Restriction map for
pFN21AE9527
(Circular) (Six-base) MAPSORT of: pFN21AE9527.seq Check: 8577 from: 1 to: 5896
pFN21AE9527 5896 bp
With 182 enzymes: SgfI *
August 25, 2016 14:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3706 278
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2247 3239 1937
Size: 310 992 4594
Fragments arranged by size:
4594 992 310
Acc65I G'GTAC_C
Cuts at: 3217 3217
Size: 5896
AclI AA'CG_TT
Cuts at: 1563 4023 4396 1563
Size: 2460 373 3063
Fragments arranged by size:
3063 2460 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3959 5343 5611 1632
Size: 2327 1384 268 1917
Fragments arranged by size:
2327 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5699
AflIII A'CryG_T
Cuts at: 1203 2478 2718 4795 5772 1203
Size: 1275 240 2077 977 1327
Fragments arranged by size:
2077 1327 1275 977 240
AgeI A'CCGG_T
Cuts at: 4727 4727
Size: 5896
AhdI GACnn_n'nnGTC
Cuts at: 4625 4625
Size: 5896
AleI CACnn'nnGTG
Cuts at: 1166 2255 1166
Size: 1089 4807
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5896
AlwNI CAG_nnn'CTG
Cuts at: 2783 3639 5211 2783
Size: 856 1572 3468
Fragments arranged by size:
3468 1572 856
ApaLI G'TGCA_C
Cuts at: 3953 5109 3953
Size: 1156 4740
ApoI r'AATT_y
Cuts at: 1493 3205 3431 3583 5692 1493
Size: 1712 226 152 2109 1697
Fragments arranged by size:
2109 1712 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4450 160
Size: 872 3418 1606
Fragments arranged by size:
3418 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
AvaI C'yCGr_G
Cuts at: 1870 1943 3221 1870
Size: 73 1278 4545
Fragments arranged by size:
4545 1278 73
BamHI G'GATC_C
Cuts at: 3226 3593 3226
Size: 367 5529
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 3217 4672 618
Size: 325 217 627 288 1142 1455 1842
Fragments arranged by size:
1842 1455 1142 627 325 288 217
BanII G_rGCy'C
Cuts at: 1391 2015 3215 1391
Size: 624 1200 4072
Fragments arranged by size:
4072 1200 624
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 928
Size: 971 325 4600
Fragments arranged by size:
4600 971 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4087 4121 1903
Size: 34 50 34 2066 34 3678
Fragments arranged by size:
3678 2066 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3130 3788 4998 3130
Size: 658 1210 4028
Fragments arranged by size:
4028 1210 658
BclI T'GATC_A
Cuts at: 1548 2799 1548
Size: 1251 4645
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2354 4507 136
Size: 107 122 71 631 605 682 2153 1525
Fragments arranged by size:
2153 1525 682 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5891 5891
Size: 5896
BlpI GC'TnA_GC
Cuts at: 3313 3313
Size: 5896
Bme1580I G_kGCm'C
Cuts at: 1790 2553 3957 5113 1790
Size: 763 1404 1156 2573
Fragments arranged by size:
2573 1404 1156 763
BmgBI CAC'GTC
Cuts at: 1318 2146 1318
Size: 828 5068
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3627 4585 442
Size: 416 53 83 399 24 2210 958 1753
Fragments arranged by size:
2210 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2054 1056
Size: 998 4898
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2539 3127 4556 1239
Size: 1300 588 1429 2579
Fragments arranged by size:
2579 1429 1300 588
Bpu10I CC'TnA_GC
Cuts at: 2697 2697
Size: 5896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4021 4886 5184 4021
Size: 865 298 4733
Fragments arranged by size:
4733 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4559 882
Size: 617 3060 2219
Fragments arranged by size:
3060 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3687 493
Size: 711 2483 2702
Fragments arranged by size:
2702 2483 711
BsaBI GATnn'nnATC
Cuts at: 5890 5890
Size: 5896
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3703 4085 4720 275
Size: 53 83 186 936 2170 382 635 1451
Fragments arranged by size:
2170 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4329 4727 5001 5148 5559 4329
Size: 398 274 147 411 4666
Fragments arranged by size:
4666 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2151 2181 1178
Size: 30 165 30 748 30 4893
Fragments arranged by size:
4893 748 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2360 1186
Size: 1174 4722
BseYI C'CCAG_C
Cuts at: 2015 2032 2764 5099 5595 2015
Size: 17 732 2335 496 2316
Fragments arranged by size:
2335 2316 732 496 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2537 1163
Size: 573 801 4522
Fragments arranged by size:
4522 801 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3346 4107 5135 1540
Size: 275 186 1345 761 1028 2301
Fragments arranged by size:
2301 1345 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3957 4042 5113 3957
Size: 85 1071 4740
Fragments arranged by size:
4740 1071 85
BsmI GAATG_Cn'
Cuts at: 2811 3407 3500 2811
Size: 596 93 5207
Fragments arranged by size:
5207 596 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2553 3215 3957 4042 5113 1391
Size: 399 225 538 662 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 662 538 399 225 85
BspEI T'CCGG_A
Cuts at: 5559 5559
Size: 5896
BspHI T'CATG_A
Cuts at: 2499 2683 3785 5515 2499
Size: 184 1102 1730 2880
Fragments arranged by size:
2880 1730 1102 184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2513 3251 844
Size: 1669 738 3489
Fragments arranged by size:
3489 1669 738
BsrBI CCG'CTC
Cuts at: 1394 1823 3783 1394
Size: 429 1960 3507
Fragments arranged by size:
3507 1960 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2571 4391 4565 65
Size: 1183 1323 1820 174 1396
Fragments arranged by size:
1820 1396 1323 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4540 4727 1700
Size: 110 2730 187 2869
Fragments arranged by size:
2869 2730 187 110
BsrGI T'GTAC_A
Cuts at: 96 2979 96
Size: 2883 3013
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5896
BssSI C'ACGA_G
Cuts at: 3956 4968 3956
Size: 1012 4884
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2573 794
Size: 1779 4117
BstBI TT'CG_AA
Cuts at: 4700 4700
Size: 5896
BstEII G'GTnAC_C
Cuts at: 1184 2617 1184
Size: 1433 4463
BstXI CCAn_nnnn'nTGG
Cuts at: 2150 2150
Size: 5896
BstYI r'GATC_y
Cuts at: 1972 2096 3226 3593 3978 3995 5447 5533 5545
Size: 124 1130 367 385 17 1452 86 12
Cuts at: 5545 5891 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1130 385 367 346 124 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2544 2823 513
Size: 552 1479 279 3586
Fragments arranged by size:
3586 1479 552 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5157
Fragments arranged by size:
5157 683 56
BtsI GCAGTG_nn'
Cuts at: 2077 3412 4206 4226 2077
Size: 1335 794 20 3747
Fragments arranged by size:
3747 1335 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5896
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5861
DraI TTT'AAA
Cuts at: 3200 3200
Size: 5896
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3029 4903 809
Size: 507 1713 1874 1802
Fragments arranged by size:
1874 1802 1713 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2346 2742 2883
Size: 54 1425 50 275 18 516 396 141
Cuts at: 2883 3343 4232 5861 8
Size: 460 889 1629 43
Fragments arranged by size:
1629 1425 889 516 460 396 275 141
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3343 1537
Size: 275 1531 4090
Fragments arranged by size:
4090 1531 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2501 2846 3169 3826 1353
Size: 1148 345 323 657 3423
Fragments arranged by size:
3423 1148 657 345 323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4483 4857 5003 387
Size: 1064 138 2894 374 146 1280
Fragments arranged by size:
2894 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2539 3127 3959 4556 5343 5611 1239
Size: 393 907 588 832 597 787 268 1524
Fragments arranged by size:
1524 907 832 787 597 588 393 268
EcoRI G'AATT_C
Cuts at: 3205 3583 3205
Size: 378 5518
FspI TGC'GCA
Cuts at: 4402 4402
Size: 5896
HaeII r_GCGC'y
Cuts at: 1678 5043 1678
Size: 3365 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2123 2155 2357 2389 2421
Size: 32 135 32 551 32 202 32 32
Cuts at: 2421 2819 2851 4537 4569 4611 4643 1373
Size: 398 32 1686 32 42 32 2626
Fragments arranged by size:
2626 1686 551 398 202 135 42 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3240 3486 669
Size: 988 281 1302 246 3079
Fragments arranged by size:
3079 1302 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5896
HpaI GTT'AAC
Cuts at: 3486 3486
Size: 5896
KpnI G_GTAC'C
Cuts at: 3221 3221
Size: 5896
MfeI C'AATT_G
Cuts at: 2241 2253 3495 2241
Size: 12 1242 4642
Fragments arranged by size:
4642 1242 12
MluI A'CGCG_T
Cuts at: 5772 5772
Size: 5896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5010 5194 1305
Size: 3705 184 2007
Fragments arranged by size:
3705 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2348 2744 2885 10
Size: 54 1425 343 516 396 141 3021
Fragments arranged by size:
3021 1425 516 396 343 141 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2412 2970 3854 4213 4372 518
Size: 648 1089 157 558 884 359 159 2042
Fragments arranged by size:
2042 1089 884 648 558 359 159 157
MspA1I CmG'CkG
Cuts at: 1606 3015 3312 3989 5137 5382 1606
Size: 1409 297 677 1148 245 2120
Fragments arranged by size:
2120 1409 1148 677 297 245
NcoI C'CATG_G
Cuts at: 513 1065 2544 2823 513
Size: 552 1479 279 3586
Fragments arranged by size:
3586 1479 552 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5896
NheI G'CTAG_C
Cuts at: 1052 2050 1052
Size: 998 4898
NotI GC'GGCC_GC
Cuts at: 3343 3343
Size: 5896
NspI r_CATG'y
Cuts at: 1132 1229 2482 2722 3254 4799 1132
Size: 97 1253 240 532 1545 2229
Fragments arranged by size:
2229 1545 1253 532 240 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5896
PciI A'CATG_T
Cuts at: 2478 2718 4795 2478
Size: 240 2077 3579
Fragments arranged by size:
3579 2077 240
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5896
PfoI T'CCnGG_A
Cuts at: 2555 3143 2555
Size: 588 5308
PmeI GTTT'AAAC
Cuts at: 3200 3200
Size: 5896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3965 3997 5478 5510 3965
Size: 32 1481 32 4351
Fragments arranged by size:
4351 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2228 1613
Size: 615 5281
PsiI TTA'TAA
Cuts at: 3466 3466
Size: 5896
PstI C_TGCA'G
Cuts at: 830 2072 3248 830
Size: 1242 1176 3478
Fragments arranged by size:
3478 1242 1176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5896
PvuII CAG'CTG
Cuts at: 3015 3015
Size: 5896
SalI G'TCGA_C
Cuts at: 1936 3238 1936
Size: 1302 4594
SbfI CC_TGCA'GG
Cuts at: 3248 3248
Size: 5896
ScaI AGT'ACT
Cuts at: 4144 5856 4144
Size: 1712 4184
SfcI C'TryA_G
Cuts at: 826 1045 2068 3244 4379 5060 5251 826
Size: 219 1023 1176 1135 681 191 1471
Fragments arranged by size:
1471 1176 1135 1023 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2354 2354
Size: 5896
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5896
SmaI CCC'GGG
Cuts at: 1872 3223 1872
Size: 1351 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4000 4901 5163 820
Size: 197 926 2057 901 262 1553
Fragments arranged by size:
2057 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3687 493
Size: 3194 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5896
SphI G_CATG'C
Cuts at: 1132 3254 1132
Size: 2122 3774
SspI AAT'ATT
Cuts at: 5 52 3820 5
Size: 47 3768 2081
Fragments arranged by size:
3768 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5896
StyI C'CwwG_G
Cuts at: 513 1065 2083 2358 2544 2823 3335 513
Size: 552 1018 275 186 279 512 3074
Fragments arranged by size:
3074 1018 552 512 279 275 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3923 3940 4093 4278 4723 325
Size: 1255 2343 17 153 185 445 1498
Fragments arranged by size:
2343 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2481 2979 4142
Size: 274 80 33 51 1443 504 498 1163
Cuts at: 4142 5854 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1163 504 498 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5856 5888 5856
Size: 32 5864
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5896
XbaI T'CTAG_A
Cuts at: 3232 3232
Size: 5896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2356 2356
Size: 5896
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5896
XmaI C'CCGG_G
Cuts at: 1870 3221 1870
Size: 1351 4545
XmnI GAAnn'nnTTC
Cuts at: 4025 4025
Size: 5896
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3704 276
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfoI SrfI SwaI