Restriction map for
pFN21AE9460
(Circular) (Six-base) MAPSORT of: pFN21AE9460.seq Check: 2035 from: 1 to: 5662
pFN21AE9460 5662 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3472 278
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2741 3005 1937
Size: 804 264 4594
Fragments arranged by size:
4594 804 264
Acc65I G'GTAC_C
Cuts at: 2983 2983
Size: 5662
AclI AA'CG_TT
Cuts at: 1563 3789 4162 1563
Size: 2226 373 3063
Fragments arranged by size:
3063 2226 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2270 2330 3725 5109 5377 1632
Size: 638 60 1395 1384 268 1917
Fragments arranged by size:
1917 1395 1384 638 268 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5465
AflIII A'CryG_T
Cuts at: 1203 4561 5538 1203
Size: 3358 977 1327
Fragments arranged by size:
3358 1327 977
AgeI A'CCGG_T
Cuts at: 4493 4493
Size: 5662
AhdI GACnn_n'nnGTC
Cuts at: 4391 4391
Size: 5662
AleI CACnn'nnGTG
Cuts at: 1166 2145 1166
Size: 979 4683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5662
AlwNI CAG_nnn'CTG
Cuts at: 3405 4977 3405
Size: 1572 4090
ApaLI G'TGCA_C
Cuts at: 3719 4875 3719
Size: 1156 4506
ApoI r'AATT_y
Cuts at: 1493 2516 2545 2572 2971 3197 3349 5458 1493
Size: 1023 29 27 399 226 152 2109 1697
Fragments arranged by size:
2109 1697 1023 399 226 152 29 27
AseI AT'TA_AT
Cuts at: 160 1032 2166 4216 160
Size: 872 1134 2050 1606
Fragments arranged by size:
2050 1606 1134 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2987 1870
Size: 73 1044 4545
Fragments arranged by size:
4545 1044 73
BamHI G'GATC_C
Cuts at: 2992 3359 2992
Size: 367 5295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 2443 2983 4438 618
Size: 325 217 627 449 207 540 1455 1842
Fragments arranged by size:
1842 1455 627 540 449 325 217 207
BanII G_rGCy'C
Cuts at: 1391 2981 1391
Size: 1590 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2336 928
Size: 971 437 4254
Fragments arranged by size:
4254 971 437
BbvCI CC'TCA_GC
Cuts at: 2747 2747
Size: 5662
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3853 3887 1903
Size: 34 1916 34 3678
Fragments arranged by size:
3678 1916 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3554 4764 3554
Size: 1210 4452
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5662
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2807 4273 136
Size: 107 122 71 631 605 1135 1466 1525
Fragments arranged by size:
1525 1466 1135 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2041 2367 5657 2041
Size: 326 3290 2046
Fragments arranged by size:
3290 2046 326
BlpI GC'TnA_GC
Cuts at: 3079 3079
Size: 5662
Bme1580I G_kGCm'C
Cuts at: 1790 3723 4879 1790
Size: 1933 1156 2573
Fragments arranged by size:
2573 1933 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2024 3393 4351
Size: 416 53 83 399 24 607 1369 958
Cuts at: 4351 442
Size: 1753
Fragments arranged by size:
1753 1369 958 607 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 4322 1239
Size: 827 2256 2579
Fragments arranged by size:
2579 2256 827
Bpu10I CC'TnA_GC
Cuts at: 2747 2747
Size: 5662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3787 4652 4950 3787
Size: 865 298 4499
Fragments arranged by size:
4499 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2912 4325 882
Size: 617 1413 1413 2219
Fragments arranged by size:
2219 1413 1413 617
BsaAI yAC'GTr
Cuts at: 493 1204 2058 3453 493
Size: 711 854 1395 2702
Fragments arranged by size:
2702 1395 854 711
BsaBI GATnn'nnATC
Cuts at: 2462 5656 2462
Size: 3194 2468
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3469 3851 4486 275
Size: 53 83 186 936 1936 382 635 1451
Fragments arranged by size:
1936 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4095 4493 4767 4914 5325 4095
Size: 398 274 147 411 4432
Fragments arranged by size:
4432 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5437
Fragments arranged by size:
5437 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5662
BseYI C'CCAG_C
Cuts at: 4865 5361 4865
Size: 496 5166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5089
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3873 4901 1540
Size: 275 186 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3723 3808 4879 3723
Size: 85 1071 4506
Fragments arranged by size:
4506 1071 85
BsmI GAATG_Cn'
Cuts at: 2876 3173 3266 2876
Size: 297 93 5272
Fragments arranged by size:
5272 297 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2981 3723 3808 4879 1391
Size: 399 1191 742 85 1071 2174
Fragments arranged by size:
2174 1191 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 3551 5281 3551
Size: 1730 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3017 844
Size: 2173 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2883 3549 1394
Size: 429 1060 666 3507
Fragments arranged by size:
3507 1060 666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4157 4331 65
Size: 1183 2909 174 1396
Fragments arranged by size:
2909 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4306 4493 1700
Size: 110 2496 187 2869
Fragments arranged by size:
2869 2496 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5662
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 3722 4734 3722
Size: 1012 4650
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5662
BstBI TT'CG_AA
Cuts at: 4466 4466
Size: 5662
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5662
BstYI r'GATC_y
Cuts at: 1972 2041 2367 2992 3359 3744 3761 5213 5299
Size: 69 326 625 367 385 17 1452 86
Cuts at: 5299 5311 5657 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 625 385 367 346 326 86
69 17 12
BstZ17I GTA'TAC
Cuts at: 2742 2742
Size: 5662
BtgI C'CryG_G
Cuts at: 513 1065 2691 513
Size: 552 1626 3484
Fragments arranged by size:
3484 1626 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4923
Fragments arranged by size:
4923 683 56
BtsI GCAGTG_nn'
Cuts at: 2260 2813 3178 3972 3992 2260
Size: 553 365 794 20 3930
Fragments arranged by size:
3930 794 553 365 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5627
DraI TTT'AAA
Cuts at: 2509 2571 2966 2509
Size: 62 395 5205
Fragments arranged by size:
5205 395 62
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4669 809
Size: 507 3353 1802
Fragments arranged by size:
3353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3109 3998 5627
Size: 54 1425 50 275 18 1279 889 1629
Cuts at: 5627 8
Size: 43
Fragments arranged by size:
1629 1425 1279 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 1537
Size: 275 1297 4090
Fragments arranged by size:
4090 1297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2798 3592 1353
Size: 1445 794 3423
Fragments arranged by size:
3423 1445 794
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4249 4623 4769 387
Size: 1064 138 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2270 2330 3725 4322 5109 5377
Size: 393 434 204 60 1395 597 787 268
Cuts at: 5377 1239
Size: 1524
Fragments arranged by size:
1524 1395 787 597 434 393 268 204
60
EcoRI G'AATT_C
Cuts at: 2971 3349 2971
Size: 378 5284
EcoRV GAT'ATC
Cuts at: 2406 2406
Size: 5662
FspI TGC'GCA
Cuts at: 4168 4168
Size: 5662
HaeII r_GCGC'y
Cuts at: 1678 4809 1678
Size: 3131 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2911 2943 4303 4335 4377
Size: 32 135 32 1339 32 1360 32 42
Cuts at: 4377 4409 1373
Size: 32 2626
Fragments arranged by size:
2626 1360 1339 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2224 3006 3252 669
Size: 988 281 286 782 246 3079
Fragments arranged by size:
3079 988 782 286 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5662
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KpnI G_GTAC'C
Cuts at: 2987 2987
Size: 5662
MfeI C'AATT_G
Cuts at: 2599 3261 2599
Size: 662 5000
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2065 4776 4960 1305
Size: 760 2711 184 2007
Fragments arranged by size:
2711 2007 760 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3840
Fragments arranged by size:
3840 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2145 3620 3979 4138 518
Size: 648 979 1475 359 159 2042
Fragments arranged by size:
2042 1475 979 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2340 2350 2361 3078 3755 4903 5148 1606
Size: 734 10 11 717 677 1148 245 2120
Fragments arranged by size:
2120 1148 734 717 677 245 11 10
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5110
NdeI CA'TA_TG
Cuts at: 387 2552 2705 387
Size: 2165 153 3344
Fragments arranged by size:
3344 2165 153
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5662
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 3020 4565 1132
Size: 97 1791 1545 2229
Fragments arranged by size:
2229 1791 1545 97
PacI TTA_AT'TAA
Cuts at: 2166 2166
Size: 5662
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5662
PciI A'CATG_T
Cuts at: 4561 4561
Size: 5662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5662
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 5244 5276 3731
Size: 32 1481 32 4117
Fragments arranged by size:
4117 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2340 2759 1613
Size: 727 419 4516
Fragments arranged by size:
4516 727 419
PsiI TTA'TAA
Cuts at: 2037 2438 3232 2037
Size: 401 794 4467
Fragments arranged by size:
4467 794 401
PstI C_TGCA'G
Cuts at: 830 3014 830
Size: 2184 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
PvuII CAG'CTG
Cuts at: 2350 2361 2350
Size: 11 5651
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SbfI CC_TGCA'GG
Cuts at: 3014 3014
Size: 5662
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SfcI C'TryA_G
Cuts at: 826 1045 2623 3010 4145 4826 5017 826
Size: 219 1578 387 1135 681 191 1471
Fragments arranged by size:
1578 1471 1135 681 387 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2989 1872
Size: 1117 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3766 4667 4929 820
Size: 197 926 1823 901 262 1553
Fragments arranged by size:
1823 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3453 493
Size: 2960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 3020 1132
Size: 1888 3774
SspI AAT'ATT
Cuts at: 5 52 2565 3586 5
Size: 47 2513 1021 2081
Fragments arranged by size:
2513 2081 1021 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5662
StyI C'CwwG_G
Cuts at: 513 1065 2240 2922 3101 513
Size: 552 1175 682 179 3074
Fragments arranged by size:
3074 1175 682 552 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2219 3689 3706 3859 4044 4489 325
Size: 1255 639 1470 17 153 185 445 1498
Fragments arranged by size:
1498 1470 1255 639 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2856 3908 5620
Size: 274 80 33 51 1443 879 1052 1712
Cuts at: 5620 96
Size: 138
Fragments arranged by size:
1712 1443 1052 879 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5622 5654 5622
Size: 32 5630
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5662
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5662
XmaI C'CCGG_G
Cuts at: 1870 2987 1870
Size: 1117 4545
XmnI GAAnn'nnTTC
Cuts at: 2255 3791 2255
Size: 1536 4126
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3470 276
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PacI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI