GTAC_C
Cuts at: 2911 2911
Size: 5590
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3653 5037 5305 1632
Size: 2021 1384 268 1917
Fragments arranged by size:
2021 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5393
AflIII A'CryG_T
Cuts at: 1203 4489 5466 1203
Size: 3286 977 1327
Fragments arranged by size:
3286 1327 977
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5590
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5590
AlwNI CAG_nnn'CTG
Cuts at: 2256 2325 2623 3333 4905 2256
Size: 69 298 710 1572 2941
Fragments arranged by size:
2941 1572 710 298 69
ApaI G_GGCC'C
Cuts at: 2693 2693
Size: 5590
ApaLI G'TGCA_C
Cuts at: 3647 4803 3647
Size: 1156 4434
ApoI r'AATT_y
Cuts at: 1493 2899 3125 3277 5386 1493
Size: 1406 226 152 2109 1697
Fragments arranged by size:
2109 1697 1406 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2915 1870
Size: 73 102 870 4545
Fragments arranged by size:
4545 870 102 73
BamHI G'GATC_C
Cuts at: 2920 3287 2920
Size: 367 5223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2761 2785 2911 4366 618
Size: 325 217 627 974 24 126 1455 1842
Fragments arranged by size:
1842 1455 974 627 325 217 126 24
BanII G_rGCy'C
Cuts at: 1391 2046 2693 2909 1391
Size: 655 647 216 4072
Fragments arranged by size:
4072 655 647 216
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2421 928
Size: 971 522 4097
Fragments arranged by size:
4097 971 522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3781 3815 1903
Size: 34 1844 34 3678
Fragments arranged by size:
3678 1844 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3482 4692 3482
Size: 1210 4380
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5590
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2441 4201 136
Size: 107 122 71 631 605 769 1760 1525
Fragments arranged by size:
1760 1525 769 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5585 5585
Size: 5590
BlpI GC'TnA_GC
Cuts at: 2171 3007 2171
Size: 836 4754
Bme1580I G_kGCm'C
Cuts at: 1790 2693 2766 2790 3651 4807 1790
Size: 903 73 24 861 1156 2573
Fragments arranged by size:
2573 1156 903 861 73 24
BmgBI CAC'GTC
Cuts at: 1318 2731 1318
Size: 1413 4177
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2498 2633 3321
Size: 416 53 83 399 24 1081 135 688
Cuts at: 3321 4279 442
Size: 958 1753
Fragments arranged by size:
1753 1081 958 688 416 399 135 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2196 2196
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 4250 1239
Size: 1388 1623 2579
Fragments arranged by size:
2579 1623 1388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3715 4580 4878 3715
Size: 865 298 4427
Fragments arranged by size:
4427 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2006 2099 4253 882
Size: 617 507 93 2154 2219
Fragments arranged by size:
2219 2154 617 507 93
BsaAI yAC'GTr
Cuts at: 493 1204 3381 493
Size: 711 2177 2702
Fragments arranged by size:
2702 2177 711
BsaBI GATnn'nnATC
Cuts at: 5584 5584
Size: 5590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3397 3779 4414 275
Size: 53 83 186 936 1864 382 635 1451
Fragments arranged by size:
1864 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2090 4023 4421 4695 4842 5253 2090
Size: 1933 398 274 147 411 2427
Fragments arranged by size:
2427 1933 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5365
Fragments arranged by size:
5365 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5590
BseYI C'CCAG_C
Cuts at: 2685 4793 5289 2685
Size: 2108 496 2986
Fragments arranged by size:
2986 2108 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2353 2355 2485 1163
Size: 573 617 2 130 4268
Fragments arranged by size:
4268 617 573 130 2
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3040 3801 4829 1540
Size: 275 186 1039 761 1028 2301
Fragments arranged by size:
2301 1039 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2415 3651 3736 4807 2046
Size: 369 1236 85 1071 2829
Fragments arranged by size:
2829 1236 1071 369 85
BsmI GAATG_Cn'
Cuts at: 2212 3101 3194 2212
Size: 889 93 4608
Fragments arranged by size:
4608 889 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2407 2407
Size: 5590
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2415 2693 2766 2790 2909 3651
Size: 399 256 369 278 73 24 119 742
Cuts at: 3651 3736 4807 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 278 256 119
85 73 24
BspEI T'CCGG_A
Cuts at: 5253 5253
Size: 5590
BspHI T'CATG_A
Cuts at: 3479 5209 3479
Size: 1730 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2945 844
Size: 2101 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3477 1394
Size: 429 1654 3507
Fragments arranged by size:
3507 1654 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2772 2827 4085 4259 65
Size: 1183 1524 55 1258 174 1396
Fragments arranged by size:
1524 1396 1258 1183 174 55
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 4234 4421 1700
Size: 110 220 2204 187 2869
Fragments arranged by size:
2869 2204 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5590
BssSI C'ACGA_G
Cuts at: 3650 4662 3650
Size: 1012 4578
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2761 794
Size: 1967 3623
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5590
BstYI r'GATC_y
Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585
Size: 948 367 385 17 1452 86 12 346
Cuts at: 5585 1972
Size: 1977
Fragments arranged by size:
1977 1452 948 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4099
Fragments arranged by size:
4099 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2326 2707 487
Size: 683 56 1100 381 3370
Fragments arranged by size:
3370 1100 683 381 56
BtsI GCAGTG_nn'
Cuts at: 2338 2855 3106 3900 3920 2338
Size: 517 251 794 20 4008
Fragments arranged by size:
4008 794 517 251 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5555
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 809
Size: 507 3281 1802
Fragments arranged by size:
3281 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2316 2635 3037
Size: 54 1425 50 275 18 486 319 402
Cuts at: 3037 3926 5555 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 486 402 319 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3037 1537
Size: 275 1225 4090
Fragments arranged by size:
4090 1225 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2420 2552 2588 2624 2861 3520 1353
Size: 1067 132 36 36 237 659 3423
Fragments arranged by size:
3423 1067 659 237 132 36 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4177 4551 4697 387
Size: 1064 138 2588 374 146 1280
Fragments arranged by size:
2588 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2044 2044
Size: 5590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2627 3653 4250 5037 5305 1239
Size: 393 995 1026 597 787 268 1524
Fragments arranged by size:
1524 1026 995 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2689 2690 2689
Size: 1 5589
EcoRI G'AATT_C
Cuts at: 2899 3277 2899
Size: 378 5212
FspI TGC'GCA
Cuts at: 4096 4096
Size: 5590
HaeII r_GCGC'y
Cuts at: 1678 2257 4737 1678
Size: 579 2480 2531
Fragments arranged by size:
2531 2480 579
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2623 2655 4231 4263 4305
Size: 32 135 32 1051 32 1576 32 42
Cuts at: 4305 4337 1373
Size: 32 2626
Fragments arranged by size:
2626 1576 1051 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2934 3180 669
Size: 988 281 996 246 3079
Fragments arranged by size:
3079 996 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5590
HpaI GTT'AAC
Cuts at: 3180 3180
Size: 5590
KpnI G_GTAC'C
Cuts at: 2915 2915
Size: 5590
MfeI C'AATT_G
Cuts at: 3189 3189
Size: 5590
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4704 4888 1305
Size: 3399 184 2007
Fragments arranged by size:
3399 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2318 2637 10
Size: 54 1425 343 486 319 2963
Fragments arranged by size:
2963 1425 486 343 319 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3548 3907 4066 518
Size: 648 2382 359 159 2042
Fragments arranged by size:
2382 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2383 3006 3683 4831 5076 1606
Size: 777 623 677 1148 245 2120
Fragments arranged by size:
2120 1148 777 677 623 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4099
Fragments arranged by size:
4099 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2499 2948 4493 1132
Size: 97 1270 449 1545 2229
Fragments arranged by size:
2229 1545 1270 449 97
PasI CC'CwG_GG
Cuts at: 1348 2815 1348
Size: 1467 4123
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2164 2346 1108
Size: 1056 182 4352
Fragments arranged by size:
4352 1056 182
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5172 5204 3659
Size: 32 1481 32 4045
Fragments arranged by size:
4045 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 3160 3160
Size: 5590
PspOMI G'GGCC_C
Cuts at: 2689 2689
Size: 5590
PspXI vC'TCGA_Gb
Cuts at: 2045 2045
Size: 5590
PstI C_TGCA'G
Cuts at: 830 2330 2757 2837 2942 830
Size: 1500 427 80 105 3478
Fragments arranged by size:
3478 1500 427 105 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5590
PvuII CAG'CTG
Cuts at: 2383 2383
Size: 5590
SacI G_AGCT'C
Cuts at: 2046 2046
Size: 5590
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SapI GCTCTTCn'nnn_
Cuts at: 2420 2420
Size: 5590
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 3838 5550 3838
Size: 1712 3878
SfcI C'TryA_G
Cuts at: 826 1045 2326 2602 2753 2833 2938 4073 4754
Size: 219 1281 276 151 80 105 1135 681
Cuts at: 4754 4945 826
Size: 191 1471
Fragments arranged by size:
1471 1281 1135 681 276 219 191 151
105 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2917 1872
Size: 1045 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 3694 4595 4857 820
Size: 197 926 102 1649 901 262 1553
Fragments arranged by size:
1649 1553 926 901 262 197 102
SnaBI TAC'GTA
Cuts at: 493 3381 493
Size: 2888 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2948 1132
Size: 1816 3774
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5590
StyI C'CwwG_G
Cuts at: 513 1065 2004 2517 2886 3029 513
Size: 552 939 513 369 143 3074
Fragments arranged by size:
3074 939 552 513 369 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2895 3617 3634 3787 3972 4417 325
Size: 1255 1315 722 17 153 185 445 1498
Fragments arranged by size:
1498 1315 1255 722 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3836 5548 96
Size: 274 80 33 51 1443 1859 1712 138
Fragments arranged by size:
1859 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5550 5582 5550
Size: 32 5558
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2755 2755
Size: 5590
XhoI C'TCGA_G
Cuts at: 1943 2045 1943
Size: 102 5488
XmaI C'CCGG_G
Cuts at: 1870 2915 1870
Size: 1045 4545
XmnI GAAnn'nnTTC
Cuts at: 3719 3719
Size: 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3398 276
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI