Restriction map for
pFN21AE9502
(Circular) (Six-base) MAPSORT of: pFN21AE9502.seq Check: 4497 from: 1 to: 5704
pFN21AE9502 5704 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2328 2328
Size: 5704
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3514 278
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2373 2565 2646 3047 1937
Size: 436 192 81 401 4594
Fragments arranged by size:
4594 436 401 192 81
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3767 5151 5419 1632
Size: 2135 1384 268 1917
Fragments arranged by size:
2135 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 2247 2649 4603 5580 1203
Size: 1044 402 1954 977 1327
Fragments arranged by size:
1954 1327 1044 977 402
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2378 2594 3011 1184
Size: 1194 216 417 3877
Fragments arranged by size:
3877 1194 417 216
AlwNI CAG_nnn'CTG
Cuts at: 3447 5019 3447
Size: 1572 4132
ApaLI G'TGCA_C
Cuts at: 2680 3761 4917 2680
Size: 1081 1156 3467
Fragments arranged by size:
3467 1156 1081
ApoI r'AATT_y
Cuts at: 1493 3013 3239 3391 5500 1493
Size: 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 2615 3029 1870
Size: 73 672 414 4545
Fragments arranged by size:
4545 672 414 73
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 2069 2221 3025 4480
Size: 325 217 627 232 50 152 804 1455
Cuts at: 4480 618
Size: 1842
Fragments arranged by size:
1842 1455 804 627 325 232 217 152
50
BanII G_rGCy'C
Cuts at: 1391 2031 2048 2283 3023 1391
Size: 640 17 235 740 4072
Fragments arranged by size:
4072 740 640 235 17
BbeI G_GCGC'C
Cuts at: 2225 2225
Size: 5704
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2240 928
Size: 971 341 4392
Fragments arranged by size:
4392 971 341
BbvCI CC'TCA_GC
Cuts at: 2915 2915
Size: 5704
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3895 3929 1903
Size: 34 50 34 1874 34 3678
Fragments arranged by size:
3678 1874 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4315 136
Size: 107 122 71 631 605 337 2306 1525
Fragments arranged by size:
2306 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5699 5699
Size: 5704
BlpI GC'TnA_GC
Cuts at: 2600 3121 2600
Size: 521 5183
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2684 3765 4921 1790
Size: 232 662 1081 1156 2573
Fragments arranged by size:
2573 1156 1081 662 232
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3435 4393 442
Size: 416 53 83 399 24 2018 958 1753
Fragments arranged by size:
2018 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2377 2763 4364 1239
Size: 1138 386 1601 2579
Fragments arranged by size:
2579 1601 1138 386
Bpu10I CC'TnA_GC
Cuts at: 2915 2915
Size: 5704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3829 4694 4992 3829
Size: 865 298 4541
Fragments arranged by size:
4541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4367 882
Size: 617 2868 2219
Fragments arranged by size:
2868 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3495 493
Size: 711 2291 2702
Fragments arranged by size:
2702 2291 711
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2222 2523 2562 2825
Size: 53 83 186 936 689 301 39 263
Cuts at: 2825 3511 3893 4528 275
Size: 686 382 635 1451
Fragments arranged by size:
1451 936 689 686 635 382 301 263
186 83 53 39
BsaWI w'CCGG_w
Cuts at: 2973 4137 4535 4809 4956 5367 2973
Size: 1164 398 274 147 411 3310
Fragments arranged by size:
3310 1164 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2375 2405 1178
Size: 30 165 30 972 30 4477
Fragments arranged by size:
4477 972 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2804 1186
Size: 1618 4086
BseYI C'CCAG_C
Cuts at: 2015 2518 4907 5403 2015
Size: 503 2389 496 2316
Fragments arranged by size:
2389 2316 503 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5131
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2199 3154 3915 4943 1540
Size: 275 186 198 955 761 1028 2301
Fragments arranged by size:
2301 1028 955 761 275 198 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2319 2684 2697 3765 3850 4921 2145
Size: 174 365 13 1068 85 1071 2928
Fragments arranged by size:
2928 1071 1068 365 174 85 13
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
BsmBI CGTCTCn'nnnn_
Cuts at: 2083 2083
Size: 5704
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2031 2048 2145 2283 2319 2684
Size: 399 232 9 17 97 138 36 365
Cuts at: 2684 2697 3023 3765 3850 4921 1391
Size: 13 326 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 365 326 232 138
97 85 36 17 13 9
BspEI T'CCGG_A
Cuts at: 2973 5367 2973
Size: 2394 3310
BspHI T'CATG_A
Cuts at: 3593 5323 3593
Size: 1730 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2328 2572 2611 2874 3059 844
Size: 1484 244 39 263 185 3489
Fragments arranged by size:
3489 1484 263 244 185 39
BsrBI CCG'CTC
Cuts at: 1394 1823 2739 3591 1394
Size: 429 916 852 3507
Fragments arranged by size:
3507 916 852 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4199 4373 65
Size: 1183 2951 174 1396
Fragments arranged by size:
2951 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4348 4535 1700
Size: 110 2538 187 2869
Fragments arranged by size:
2869 2538 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5704
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 2929 3764 4776 2929
Size: 835 1012 3857
Fragments arranged by size:
3857 1012 835
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2323 794
Size: 1529 4175
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 2239 2776 1184
Size: 1055 537 4112
Fragments arranged by size:
4112 1055 537
BstYI r'GATC_y
Cuts at: 1972 2166 3034 3401 3786 3803 5255 5341 5353
Size: 194 868 367 385 17 1452 86 12
Cuts at: 5353 5699 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 868 385 367 346 194 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2216 2216
Size: 5704
BtgI C'CryG_G
Cuts at: 513 1065 2609 2859 513
Size: 552 1544 250 3358
Fragments arranged by size:
3358 1544 552 250
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2293 487
Size: 683 56 1067 3898
Fragments arranged by size:
3898 1067 683 56
BtsI GCAGTG_nn'
Cuts at: 2123 3220 4014 4034 2123
Size: 1097 794 20 3793
Fragments arranged by size:
3793 1097 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4711 809
Size: 507 3395 1802
Fragments arranged by size:
3395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2196 2265
Size: 54 1425 50 275 18 268 98 69
Cuts at: 2265 2367 2445 2802 3151 4040 5669 8
Size: 102 78 357 349 889 1629 43
Fragments arranged by size:
1629 1425 889 357 349 275 268 102
98 78 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2196 3151 1537
Size: 275 384 955 4090
Fragments arranged by size:
4090 955 384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2288 3634 1353
Size: 935 1346 3423
Fragments arranged by size:
3423 1346 935
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2785 4291 4665 4811 387
Size: 1064 138 1196 1506 374 146 1280
Fragments arranged by size:
1506 1280 1196 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2377 2763 3767 4364 5151 5419 1239
Size: 393 745 386 1004 597 787 268 1524
Fragments arranged by size:
1524 1004 787 745 597 393 386 268
EcoO109I rG'GnC_Cy
Cuts at: 2457 2584 2457
Size: 127 5577
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FspI TGC'GCA
Cuts at: 2714 4210 2714
Size: 1496 4208
HaeII r_GCGC'y
Cuts at: 1678 2225 4851 1678
Size: 547 2626 2531
Fragments arranged by size:
2626 2531 547
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4345 4377 4419 4451 1373
Size: 32 135 32 2773 32 42 32 2626
Fragments arranged by size:
2773 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2305 2374 3048 3294 669
Size: 988 281 367 69 674 246 3079
Fragments arranged by size:
3079 988 674 367 281 246 69
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KasI G'GCGC_C
Cuts at: 2221 2221
Size: 5704
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4818 5002 1305
Size: 3513 184 2007
Fragments arranged by size:
3513 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2100 2447 10
Size: 54 1425 343 268 347 3267
Fragments arranged by size:
3267 1425 347 343 268 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2690 2891 3662 4021 4180 518
Size: 648 1524 201 771 359 159 2042
Fragments arranged by size:
2042 1524 771 648 359 201 159
MspA1I CmG'CkG
Cuts at: 1606 2015 3120 3797 4945 5190 1606
Size: 409 1105 677 1148 245 2120
Fragments arranged by size:
2120 1148 1105 677 409 245
NarI GG'CG_CC
Cuts at: 2222 2222
Size: 5704
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5152
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 2470 3062 4607 1132
Size: 97 1241 592 1545 2229
Fragments arranged by size:
2229 1545 1241 592 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2594 1108
Size: 1486 4218
PfoI T'CCnGG_A
Cuts at: 2162 2162
Size: 5704
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2457 2584 2457
Size: 127 5577
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PstI C_TGCA'G
Cuts at: 830 2328 2583 3056 830
Size: 1498 255 473 3478
Fragments arranged by size:
3478 1498 473 255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 2372 3046 1936
Size: 436 674 4594
Fragments arranged by size:
4594 674 436
SapI GCTCTTCn'nnn_
Cuts at: 2288 2288
Size: 5704
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 2330 3952 5664 2330
Size: 1622 1712 2370
Fragments arranged by size:
2370 1712 1622
SexAI A'CCwGG_T
Cuts at: 2433 2433
Size: 5704
SfcI C'TryA_G
Cuts at: 826 1045 2324 2566 2579 3052 4187 4868 5059
Size: 219 1279 242 13 473 1135 681 191
Cuts at: 5059 826
Size: 1471
Fragments arranged by size:
1471 1279 1135 681 473 242 219 191
13
SfoI GGC'GCC
Cuts at: 2223 2223
Size: 5704
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3808 4709 4971 820
Size: 197 926 1865 901 262 1553
Fragments arranged by size:
1865 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5704
StyI C'CwwG_G
Cuts at: 513 1065 2748 3143 513
Size: 552 1683 395 3074
Fragments arranged by size:
3074 1683 552 395
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2037 2057 2442 3731 3748 3901 4086
Size: 1255 457 20 385 1289 17 153 185
Cuts at: 4086 4531 325
Size: 445 1498
Fragments arranged by size:
1498 1289 1255 457 445 385 185 153
20 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 2328 3950
Size: 274 80 33 51 1443 180 171 1622
Cuts at: 3950 5662 96
Size: 1712 138
Fragments arranged by size:
1712 1622 1443 274 180 171 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2115 2147 5664 5696 2115
Size: 32 3517 32 2123
Fragments arranged by size:
3517 2123 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 2959 3040 2959
Size: 81 5623
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 2942 2981 3833 2942
Size: 39 852 4813
Fragments arranged by size:
4813 852 39
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3512 276
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SfiI SrfI SwaI XcmI