Restriction map for
pFN21AE9464
(Circular) (Six-base) MAPSORT of: pFN21AE9464.seq Check: 3162 from: 1 to: 5824
pFN21AE9464 5824 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2616 3167 1937
Size: 679 551 4594
Fragments arranged by size:
4594 679 551
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2672 2791 3887 5271 5539 1632
Size: 1040 119 1096 1384 268 1917
Fragments arranged by size:
1917 1384 1096 1040 268 119
AfeI AGC'GCT
Cuts at: 2049 3104 2049
Size: 1055 4769
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 2691 4723 5700 1203
Size: 1488 2032 977 1327
Fragments arranged by size:
2032 1488 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 2471 1166
Size: 1305 4519
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2684 1184
Size: 1500 4324
AlwNI CAG_nnn'CTG
Cuts at: 2598 2734 2978 3567 5139 2598
Size: 136 244 589 1572 3283
Fragments arranged by size:
3283 1572 589 244 136
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 3133 3359 3511 5620 1493
Size: 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2208 2292 3004 3149 1870
Size: 73 265 84 712 145 4545
Fragments arranged by size:
4545 712 265 145 84 73
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2120 3145 4600 618
Size: 325 217 627 278 55 1025 1455 1842
Fragments arranged by size:
1842 1455 1025 627 325 278 217 55
BanII G_rGCy'C
Cuts at: 1391 2775 2868 3143 1391
Size: 1384 93 275 4072
Fragments arranged by size:
4072 1384 275 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2118 928
Size: 971 219 4634
Fragments arranged by size:
4634 971 219
BbvCI CC'TCA_GC
Cuts at: 2544 2989 2544
Size: 445 5379
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2998 3032 4015 4049 1903
Size: 34 1061 34 983 34 3678
Fragments arranged by size:
3678 1061 983 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2314 3716 4926 2314
Size: 1402 1210 3212
Fragments arranged by size:
3212 1402 1210
BclI T'GATC_A
Cuts at: 1548 2400 1548
Size: 852 4972
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2571 3075 4435
Size: 107 122 71 631 605 899 504 1360
Cuts at: 4435 136
Size: 1525
Fragments arranged by size:
1525 1360 899 631 605 504 122 107
71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 3885 5041 1790
Size: 2095 1156 2573
Fragments arranged by size:
2573 2095 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2149 3555 4513
Size: 416 53 83 399 24 732 1406 958
Cuts at: 4513 442
Size: 1753
Fragments arranged by size:
1753 1406 958 732 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2110 2215 2450 2531 2984 4484 1239
Size: 797 74 105 235 81 453 1500 2579
Fragments arranged by size:
2579 1500 797 453 235 105 81 74
Bpu10I CC'TnA_GC
Cuts at: 2544 2989 2544
Size: 445 5379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3949 4814 5112 3949
Size: 865 298 4661
Fragments arranged by size:
4661 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4487 882
Size: 617 2988 2219
Fragments arranged by size:
2988 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2692 2833 3615 493
Size: 711 1488 141 782 2702
Fragments arranged by size:
2702 1488 782 711 141
BsaBI GATnn'nnATC
Cuts at: 2222 2532 5818 2222
Size: 310 3286 2228
Fragments arranged by size:
3286 2228 310
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3631 4013 4648 275
Size: 53 83 186 936 2098 382 635 1451
Fragments arranged by size:
2098 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2133 4257 4655 4929 5076 5487 2133
Size: 2124 398 274 147 411 2470
Fragments arranged by size:
2470 2124 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2423 2453 2504 2534 1178
Size: 30 165 30 1020 30 51 30 4468
Fragments arranged by size:
4468 1020 165 51 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2695 2924 1186
Size: 847 662 229 4086
Fragments arranged by size:
4086 847 662 229
BseYI C'CCAG_C
Cuts at: 2734 2931 2981 5027 5523 2734
Size: 197 50 2046 496 3035
Fragments arranged by size:
3035 2046 496 197 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5251
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2045 2172 2321 3011 3885 3970 5041 2045
Size: 127 149 690 874 85 1071 2828
Fragments arranged by size:
2828 1071 874 690 149 127 85
BsmI GAATG_Cn'
Cuts at: 3335 3428 3335
Size: 93 5731
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2172 2321 2775 2868 3011 3143
Size: 399 255 127 149 454 93 143 132
Cuts at: 3143 3885 3970 5041 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 255 149 143
132 127 93 85
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3179 844
Size: 2335 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2824 3711 1394
Size: 429 1001 887 3507
Fragments arranged by size:
3507 1001 887 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2286 4319 4493 65
Size: 1183 1038 2033 174 1396
Fragments arranged by size:
2033 1396 1183 1038 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3026 4468 4655 1700
Size: 110 1216 1442 187 2869
Fragments arranged by size:
2869 1442 1216 187 110
BsrGI T'GTAC_A
Cuts at: 96 2920 96
Size: 2824 3000
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2339 2978 794
Size: 1545 639 3640
Fragments arranged by size:
3640 1545 639
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstYI r'GATC_y
Cuts at: 1972 2217 2349 2514 2871 3154 3521 3906 3923
Size: 245 132 165 357 283 367 385 17
Cuts at: 3923 5375 5461 5473 5819 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 357 346 283 245
165 132 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2298 2566 513
Size: 552 939 294 268 3771
Fragments arranged by size:
3771 939 552 294 268
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2072 3037 487
Size: 683 56 846 965 3274
Fragments arranged by size:
3274 965 846 683 56
BtsI GCAGTG_nn'
Cuts at: 2711 3340 4134 4154 2711
Size: 629 794 20 4381
Fragments arranged by size:
4381 794 629 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 2422 2460
Size: 54 1425 50 275 18 552 40 38
Cuts at: 2460 2563 3271 4160 5789 8
Size: 103 708 889 1629 43
Fragments arranged by size:
1629 1425 889 708 552 275 103 54
50 43 40 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 2882 3754 1353
Size: 824 705 872 3423
Fragments arranged by size:
3423 872 824 705
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4411 4785 4931 387
Size: 1064 138 2822 374 146 1280
Fragments arranged by size:
2822 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2110 2215 2450 2531 2672 2791
Size: 393 404 74 105 235 81 141 119
Cuts at: 2791 2984 3887 4484 5271 5539 1239
Size: 193 903 597 787 268 1524
Fragments arranged by size:
1524 903 787 597 404 393 268 235
193 141 119 105 81 74
EcoNI CCTnn'n_nnAGG
Cuts at: 2018 2726 2018
Size: 708 5116
EcoO109I rG'GnC_Cy
Cuts at: 2143 2505 2959 2985 2143
Size: 362 454 26 4982
Fragments arranged by size:
4982 454 362 26
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2893 2893
Size: 5824
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2051 2279 3106 4971 1678
Size: 373 228 827 1865 2531
Fragments arranged by size:
2531 1865 827 373 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2423 2455 4465 4497 4539
Size: 32 135 32 851 32 2010 32 42
Cuts at: 4539 4571 1373
Size: 32 2626
Fragments arranged by size:
2626 2010 851 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3168 3414 669
Size: 988 281 1230 246 3079
Fragments arranged by size:
3079 1230 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2195 748
Size: 1447 4377
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 5122 1305
Size: 3633 184 2007
Fragments arranged by size:
3633 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 2424 2462 2565 10
Size: 54 1425 343 552 40 38 103 3269
Fragments arranged by size:
3269 1425 552 343 103 54 40 38
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 2261 2312 2471 2831 3782 4141
Size: 648 912 183 51 159 360 951 359
Cuts at: 4141 4300 518
Size: 159 2042
Fragments arranged by size:
2042 951 912 648 360 359 183 159
159 51
MspA1I CmG'CkG
Cuts at: 1606 2543 2650 2993 3240 3917 5065 5310 1606
Size: 937 107 343 247 677 1148 245 2120
Fragments arranged by size:
2120 1148 937 677 343 247 245 107
NaeI GCC'GGC
Cuts at: 3028 3028
Size: 5824
NcoI C'CATG_G
Cuts at: 513 1065 2004 2566 513
Size: 552 939 562 3771
Fragments arranged by size:
3771 939 562 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NgoMIV G'CCGG_C
Cuts at: 3026 3026
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 3182 4727 1132
Size: 97 1953 1545 2229
Fragments arranged by size:
2229 1953 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2500 1348
Size: 1152 4672
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5824
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2143 2505 2143
Size: 362 5462
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspXI vC'TCGA_Gb
Cuts at: 3004 3004
Size: 5824
PstI C_TGCA'G
Cuts at: 830 2730 2974 3176 830
Size: 1900 244 202 3478
Fragments arranged by size:
3478 1900 244 202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
PvuII CAG'CTG
Cuts at: 2543 2993 2543
Size: 450 5374
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SanDI GG'GwC_CC
Cuts at: 2505 2505
Size: 5824
SapI GCTCTTCn'nnn_
Cuts at: 2177 2177
Size: 5824
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SfcI C'TryA_G
Cuts at: 826 1045 2726 2970 3172 4307 4988 5179 826
Size: 219 1681 244 202 1135 681 191 1471
Fragments arranged by size:
1681 1471 1135 681 244 219 202 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2571 2571
Size: 5824
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 2294 3151 1872
Size: 422 857 4545
Fragments arranged by size:
4545 857 422
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3004 3928 4829 5091 820
Size: 197 926 1061 924 901 262 1553
Fragments arranged by size:
1553 1061 926 924 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SrfI GCCC'GGGC
Cuts at: 2294 2294
Size: 5824
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5824
StyI C'CwwG_G
Cuts at: 513 1065 2004 2566 3263 513
Size: 552 939 562 697 3074
Fragments arranged by size:
3074 939 697 562 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2513 3851 3868 4021 4206 4651 325
Size: 1255 933 1338 17 153 185 445 1498
Fragments arranged by size:
1498 1338 1255 933 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2802 2920 4070
Size: 274 80 33 51 1443 825 118 1150
Cuts at: 4070 5782 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1150 825 274 138 118 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5784 5816 5784
Size: 32 5792
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 3004 1943
Size: 1061 4763
XmaI C'CCGG_G
Cuts at: 1870 2292 3149 1870
Size: 422 857 4545
Fragments arranged by size:
4545 857 422
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspOMI PsrI RsrII SacI SacII SexAI SfoI
SwaI