Restriction map for
pFN21AE9522
(Circular) (Six-base) MAPSORT of: pFN21AE9522.seq Check: 1881 from: 1 to: 7321
pFN21AE9522 7321 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2244 4319 2244
Size: 2075 5246
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5131 278
Size: 53 83 186 936 3595 2468
Fragments arranged by size:
3595 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4664 1937
Size: 2727 4594
Acc65I G'GTAC_C
Cuts at: 4642 4642
Size: 7321
AclI AA'CG_TT
Cuts at: 1563 5448 5821 1563
Size: 3885 373 3063
Fragments arranged by size:
3885 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5384 6768 7036 1632
Size: 3752 1384 268 1917
Fragments arranged by size:
3752 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7124
AflIII A'CryG_T
Cuts at: 1203 6220 7197 1203
Size: 5017 977 1327
Fragments arranged by size:
5017 1327 977
AgeI A'CCGG_T
Cuts at: 6152 6152
Size: 7321
AhdI GACnn_n'nnGTC
Cuts at: 6050 6050
Size: 7321
AleI CACnn'nnGTG
Cuts at: 1166 2876 3791 3893 1166
Size: 1710 915 102 4594
Fragments arranged by size:
4594 1710 915 102
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7321
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 7289
AlwNI CAG_nnn'CTG
Cuts at: 2188 2311 3004 5064 6636 2188
Size: 123 693 2060 1572 2873
Fragments arranged by size:
2873 2060 1572 693 123
ApaI G_GGCC'C
Cuts at: 2066 2902 3997 2066
Size: 836 1095 5390
Fragments arranged by size:
5390 1095 836
ApaLI G'TGCA_C
Cuts at: 2123 2259 5378 6534 2123
Size: 136 3119 1156 2910
Fragments arranged by size:
3119 2910 1156 136
ApoI r'AATT_y
Cuts at: 1493 3044 3605 4630 4856 5008 7117 1493
Size: 1551 561 1025 226 152 2109 1697
Fragments arranged by size:
2109 1697 1551 1025 561 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5875 160
Size: 872 4843 1606
Fragments arranged by size:
4843 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7321
AvaI C'yCGr_G
Cuts at: 1870 1943 2829 3996 4066 4646 1870
Size: 73 886 1167 70 580 4545
Fragments arranged by size:
4545 1167 886 580 73 70
BamHI G'GATC_C
Cuts at: 4651 5018 4651
Size: 367 6954
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3249 3737 4004 4108 4492
Size: 325 217 627 1462 488 267 104 384
Cuts at: 4492 4642 6097 618
Size: 150 1455 1842
Fragments arranged by size:
1842 1462 1455 627 488 384 325 267
217 150 104
BanII G_rGCy'C
Cuts at: 1391 2066 2773 2902 3513 3819 3997 4196 4640
Size: 675 707 129 611 306 178 199 444
Cuts at: 4640 1391
Size: 4072
Fragments arranged by size:
4072 707 675 611 444 306 199 178
129
BbeI G_GCGC'C
Cuts at: 3253 3253
Size: 7321
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3835 928
Size: 971 1936 4414
Fragments arranged by size:
4414 1936 971
BbvCI CC'TCA_GC
Cuts at: 2058 2937 3475 4403 2058
Size: 879 538 928 4976
Fragments arranged by size:
4976 928 879 538
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4047 4081 5512 5546 1903
Size: 34 2110 34 1431 34 3678
Fragments arranged by size:
3678 2110 1431 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5213 6423 5213
Size: 1210 6111
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7321
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2373 2798 3657
Size: 107 122 71 631 605 701 425 859
Cuts at: 3657 4407 4509 5932 136
Size: 750 102 1423 1525
Fragments arranged by size:
1525 1423 859 750 701 631 605 425
122 107 102 71
BglII A'GATC_T
Cuts at: 7316 7316
Size: 7321
BlpI GC'TnA_GC
Cuts at: 4738 4738
Size: 7321
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2127 2263 2695 2794 2902 3117 3199
Size: 276 61 136 432 99 108 215 82
Cuts at: 3199 3742 3926 3997 4009 4118 4262 4495 5382
Size: 543 184 71 12 109 144 233 887
Cuts at: 5382 6538 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 887 543 432 276 233 215
184 144 136 109 108 99 82 71
61 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7321
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2114 2781 3433
Size: 416 53 83 399 24 697 667 652
Cuts at: 3433 4555 5052 6010 442
Size: 1122 497 958 1753
Fragments arranged by size:
1753 1122 958 697 667 652 497 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7321
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2980 2980
Size: 7321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2210 2321 2924 3503 4339 4519 5981
Size: 869 102 111 603 579 836 180 1462
Cuts at: 5981 1239
Size: 2579
Fragments arranged by size:
2579 1462 869 836 603 579 180 111
102
Bpu10I CC'TnA_GC
Cuts at: 2058 2937 3475 4403 2058
Size: 879 538 928 4976
Fragments arranged by size:
4976 928 879 538
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2488 5446 6311 6609 2488
Size: 2958 865 298 3200
Fragments arranged by size:
3200 2958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5984 882
Size: 617 4485 2219
Fragments arranged by size:
4485 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5112 493
Size: 711 3908 2702
Fragments arranged by size:
3908 2702 711
BsaBI GATnn'nnATC
Cuts at: 7315 7315
Size: 7321
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2636 3250 3910 5128
Size: 53 83 186 936 1103 614 660 1218
Cuts at: 5128 5510 6145 275
Size: 382 635 1451
Fragments arranged by size:
1451 1218 1103 936 660 635 614 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5754 6152 6426 6573 6984 5754
Size: 398 274 147 411 6091
Fragments arranged by size:
6091 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2085 2115 2264 2294 2545
Size: 30 165 30 682 30 149 30 251
Cuts at: 2545 2575 2972 3002 3044 3074 1178
Size: 30 397 30 42 30 5425
Fragments arranged by size:
5425 682 397 251 165 149 42 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4205 1186
Size: 3019 4302
BseYI C'CCAG_C
Cuts at: 2065 2104 2140 3304 3690 3760 3832 4008 6524
Size: 39 36 1164 386 70 72 176 2516
Cuts at: 6524 7020 2065
Size: 496 2366
Fragments arranged by size:
2516 2366 1164 496 386 176 72 70
39 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3405 3560 4213 4430 1163
Size: 573 1669 155 653 217 4054
Fragments arranged by size:
4054 1669 653 573 217 155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4771 5532 6560 1540
Size: 275 186 2770 761 1028 2301
Fragments arranged by size:
2770 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 2172 2263 3276 3819 4215 5382 5467 6538
Size: 45 91 1013 543 396 1167 85 1071
Cuts at: 6538 2127
Size: 2910
Fragments arranged by size:
2910 1167 1071 1013 543 396 91 85
45
BsmI GAATG_Cn'
Cuts at: 3570 4328 4832 4925 3570
Size: 758 504 93 5966
Fragments arranged by size:
5966 758 504 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2127 2172 2263 2695 2773 2794
Size: 399 276 61 45 91 432 78 21
Cuts at: 2794 2902 3117 3199 3276 3513 3742 3819 3926
Size: 108 215 82 77 237 229 77 107
Cuts at: 3926 3997 4009 4118 4196 4215 4262 4495 4640
Size: 71 12 109 78 19 47 233 145
Cuts at: 4640 5382 5467 6538 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 432 399 276 237 233
229 215 145 109 108 107 91 85
82 78 78 77 77 71 61 47
45 21 19 12
BspEI T'CCGG_A
Cuts at: 6984 6984
Size: 7321
BspHI T'CATG_A
Cuts at: 5210 6940 5210
Size: 1730 5591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 4319 4676 844
Size: 1400 2075 357 3489
Fragments arranged by size:
3489 2075 1400 357
BsrBI CCG'CTC
Cuts at: 1394 1823 5208 1394
Size: 429 3385 3507
Fragments arranged by size:
3507 3385 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3873 3972 4588 5816 5990 65
Size: 1183 2625 99 616 1228 174 1396
Fragments arranged by size:
2625 1396 1228 1183 616 174 99
BsrFI r'CCGG_y
Cuts at: 1700 1810 3479 5965 6152 1700
Size: 110 1669 2486 187 2869
Fragments arranged by size:
2869 2486 1669 187 110
BsrGI T'GTAC_A
Cuts at: 96 4302 96
Size: 4206 3115
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7321
BssSI C'ACGA_G
Cuts at: 2688 5381 6393 2688
Size: 2693 1012 3616
Fragments arranged by size:
3616 2693 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2239 3576 794
Size: 1445 1337 4539
Fragments arranged by size:
4539 1445 1337
BstBI TT'CG_AA
Cuts at: 3666 6125 3666
Size: 2459 4862
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7321
BstXI CCAn_nnnn'nTGG
Cuts at: 2455 2587 2959 3793 4054 4553 2455
Size: 132 372 834 261 499 5223
Fragments arranged by size:
5223 834 499 372 261 132
BstYI r'GATC_y
Cuts at: 1972 2481 4651 5018 5403 5420 6872 6958 6970
Size: 509 2170 367 385 17 1452 86 12
Cuts at: 6970 7316 1972
Size: 346 1977
Fragments arranged by size:
2170 1977 1452 509 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3805 3805
Size: 7321
BtgI C'CryG_G
Cuts at: 513 1065 2004 2173 2338 2389 2586 3165 3461
Size: 552 939 169 165 51 197 579 296
Cuts at: 3461 3888 4413 513
Size: 427 525 3421
Fragments arranged by size:
3421 939 579 552 525 427 296 197
169 165 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3245 3709 3711 4191 4204 4599
Size: 683 56 2019 464 2 480 13 395
Cuts at: 4599 487
Size: 3209
Fragments arranged by size:
3209 2019 683 480 464 395 56 13
2
BtsI GCAGTG_nn'
Cuts at: 2030 2403 2929 3162 3397 3511 3900 3924 4157
Size: 373 526 233 235 114 389 24 233
Cuts at: 4157 4519 4837 5631 5651 2030
Size: 362 318 794 20 3700
Fragments arranged by size:
3700 794 526 389 373 362 318 235
233 233 114 24 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7321
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3736 3771 529
Size: 35 3172 35 4079
Fragments arranged by size:
4079 3172 35 35
DraI TTT'AAA
Cuts at: 4625 4625
Size: 7321
DraIII CAC_nnn'GTG
Cuts at: 4386 4386
Size: 7321
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2623 6328 809
Size: 507 1307 3705 1802
Fragments arranged by size:
3705 1802 1307 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2457 2709 2880
Size: 54 1425 50 275 18 627 252 171
Cuts at: 2880 3236 3355 3744 4013 4044 4543 4585 4768
Size: 356 119 389 269 31 499 42 183
Cuts at: 4768 5657 7286 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 627 499 389 356 275
269 252 183 171 119 54 50 43
42 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 4768 1537
Size: 275 2956 4090
Fragments arranged by size:
4090 2956 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3527 4463 5251 1353
Size: 2174 936 788 3423
Fragments arranged by size:
3423 2174 936 788
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5908 6282 6428 387
Size: 1064 138 4319 374 146 1280
Fragments arranged by size:
4319 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3817 3817
Size: 7321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2210 2321 2924 3503 4339 4519
Size: 393 476 102 111 603 579 836 180
Cuts at: 4519 5384 5981 6768 7036 1239
Size: 865 597 787 268 1524
Fragments arranged by size:
1524 865 836 787 603 597 579 476
393 268 180 111 102
EcoNI CCTnn'n_nnAGG
Cuts at: 3260 3260
Size: 7321
EcoO109I rG'GnC_Cy
Cuts at: 2062 2898 2899 3713 3993 2062
Size: 836 1 814 280 5390
Fragments arranged by size:
5390 836 814 280 1
EcoRI G'AATT_C
Cuts at: 3044 3605 4630 5008 3044
Size: 561 1025 378 5357
Fragments arranged by size:
5357 1025 561 378
FspI TGC'GCA
Cuts at: 3091 5827 3091
Size: 2736 4585
HaeII r_GCGC'y
Cuts at: 1678 2188 2857 3253 3400 6468 1678
Size: 510 669 396 147 3068 2531
Fragments arranged by size:
3068 2531 669 510 396 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 2972 3004 3113
Size: 32 135 32 692 32 676 32 109
Cuts at: 3113 3145 5962 5994 6036 6068 1373
Size: 32 2817 32 42 32 2626
Fragments arranged by size:
2817 2626 692 676 135 109 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3244 4665 4911 669
Size: 988 281 1306 1421 246 3079
Fragments arranged by size:
3079 1421 1306 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7321
HpaI GTT'AAC
Cuts at: 3244 4911 3244
Size: 1667 5654
KasI G'GCGC_C
Cuts at: 3249 3249
Size: 7321
KpnI G_GTAC'C
Cuts at: 4646 4646
Size: 7321
MfeI C'AATT_G
Cuts at: 4920 4920
Size: 7321
MluI A'CGCG_T
Cuts at: 7197 7197
Size: 7321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6435 6619 1305
Size: 5130 184 2007
Fragments arranged by size:
5130 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2459 2711 2882 3746 4046
Size: 54 1425 343 627 252 171 864 300
Cuts at: 4046 4545 10
Size: 499 2786
Fragments arranged by size:
2786 1425 864 627 499 343 300 252
171 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2394 2453 2705 2876 3143 3791 3893
Size: 648 1228 59 252 171 267 648 102
Cuts at: 3893 4176 4382 4388 5279 5638 5797 518
Size: 283 206 6 891 359 159 2042
Fragments arranged by size:
2042 1228 891 648 648 359 283 267
252 206 171 159 102 59 6
MspA1I CmG'CkG
Cuts at: 1606 3066 3308 3690 4012 4075 4407 4737 5414
Size: 1460 242 382 322 63 332 330 677
Cuts at: 5414 6562 6807 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1460 1148 677 382 332 330 322
245 242 63
NaeI GCC'GGC
Cuts at: 3481 3481
Size: 7321
NarI GG'CG_CC
Cuts at: 3250 3250
Size: 7321
NcoI C'CATG_G
Cuts at: 513 1065 2004 2389 2586 3165 3461 513
Size: 552 939 385 197 579 296 4373
Fragments arranged by size:
4373 939 579 552 385 296 197
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7321
NgoMIV G'CCGG_C
Cuts at: 3479 3479
Size: 7321
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7321
NotI GC'GGCC_GC
Cuts at: 4768 4768
Size: 7321
NspI r_CATG'y
Cuts at: 1132 1229 2297 3190 4679 6224 1132
Size: 97 1068 893 1489 1545 2229
Fragments arranged by size:
2229 1545 1489 1068 893 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7321
PciI A'CATG_T
Cuts at: 6220 6220
Size: 7321
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2188 3004 3415 1108
Size: 1080 816 411 5014
Fragments arranged by size:
5014 1080 816 411
PmeI GTTT'AAAC
Cuts at: 4625 4625
Size: 7321
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 4268 4300 5390 5422 6903 6935 3044
Size: 32 1192 32 1090 32 1481 32 3430
Fragments arranged by size:
3430 1481 1192 1090 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3713 3713
Size: 7321
PshAI GACnn'nnGTC
Cuts at: 1613 2154 1613
Size: 541 6780
PsiI TTA'TAA
Cuts at: 4891 4891
Size: 7321
PspOMI G'GGCC_C
Cuts at: 2062 2898 3993 2062
Size: 836 1095 5390
Fragments arranged by size:
5390 1095 836
PstI C_TGCA'G
Cuts at: 830 2781 3071 3506 4673 830
Size: 1951 290 435 1167 3478
Fragments arranged by size:
3478 1951 1167 435 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7321
PvuII CAG'CTG
Cuts at: 3308 4075 3308
Size: 767 6554
SacI G_AGCT'C
Cuts at: 3819 3819
Size: 7321
SalI G'TCGA_C
Cuts at: 1936 4663 1936
Size: 2727 4594
SbfI CC_TGCA'GG
Cuts at: 4673 4673
Size: 7321
ScaI AGT'ACT
Cuts at: 5569 7281 5569
Size: 1712 5609
SfcI C'TryA_G
Cuts at: 826 1045 2434 2777 3067 3502 4669 5804 6485
Size: 219 1389 343 290 435 1167 1135 681
Cuts at: 6485 6676 826
Size: 191 1471
Fragments arranged by size:
1471 1389 1167 1135 681 435 343 290
219 191
SfoI GGC'GCC
Cuts at: 3251 3251
Size: 7321
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7321
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7321
SmaI CCC'GGG
Cuts at: 1872 2831 3998 4648 1872
Size: 959 1167 650 4545
Fragments arranged by size:
4545 1167 959 650
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2467 5425 6326 6588 820
Size: 197 926 524 2958 901 262 1553
Fragments arranged by size:
2958 1553 926 901 524 262 197
SnaBI TAC'GTA
Cuts at: 493 5112 493
Size: 4619 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7321
SphI G_CATG'C
Cuts at: 1132 4679 1132
Size: 3547 3774
SspI AAT'ATT
Cuts at: 5 52 5245 5
Size: 47 5193 2081
Fragments arranged by size:
5193 2081 47
StuI AGG'CCT
Cuts at: 1517 2672 3803 1517
Size: 1155 1131 5035
Fragments arranged by size:
5035 1155 1131
StyI C'CwwG_G
Cuts at: 513 1065 2004 2283 2389 2580 2586 3011 3165
Size: 552 939 279 106 191 6 425 154
Cuts at: 3165 3461 4540 4546 4760 513
Size: 296 1079 6 214 3074
Fragments arranged by size:
3074 1079 939 552 425 296 279 214
191 154 106 6 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2096 2246 2866 3329 3752 3824 4510
Size: 1255 516 150 620 463 423 72 686
Cuts at: 4510 5348 5365 5518 5703 6148 325
Size: 838 17 153 185 445 1498
Fragments arranged by size:
1498 1255 838 686 620 516 463 445
423 185 153 150 72 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3681 4302 5567
Size: 274 80 33 51 1443 1704 621 1265
Cuts at: 5567 7279 96
Size: 1712 138
Fragments arranged by size:
1712 1704 1443 1265 621 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 2545 2577 7281 7313 2085
Size: 32 428 32 4704 32 2093
Fragments arranged by size:
4704 2093 428 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4609 1316
Size: 3293 4028
XbaI T'CTAG_A
Cuts at: 4657 4657
Size: 7321
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2794 2794
Size: 7321
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7321
XmaI C'CCGG_G
Cuts at: 1870 2829 3996 4646 1870
Size: 959 1167 650 4545
Fragments arranged by size:
4545 1167 959 650
XmnI GAAnn'nnTTC
Cuts at: 5450 5450
Size: 7321
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5129 276
Size: 53 83 186 936 3595 2468
Fragments arranged by size:
3595 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI