Restriction map for
pFN21AE9513
(Circular) (Six-base) MAPSORT of: pFN21AE9513.seq Check: 5270 from: 1 to: 5800
pFN21AE9513 5800 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2393 3033 2393
Size: 640 5160
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2950 3610 278
Size: 53 83 186 936 1414 660 2468
Fragments arranged by size:
2468 1414 936 660 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2587 2598 3863 5247 5515 1632
Size: 955 11 1265 1384 268 1917
Fragments arranged by size:
1917 1384 1265 955 268 11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 2223 4699 5676 1203
Size: 1020 2476 977 1327
Fragments arranged by size:
2476 1327 1020 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2411 1184
Size: 1227 4573
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2090 2122 2090
Size: 32 5768
AlwNI CAG_nnn'CTG
Cuts at: 2057 2705 3072 3543 5115 2057
Size: 648 367 471 1572 2742
Fragments arranged by size:
2742 1572 648 471 367
ApaLI G'TGCA_C
Cuts at: 2396 2597 3857 5013 2396
Size: 201 1260 1156 3183
Fragments arranged by size:
3183 1260 1156 201
ApoI r'AATT_y
Cuts at: 1493 3109 3335 3487 5596 1493
Size: 1616 226 152 2109 1697
Fragments arranged by size:
2109 1697 1616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 3125 1870
Size: 73 1182 4545
Fragments arranged by size:
4545 1182 73
AvrII C'CTAG_G
Cuts at: 2038 3083 2038
Size: 1045 4755
BamHI G'GATC_C
Cuts at: 2908 3130 3497 2908
Size: 222 367 5211
Fragments arranged by size:
5211 367 222
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3121 4576 618
Size: 325 217 627 1334 1455 1842
Fragments arranged by size:
1842 1455 1334 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3119 1391
Size: 1728 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2709 928
Size: 971 810 4019
Fragments arranged by size:
4019 971 810
BbvCI CC'TCA_GC
Cuts at: 2045 2045
Size: 5800
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2460 4411 136
Size: 107 122 71 631 605 788 1951 1525
Fragments arranged by size:
1951 1525 788 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2322 5795 2322
Size: 3473 2327
BlpI GC'TnA_GC
Cuts at: 3217 3217
Size: 5800
Bme1580I G_kGCm'C
Cuts at: 1790 2400 2434 2511 2601 2640 3861 5017 1790
Size: 610 34 77 90 39 1221 1156 2573
Fragments arranged by size:
2573 1221 1156 610 90 77 39 34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2940 3531 4489
Size: 416 53 83 399 24 1523 591 958
Cuts at: 4489 442
Size: 1753
Fragments arranged by size:
1753 1523 958 591 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4460 1239
Size: 3221 2579
Bpu10I CC'TnA_GC
Cuts at: 2045 2045
Size: 5800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3925 4790 5088 3925
Size: 865 298 4637
Fragments arranged by size:
4637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4463 882
Size: 617 2964 2219
Fragments arranged by size:
2964 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3591 493
Size: 711 2387 2702
Fragments arranged by size:
2702 2387 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2947 3607 3989 4624
Size: 53 83 186 936 1414 660 382 635
Cuts at: 4624 275
Size: 1451
Fragments arranged by size:
1451 1414 936 660 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4233 4631 4905 5052 5463 4233
Size: 398 274 147 411 4570
Fragments arranged by size:
4570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2937 2967 1178
Size: 30 165 30 1534 30 4011
Fragments arranged by size:
4011 1534 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5800
BseYI C'CCAG_C
Cuts at: 2146 2439 2982 5003 5499 2146
Size: 293 543 2021 496 2447
Fragments arranged by size:
2447 2021 543 496 293
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2691 1163
Size: 573 955 4272
Fragments arranged by size:
4272 955 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3250 4011 5039 1540
Size: 275 186 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2400 2601 2919 3861 3946 5017 2400
Size: 201 318 942 85 1071 3183
Fragments arranged by size:
3183 1071 942 318 201 85
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2400 2434 2511 2601 2640 2919 3119
Size: 399 610 34 77 90 39 279 200
Cuts at: 3119 3861 3946 5017 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 610 399 279 200 90
85 77 39 34
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2393 3033 3155 844
Size: 1549 640 122 3489
Fragments arranged by size:
3489 1549 640 122
BsrBI CCG'CTC
Cuts at: 1394 1823 2730 3687 1394
Size: 429 907 957 3507
Fragments arranged by size:
3507 957 907 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4295 4469 65
Size: 1183 3047 174 1396
Fragments arranged by size:
3047 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2495 4444 4631 1700
Size: 110 685 1949 187 2869
Fragments arranged by size:
2869 1949 685 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 2504 3860 4872 2504
Size: 1356 1012 3432
Fragments arranged by size:
3432 1356 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5800
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstXI CCAn_nnnn'nTGG
Cuts at: 2175 2749 2824 2175
Size: 574 75 5151
Fragments arranged by size:
5151 574 75
BstYI r'GATC_y
Cuts at: 1972 2033 2322 2908 3130 3497 3882 3899 5351
Size: 61 289 586 222 367 385 17 1452
Cuts at: 5351 5437 5449 5795 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 586 385 367 346 289 222
86 61 17 12
Bsu36I CC'TnA_GG
Cuts at: 3027 3027
Size: 5800
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 2071 2374 3316 4110 4130 2071
Size: 303 942 794 20 3741
Fragments arranged by size:
3741 942 794 303 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DraIII CAC_nnn'GTG
Cuts at: 2596 2596
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2214 2814
Size: 54 1425 50 275 18 177 207 600
Cuts at: 2814 2886 3051 3247 4136 5765 8
Size: 72 165 196 889 1629 43
Fragments arranged by size:
1629 1425 889 600 275 207 196 177
165 72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3247 1537
Size: 275 1435 4090
Fragments arranged by size:
4090 1435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 2574 3007 3730 1353
Size: 833 388 433 723 3423
Fragments arranged by size:
3423 833 723 433 388
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2180 4387 4761 4907 387
Size: 1064 138 591 2207 374 146 1280
Fragments arranged by size:
2207 1280 1064 591 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2587 2598 3863 4460 5247 5515 1239
Size: 393 955 11 1265 597 787 268 1524
Fragments arranged by size:
1524 1265 955 787 597 393 268 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3026 3026
Size: 5800
EcoO109I rG'GnC_Cy
Cuts at: 2054 2054
Size: 5800
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 4947 1678
Size: 3269 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2937 2969 4441 4473 4515
Size: 32 135 32 1365 32 1472 32 42
Cuts at: 4515 4547 1373
Size: 32 2626
Fragments arranged by size:
2626 1472 1365 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2857 3144 3390 669
Size: 988 281 919 287 246 3079
Fragments arranged by size:
3079 988 919 287 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2221 2805 4914 5098 1305
Size: 916 584 2109 184 2007
Fragments arranged by size:
2109 2007 916 584 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2816 2888 10
Size: 54 1425 343 177 807 72 2922
Fragments arranged by size:
2922 1425 807 343 177 72 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2393 2747 3758 4117 4276 518
Size: 648 1056 171 354 1011 359 159 2042
Fragments arranged by size:
2042 1056 1011 648 359 354 171 159
MspA1I CmG'CkG
Cuts at: 1606 2901 3039 3216 3893 5041 5286 1606
Size: 1295 138 177 677 1148 245 2120
Fragments arranged by size:
2120 1295 1148 677 245 177 138
NaeI GCC'GGC
Cuts at: 2497 2497
Size: 5800
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NgoMIV G'CCGG_C
Cuts at: 2495 2495
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 2227 3158 4703 1132
Size: 97 998 931 1545 2229
Fragments arranged by size:
2229 1545 998 931 97
PasI CC'CwG_GG
Cuts at: 1348 2202 1348
Size: 854 4946
PciI A'CATG_T
Cuts at: 2223 4699 2223
Size: 2476 3324
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2057 1108
Size: 949 4851
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2054 2054
Size: 5800
PshAI GACnn'nnGTC
Cuts at: 1613 2549 1613
Size: 936 4864
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PstI C_TGCA'G
Cuts at: 830 2369 3044 3152 830
Size: 1539 675 108 3478
Fragments arranged by size:
3478 1539 675 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
PvuII CAG'CTG
Cuts at: 2901 3039 2901
Size: 138 5662
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SapI GCTCTTCn'nnn_
Cuts at: 2186 2186
Size: 5800
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 2844 4048 5760 2844
Size: 1204 1712 2884
Fragments arranged by size:
2884 1712 1204
SfcI C'TryA_G
Cuts at: 826 1045 2365 3040 3148 4283 4964 5155 826
Size: 219 1320 675 108 1135 681 191 1471
Fragments arranged by size:
1471 1320 1135 681 675 219 191 108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3904 4805 5067 820
Size: 197 926 1961 901 262 1553
Fragments arranged by size:
1961 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 3013 152
Size: 2861 2939
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 2043 2463 1517
Size: 526 420 4854
Fragments arranged by size:
4854 526 420
StyI C'CwwG_G
Cuts at: 513 1065 2004 2038 3083 3239 513
Size: 552 939 34 1045 156 3074
Fragments arranged by size:
3074 1045 939 552 156 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2436 3827 3844 3997 4182 4627 325
Size: 1255 856 1391 17 153 185 445 1498
Fragments arranged by size:
1498 1391 1255 856 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2012 2842 4046
Size: 274 80 33 51 1443 35 830 1204
Cuts at: 4046 5758 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1204 830 274 138 80 51
35 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5760 5792 5760
Size: 32 5768
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2309 2809 2902 2309
Size: 500 93 5207
Fragments arranged by size:
5207 500 93
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2948 3608 276
Size: 53 83 186 936 1414 660 2468
Fragments arranged by size:
2468 1414 936 660 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI