Restriction map for
pFN21AE9512
(Circular) (Six-base) MAPSORT of: pFN21AE9512.seq Check: 1133 from: 1 to: 6301
pFN21AE9512 6301 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4111 278
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2976 3644 1937
Size: 1039 668 4594
Fragments arranged by size:
4594 1039 668
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3042 3493 4364 5748 6016 1632
Size: 1410 451 871 1384 268 1917
Fragments arranged by size:
1917 1410 1384 871 451 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 5200 6177 1203
Size: 3997 977 1327
Fragments arranged by size:
3997 1327 977
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 2138 1166
Size: 972 5329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2521 1184
Size: 1337 4964
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2795 2827 2795
Size: 32 6269
AlwNI CAG_nnn'CTG
Cuts at: 2760 3104 3334 4044 5616 2760
Size: 344 230 710 1572 3445
Fragments arranged by size:
3445 1572 710 344 230
ApaI G_GGCC'C
Cuts at: 2494 2494
Size: 6301
ApaLI G'TGCA_C
Cuts at: 4358 5514 4358
Size: 1156 5145
ApoI r'AATT_y
Cuts at: 1493 3011 3610 3836 3988 6097 1493
Size: 1518 599 226 152 2109 1697
Fragments arranged by size:
2109 1697 1518 599 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4855 160
Size: 872 3823 1606
Fragments arranged by size:
3823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 2297 3626 1870
Size: 73 354 1329 4545
Fragments arranged by size:
4545 1329 354 73
BamHI G'GATC_C
Cuts at: 3631 3998 3631
Size: 367 5934
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 3622 5077 618
Size: 325 217 627 258 1577 1455 1842
Fragments arranged by size:
1842 1577 1455 627 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2296 2494 2577 2749 3620 1391
Size: 905 198 83 172 871 4072
Fragments arranged by size:
4072 905 871 198 172 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3521 928
Size: 971 1622 3708
Fragments arranged by size:
3708 1622 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4492 4526 1903
Size: 34 2555 34 3678
Fragments arranged by size:
3678 2555 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4193 5403 4193
Size: 1210 5091
BclI T'GATC_A
Cuts at: 1548 2130 2373 1548
Size: 582 243 5476
Fragments arranged by size:
5476 582 243
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2885 4912 136
Size: 107 122 71 631 605 1213 2027 1525
Fragments arranged by size:
2027 1525 1213 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2230 6296 2230
Size: 4066 2235
BlpI GC'TnA_GC
Cuts at: 2213 2218 2895 3718 2213
Size: 5 677 823 4796
Fragments arranged by size:
4796 823 677 5
Bme1580I G_kGCm'C
Cuts at: 1790 2494 4362 5518 1790
Size: 704 1868 1156 2573
Fragments arranged by size:
2573 1868 1156 704
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2045 2856 3221
Size: 416 53 83 399 24 628 811 365
Cuts at: 3221 3416 4032 4990 442
Size: 195 616 958 1753
Fragments arranged by size:
1753 958 811 628 616 416 399 365
195 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2678 3081 4961 1239
Size: 1439 403 1880 2579
Fragments arranged by size:
2579 1880 1439 403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2171 4426 5291 5589 2171
Size: 2255 865 298 2883
Fragments arranged by size:
2883 2255 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3173 4964 882
Size: 617 1674 1791 2219
Fragments arranged by size:
2219 1791 1674 617
BsaAI yAC'GTr
Cuts at: 493 1204 2338 4092 493
Size: 711 1134 1754 2702
Fragments arranged by size:
2702 1754 1134 711
BsaBI GATnn'nnATC
Cuts at: 6295 6295
Size: 6301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4108 4490 5125 275
Size: 53 83 186 936 2575 382 635 1451
Fragments arranged by size:
2575 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4734 5132 5406 5553 5964 4734
Size: 398 274 147 411 5071
Fragments arranged by size:
5071 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2795 2825 1178
Size: 30 165 30 1392 30 4654
Fragments arranged by size:
4654 1392 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6301
BseYI C'CCAG_C
Cuts at: 2289 2494 2790 3269 5504 6000 2289
Size: 205 296 479 2235 496 2590
Fragments arranged by size:
2590 2235 496 479 296 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2247 1163
Size: 573 511 5217
Fragments arranged by size:
5217 573 511
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3751 4512 5540 1540
Size: 275 186 1750 761 1028 2301
Fragments arranged by size:
2301 1750 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2837 4362 4447 5518 2837
Size: 1525 85 1071 3620
Fragments arranged by size:
3620 1525 1071 85
BsmI GAATG_Cn'
Cuts at: 3812 3905 3812
Size: 93 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2296 2494 2577 2749 2837 3620 4362
Size: 399 506 198 83 172 88 783 742
Cuts at: 4362 4447 5518 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 506 399 198 172
88 85 83
BspEI T'CCGG_A
Cuts at: 5964 5964
Size: 6301
BspHI T'CATG_A
Cuts at: 2157 2358 2451 2628 4190 5920 2157
Size: 201 93 177 1562 1730 2538
Fragments arranged by size:
2538 1730 1562 201 177 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 3656 844
Size: 1373 1439 3489
Fragments arranged by size:
3489 1439 1373
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2100 2152 2598 4796 4970 65
Size: 1183 852 52 446 2198 174 1396
Fragments arranged by size:
2198 1396 1183 852 446 174 52
BsrFI r'CCGG_y
Cuts at: 1700 1810 4945 5132 1700
Size: 110 3135 187 2869
Fragments arranged by size:
3135 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6301
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 4361 5373 4361
Size: 1012 5289
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6301
BstBI TT'CG_AA
Cuts at: 5105 5105
Size: 6301
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6301
BstXI CCAn_nnnn'nTGG
Cuts at: 2683 2867 3349 2683
Size: 184 482 5635
Fragments arranged by size:
5635 482 184
BstYI r'GATC_y
Cuts at: 1972 2230 2556 2756 3631 3998 4383 4400 5852
Size: 258 326 200 875 367 385 17 1452
Cuts at: 5852 5938 5950 6296 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 875 385 367 346 326 258
200 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4810
Fragments arranged by size:
4810 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5562
Fragments arranged by size:
5562 683 56
BtsI GCAGTG_nn'
Cuts at: 2592 3817 4611 4631 2592
Size: 1225 794 20 4262
Fragments arranged by size:
4262 1225 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6266
DraI TTT'AAA
Cuts at: 3605 3605
Size: 6301
DraIII CAC_nnn'GTG
Cuts at: 3202 3338 3432 3202
Size: 136 94 6071
Fragments arranged by size:
6071 136 94
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3120 3416 5308 809
Size: 507 1804 296 1892 1802
Fragments arranged by size:
1892 1804 1802 507 296
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2340 3231 3748
Size: 54 1425 50 275 18 510 891 517
Cuts at: 3748 4637 6266 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 891 889 517 510 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3748 1537
Size: 275 1936 4090
Fragments arranged by size:
4090 1936 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2720 3018 3558 4231 1353
Size: 1367 298 540 673 3423
Fragments arranged by size:
3423 1367 673 540 298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4888 5262 5408 387
Size: 1064 138 3299 374 146 1280
Fragments arranged by size:
3299 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2678 3042 3081 3493 4364 4961 5748
Size: 393 1046 364 39 412 871 597 787
Cuts at: 5748 6016 1239
Size: 268 1524
Fragments arranged by size:
1524 1046 871 787 597 412 393 364
268 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2827 2827
Size: 6301
EcoO109I rG'GnC_Cy
Cuts at: 2491 2680 2491
Size: 189 6112
EcoRI G'AATT_C
Cuts at: 3011 3610 3988 3011
Size: 599 378 5324
Fragments arranged by size:
5324 599 378
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 2880 5448 1678
Size: 1202 2568 2531
Fragments arranged by size:
2568 2531 1202
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4942 4974 5016 5048 1373
Size: 32 135 32 3370 32 42 32 2626
Fragments arranged by size:
3370 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2176 2197 3475 3645 3891 669
Size: 988 281 238 21 1278 170 246 3079
Fragments arranged by size:
3079 1278 988 281 246 238 170 21
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6301
HpaI GTT'AAC
Cuts at: 2197 3891 2197
Size: 1694 4607
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 2881 3900 2881
Size: 1019 5282
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5415 5599 1305
Size: 4110 184 2007
Fragments arranged by size:
4110 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2342 3233 10
Size: 54 1425 343 510 891 3078
Fragments arranged by size:
3078 1425 891 510 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 2279 4259 4618 4777 518
Size: 648 972 141 1980 359 159 2042
Fragments arranged by size:
2042 1980 972 648 359 159 141
MspA1I CmG'CkG
Cuts at: 1606 2108 2794 3104 3717 4394 5542 5787 1606
Size: 502 686 310 613 677 1148 245 2120
Fragments arranged by size:
2120 1148 686 677 613 502 310 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4810
Fragments arranged by size:
4810 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6301
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 3659 5204 1132
Size: 97 2430 1545 2229
Fragments arranged by size:
2430 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3002 1348
Size: 1654 4647
PciI A'CATG_T
Cuts at: 5200 5200
Size: 6301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2068 1108
Size: 960 5341
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2795 2827 4370 4402 5883 5915 2795
Size: 32 1543 32 1481 32 3181
Fragments arranged by size:
3181 1543 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2680 2680
Size: 6301
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6301
PsiI TTA'TAA
Cuts at: 3871 3871
Size: 6301
PspOMI G'GGCC_C
Cuts at: 2490 2490
Size: 6301
PstI C_TGCA'G
Cuts at: 830 3653 830
Size: 2823 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
PvuII CAG'CTG
Cuts at: 2108 3104 2108
Size: 996 5305
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 2516 4549 6261 2516
Size: 2033 1712 2556
Fragments arranged by size:
2556 2033 1712
SfcI C'TryA_G
Cuts at: 826 1045 2911 3649 4784 5465 5656 826
Size: 219 1866 738 1135 681 191 1471
Fragments arranged by size:
1866 1471 1135 738 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3628 1872
Size: 1756 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 4405 5306 5568 820
Size: 197 926 243 2219 901 262 1553
Fragments arranged by size:
2219 1553 926 901 262 243 197
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SspI AAT'ATT
Cuts at: 5 52 4225 5
Size: 47 4173 2081
Fragments arranged by size:
4173 2081 47
StuI AGG'CCT
Cuts at: 1517 2810 3255 1517
Size: 1293 445 4563
Fragments arranged by size:
4563 1293 445
StyI C'CwwG_G
Cuts at: 513 1065 2004 2676 3234 3740 513
Size: 552 939 672 558 506 3074
Fragments arranged by size:
3074 939 672 558 552 506
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4328 4345 4498 4683 5128 325
Size: 1255 2748 17 153 185 445 1498
Fragments arranged by size:
2748 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2514 3573 4547
Size: 274 80 33 51 1443 537 1059 974
Cuts at: 4547 6259 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1059 974 537 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6261 6293 6261
Size: 32 6269
Tth111I GACn'n_nGTC
Cuts at: 1316 2960 1316
Size: 1644 4657
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2880 3429 2880
Size: 549 5752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 3626 1870
Size: 1756 4545
XmnI GAAnn'nnTTC
Cuts at: 4430 4430
Size: 6301
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4109 276
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI