Restriction map for
pFN21AE9458
(Circular) (Six-base) MAPSORT of: pFN21AE9458.seq Check: 9050 from: 1 to: 6898
pFN21AE9458 6898 bp
With 182 enzymes: SgfI *
March 28, 2012 12:10 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4708 278
Size: 53 83 186 936 3172 2468
Fragments arranged by size:
3172 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4241 1937
Size: 2304 4594
Acc65I G'GTAC_C
Cuts at: 4219 4219
Size: 6898
AclI AA'CG_TT
Cuts at: 1563 5025 5398 1563
Size: 3462 373 3063
Fragments arranged by size:
3462 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2880 3549 4961 6345 6613 1632
Size: 1248 669 1412 1384 268 1917
Fragments arranged by size:
1917 1412 1384 1248 669 268
AfeI AGC'GCT
Cuts at: 2933 2952 2933
Size: 19 6879
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6701
AflIII A'CryG_T
Cuts at: 1203 2123 5797 6774 1203
Size: 920 3674 977 1327
Fragments arranged by size:
3674 1327 977 920
AgeI A'CCGG_T
Cuts at: 5729 5729
Size: 6898
AhdI GACnn_n'nnGTC
Cuts at: 3904 5627 3904
Size: 1723 5175
AleI CACnn'nnGTG
Cuts at: 1166 2412 3833 3845 1166
Size: 1246 1421 12 4219
Fragments arranged by size:
4219 1421 1246 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6898
AlwNI CAG_nnn'CTG
Cuts at: 4641 6213 4641
Size: 1572 5326
ApaLI G'TGCA_C
Cuts at: 2317 4955 6111 2317
Size: 2638 1156 3104
Fragments arranged by size:
3104 2638 1156
ApoI r'AATT_y
Cuts at: 1493 2697 3197 3271 3873 4207 4433 4585 6694
Size: 1204 500 74 602 334 226 152 2109
Cuts at: 6694 1493
Size: 1697
Fragments arranged by size:
2109 1697 1204 602 500 334 226 152
74
AseI AT'TA_AT
Cuts at: 160 1032 4059 5452 160
Size: 872 3027 1393 1606
Fragments arranged by size:
3027 1606 1393 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6898
AvaI C'yCGr_G
Cuts at: 1870 1943 2779 4223 1870
Size: 73 836 1444 4545
Fragments arranged by size:
4545 1444 836 73
AvrII C'CTAG_G
Cuts at: 4000 4000
Size: 6898
BamHI G'GATC_C
Cuts at: 4228 4595 4228
Size: 367 6531
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2522 2897 4219 5674 618
Size: 325 217 627 735 375 1322 1455 1842
Fragments arranged by size:
1842 1455 1322 735 627 375 325 217
BanII G_rGCy'C
Cuts at: 1391 2816 4217 1391
Size: 1425 1401 4072
Fragments arranged by size:
4072 1425 1401
BbeI G_GCGC'C
Cuts at: 2526 2526
Size: 6898
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3550 928
Size: 971 1651 4276
Fragments arranged by size:
4276 1651 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5089 5123 1903
Size: 34 3152 34 3678
Fragments arranged by size:
3678 3152 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4790 6000 4790
Size: 1210 5688
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6898
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2855 5509 136
Size: 107 122 71 631 605 1183 2654 1525
Fragments arranged by size:
2654 1525 1183 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3574 3947 6893 3574
Size: 373 2946 3579
Fragments arranged by size:
3579 2946 373
BlpI GC'TnA_GC
Cuts at: 4315 4315
Size: 6898
Bme1580I G_kGCm'C
Cuts at: 1790 2321 4959 6115 1790
Size: 531 2638 1156 2573
Fragments arranged by size:
2638 2573 1156 531
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6898
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3074 3595 4629
Size: 416 53 83 399 24 1657 521 1034
Cuts at: 4629 5587 442
Size: 958 1753
Fragments arranged by size:
1753 1657 1034 958 521 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6898
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2419 2548 2757 3738 3952 5558 1239
Size: 1180 129 209 981 214 1606 2579
Fragments arranged by size:
2579 1606 1180 981 214 209 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5023 5888 6186 5023
Size: 865 298 5735
Fragments arranged by size:
5735 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5561 882
Size: 617 4062 2219
Fragments arranged by size:
4062 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2575 4689 493
Size: 711 1371 2114 2702
Fragments arranged by size:
2702 2114 1371 711
BsaBI GATnn'nnATC
Cuts at: 3952 6892 3952
Size: 2940 3958
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2523 3161 4705 5087
Size: 53 83 186 936 990 638 1544 382
Cuts at: 5087 5722 275
Size: 635 1451
Fragments arranged by size:
1544 1451 990 936 638 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3632 5331 5729 6003 6150 6561 3632
Size: 1699 398 274 147 411 3969
Fragments arranged by size:
3969 1699 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6673
Fragments arranged by size:
6673 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2606 2678 1186
Size: 1420 72 5406
Fragments arranged by size:
5406 1420 72
BseYI C'CCAG_C
Cuts at: 2517 2852 6101 6597 2517
Size: 335 3249 496 2818
Fragments arranged by size:
3249 2818 496 335
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6325
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4348 5109 6137 1540
Size: 275 186 2347 761 1028 2301
Fragments arranged by size:
2347 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2321 2457 2816 4959 5044 6115 2321
Size: 136 359 2143 85 1071 3104
Fragments arranged by size:
3104 2143 1071 359 136 85
BsmI GAATG_Cn'
Cuts at: 3662 4409 4502 3662
Size: 747 93 6058
Fragments arranged by size:
6058 747 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2321 2457 2816 4217 4959 5044 6115
Size: 399 531 136 359 1401 742 85 1071
Cuts at: 6115 1391
Size: 2174
Fragments arranged by size:
2174 1401 1071 742 531 399 359 136
85
BspEI T'CCGG_A
Cuts at: 6561 6561
Size: 6898
BspHI T'CATG_A
Cuts at: 2130 2241 3553 4787 6517 2130
Size: 111 1312 1234 1730 2511
Fragments arranged by size:
2511 1730 1312 1234 111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4253 844
Size: 3409 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4785 1394
Size: 429 2962 3507
Fragments arranged by size:
3507 2962 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5393 5567 65
Size: 1183 4145 174 1396
Fragments arranged by size:
4145 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5542 5729 1700
Size: 110 3732 187 2869
Fragments arranged by size:
3732 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6898
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6898
BssSI C'ACGA_G
Cuts at: 2618 4958 5970 2618
Size: 2340 1012 3546
Fragments arranged by size:
3546 2340 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6898
BstBI TT'CG_AA
Cuts at: 5702 5702
Size: 6898
BstEII G'GTnAC_C
Cuts at: 1184 2257 1184
Size: 1073 5825
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 3835 2215
Size: 1620 5278
BstYI r'GATC_y
Cuts at: 1972 3574 3947 4228 4595 4980 4997 6449 6535
Size: 1602 373 281 367 385 17 1452 86
Cuts at: 6535 6547 6893 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1602 1452 385 373 367 346 281
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2704 513
Size: 552 1639 4707
Fragments arranged by size:
4707 1639 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6159
Fragments arranged by size:
6159 683 56
BtsI GCAGTG_nn'
Cuts at: 2285 3162 4414 5208 5228 2285
Size: 877 1252 794 20 3955
Fragments arranged by size:
3955 1252 877 794 20
ClaI AT'CG_AT
Cuts at: 1554 3781 1554
Size: 2227 4671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6863
DraI TTT'AAA
Cuts at: 2082 4202 2082
Size: 2120 4778
DraIII CAC_nnn'GTG
Cuts at: 2995 2995
Size: 6898
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5905 809
Size: 507 4589 1802
Fragments arranged by size:
4589 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2282 3063 3796
Size: 54 1425 50 275 18 452 781 733
Cuts at: 3796 4345 5234 6863 8
Size: 549 889 1629 43
Fragments arranged by size:
1629 1425 889 781 733 549 452 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4345 1537
Size: 275 2533 4090
Fragments arranged by size:
4090 2533 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3552 3882 4828 1353
Size: 2199 330 946 3423
Fragments arranged by size:
3423 2199 946 330
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2076 5485 5859 6005 387
Size: 1064 138 487 3409 374 146 1280
Fragments arranged by size:
3409 1280 1064 487 374 146 138
EcoICRI GAG'CTC
Cuts at: 2814 2814
Size: 6898
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2419 2548 2757 2880 3549 3738 3952
Size: 393 787 129 209 123 669 189 214
Cuts at: 3952 4961 5558 6345 6613 1239
Size: 1009 597 787 268 1524
Fragments arranged by size:
1524 1009 787 787 669 597 393 268
214 209 189 129 123
EcoO109I rG'GnC_Cy
Cuts at: 2293 2350 2724 2293
Size: 57 374 6467
Fragments arranged by size:
6467 374 57
EcoRI G'AATT_C
Cuts at: 3197 4207 4585 3197
Size: 1010 378 5510
Fragments arranged by size:
5510 1010 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2984 2984
Size: 6898
FspI TGC'GCA
Cuts at: 2534 5404 2534
Size: 2870 4028
FspAI rTGC'GCAy
Cuts at: 2534 2534
Size: 6898
HaeII r_GCGC'y
Cuts at: 1678 2504 2526 2794 2935 2954 6045 1678
Size: 826 22 268 141 19 3091 2531
Fragments arranged by size:
3091 2531 826 268 141 22 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3664 3696 3809 3841 3890
Size: 32 135 32 2092 32 113 32 49
Cuts at: 3890 3922 5539 5571 5613 5645 1373
Size: 32 1617 32 42 32 2626
Fragments arranged by size:
2626 2092 1617 135 113 49 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4242 4488 669
Size: 988 281 2304 246 3079
Fragments arranged by size:
3079 2304 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4161 748
Size: 3413 3485
HpaI GTT'AAC
Cuts at: 4488 4488
Size: 6898
KasI G'GCGC_C
Cuts at: 2522 2522
Size: 6898
KpnI G_GTAC'C
Cuts at: 4223 4223
Size: 6898
MfeI C'AATT_G
Cuts at: 4497 4497
Size: 6898
MluI A'CGCG_T
Cuts at: 6774 6774
Size: 6898
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2709 2841 3483 6012 6196 1305
Size: 1404 132 642 2529 184 2007
Fragments arranged by size:
2529 2007 1404 642 184 132
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2284 3065 3798 10
Size: 54 1425 343 452 781 733 3110
Fragments arranged by size:
3110 1425 781 733 452 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 2267 2412 2984 3833 3845 4856
Size: 648 1047 54 145 572 849 12 1011
Cuts at: 4856 5215 5374 518
Size: 359 159 2042
Fragments arranged by size:
2042 1047 1011 849 648 572 359 159
145 54 12
MspA1I CmG'CkG
Cuts at: 1606 2509 2846 2852 4314 4991 6139 6384 1606
Size: 903 337 6 1462 677 1148 245 2120
Fragments arranged by size:
2120 1462 1148 903 677 337 245 6
NarI GG'CG_CC
Cuts at: 2523 2523
Size: 6898
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6346
NdeI CA'TA_TG
Cuts at: 387 3151 387
Size: 2764 4134
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6898
NotI GC'GGCC_GC
Cuts at: 4345 4345
Size: 6898
NspI r_CATG'y
Cuts at: 1132 1229 2127 4256 5801 1132
Size: 97 898 2129 1545 2229
Fragments arranged by size:
2229 2129 1545 898 97
PasI CC'CwG_GG
Cuts at: 1348 2541 1348
Size: 1193 5705
PciI A'CATG_T
Cuts at: 2123 5797 2123
Size: 3674 3224
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3102 1108
Size: 1994 4904
PfoI T'CCnGG_A
Cuts at: 3968 3968
Size: 6898
PmeI GTTT'AAAC
Cuts at: 4202 4202
Size: 6898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4967 4999 6480 6512 4967
Size: 32 1481 32 5353
Fragments arranged by size:
5353 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2724 2293
Size: 431 6467
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6898
PsiI TTA'TAA
Cuts at: 3234 4468 3234
Size: 1234 5664
PstI C_TGCA'G
Cuts at: 830 2870 3140 4250 830
Size: 2040 270 1110 3478
Fragments arranged by size:
3478 2040 1110 270
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6898
RsrII CG'GwC_CG
Cuts at: 2831 2831
Size: 6898
SacI G_AGCT'C
Cuts at: 2816 2816
Size: 6898
SalI G'TCGA_C
Cuts at: 1936 4240 1936
Size: 2304 4594
SanDI GG'GwC_CC
Cuts at: 2724 2724
Size: 6898
SbfI CC_TGCA'GG
Cuts at: 2870 4250 2870
Size: 1380 5518
ScaI AGT'ACT
Cuts at: 5146 6858 5146
Size: 1712 5186
SfcI C'TryA_G
Cuts at: 826 1045 2866 3136 3693 4246 5381 6062 6253
Size: 219 1821 270 557 553 1135 681 191
Cuts at: 6253 826
Size: 1471
Fragments arranged by size:
1821 1471 1135 681 557 553 270 219
191
SfoI GGC'GCC
Cuts at: 2524 2524
Size: 6898
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6898
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6898
SmaI CCC'GGG
Cuts at: 1872 4225 1872
Size: 2353 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5002 5903 6165 820
Size: 197 926 3059 901 262 1553
Fragments arranged by size:
3059 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2575 4689 493
Size: 2082 2114 2702
Fragments arranged by size:
2702 2114 2082
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6898
SphI G_CATG'C
Cuts at: 1132 4256 1132
Size: 3124 3774
SspI AAT'ATT
Cuts at: 5 52 4822 5
Size: 47 4770 2081
Fragments arranged by size:
4770 2081 47
StuI AGG'CCT
Cuts at: 1517 3893 1517
Size: 2376 4522
StyI C'CwwG_G
Cuts at: 513 1065 2145 3030 3894 4000 4337 513
Size: 552 1080 885 864 106 337 3074
Fragments arranged by size:
3074 1080 885 864 552 337 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4925 4942 5095 5280 5725 325
Size: 1255 3345 17 153 185 445 1498
Fragments arranged by size:
3345 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5144 6856 96
Size: 274 80 33 51 1443 3167 1712 138
Fragments arranged by size:
3167 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2604 2636 4027 4059 6858 6890 2604
Size: 32 1391 32 2799 32 2612
Fragments arranged by size:
2799 2612 1391 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3676 1316
Size: 2360 4538
XbaI T'CTAG_A
Cuts at: 4234 4234
Size: 6898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3116 3221 3385 3116
Size: 105 164 6629
Fragments arranged by size:
6629 164 105
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6898
XmaI C'CCGG_G
Cuts at: 1870 4223 1870
Size: 2353 4545
XmnI GAAnn'nnTTC
Cuts at: 2933 5027 2933
Size: 2094 4804
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4706 276
Size: 53 83 186 936 3172 2468
Fragments arranged by size:
3172 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
RsrII SacI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII SacII SapI SexAI SfiI SrfI SwaI