Restriction map for
pFN21AE9514
(Circular) (Six-base) MAPSORT of: pFN21AE9514.seq Check: 6475 from: 1 to: 5890
pFN21AE9514 5890 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2552 2552
Size: 5890
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3700 278
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3233 1937
Size: 1296 4594
Acc65I G'GTAC_C
Cuts at: 3211 3211
Size: 5890
AclI AA'CG_TT
Cuts at: 1563 4017 4390 1563
Size: 2454 373 3063
Fragments arranged by size:
3063 2454 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3953 5337 5605 1632
Size: 2321 1384 268 1917
Fragments arranged by size:
2321 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5693
AflIII A'CryG_T
Cuts at: 1203 4789 5766 1203
Size: 3586 977 1327
Fragments arranged by size:
3586 1327 977
AgeI A'CCGG_T
Cuts at: 2141 4721 2141
Size: 2580 3310
AhdI GACnn_n'nnGTC
Cuts at: 4619 4619
Size: 5890
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5890
AlwNI CAG_nnn'CTG
Cuts at: 3135 3144 3633 5205 3135
Size: 9 489 1572 3820
Fragments arranged by size:
3820 1572 489 9
ApaLI G'TGCA_C
Cuts at: 3947 5103 3947
Size: 1156 4734
ApoI r'AATT_y
Cuts at: 1493 2314 2492 3199 3425 3577 5686 1493
Size: 821 178 707 226 152 2109 1697
Fragments arranged by size:
2109 1697 821 707 226 178 152
AseI AT'TA_AT
Cuts at: 160 1032 4444 160
Size: 872 3412 1606
Fragments arranged by size:
3412 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
AvaI C'yCGr_G
Cuts at: 1870 1943 3215 1870
Size: 73 1272 4545
Fragments arranged by size:
4545 1272 73
AvrII C'CTAG_G
Cuts at: 2582 2582
Size: 5890
BamHI G'GATC_C
Cuts at: 3220 3587 3220
Size: 367 5523
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2391 3211 4666 618
Size: 325 217 627 604 820 1455 1842
Fragments arranged by size:
1842 1455 820 627 604 325 217
BanII G_rGCy'C
Cuts at: 1391 2832 3209 1391
Size: 1441 377 4072
Fragments arranged by size:
4072 1441 377
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2551 928
Size: 971 652 4267
Fragments arranged by size:
4267 971 652
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2836 2870 4081 4115 1903
Size: 34 899 34 1211 34 3678
Fragments arranged by size:
3678 1211 899 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2052 2110 2351 3782 4992 2052
Size: 58 241 1431 1210 2950
Fragments arranged by size:
2950 1431 1210 241 58
BclI T'GATC_A
Cuts at: 1548 2568 1548
Size: 1020 4870
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4501 136
Size: 107 122 71 631 605 2829 1525
Fragments arranged by size:
2829 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5885 5885
Size: 5890
BlpI GC'TnA_GC
Cuts at: 3307 3307
Size: 5890
Bme1580I G_kGCm'C
Cuts at: 1790 3951 5107 1790
Size: 2161 1156 2573
Fragments arranged by size:
2573 2161 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3621 4579 442
Size: 416 53 83 399 24 2204 958 1753
Fragments arranged by size:
2204 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2575 4550 1239
Size: 1336 1975 2579
Fragments arranged by size:
2579 1975 1336
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2572 4015 4880 5178 2572
Size: 1443 865 298 3284
Fragments arranged by size:
3284 1443 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2150 2435 2639 4553 882
Size: 617 651 285 204 1914 2219
Fragments arranged by size:
2219 1914 651 617 285 204
BsaAI yAC'GTr
Cuts at: 493 1204 3681 493
Size: 711 2477 2702
Fragments arranged by size:
2702 2477 711
BsaBI GATnn'nnATC
Cuts at: 2573 5884 2573
Size: 3311 2579
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3697 4079 4714 275
Size: 53 83 186 936 2164 382 635 1451
Fragments arranged by size:
2164 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2141 4323 4721 4995 5142 5553 2141
Size: 2182 398 274 147 411 2478
Fragments arranged by size:
2478 2182 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5665
Fragments arranged by size:
5665 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5890
BseYI C'CCAG_C
Cuts at: 2652 3119 5093 5589 2652
Size: 467 1974 496 2953
Fragments arranged by size:
2953 1974 496 467
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2137 2223 3045 1163
Size: 573 401 86 822 4008
Fragments arranged by size:
4008 822 573 401 86
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3340 4101 5129 1540
Size: 275 186 1339 761 1028 2301
Fragments arranged by size:
2301 1339 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2972 3951 4036 5107 2972
Size: 979 85 1071 3755
Fragments arranged by size:
3755 1071 979 85
BsmI GAATG_Cn'
Cuts at: 2126 3401 3494 2126
Size: 1275 93 4522
Fragments arranged by size:
4522 1275 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2832 2972 3209 3951 4036 5107 1391
Size: 399 1042 140 237 742 85 1071 2174
Fragments arranged by size:
2174 1071 1042 742 399 237 140 85
BspEI T'CCGG_A
Cuts at: 5553 5553
Size: 5890
BspHI T'CATG_A
Cuts at: 3172 3779 5509 3172
Size: 607 1730 3553
Fragments arranged by size:
3553 1730 607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2552 2875 3245 844
Size: 1708 323 370 3489
Fragments arranged by size:
3489 1708 370 323
BsrBI CCG'CTC
Cuts at: 1394 1823 3777 1394
Size: 429 1954 3507
Fragments arranged by size:
3507 1954 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3069 4385 4559 65
Size: 1183 1821 1316 174 1396
Fragments arranged by size:
1821 1396 1316 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2141 4534 4721 1700
Size: 110 331 2393 187 2869
Fragments arranged by size:
2869 2393 331 187 110
BsrGI T'GTAC_A
Cuts at: 96 2868 96
Size: 2772 3118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5890
BssSI C'ACGA_G
Cuts at: 3950 4962 3950
Size: 1012 4878
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3144 794
Size: 2350 3540
BstBI TT'CG_AA
Cuts at: 4694 4694
Size: 5890
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5890
BstXI CCAn_nnnn'nTGG
Cuts at: 2266 2860 2266
Size: 594 5296
BstYI r'GATC_y
Cuts at: 1972 2369 3125 3220 3587 3972 3989 5441 5527
Size: 397 756 95 367 385 17 1452 86
Cuts at: 5527 5539 5885 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 756 397 385 367 346 95
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2445 513
Size: 552 1380 3958
Fragments arranged by size:
3958 1380 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5151
Fragments arranged by size:
5151 683 56
BtsI GCAGTG_nn'
Cuts at: 3054 3406 4200 4220 3054
Size: 352 794 20 4724
Fragments arranged by size:
4724 794 352 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5855
DraI TTT'AAA
Cuts at: 3194 3194
Size: 5890
DraIII CAC_nnn'GTG
Cuts at: 2765 2765
Size: 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2182 4897 809
Size: 507 866 2715 1802
Fragments arranged by size:
2715 1802 866 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3337 4226 5855
Size: 54 1425 50 275 18 1507 889 1629
Cuts at: 5855 8
Size: 43
Fragments arranged by size:
1629 1507 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3337 1537
Size: 275 1525 4090
Fragments arranged by size:
4090 1525 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2929 3029 3820 1353
Size: 1576 100 791 3423
Fragments arranged by size:
3423 1576 791 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4477 4851 4997 387
Size: 1064 138 2888 374 146 1280
Fragments arranged by size:
2888 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2575 3953 4550 5337 5605 1239
Size: 393 943 1378 597 787 268 1524
Fragments arranged by size:
1524 1378 943 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3199 3577 3199
Size: 378 5512
FspI TGC'GCA
Cuts at: 4396 4396
Size: 5890
HaeII r_GCGC'y
Cuts at: 1678 5037 1678
Size: 3359 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 3117 3149 4531
Size: 32 135 32 427 32 1086 32 1382
Cuts at: 4531 4563 4605 4637 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1382 1086 427 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3234 3480 669
Size: 988 281 1296 246 3079
Fragments arranged by size:
3079 1296 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5890
HpaI GTT'AAC
Cuts at: 3480 3480
Size: 5890
KpnI G_GTAC'C
Cuts at: 3215 3215
Size: 5890
MfeI C'AATT_G
Cuts at: 3489 3489
Size: 5890
MluI A'CGCG_T
Cuts at: 5766 5766
Size: 5890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2281 3107 5004 5188 1305
Size: 976 826 1897 184 2007
Fragments arranged by size:
2007 1897 976 826 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4068
Fragments arranged by size:
4068 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3848 4207 4366 518
Size: 648 2682 359 159 2042
Fragments arranged by size:
2682 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2182 3306 3983 5131 5376 1606
Size: 576 1124 677 1148 245 2120
Fragments arranged by size:
2120 1148 1124 677 576 245
NcoI C'CATG_G
Cuts at: 513 1065 2445 513
Size: 552 1380 3958
Fragments arranged by size:
3958 1380 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5890
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5890
NotI GC'GGCC_GC
Cuts at: 3337 3337
Size: 5890
NspI r_CATG'y
Cuts at: 1132 1229 2668 3248 4793 1132
Size: 97 1439 580 1545 2229
Fragments arranged by size:
2229 1545 1439 580 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5890
PciI A'CATG_T
Cuts at: 4789 4789
Size: 5890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2668 3042 1108
Size: 1560 374 3956
Fragments arranged by size:
3956 1560 374
PmeI GTTT'AAAC
Cuts at: 3194 3194
Size: 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 5472 5504 3959
Size: 32 1481 32 4345
Fragments arranged by size:
4345 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5890
PsiI TTA'TAA
Cuts at: 3460 3460
Size: 5890
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2897 2929 2897
Size: 32 5858
PstI C_TGCA'G
Cuts at: 830 3140 3152 3242 830
Size: 2310 12 90 3478
Fragments arranged by size:
3478 2310 90 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5890
PvuII CAG'CTG
Cuts at: 2182 2182
Size: 5890
SalI G'TCGA_C
Cuts at: 1936 3232 1936
Size: 1296 4594
SbfI CC_TGCA'GG
Cuts at: 3242 3242
Size: 5890
ScaI AGT'ACT
Cuts at: 4138 5850 4138
Size: 1712 4178
SfcI C'TryA_G
Cuts at: 826 1045 2400 2899 3136 3148 3238 4373 5054
Size: 219 1355 499 237 12 90 1135 681
Cuts at: 5054 5245 826
Size: 191 1471
Fragments arranged by size:
1471 1355 1135 681 499 237 219 191
90 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5890
SmaI CCC'GGG
Cuts at: 1872 3217 1872
Size: 1345 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2551 3994 4895 5157 820
Size: 197 926 608 1443 901 262 1553
Fragments arranged by size:
1553 1443 926 901 608 262 197
SnaBI TAC'GTA
Cuts at: 493 3681 493
Size: 3188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5890
SphI G_CATG'C
Cuts at: 1132 3248 1132
Size: 2116 3774
SspI AAT'ATT
Cuts at: 5 52 3814 5
Size: 47 3762 2081
Fragments arranged by size:
3762 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5890
StyI C'CwwG_G
Cuts at: 513 1065 2412 2445 2582 3329 513
Size: 552 1347 33 137 747 3074
Fragments arranged by size:
3074 1347 747 552 137 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3917 3934 4087 4272 4717 325
Size: 1255 2337 17 153 185 445 1498
Fragments arranged by size:
2337 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2868 4136 5848
Size: 274 80 33 51 1443 891 1268 1712
Cuts at: 5848 96
Size: 138
Fragments arranged by size:
1712 1443 1268 891 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5850 5882 5850
Size: 32 5858
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5890
XbaI T'CTAG_A
Cuts at: 3226 3226
Size: 5890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2263 2297 2263
Size: 34 5856
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5890
XmaI C'CCGG_G
Cuts at: 1870 3215 1870
Size: 1345 4545
XmnI GAAnn'nnTTC
Cuts at: 4019 4019
Size: 5890
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3698 276
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI