Restriction map for
pFN21AE9453
(Circular) (Six-base) MAPSORT of: pFN21AE9453.seq Check: 5489 from: 1 to: 7435
pFN21AE9453 7435 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5245 278
Size: 53 83 186 936 3709 2468
Fragments arranged by size:
3709 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4778 1937
Size: 2841 4594
Acc65I G'GTAC_C
Cuts at: 4756 4756
Size: 7435
AclI AA'CG_TT
Cuts at: 1563 5562 5935 1563
Size: 3999 373 3063
Fragments arranged by size:
3999 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 2231 5498 6882 7150 1632
Size: 512 87 3267 1384 268 1917
Fragments arranged by size:
3267 1917 1384 512 268 87
AflII C'TTAA_G
Cuts at: 820 1017 4570 820
Size: 197 3553 3685
Fragments arranged by size:
3685 3553 197
AflIII A'CryG_T
Cuts at: 1203 2689 3869 6334 7311 1203
Size: 1486 1180 2465 977 1327
Fragments arranged by size:
2465 1486 1327 1180 977
AgeI A'CCGG_T
Cuts at: 6266 6266
Size: 7435
AhdI GACnn_n'nnGTC
Cuts at: 6164 6164
Size: 7435
AleI CACnn'nnGTG
Cuts at: 1166 3245 3773 3953 1166
Size: 2079 528 180 4648
Fragments arranged by size:
4648 2079 528 180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3263 1184
Size: 2079 5356
AlwNI CAG_nnn'CTG
Cuts at: 2155 3983 4453 5178 6750 2155
Size: 1828 470 725 1572 2840
Fragments arranged by size:
2840 1828 1572 725 470
ApaLI G'TGCA_C
Cuts at: 5492 6648 5492
Size: 1156 6279
ApoI r'AATT_y
Cuts at: 1493 2203 2506 2550 2577 2932 3271 3284 4628
Size: 710 303 44 27 355 339 13 1344
Cuts at: 4628 4744 4970 5122 7231 1493
Size: 116 226 152 2109 1697
Fragments arranged by size:
2109 1697 1344 710 355 339 303 226
152 116 44 27 13
AseI AT'TA_AT
Cuts at: 160 1032 2478 5989 160
Size: 872 1446 3511 1606
Fragments arranged by size:
3511 1606 1446 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7435
AvaI C'yCGr_G
Cuts at: 1870 1943 4760 1870
Size: 73 2817 4545
Fragments arranged by size:
4545 2817 73
BamHI G'GATC_C
Cuts at: 4765 5132 4765
Size: 367 7068
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3247 3935 4128 4756 6211
Size: 325 217 627 1460 688 193 628 1455
Cuts at: 6211 618
Size: 1842
Fragments arranged by size:
1842 1460 1455 688 628 627 325 217
193
BanII G_rGCy'C
Cuts at: 1391 4410 4754 1391
Size: 3019 344 4072
Fragments arranged by size:
4072 3019 344
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2328 2745 928
Size: 971 429 417 5618
Fragments arranged by size:
5618 971 429 417
BbvCI CC'TCA_GC
Cuts at: 2423 2423
Size: 7435
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5626 5660 1903
Size: 34 3689 34 3678
Fragments arranged by size:
3689 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5327 6537 5327
Size: 1210 6225
BclI T'GATC_A
Cuts at: 1548 3816 1548
Size: 2268 5167
BfrBI ATG'CAT
Cuts at: 4487 4673 4487
Size: 186 7249
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2061 2702 2963
Size: 107 122 71 631 605 389 641 261
Cuts at: 2963 6046 136
Size: 3083 1525
Fragments arranged by size:
3083 1525 641 631 605 389 261 122
107 71
BglII A'GATC_T
Cuts at: 7430 7430
Size: 7435
BlpI GC'TnA_GC
Cuts at: 4852 4852
Size: 7435
Bme1580I G_kGCm'C
Cuts at: 1790 2468 4133 5496 6652 1790
Size: 678 1665 1363 1156 2573
Fragments arranged by size:
2573 1665 1363 1156 678
BmgBI CAC'GTC
Cuts at: 1318 3684 3872 1318
Size: 2366 188 4881
Fragments arranged by size:
4881 2366 188
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2129 2764 3791
Size: 416 53 83 399 24 712 635 1027
Cuts at: 3791 5166 6124 442
Size: 1375 958 1753
Fragments arranged by size:
1753 1375 1027 958 712 635 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7435
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3615 3615
Size: 7435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2406 2638 3987 4333 4707 6095 1239
Size: 1167 232 1349 346 374 1388 2579
Fragments arranged by size:
2579 1388 1349 1167 374 346 232
Bpu10I CC'TnA_GC
Cuts at: 2423 3501 3590 2423
Size: 1078 89 6268
Fragments arranged by size:
6268 1078 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3654 5560 6425 6723 3654
Size: 1906 865 298 4366
Fragments arranged by size:
4366 1906 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6098 882
Size: 617 4599 2219
Fragments arranged by size:
4599 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5226 493
Size: 711 4022 2702
Fragments arranged by size:
4022 2702 711
BsaBI GATnn'nnATC
Cuts at: 7429 7429
Size: 7435
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5242 5624 6259 275
Size: 53 83 186 936 3709 382 635 1451
Fragments arranged by size:
3709 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4056 5868 6266 6540 6687 7098 4056
Size: 1812 398 274 147 411 4393
Fragments arranged by size:
4393 1812 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3689 3719 1178
Size: 30 165 30 2286 30 4894
Fragments arranged by size:
4894 2286 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4373 1186
Size: 3187 4248
BseYI C'CCAG_C
Cuts at: 2633 4086 6638 7134 2633
Size: 1453 2552 496 2934
Fragments arranged by size:
2934 2552 1453 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3958 4239 1163
Size: 573 2222 281 4359
Fragments arranged by size:
4359 2222 573 281
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4885 5646 6674 1540
Size: 275 186 2884 761 1028 2301
Fragments arranged by size:
2884 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2392 2907 5496 5581 6652 2392
Size: 515 2589 85 1071 3175
Fragments arranged by size:
3175 2589 1071 515 85
BsmI GAATG_Cn'
Cuts at: 4487 4946 5039 4487
Size: 459 93 6883
Fragments arranged by size:
6883 459 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2392 2468 2907 4133 4410 4754 5496
Size: 399 602 76 439 1226 277 344 742
Cuts at: 5496 5581 6652 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1226 1071 742 602 439 399 344
277 85 76
BspEI T'CCGG_A
Cuts at: 7098 7098
Size: 7435
BspHI T'CATG_A
Cuts at: 5324 7054 5324
Size: 1730 5705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2872 4790 844
Size: 2028 1918 3489
Fragments arranged by size:
3489 2028 1918
BsrBI CCG'CTC
Cuts at: 1394 1823 5322 1394
Size: 429 3499 3507
Fragments arranged by size:
3507 3499 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2959 4155 5930 6104 65
Size: 1183 1711 1196 1775 174 1396
Fragments arranged by size:
1775 1711 1396 1196 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6079 6266 1700
Size: 110 4269 187 2869
Fragments arranged by size:
4269 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7435
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7435
BssSI C'ACGA_G
Cuts at: 4067 5495 6507 4067
Size: 1428 1012 4995
Fragments arranged by size:
4995 1428 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7435
BstBI TT'CG_AA
Cuts at: 2614 6239 2614
Size: 3625 3810
BstEII G'GTnAC_C
Cuts at: 1184 2164 2602 1184
Size: 980 438 6017
Fragments arranged by size:
6017 980 438
BstXI CCAn_nnnn'nTGG
Cuts at: 2235 2235
Size: 7435
BstYI r'GATC_y
Cuts at: 1972 3422 4765 5132 5517 5534 6986 7072 7084
Size: 1450 1343 367 385 17 1452 86 12
Cuts at: 7084 7430 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1450 1343 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2056 3525 3951 513
Size: 552 991 1469 426 3997
Fragments arranged by size:
3997 1469 991 552 426
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6696
Fragments arranged by size:
6696 683 56
BtsI GCAGTG_nn'
Cuts at: 2315 2665 2839 3638 4649 4951 5745 5765 2315
Size: 350 174 799 1011 302 794 20 3985
Fragments arranged by size:
3985 1011 799 794 350 302 174 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7435
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3009 3044 529
Size: 35 2445 35 4920
Fragments arranged by size:
4920 2445 35 35
DraI TTT'AAA
Cuts at: 4739 4739
Size: 7435
DraIII CAC_nnn'GTG
Cuts at: 3658 3658
Size: 7435
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6442 809
Size: 507 5126 1802
Fragments arranged by size:
5126 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 3084 4882
Size: 54 1425 50 275 18 223 1031 1798
Cuts at: 4882 5771 7400 8
Size: 889 1629 43
Fragments arranged by size:
1798 1629 1425 1031 889 275 223 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4882 1537
Size: 275 3070 4090
Fragments arranged by size:
4090 3070 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3267 4370 5365 1353
Size: 1914 1103 995 3423
Fragments arranged by size:
3423 1914 1103 995
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6022 6396 6542 387
Size: 1064 138 4433 374 146 1280
Fragments arranged by size:
4433 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2231 2406 2638 3987 4333 4707
Size: 393 512 87 175 232 1349 346 374
Cuts at: 4707 5498 6095 6882 7150 1239
Size: 791 597 787 268 1524
Fragments arranged by size:
1524 1349 791 787 597 512 393 374
346 268 232 175 87
EcoNI CCTnn'n_nnAGG
Cuts at: 4186 4401 4186
Size: 215 7220
EcoRI G'AATT_C
Cuts at: 2506 2577 4744 5122 2506
Size: 71 2167 378 4819
Fragments arranged by size:
4819 2167 378 71
EcoRV GAT'ATC
Cuts at: 3522 3522
Size: 7435
FspI TGC'GCA
Cuts at: 5941 5941
Size: 7435
HaeII r_GCGC'y
Cuts at: 1678 6582 1678
Size: 4904 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3771 3803 4488 4520 6076
Size: 32 135 32 2199 32 685 32 1556
Cuts at: 6076 6108 6150 6182 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2199 1556 685 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3459 4779 5025 669
Size: 988 281 1521 1320 246 3079
Fragments arranged by size:
3079 1521 1320 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7435
HpaI GTT'AAC
Cuts at: 5025 5025
Size: 7435
KpnI G_GTAC'C
Cuts at: 4760 4760
Size: 7435
MfeI C'AATT_G
Cuts at: 5034 5034
Size: 7435
MluI A'CGCG_T
Cuts at: 7311 7311
Size: 7435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1993 3278 4087 4722 6549 6733 1305
Size: 688 1285 809 635 1827 184 2007
Fragments arranged by size:
2007 1827 1285 809 688 635 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3086 10
Size: 54 1425 343 1254 4359
Fragments arranged by size:
4359 1425 1254 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2233 3245 3719 3773 3953 4067 4367
Size: 648 1067 1012 474 54 180 114 300
Cuts at: 4367 5393 5752 5911 518
Size: 1026 359 159 2042
Fragments arranged by size:
2042 1067 1026 1012 648 474 359 300
180 159 114 54
MspA1I CmG'CkG
Cuts at: 1606 2152 2969 3980 4851 5528 6676 6921 1606
Size: 546 817 1011 871 677 1148 245 2120
Fragments arranged by size:
2120 1148 1011 871 817 677 546 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6883
NdeI CA'TA_TG
Cuts at: 387 2569 387
Size: 2182 5253
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7435
NotI GC'GGCC_GC
Cuts at: 4882 4882
Size: 7435
NsiI A_TGCA'T
Cuts at: 4489 4675 4489
Size: 186 7249
NspI r_CATG'y
Cuts at: 1132 1229 2693 4466 4793 6338 1132
Size: 97 1464 1773 327 1545 2229
Fragments arranged by size:
2229 1773 1545 1464 327 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7435
PciI A'CATG_T
Cuts at: 2689 6334 2689
Size: 3645 3790
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4081 4453 1108
Size: 2973 372 4090
Fragments arranged by size:
4090 2973 372
PmeI GTTT'AAAC
Cuts at: 4739 4739
Size: 7435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5504 5536 7017 7049 5504
Size: 32 1481 32 5890
Fragments arranged by size:
5890 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7435
PsiI TTA'TAA
Cuts at: 5005 5005
Size: 7435
PstI C_TGCA'G
Cuts at: 830 2310 2831 4787 830
Size: 1480 521 1956 3478
Fragments arranged by size:
3478 1956 1480 521
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7435
PvuII CAG'CTG
Cuts at: 2152 2969 3980 2152
Size: 817 1011 5607
Fragments arranged by size:
5607 1011 817
SalI G'TCGA_C
Cuts at: 1936 4777 1936
Size: 2841 4594
SapI GCTCTTCn'nnn_
Cuts at: 3267 4370 3267
Size: 1103 6332
SbfI CC_TGCA'GG
Cuts at: 4787 4787
Size: 7435
ScaI AGT'ACT
Cuts at: 5683 7395 5683
Size: 1712 5723
SexAI A'CCwGG_T
Cuts at: 2333 2333
Size: 7435
SfcI C'TryA_G
Cuts at: 826 1045 2170 2306 2827 2839 4783 5918 6599
Size: 219 1125 136 521 12 1944 1135 681
Cuts at: 6599 6790 826
Size: 191 1471
Fragments arranged by size:
1944 1471 1135 1125 681 521 219 191
136 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7435
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7435
SmaI CCC'GGG
Cuts at: 1872 4762 1872
Size: 2890 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3669 4570 5539 6440 6702 820
Size: 197 926 1726 901 969 901 262 1553
Fragments arranged by size:
1726 1553 969 926 901 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5226 493
Size: 4733 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7435
SphI G_CATG'C
Cuts at: 1132 4793 1132
Size: 3661 3774
SspI AAT'ATT
Cuts at: 5 52 3067 5359 5
Size: 47 3015 2292 2081
Fragments arranged by size:
3015 2292 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7435
StyI C'CwwG_G
Cuts at: 513 1065 2181 2802 4874 513
Size: 552 1116 621 2072 3074
Fragments arranged by size:
3074 2072 1116 621 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2621 2865 4116 5462 5479 5632 5817
Size: 1255 1041 244 1251 1346 17 153 185
Cuts at: 5817 6262 325
Size: 445 1498
Fragments arranged by size:
1498 1346 1255 1251 1041 445 244 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3692 5681 7393
Size: 274 80 33 51 1443 1715 1989 1712
Cuts at: 7393 96
Size: 138
Fragments arranged by size:
1989 1715 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2323 2355 3689 3717 3721 3749 4422 4454 7395
Size: 32 1334 28 4 28 673 32 2941
Cuts at: 7395 7427 2323
Size: 32 2331
Fragments arranged by size:
2941 2331 1334 673 32 32 32 28
28 4
Tth111I GACn'n_nGTC
Cuts at: 1316 2783 4338 1316
Size: 1467 1555 4413
Fragments arranged by size:
4413 1555 1467
XbaI T'CTAG_A
Cuts at: 4771 4771
Size: 7435
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2615 3112 4366 2615
Size: 497 1254 5684
Fragments arranged by size:
5684 1254 497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7435
XmaI C'CCGG_G
Cuts at: 1870 4760 1870
Size: 2890 4545
XmnI GAAnn'nnTTC
Cuts at: 2577 5564 2577
Size: 2987 4448
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5243 276
Size: 53 83 186 936 3709 2468
Fragments arranged by size:
3709 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI