Restriction map for
pFN21AE9508
(Circular) (Six-base) MAPSORT of: pFN21AE9508.seq Check: 501 from: 1 to: 5671
pFN21AE9508 5671 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2391 2992 2391
Size: 601 5070
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2885 3734 5118 5386 1632
Size: 1253 849 1384 268 1917
Fragments arranged by size:
1917 1384 1253 849 268
AfeI AGC'GCT
Cuts at: 2363 2363
Size: 5671
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 4570 5547 1203
Size: 3367 977 1327
Fragments arranged by size:
3367 1327 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2171 2203 2465 2497 2171
Size: 32 262 32 5345
Fragments arranged by size:
5345 262 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2086 2863 3414 4986 2086
Size: 777 551 1572 2771
Fragments arranged by size:
2771 1572 777 551
ApaI G_GGCC'C
Cuts at: 2716 2716
Size: 5671
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2737 2980 3206 3358 5467 1493
Size: 1244 243 226 152 2109 1697
Fragments arranged by size:
2109 1697 1244 243 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2996 1870
Size: 73 1053 4545
Fragments arranged by size:
4545 1053 73
BamHI G'GATC_C
Cuts at: 2008 2607 2618 3001 3368 2008
Size: 599 11 383 367 4311
Fragments arranged by size:
4311 599 383 367 11
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2391 2992 4447 618
Size: 325 217 627 604 601 1455 1842
Fragments arranged by size:
1842 1455 627 604 601 325 217
BanII G_rGCy'C
Cuts at: 1391 2716 2834 2887 2990 1391
Size: 1325 118 53 103 4072
Fragments arranged by size:
4072 1325 118 103 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2017 2668 928
Size: 971 118 651 3931
Fragments arranged by size:
3931 971 651 118
BbvCI CC'TCA_GC
Cuts at: 2231 2231
Size: 5671
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2278 2312 3862 3896 1903
Size: 34 341 34 1550 34 3678
Fragments arranged by size:
3678 1550 341 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4282 136
Size: 107 122 71 631 605 2610 1525
Fragments arranged by size:
2610 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 2342 3088 2342
Size: 746 4925
Bme1580I G_kGCm'C
Cuts at: 1790 2716 3732 4888 1790
Size: 926 1016 1156 2573
Fragments arranged by size:
2573 1156 1016 926
BmgBI CAC'GTC
Cuts at: 1318 2057 2106 1318
Size: 739 49 4883
Fragments arranged by size:
4883 739 49
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2015 2537 3402
Size: 416 53 83 399 24 598 522 865
Cuts at: 3402 4360 442
Size: 958 1753
Fragments arranged by size:
1753 958 865 598 522 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2492 2861 2963 4331 1239
Size: 1253 369 102 1368 2579
Fragments arranged by size:
2579 1368 1253 369 102
Bpu10I CC'TnA_GC
Cuts at: 2087 2231 2087
Size: 144 5527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3796 4661 4959 3796
Size: 865 298 4508
Fragments arranged by size:
4508 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4334 882
Size: 617 2835 2219
Fragments arranged by size:
2835 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2650 3462 493
Size: 711 1446 812 2702
Fragments arranged by size:
2702 1446 812 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2171 2201 2465 2470 2495
Size: 30 165 30 768 30 264 5 25
Cuts at: 2495 2500 1178
Size: 5 4349
Fragments arranged by size:
4349 768 264 165 30 30 30 25
5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2672 1186
Size: 889 597 4185
Fragments arranged by size:
4185 889 597
BseYI C'CCAG_C
Cuts at: 4874 5370 4874
Size: 496 5175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5098
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2427 3121 3882 4910 1540
Size: 275 186 426 694 761 1028 2301
Fragments arranged by size:
2301 1028 761 694 426 275 186
BsiHKAI G_wGCw'C
Cuts at: 2151 3732 3817 4888 2151
Size: 1581 85 1071 2934
Fragments arranged by size:
2934 1581 1071 85
BsmI GAATG_Cn'
Cuts at: 2703 3182 3275 2703
Size: 479 93 5099
Fragments arranged by size:
5099 479 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2716 2834 2887 2990 3732 3817
Size: 399 361 565 118 53 103 742 85
Cuts at: 3817 4888 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 565 399 361 118 103
85 53
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3026 844
Size: 2182 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3558 1394
Size: 429 1735 3507
Fragments arranged by size:
3507 1735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4166 4340 65
Size: 1183 2918 174 1396
Fragments arranged by size:
2918 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4315 4502 1700
Size: 110 2505 187 2869
Fragments arranged by size:
2869 2505 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5671
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstXI CCAn_nnnn'nTGG
Cuts at: 2550 2550
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 2008 2607 2618 3001 3368 3753 3770 5222
Size: 36 599 11 383 367 385 17 1452
Cuts at: 5222 5308 5320 5666 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 599 385 383 367 346 86
36 17 12 11
Bsu36I CC'TnA_GG
Cuts at: 2901 2931 2901
Size: 30 5641
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4180
Fragments arranged by size:
4180 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 3187 3981 4001 3187
Size: 794 20 4857
Fragments arranged by size:
4857 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 3118 4007
Size: 54 1425 50 275 18 378 910 889
Cuts at: 4007 5636 8
Size: 1629 43
Fragments arranged by size:
1629 1425 910 889 378 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2231 3601 1353
Size: 878 1370 3423
Fragments arranged by size:
3423 1370 878
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4258 4632 4778 387
Size: 1064 138 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2492 2861 2885 2963 3734 4331 5118
Size: 393 860 369 24 78 771 597 787
Cuts at: 5118 5386 1239
Size: 268 1524
Fragments arranged by size:
1524 860 787 771 597 393 369 268
78 24
EcoO109I rG'GnC_Cy
Cuts at: 2446 2712 2713 2446
Size: 266 1 5404
Fragments arranged by size:
5404 266 1
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2942 2942
Size: 5671
FspI TGC'GCA
Cuts at: 2174 4177 2174
Size: 2003 3668
HaeII r_GCGC'y
Cuts at: 1678 2349 2365 4818 1678
Size: 671 16 2453 2531
Fragments arranged by size:
2531 2453 671 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4312 4344 4386 4418 1373
Size: 32 135 32 2740 32 42 32 2626
Fragments arranged by size:
2740 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3261 669
Size: 988 281 1077 246 3079
Fragments arranged by size:
3079 1077 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2395 2996 2395
Size: 601 5070
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2036 2104 4785 4969 1305
Size: 731 68 2681 184 2007
Fragments arranged by size:
2681 2007 731 184 68
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3849
Fragments arranged by size:
3849 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3629 3988 4147 518
Size: 648 2463 359 159 2042
Fragments arranged by size:
2463 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2397 2423 2635 3087 3764 4912 5157 1606
Size: 791 26 212 452 677 1148 245 2120
Fragments arranged by size:
2120 1148 791 677 452 245 212 26
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4180
Fragments arranged by size:
4180 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 2173 2779 3029 4574 1132
Size: 97 944 606 250 1545 2229
Fragments arranged by size:
2229 1545 944 606 250 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5671
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5671
PfoI T'CCnGG_A
Cuts at: 2621 2621
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2171 2203 2465 2497 3740 3772 5253 5285 2171
Size: 32 262 32 1243 32 1481 32 2557
Fragments arranged by size:
2557 1481 1243 262 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PspOMI G'GGCC_C
Cuts at: 2712 2712
Size: 5671
PstI C_TGCA'G
Cuts at: 830 2892 3023 830
Size: 2062 131 3478
Fragments arranged by size:
3478 2062 131
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 2888 3019 4154 4835 5026 826
Size: 219 1843 131 1135 681 191 1471
Fragments arranged by size:
1843 1471 1135 681 219 191 131
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3775 4676 4938 820
Size: 197 926 1832 901 262 1553
Fragments arranged by size:
1832 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 2173 3029 1132
Size: 1041 856 3774
Fragments arranged by size:
3774 1041 856
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 2331 1517
Size: 814 4857
StyI C'CwwG_G
Cuts at: 513 1065 2004 3110 513
Size: 552 939 1106 3074
Fragments arranged by size:
3074 1106 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3698 3715 3868 4053 4498 325
Size: 1255 2118 17 153 185 445 1498
Fragments arranged by size:
2118 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3917 5629 96
Size: 274 80 33 51 1443 1940 1712 138
Fragments arranged by size:
1940 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2470 2502 2599 2631 5631 5663 2470
Size: 32 97 32 3000 32 2478
Fragments arranged by size:
3000 2478 97 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 3800 3800
Size: 5671
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI