Restriction map for
pFN21AE9455
(Circular) (Six-base) MAPSORT of: pFN21AE9455.seq Check: 1611 from: 1 to: 6289
pFN21AE9455 6289 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2098 2098
Size: 6289
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4099 278
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3042 3144 3632 1937
Size: 1105 102 488 4594
Fragments arranged by size:
4594 1105 488 102
Acc65I G'GTAC_C
Cuts at: 3610 3610
Size: 6289
AclI AA'CG_TT
Cuts at: 1563 4416 4789 1563
Size: 2853 373 3063
Fragments arranged by size:
3063 2853 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2306 3550 4352 5736 6004 1632
Size: 674 1244 802 1384 268 1917
Fragments arranged by size:
1917 1384 1244 802 674 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6092
AflIII A'CryG_T
Cuts at: 1203 3204 5188 6165 1203
Size: 2001 1984 977 1327
Fragments arranged by size:
2001 1984 1327 977
AgeI A'CCGG_T
Cuts at: 5120 5120
Size: 6289
AhdI GACnn_n'nnGTC
Cuts at: 5018 5018
Size: 6289
AleI CACnn'nnGTG
Cuts at: 1166 2016 1166
Size: 850 5439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6289
AlwNI CAG_nnn'CTG
Cuts at: 2230 2532 3360 4032 5604 2230
Size: 302 828 672 1572 2915
Fragments arranged by size:
2915 1572 828 672 302
ApaI G_GGCC'C
Cuts at: 2479 3100 2479
Size: 621 5668
ApaLI G'TGCA_C
Cuts at: 3236 4346 5502 3236
Size: 1110 1156 4023
Fragments arranged by size:
4023 1156 1110
ApoI r'AATT_y
Cuts at: 1493 3598 3824 3976 6085 1493
Size: 2105 226 152 2109 1697
Fragments arranged by size:
2109 2105 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4843 160
Size: 872 3811 1606
Fragments arranged by size:
3811 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
AvaI C'yCGr_G
Cuts at: 1870 1943 2319 2358 2933 3032 3614 1870
Size: 73 376 39 575 99 582 4545
Fragments arranged by size:
4545 582 575 376 99 73 39
BamHI G'GATC_C
Cuts at: 2512 3619 3986 2512
Size: 1107 367 4815
Fragments arranged by size:
4815 1107 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 2293 2740 3525 3610
Size: 325 217 627 231 275 447 785 85
Cuts at: 3610 5065 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 785 627 447 325 275 231
217 85
BanII G_rGCy'C
Cuts at: 1391 2129 2479 2621 2909 3100 3140 3230 3608
Size: 738 350 142 288 191 40 90 378
Cuts at: 3608 1391
Size: 4072
Fragments arranged by size:
4072 738 378 350 288 191 142 90
40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2566 3160 928
Size: 971 667 594 4057
Fragments arranged by size:
4057 971 667 594
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2764 2798 4480 4514 1903
Size: 34 827 34 1682 34 3678
Fragments arranged by size:
3678 1682 827 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4181 5391 4181
Size: 1210 5079
BclI T'GATC_A
Cuts at: 1548 2796 1548
Size: 1248 5041
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2553 2625 4900
Size: 107 122 71 631 605 881 72 2275
Cuts at: 4900 136
Size: 1525
Fragments arranged by size:
2275 1525 881 631 605 122 107 72
71
BglII A'GATC_T
Cuts at: 6284 6284
Size: 6289
BlpI GC'TnA_GC
Cuts at: 2243 2903 2981 3706 2243
Size: 660 78 725 4826
Fragments arranged by size:
4826 725 660 78
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2298 2479 2496 2934 3100 3240 4350
Size: 233 275 181 17 438 166 140 1110
Cuts at: 4350 5506 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1110 438 275 233 181 166
140 17
BmgBI CAC'GTC
Cuts at: 1318 3273 1318
Size: 1955 4334
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4020 4978 442
Size: 416 53 83 399 24 2603 958 1753
Fragments arranged by size:
2603 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 6289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2635 4949 1239
Size: 941 455 2314 2579
Fragments arranged by size:
2579 2314 941 455
Bpu10I CC'TnA_GC
Cuts at: 2087 2087
Size: 6289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4414 5279 5577 4414
Size: 865 298 5126
Fragments arranged by size:
5126 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4952 882
Size: 617 3453 2219
Fragments arranged by size:
3453 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2497 2751 4080 493
Size: 711 1293 254 1329 2702
Fragments arranged by size:
2702 1329 1293 711 254
BsaBI GATnn'nnATC
Cuts at: 3002 6283 3002
Size: 3281 3008
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2364 4096 4478 5113
Size: 53 83 186 936 831 1732 382 635
Cuts at: 5113 275
Size: 1451
Fragments arranged by size:
1732 1451 936 831 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4722 5120 5394 5541 5952 4722
Size: 398 274 147 411 5059
Fragments arranged by size:
5059 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6064
Fragments arranged by size:
6064 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3017 3020 3562 1186
Size: 1831 3 542 3913
Fragments arranged by size:
3913 1831 542 3
BseYI C'CCAG_C
Cuts at: 2478 2713 3090 3100 3177 3216 3382 3400 3453
Size: 235 377 10 77 39 166 18 53
Cuts at: 3453 3519 5492 5988 2478
Size: 66 1973 496 2779
Fragments arranged by size:
2779 1973 496 377 235 166 77 66
53 39 18 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2795 2951 3139 1163
Size: 573 1059 156 188 4313
Fragments arranged by size:
4313 1059 573 188 156
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3739 4500 5528 1540
Size: 275 186 1738 761 1028 2301
Fragments arranged by size:
2301 1738 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2129 2664 3140 3240 4350 4435 5506 2129
Size: 535 476 100 1110 85 1071 2912
Fragments arranged by size:
2912 1110 1071 535 476 100 85
BsmI GAATG_Cn'
Cuts at: 2062 2912 3800 3893 2062
Size: 850 888 93 4458
Fragments arranged by size:
4458 888 850 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2129 2298 2479 2496 2621 2664
Size: 399 233 106 169 181 17 125 43
Cuts at: 2664 2909 2934 3100 3140 3230 3240 3608 4350
Size: 245 25 166 40 90 10 368 742
Cuts at: 4350 4435 5506 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 368 245 233 181
169 166 125 106 90 85 43 40
25 17 10
BspEI T'CCGG_A
Cuts at: 5952 5952
Size: 6289
BspHI T'CATG_A
Cuts at: 4178 5908 4178
Size: 1730 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2098 2871 3644 844
Size: 1254 773 773 3489
Fragments arranged by size:
3489 1254 773 773
BsrBI CCG'CTC
Cuts at: 1394 1823 3334 3531 4176 1394
Size: 429 1511 197 645 3507
Fragments arranged by size:
3507 1511 645 429 197
BsrDI GCAATG_nn'
Cuts at: 65 1248 2925 4784 4958 65
Size: 1183 1677 1859 174 1396
Fragments arranged by size:
1859 1677 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4933 5120 1700
Size: 110 3123 187 2869
Fragments arranged by size:
3123 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6289
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6289
BssSI C'ACGA_G
Cuts at: 2762 4349 5361 2762
Size: 1587 1012 3690
Fragments arranged by size:
3690 1587 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2335 794
Size: 1541 4748
BstBI TT'CG_AA
Cuts at: 5093 5093
Size: 6289
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6289
BstYI r'GATC_y
Cuts at: 1972 2512 3619 3986 4371 4388 5840 5926 5938
Size: 540 1107 367 385 17 1452 86 12
Cuts at: 5938 6284 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1107 540 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3145 3145
Size: 6289
Bsu36I CC'TnA_GG
Cuts at: 2507 2622 2507
Size: 115 6174
BtgI C'CryG_G
Cuts at: 513 1065 2004 3231 513
Size: 552 939 1227 3571
Fragments arranged by size:
3571 1227 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2875 487
Size: 683 56 1649 3901
Fragments arranged by size:
3901 1649 683 56
BtsI GCAGTG_nn'
Cuts at: 3805 4599 4619 3805
Size: 794 20 5475
Fragments arranged by size:
5475 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6254
DraI TTT'AAA
Cuts at: 3593 3593
Size: 6289
DraIII CAC_nnn'GTG
Cuts at: 3350 3350
Size: 6289
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5296 809
Size: 507 3980 1802
Fragments arranged by size:
3980 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2955 3000 3736
Size: 54 1425 50 275 18 1125 45 736
Cuts at: 3736 4625 6254 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1125 889 736 275 54 50
45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3736 1537
Size: 275 1924 4090
Fragments arranged by size:
4090 1924 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3284 3470 4219 1353
Size: 1931 186 749 3423
Fragments arranged by size:
3423 1931 749 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3353 4876 5250 5396 387
Size: 1064 138 1764 1523 374 146 1280
Fragments arranged by size:
1764 1523 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2127 3138 2127
Size: 1011 5278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2306 2635 3550 4352 4949 5736
Size: 393 548 126 329 915 802 597 787
Cuts at: 5736 6004 1239
Size: 268 1524
Fragments arranged by size:
1524 915 802 787 597 548 393 329
268 126
EcoO109I rG'GnC_Cy
Cuts at: 2475 3096 3097 3357 2475
Size: 621 1 260 5407
Fragments arranged by size:
5407 621 260 1
EcoRI G'AATT_C
Cuts at: 3598 3976 3598
Size: 378 5911
FspI TGC'GCA
Cuts at: 3320 4795 3320
Size: 1475 4814
HaeII r_GCGC'y
Cuts at: 1678 3308 5436 1678
Size: 1630 2128 2531
Fragments arranged by size:
2531 2128 1630
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4930 4962 5004 5036 1373
Size: 32 135 32 3358 32 42 32 2626
Fragments arranged by size:
3358 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2147 3633 3879 669
Size: 988 281 209 1486 246 3079
Fragments arranged by size:
3079 1486 988 281 246 209
HindIII A'AGCT_T
Cuts at: 748 3074 748
Size: 2326 3963
HpaI GTT'AAC
Cuts at: 3879 3879
Size: 6289
KpnI G_GTAC'C
Cuts at: 3614 3614
Size: 6289
MfeI C'AATT_G
Cuts at: 3888 3888
Size: 6289
MluI A'CGCG_T
Cuts at: 6165 6165
Size: 6289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2439 5403 5587 1305
Size: 1134 2964 184 2007
Fragments arranged by size:
2964 2007 1134 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3002 10
Size: 54 1425 343 1170 3297
Fragments arranged by size:
3297 1425 1170 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2016 2855 2996 4247 4606 4765 518
Size: 648 850 839 141 1251 359 159 2042
Fragments arranged by size:
2042 1251 850 839 648 359 159 141
MspA1I CmG'CkG
Cuts at: 1606 2158 2482 2670 2960 2985 3177 3457 3523
Size: 552 324 188 290 25 192 280 66
Cuts at: 3523 3705 4382 5530 5775 1606
Size: 182 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 552 324 290 280 245
192 188 182 66 25
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4798
Fragments arranged by size:
4798 939 552
NdeI CA'TA_TG
Cuts at: 387 3212 387
Size: 2825 3464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6289
NotI GC'GGCC_GC
Cuts at: 3736 3736
Size: 6289
NspI r_CATG'y
Cuts at: 1132 1229 2318 2870 2946 3208 3647 5192 1132
Size: 97 1089 552 76 262 439 1545 2229
Fragments arranged by size:
2229 1545 1089 552 439 262 97 76
PasI CC'CwG_GG
Cuts at: 1348 2382 2604 1348
Size: 1034 222 5033
Fragments arranged by size:
5033 1034 222
PciI A'CATG_T
Cuts at: 3204 5188 3204
Size: 1984 4305
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2158 2230 2548 3216 1108
Size: 1050 72 318 668 4181
Fragments arranged by size:
4181 1050 668 318 72
PmeI GTTT'AAAC
Cuts at: 3593 3593
Size: 6289
PmlI CAC'GTG
Cuts at: 2497 2497
Size: 6289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4358 4390 5871 5903 4358
Size: 32 1481 32 4744
Fragments arranged by size:
4744 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2570 1613
Size: 957 5332
PsiI TTA'TAA
Cuts at: 3859 3859
Size: 6289
PspOMI G'GGCC_C
Cuts at: 2475 3096 2475
Size: 621 5668
PstI C_TGCA'G
Cuts at: 830 2731 2774 3641 830
Size: 1901 43 867 3478
Fragments arranged by size:
3478 1901 867 43
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6289
PvuII CAG'CTG
Cuts at: 2158 2482 2985 3177 3457 2158
Size: 324 503 192 280 4990
Fragments arranged by size:
4990 503 324 280 192
SacI G_AGCT'C
Cuts at: 2129 3140 2129
Size: 1011 5278
SalI G'TCGA_C
Cuts at: 1936 3631 1936
Size: 1695 4594
SbfI CC_TGCA'GG
Cuts at: 3641 3641
Size: 6289
ScaI AGT'ACT
Cuts at: 4537 6249 4537
Size: 1712 4577
SexAI A'CCwGG_T
Cuts at: 3285 3285
Size: 6289
SfcI C'TryA_G
Cuts at: 826 1045 2727 2770 3637 4772 5453 5644 826
Size: 219 1682 43 867 1135 681 191 1471
Fragments arranged by size:
1682 1471 1135 867 681 219 191 43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6289
SmaI CCC'GGG
Cuts at: 1872 2321 2360 2935 3616 1872
Size: 449 39 575 681 4545
Fragments arranged by size:
4545 681 575 449 39
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4393 5294 5556 820
Size: 197 926 2450 901 262 1553
Fragments arranged by size:
2450 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2751 4080 493
Size: 2258 1329 2702
Fragments arranged by size:
2702 2258 1329
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6289
SphI G_CATG'C
Cuts at: 1132 2318 2870 2946 3647 1132
Size: 1186 552 76 701 3774
Fragments arranged by size:
3774 1186 701 552 76
SspI AAT'ATT
Cuts at: 5 52 4213 5
Size: 47 4161 2081
Fragments arranged by size:
4161 2081 47
StuI AGG'CCT
Cuts at: 1517 3563 1517
Size: 2046 4243
StyI C'CwwG_G
Cuts at: 513 1065 2004 3417 3546 3728 513
Size: 552 939 1413 129 182 3074
Fragments arranged by size:
3074 1413 939 552 182 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2230 2435 3208 4316 4333 4486 4671
Size: 1255 650 205 773 1108 17 153 185
Cuts at: 4671 5116 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1108 773 650 445 205 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4535 6247 96
Size: 274 80 33 51 1443 2558 1712 138
Fragments arranged by size:
2558 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2205 2237 2818 2850 6249 6281 2205
Size: 32 581 32 3399 32 2213
Fragments arranged by size:
3399 2213 581 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3371 1316
Size: 2055 4234
XbaI T'CTAG_A
Cuts at: 3625 3625
Size: 6289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2227 2486 3415 2227
Size: 259 929 5101
Fragments arranged by size:
5101 929 259
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6289
XmaI C'CCGG_G
Cuts at: 1870 2319 2358 2933 3614 1870
Size: 449 39 575 681 4545
Fragments arranged by size:
4545 681 575 449 39
XmnI GAAnn'nnTTC
Cuts at: 4418 4418
Size: 6289
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4097 276
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI