(Circular) (Six-base) MAPSORT of: pFN21AE9508.seq Check: 501 from: 1 to: 5671 pFN21AE9508 5671 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3481 278 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3014 1937 Size: 1077 4594 Acc65I G'GTAC_C Cuts at: 2391 2992 2391 Size: 601 5070 AclI AA'CG_TT Cuts at: 1563 3798 4171 1563 Size: 2235 373 3063 Fragments arranged by size: 3063 2235 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2885 3734 5118 5386 1632 Size: 1253 849 1384 268 1917 Fragments arranged by size: 1917 1384 1253 849 268 AfeI AGC'GCT Cuts at: 2363 2363 Size: 5671 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5474 AflIII A'CryG_T Cuts at: 1203 4570 5547 1203 Size: 3367 977 1327 Fragments arranged by size: 3367 1327 977 AgeI A'CCGG_T Cuts at: 4502 4502 Size: 5671 AhdI GACnn_n'nnGTC Cuts at: 4400 4400 Size: 5671 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5671 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5671 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2171 2203 2465 2497 2171 Size: 32 262 32 5345 Fragments arranged by size: 5345 262 32 32 AlwNI CAG_nnn'CTG Cuts at: 2086 2863 3414 4986 2086 Size: 777 551 1572 2771 Fragments arranged by size: 2771 1572 777 551 ApaI G_GGCC'C Cuts at: 2716 2716 Size: 5671 ApaLI G'TGCA_C Cuts at: 3728 4884 3728 Size: 1156 4515 ApoI r'AATT_y Cuts at: 1493 2737 2980 3206 3358 5467 1493 Size: 1244 243 226 152 2109 1697 Fragments arranged by size: 2109 1697 1244 243 226 152 AseI AT'TA_AT Cuts at: 160 1032 4225 160 Size: 872 3193 1606 Fragments arranged by size: 3193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 AvaI C'yCGr_G Cuts at: 1870 1943 2996 1870 Size: 73 1053 4545 Fragments arranged by size: 4545 1053 73 BamHI G'GATC_C Cuts at: 2008 2607 2618 3001 3368 2008 Size: 599 11 383 367 4311 Fragments arranged by size: 4311 599 383 367 11 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2391 2992 4447 618 Size: 325 217 627 604 601 1455 1842 Fragments arranged by size: 1842 1455 627 604 601 325 217 BanII G_rGCy'C Cuts at: 1391 2716 2834 2887 2990 1391 Size: 1325 118 53 103 4072 Fragments arranged by size: 4072 1325 118 103 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2017 2668 928 Size: 971 118 651 3931 Fragments arranged by size: 3931 971 651 118 BbvCI CC'TCA_GC Cuts at: 2231 2231 Size: 5671 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2278 2312 3862 3896 1903 Size: 34 341 34 1550 34 3678 Fragments arranged by size: 3678 1550 341 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3563 4773 3563 Size: 1210 4461 BclI T'GATC_A Cuts at: 1548 1548 Size: 5671 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4282 136 Size: 107 122 71 631 605 2610 1525 Fragments arranged by size: 2610 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5666 5666 Size: 5671 BlpI GC'TnA_GC Cuts at: 2342 3088 2342 Size: 746 4925 Bme1580I G_kGCm'C Cuts at: 1790 2716 3732 4888 1790 Size: 926 1016 1156 2573 Fragments arranged by size: 2573 1156 1016 926 BmgBI CAC'GTC Cuts at: 1318 2057 2106 1318 Size: 739 49 4883 Fragments arranged by size: 4883 739 49 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2015 2537 3402 Size: 416 53 83 399 24 598 522 865 Cuts at: 3402 4360 442 Size: 958 1753 Fragments arranged by size: 1753 958 865 598 522 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2492 2861 2963 4331 1239 Size: 1253 369 102 1368 2579 Fragments arranged by size: 2579 1368 1253 369 102 Bpu10I CC'TnA_GC Cuts at: 2087 2231 2087 Size: 144 5527 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3796 4661 4959 3796 Size: 865 298 4508 Fragments arranged by size: 4508 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4334 882 Size: 617 2835 2219 Fragments arranged by size: 2835 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2650 3462 493 Size: 711 1446 812 2702 Fragments arranged by size: 2702 1446 812 711 BsaBI GATnn'nnATC Cuts at: 5665 5665 Size: 5671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3478 3860 4495 275 Size: 53 83 186 936 1945 382 635 1451 Fragments arranged by size: 1945 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4104 4502 4776 4923 5334 4104 Size: 398 274 147 411 4441 Fragments arranged by size: 4441 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2171 2201 2465 2470 2495 Size: 30 165 30 768 30 264 5 25 Cuts at: 2495 2500 1178 Size: 5 4349 Fragments arranged by size: 4349 768 264 165 30 30 30 25 5 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2672 1186 Size: 889 597 4185 Fragments arranged by size: 4185 889 597 BseYI C'CCAG_C Cuts at: 4874 5370 4874 Size: 496 5175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5098 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2427 3121 3882 4910 1540 Size: 275 186 426 694 761 1028 2301 Fragments arranged by size: 2301 1028 761 694 426 275 186 BsiHKAI G_wGCw'C Cuts at: 2151 3732 3817 4888 2151 Size: 1581 85 1071 2934 Fragments arranged by size: 2934 1581 1071 85 BsmI GAATG_Cn' Cuts at: 2703 3182 3275 2703 Size: 479 93 5099 Fragments arranged by size: 5099 479 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2716 2834 2887 2990 3732 3817 Size: 399 361 565 118 53 103 742 85 Cuts at: 3817 4888 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 565 399 361 118 103 85 53 BspEI T'CCGG_A Cuts at: 5334 5334 Size: 5671 BspHI T'CATG_A Cuts at: 3560 5290 3560 Size: 1730 3941 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3026 844 Size: 2182 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3558 1394 Size: 429 1735 3507 Fragments arranged by size: 3507 1735 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4166 4340 65 Size: 1183 2918 174 1396 Fragments arranged by size: 2918 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4315 4502 1700 Size: 110 2505 187 2869 Fragments arranged by size: 2869 2505 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5671 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5671 BssSI C'ACGA_G Cuts at: 3731 4743 3731 Size: 1012 4659 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5671 BstBI TT'CG_AA Cuts at: 4475 4475 Size: 5671 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5671 BstXI CCAn_nnnn'nTGG Cuts at: 2550 2550 Size: 5671 BstYI r'GATC_y Cuts at: 1972 2008 2607 2618 3001 3368 3753 3770 5222 Size: 36 599 11 383 367 385 17 1452 Cuts at: 5222 5308 5320 5666 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 599 385 383 367 346 86 36 17 12 11 Bsu36I CC'TnA_GG Cuts at: 2901 2931 2901 Size: 30 5641 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4180 Fragments arranged by size: 4180 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4932 Fragments arranged by size: 4932 683 56 BtsI GCAGTG_nn' Cuts at: 3187 3981 4001 3187 Size: 794 20 4857 Fragments arranged by size: 4857 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5636 DraI TTT'AAA Cuts at: 2975 2975 Size: 5671 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4678 809 Size: 507 3362 1802 Fragments arranged by size: 3362 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 3118 4007 Size: 54 1425 50 275 18 378 910 889 Cuts at: 4007 5636 8 Size: 1629 43 Fragments arranged by size: 1629 1425 910 889 378 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3118 1537 Size: 275 1306 4090 Fragments arranged by size: 4090 1306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2231 3601 1353 Size: 878 1370 3423 Fragments arranged by size: 3423 1370 878 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4258 4632 4778 387 Size: 1064 138 2669 374 146 1280 Fragments arranged by size: 2669 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2492 2861 2885 2963 3734 4331 5118 Size: 393 860 369 24 78 771 597 787 Cuts at: 5118 5386 1239 Size: 268 1524 Fragments arranged by size: 1524 860 787 771 597 393 369 268 78 24 EcoO109I rG'GnC_Cy Cuts at: 2446 2712 2713 2446 Size: 266 1 5404 Fragments arranged by size: 5404 266 1 EcoRI G'AATT_C Cuts at: 2980 3358 2980 Size: 378 5293 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2942 2942 Size: 5671 FspI TGC'GCA Cuts at: 2174 4177 2174 Size: 2003 3668 HaeII r_GCGC'y Cuts at: 1678 2349 2365 4818 1678 Size: 671 16 2453 2531 Fragments arranged by size: 2531 2453 671 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4312 4344 4386 4418 1373 Size: 32 135 32 2740 32 42 32 2626 Fragments arranged by size: 2740 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3015 3261 669 Size: 988 281 1077 246 3079 Fragments arranged by size: 3079 1077 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5671 HpaI GTT'AAC Cuts at: 3261 3261 Size: 5671 KpnI G_GTAC'C Cuts at: 2395 2996 2395 Size: 601 5070 MfeI C'AATT_G Cuts at: 3270 3270 Size: 5671 MluI A'CGCG_T Cuts at: 5547 5547 Size: 5671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2036 2104 4785 4969 1305 Size: 731 68 2681 184 2007 Fragments arranged by size: 2681 2007 731 184 68 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3849 Fragments arranged by size: 3849 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3629 3988 4147 518 Size: 648 2463 359 159 2042 Fragments arranged by size: 2463 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2397 2423 2635 3087 3764 4912 5157 1606 Size: 791 26 212 452 677 1148 245 2120 Fragments arranged by size: 2120 1148 791 677 452 245 212 26 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4180 Fragments arranged by size: 4180 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5671 NotI GC'GGCC_GC Cuts at: 3118 3118 Size: 5671 NspI r_CATG'y Cuts at: 1132 1229 2173 2779 3029 4574 1132 Size: 97 944 606 250 1545 2229 Fragments arranged by size: 2229 1545 944 606 250 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5671 PciI A'CATG_T Cuts at: 4570 4570 Size: 5671 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5671 PfoI T'CCnGG_A Cuts at: 2621 2621 Size: 5671 PmeI GTTT'AAAC Cuts at: 2975 2975 Size: 5671 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2171 2203 2465 2497 3740 3772 5253 5285 2171 Size: 32 262 32 1243 32 1481 32 2557 Fragments arranged by size: 2557 1481 1243 262 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5671 PsiI TTA'TAA Cuts at: 3241 3241 Size: 5671 PspOMI G'GGCC_C Cuts at: 2712 2712 Size: 5671 PstI C_TGCA'G Cuts at: 830 2892 3023 830 Size: 2062 131 3478 Fragments arranged by size: 3478 2062 131 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5671 SalI G'TCGA_C Cuts at: 1936 3013 1936 Size: 1077 4594 SbfI CC_TGCA'GG Cuts at: 3023 3023 Size: 5671 ScaI AGT'ACT Cuts at: 3919 5631 3919 Size: 1712 3959 SfcI C'TryA_G Cuts at: 826 1045 2888 3019 4154 4835 5026 826 Size: 219 1843 131 1135 681 191 1471 Fragments arranged by size: 1843 1471 1135 681 219 191 131 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5671 SmaI CCC'GGG Cuts at: 1872 2998 1872 Size: 1126 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3775 4676 4938 820 Size: 197 926 1832 901 262 1553 Fragments arranged by size: 1832 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3462 493 Size: 2969 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5671 SphI G_CATG'C Cuts at: 1132 2173 3029 1132 Size: 1041 856 3774 Fragments arranged by size: 3774 1041 856 SspI AAT'ATT Cuts at: 5 52 3595 5 Size: 47 3543 2081 Fragments arranged by size: 3543 2081 47 StuI AGG'CCT Cuts at: 1517 2331 1517 Size: 814 4857 StyI C'CwwG_G Cuts at: 513 1065 2004 3110 513 Size: 552 939 1106 3074 Fragments arranged by size: 3074 1106 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3698 3715 3868 4053 4498 325 Size: 1255 2118 17 153 185 445 1498 Fragments arranged by size: 2118 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3917 5629 96 Size: 274 80 33 51 1443 1940 1712 138 Fragments arranged by size: 1940 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2470 2502 2599 2631 5631 5663 2470 Size: 32 97 32 3000 32 2478 Fragments arranged by size: 3000 2478 97 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5671 XbaI T'CTAG_A Cuts at: 3007 3007 Size: 5671 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5671 XmaI C'CCGG_G Cuts at: 1870 2996 1870 Size: 1126 4545 XmnI GAAnn'nnTTC Cuts at: 3800 3800 Size: 5671 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3479 276 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI