Restriction map for
pFN21AE9510
(Circular) (Six-base) MAPSORT of: pFN21AE9510.seq Check: 9483 from: 1 to: 6142
pFN21AE9510 6142 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3411 3411
Size: 6142
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3485 1937
Size: 1548 4594
Acc65I G'GTAC_C
Cuts at: 3463 3463
Size: 6142
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2203 4205 5589 5857 1632
Size: 571 2002 1384 268 1917
Fragments arranged by size:
2002 1917 1384 571 268
AfeI AGC'GCT
Cuts at: 2841 2841
Size: 6142
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 5041 6018 1203
Size: 3838 977 1327
Fragments arranged by size:
3838 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3173 1184
Size: 1989 4153
AlwNI CAG_nnn'CTG
Cuts at: 2572 3885 5457 2572
Size: 1313 1572 3257
Fragments arranged by size:
3257 1572 1313
ApaI G_GGCC'C
Cuts at: 2705 3001 2705
Size: 296 5846
ApaLI G'TGCA_C
Cuts at: 4199 5355 4199
Size: 1156 4986
ApoI r'AATT_y
Cuts at: 1493 3451 3677 3829 5938 1493
Size: 1958 226 152 2109 1697
Fragments arranged by size:
2109 1958 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 2696 2780 2793 3183 3467 1870
Size: 73 92 661 84 13 390 284 4545
Fragments arranged by size:
4545 661 390 284 92 84 73 13
BamHI G'GATC_C
Cuts at: 3472 3839 3472
Size: 367 5775
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2306 2497 2542 3399
Size: 325 217 627 462 57 191 45 857
Cuts at: 3399 3463 4918 618
Size: 64 1455 1842
Fragments arranged by size:
1842 1455 857 627 462 325 217 191
64 57 45
BanII G_rGCy'C
Cuts at: 1391 2705 2747 3001 3461 1391
Size: 1314 42 254 460 4072
Fragments arranged by size:
4072 1314 460 254 42
BbeI G_GCGC'C
Cuts at: 2253 2310 2501 2253
Size: 57 191 5894
Fragments arranged by size:
5894 191 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2095 928
Size: 971 196 4975
Fragments arranged by size:
4975 971 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4333 4367 1903
Size: 34 2396 34 3678
Fragments arranged by size:
3678 2396 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3095 3122 4753
Size: 107 122 71 631 605 1423 27 1631
Cuts at: 4753 136
Size: 1525
Fragments arranged by size:
1631 1525 1423 631 605 122 107 71
27
BglII A'GATC_T
Cuts at: 6137 6137
Size: 6142
BlpI GC'TnA_GC
Cuts at: 3559 3559
Size: 6142
Bme1580I G_kGCm'C
Cuts at: 1790 2547 2705 3001 4203 5359 1790
Size: 757 158 296 1202 1156 2573
Fragments arranged by size:
2573 1202 1156 757 296 158
BmgBI CAC'GTC
Cuts at: 1318 2064 1318
Size: 746 5396
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3873 4831 442
Size: 416 53 83 399 24 2456 958 1753
Fragments arranged by size:
2456 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3227 1056
Size: 2171 3971
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2911 2911
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2840 2998 4802 1239
Size: 1601 158 1804 2579
Fragments arranged by size:
2579 1804 1601 158
Bpu10I CC'TnA_GC
Cuts at: 2129 2896 2129
Size: 767 5375
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2723 4267 5132 5430 2723
Size: 1544 865 298 3435
Fragments arranged by size:
3435 1544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4805 882
Size: 617 3306 2219
Fragments arranged by size:
3306 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2344 3933 493
Size: 711 1140 1589 2702
Fragments arranged by size:
2702 1589 1140 711
BsaBI GATnn'nnATC
Cuts at: 6136 6136
Size: 6142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2250 2307 2498 3245
Size: 53 83 186 936 717 57 191 747
Cuts at: 3245 3949 4331 4966 275
Size: 704 382 635 1451
Fragments arranged by size:
1451 936 747 717 704 635 382 191
186 83 57 53
BsaWI w'CCGG_w
Cuts at: 4575 4973 5247 5394 5805 4575
Size: 398 274 147 411 4912
Fragments arranged by size:
4912 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2996 3026 1178
Size: 30 165 30 1593 30 4294
Fragments arranged by size:
4294 1593 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2813 2837 1186
Size: 1627 24 4491
Fragments arranged by size:
4491 1627 24
BseYI C'CCAG_C
Cuts at: 2581 2929 3007 5345 5841 2581
Size: 348 78 2338 496 2882
Fragments arranged by size:
2882 2338 496 348 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5569
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 4353 5381 1540
Size: 275 186 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2493 4203 4288 5359 2493
Size: 1710 85 1071 3276
Fragments arranged by size:
3276 1710 1071 85
BsmI GAATG_Cn'
Cuts at: 3653 3746 3653
Size: 93 6049
BsmBI CGTCTCn'nnnn_
Cuts at: 2081 2081
Size: 6142
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2493 2547 2705 2747 3001 3461 4203
Size: 399 703 54 158 42 254 460 742
Cuts at: 4203 4288 5359 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 703 460 399 254 158
85 54 42
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 2364 2457 2634 4031 5761 2364
Size: 93 177 1397 1730 2745
Fragments arranged by size:
2745 1730 1397 177 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2628 3411 3497 844
Size: 1784 783 86 3489
Fragments arranged by size:
3489 1784 783 86
BsrBI CCG'CTC
Cuts at: 1394 1823 4029 1394
Size: 429 2206 3507
Fragments arranged by size:
3507 2206 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2106 2604 2815 3061 4637 4811 65
Size: 1183 858 498 211 246 1576 174 1396
Fragments arranged by size:
1576 1396 1183 858 498 246 211 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2691 3094 4786 4973 1700
Size: 110 881 403 1692 187 2869
Fragments arranged by size:
2869 1692 881 403 187 110
BsrGI T'GTAC_A
Cuts at: 96 2277 2646 96
Size: 2181 369 3592
Fragments arranged by size:
3592 2181 369
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3406 794
Size: 2612 3530
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstXI CCAn_nnnn'nTGG
Cuts at: 2446 2728 2446
Size: 282 5860
BstYI r'GATC_y
Cuts at: 1972 3027 3472 3839 4224 4241 5693 5779 5791
Size: 1055 445 367 385 17 1452 86 12
Cuts at: 5791 6137 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1055 445 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2310 2862 2993 513
Size: 552 939 306 552 131 3662
Fragments arranged by size:
3662 939 552 552 306 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3127 487
Size: 683 56 1901 3502
Fragments arranged by size:
3502 1901 683 56
BtsI GCAGTG_nn'
Cuts at: 2592 2908 3432 3658 4452 4472 2592
Size: 316 524 226 794 20 4262
Fragments arranged by size:
4262 794 524 316 226 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3385 3446 3385
Size: 61 6081
DraIII CAC_nnn'GTG
Cuts at: 3306 3306
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5149 809
Size: 507 3833 1802
Fragments arranged by size:
3833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2664 3114
Size: 54 1425 50 275 18 177 657 450
Cuts at: 3114 3589 4478 6107 8
Size: 475 889 1629 43
Fragments arranged by size:
1629 1425 889 657 475 450 275 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 1537
Size: 275 1777 4090
Fragments arranged by size:
4090 1777 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 4072 1353
Size: 869 1850 3423
Fragments arranged by size:
3423 1850 869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4729 5103 5249 387
Size: 1064 138 3140 374 146 1280
Fragments arranged by size:
3140 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2203 2840 2998 4205 4802 5589 5857
Size: 393 571 637 158 1207 597 787 268
Cuts at: 5857 1239
Size: 1524
Fragments arranged by size:
1524 1207 787 637 597 571 393 268
158
EcoO109I rG'GnC_Cy
Cuts at: 2701 2827 2998 2701
Size: 126 171 5845
Fragments arranged by size:
5845 171 126
EcoRI G'AATT_C
Cuts at: 3451 3829 3451
Size: 378 5764
FspI TGC'GCA
Cuts at: 2807 3161 4648 2807
Size: 354 1487 4301
Fragments arranged by size:
4301 1487 354
HaeII r_GCGC'y
Cuts at: 1678 2253 2310 2501 2843 5289 1678
Size: 575 57 191 342 2446 2531
Fragments arranged by size:
2531 2446 575 342 191 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4783 4815 4857 4889 1373
Size: 32 135 32 3211 32 42 32 2626
Fragments arranged by size:
3211 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2182 2203 3337 3486 3732 669
Size: 988 281 244 21 1134 149 246 3079
Fragments arranged by size:
3079 1134 988 281 246 244 149 21
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KasI G'GCGC_C
Cuts at: 2249 2306 2497 2249
Size: 57 191 5894
Fragments arranged by size:
5894 191 57
KpnI G_GTAC'C
Cuts at: 3467 3467
Size: 6142
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2601 5256 5440 1305
Size: 1296 2655 184 2007
Fragments arranged by size:
2655 2007 1296 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2666 10
Size: 54 1425 343 177 657 3486
Fragments arranged by size:
3486 1425 657 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 2444 2639 4100 4459 4618 518
Size: 648 993 285 195 1461 359 159 2042
Fragments arranged by size:
2042 1461 993 648 359 285 195 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2995 3558 4235 5383 5628 1606
Size: 585 804 563 677 1148 245 2120
Fragments arranged by size:
2120 1148 804 677 585 563 245
NaeI GCC'GGC
Cuts at: 3096 3096
Size: 6142
NarI GG'CG_CC
Cuts at: 2250 2307 2498 2250
Size: 57 191 5894
Fragments arranged by size:
5894 191 57
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4651
Fragments arranged by size:
4651 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NgoMIV G'CCGG_C
Cuts at: 3094 3094
Size: 6142
NheI G'CTAG_C
Cuts at: 1052 3223 1052
Size: 2171 3971
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 3106 3500 5045 1132
Size: 97 1877 394 1545 2229
Fragments arranged by size:
2229 1877 1545 394 97
PasI CC'CwG_GG
Cuts at: 1348 2302 1348
Size: 954 5188
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6142
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PspOMI G'GGCC_C
Cuts at: 2701 2997 2701
Size: 296 5846
PstI C_TGCA'G
Cuts at: 830 2196 2991 3494 830
Size: 1366 795 503 3478
Fragments arranged by size:
3478 1366 795 503
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
PvuII CAG'CTG
Cuts at: 2191 2191
Size: 6142
SacII CC_GC'GG
Cuts at: 2996 2996
Size: 6142
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 4390 6102 4390
Size: 1712 4430
SexAI A'CCwGG_T
Cuts at: 3153 3153
Size: 6142
SfcI C'TryA_G
Cuts at: 826 1045 2192 2987 3490 4625 5306 5497 826
Size: 219 1147 795 503 1135 681 191 1471
Fragments arranged by size:
1471 1147 1135 795 681 503 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3122 3122
Size: 6142
SfoI GGC'GCC
Cuts at: 2251 2308 2499 2251
Size: 57 191 5894
Fragments arranged by size:
5894 191 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 2782 2795 3185 3469 1872
Size: 910 13 390 284 4545
Fragments arranged by size:
4545 910 390 284 13
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2738 4246 5147 5409 820
Size: 197 926 795 1508 901 262 1553
Fragments arranged by size:
1553 1508 926 901 795 262 197
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 3106 3500 1132
Size: 1974 394 3774
Fragments arranged by size:
3774 1974 394
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 2454 1517
Size: 937 5205
StyI C'CwwG_G
Cuts at: 513 1065 2004 2919 3581 513
Size: 552 939 915 662 3074
Fragments arranged by size:
3074 939 915 662 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2924 4169 4186 4339 4524 4969 325
Size: 1255 1344 1245 17 153 185 445 1498
Fragments arranged by size:
1498 1344 1255 1245 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 2646 4388
Size: 274 80 33 51 1443 300 369 1742
Cuts at: 4388 6100 96
Size: 1712 138
Fragments arranged by size:
1742 1712 1443 369 300 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6102 6134 6102
Size: 32 6110
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3015 3015
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 2780 2793 3183 3467 1870
Size: 910 13 390 284 4545
Fragments arranged by size:
4545 910 390 284 13
XmnI GAAnn'nnTTC
Cuts at: 3275 4271 3275
Size: 996 5146
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SanDI SapI SrfI SwaI