Restriction map for
pFN21AE9451
(Circular) (Six-base) MAPSORT of: pFN21AE9451.seq Check: 9757 from: 1 to: 7168
pFN21AE9451 7168 bp
With 182 enzymes: SgfI *
March 28, 2012 12:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2747 4265 2747
Size: 1518 5650
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3200 4978 278
Size: 53 83 186 936 1664 1778 2468
Fragments arranged by size:
2468 1778 1664 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4511 1937
Size: 2574 4594
Acc65I G'GTAC_C
Cuts at: 4489 4489
Size: 7168
AclI AA'CG_TT
Cuts at: 1563 5295 5668 1563
Size: 3732 373 3063
Fragments arranged by size:
3732 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2926 3058 3143 3551 3901 3920 5231 6615
Size: 1294 132 85 408 350 19 1311 1384
Cuts at: 6615 6883 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1311 1294 408 350 268 132
85 19
AfeI AGC'GCT
Cuts at: 3482 3482
Size: 7168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6971
AflIII A'CryG_T
Cuts at: 1203 6067 7044 1203
Size: 4864 977 1327
Fragments arranged by size:
4864 1327 977
AgeI A'CCGG_T
Cuts at: 5999 5999
Size: 7168
AhdI GACnn_n'nnGTC
Cuts at: 5897 5897
Size: 7168
AleI CACnn'nnGTG
Cuts at: 1166 3434 3671 3948 1166
Size: 2268 237 277 4386
Fragments arranged by size:
4386 2268 277 237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3966 1184
Size: 2782 4386
AlwNI CAG_nnn'CTG
Cuts at: 2427 4911 6483 2427
Size: 2484 1572 3112
Fragments arranged by size:
3112 2484 1572
ApaLI G'TGCA_C
Cuts at: 3054 5225 6381 3054
Size: 2171 1156 3841
Fragments arranged by size:
3841 2171 1156
ApoI r'AATT_y
Cuts at: 1493 2776 3179 3538 4477 4703 4855 6964 1493
Size: 1283 403 359 939 226 152 2109 1697
Fragments arranged by size:
2109 1697 1283 939 403 359 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5722 160
Size: 872 4690 1606
Fragments arranged by size:
4690 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7168
AvaI C'yCGr_G
Cuts at: 1870 1943 3543 4493 1870
Size: 73 1600 950 4545
Fragments arranged by size:
4545 1600 950 73
BamHI G'GATC_C
Cuts at: 2221 4498 4865 2221
Size: 2277 367 4524
Fragments arranged by size:
4524 2277 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4489 5944 618
Size: 325 217 627 2702 1455 1842
Fragments arranged by size:
2702 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2876 3792 4487 1391
Size: 1485 916 695 4072
Fragments arranged by size:
4072 1485 916 695
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2523 3208 4400 928
Size: 971 624 685 1192 3696
Fragments arranged by size:
3696 1192 971 685 624
BbvCI CC'TCA_GC
Cuts at: 3265 3265
Size: 7168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5359 5393 1903
Size: 34 50 34 3338 34 3678
Fragments arranged by size:
3678 3338 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4125 5060 6270 4125
Size: 935 1210 5023
Fragments arranged by size:
5023 1210 935
BclI T'GATC_A
Cuts at: 1548 4098 1548
Size: 2550 4618
BfrBI ATG'CAT
Cuts at: 4024 4024
Size: 7168
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2748 5779 136
Size: 107 122 71 631 605 1076 3031 1525
Fragments arranged by size:
3031 1525 1076 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7163 7163
Size: 7168
BlpI GC'TnA_GC
Cuts at: 4585 4585
Size: 7168
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2129 2693 3058 5229 6385 1790
Size: 277 62 564 365 2171 1156 2573
Fragments arranged by size:
2573 2171 1156 564 365 277 62
BmgBI CAC'GTC
Cuts at: 1318 2521 1318
Size: 1203 5965
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2371 4398 4405
Size: 416 53 83 399 24 954 2027 7
Cuts at: 4405 4899 5857 442
Size: 494 958 1753
Fragments arranged by size:
2027 1753 958 954 494 416 399 83
53 24 7
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7168
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3217 3217
Size: 7168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3242 3588 3632 3682 4084 5828 1239
Size: 2003 346 44 50 402 1744 2579
Fragments arranged by size:
2579 2003 1744 402 346 50 44
Bpu10I CC'TnA_GC
Cuts at: 2433 3265 2433
Size: 832 6336
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2653 4408 5293 6158 6456 2653
Size: 1755 885 865 298 3365
Fragments arranged by size:
3365 1755 885 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2651 3419 5831 882
Size: 617 1152 768 2412 2219
Fragments arranged by size:
2412 2219 1152 768 617
BsaAI yAC'GTr
Cuts at: 493 1204 3171 4959 493
Size: 711 1967 1788 2702
Fragments arranged by size:
2702 1967 1788 711
BsaBI GATnn'nnATC
Cuts at: 2614 3386 3691 7162 2614
Size: 772 305 3471 2620
Fragments arranged by size:
3471 2620 772 305
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3197 3779 4975 5357
Size: 53 83 186 936 1664 582 1196 382
Cuts at: 5357 5992 275
Size: 635 1451
Fragments arranged by size:
1664 1451 1196 936 635 582 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5601 5999 6273 6420 6831 5601
Size: 398 274 147 411 5938
Fragments arranged by size:
5938 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2701 2731 1178
Size: 30 165 30 1298 30 5615
Fragments arranged by size:
5615 1298 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 1186
Size: 829 6339
BseYI C'CCAG_C
Cuts at: 2055 2128 2268 4215 4246 4403 6371 6867 2055
Size: 73 140 1947 31 157 1968 496 2356
Fragments arranged by size:
2356 1968 1947 496 157 140 73 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2341 2553 2842 3809 3926 4022 1163
Size: 573 605 212 289 967 117 96 4309
Fragments arranged by size:
4309 967 605 573 289 212 117 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3111 4618 5379 6407 1540
Size: 275 186 1110 1507 761 1028 2301
Fragments arranged by size:
2301 1507 1110 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3058 3668 3792 5229 5314 6385 3058
Size: 610 124 1437 85 1071 3841
Fragments arranged by size:
3841 1437 1071 610 124 85
BsmI GAATG_Cn'
Cuts at: 2437 2681 3338 4026 4679 4772 2437
Size: 244 657 688 653 93 4833
Fragments arranged by size:
4833 688 657 653 244 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3188 3188
Size: 7168
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2129 2693 2876 3058 3668 3792
Size: 399 277 62 564 183 182 610 124
Cuts at: 3792 4487 5229 5314 6385 1391
Size: 695 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 695 610 564 399 277
183 182 124 85 62
BspEI T'CCGG_A
Cuts at: 6831 6831
Size: 7168
BspHI T'CATG_A
Cuts at: 5057 6787 5057
Size: 1730 5438
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2028 2747 3043 4265 4523 844
Size: 1184 719 296 1222 258 3489
Fragments arranged by size:
3489 1222 1184 719 296 258
BsrBI CCG'CTC
Cuts at: 1394 1823 5055 1394
Size: 429 3232 3507
Fragments arranged by size:
3507 3232 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2206 2770 2892 3014 5663 5837 65
Size: 1183 958 564 122 122 2649 174 1396
Fragments arranged by size:
2649 1396 1183 958 564 174 122 122
BsrFI r'CCGG_y
Cuts at: 1700 1810 5812 5999 1700
Size: 110 4002 187 2869
Fragments arranged by size:
4002 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7168
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7168
BssSI C'ACGA_G
Cuts at: 5228 6240 5228
Size: 1012 6156
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2427 3764 794
Size: 1633 1337 4198
Fragments arranged by size:
4198 1633 1337
BstBI TT'CG_AA
Cuts at: 5972 5972
Size: 7168
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7168
BstXI CCAn_nnnn'nTGG
Cuts at: 2155 2276 2869 2933 3436 3775 2155
Size: 121 593 64 503 339 5548
Fragments arranged by size:
5548 593 503 339 121 64
BstYI r'GATC_y
Cuts at: 1972 2221 2536 2827 3000 3592 4428 4498 4865
Size: 249 315 291 173 592 836 70 367
Cuts at: 4865 5250 5267 6719 6805 6817 7163 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 836 592 385 367 346 315
291 249 173 86 70 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2457 513
Size: 552 1392 5224
Fragments arranged by size:
5224 1392 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2248 3796 487
Size: 683 56 1022 1548 3859
Fragments arranged by size:
3859 1548 1022 683 56
BtsI GCAGTG_nn'
Cuts at: 2629 3033 3343 4684 5478 5498 2629
Size: 404 310 1341 794 20 4299
Fragments arranged by size:
4299 1341 794 404 310 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7133
DraI TTT'AAA
Cuts at: 3474 4472 3474
Size: 998 6170
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6175 809
Size: 507 4859 1802
Fragments arranged by size:
4859 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2626 3207 4354
Size: 54 1425 50 275 18 796 581 1147
Cuts at: 4354 4615 5504 7133 8
Size: 261 889 1629 43
Fragments arranged by size:
1629 1425 1147 889 796 581 275 261
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4615 1537
Size: 275 2803 4090
Fragments arranged by size:
4090 2803 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 2347 2966 3186 3336 4226 5098 1353
Size: 680 314 619 220 150 890 872 3423
Fragments arranged by size:
3423 890 872 680 619 314 220 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5755 6129 6275 387
Size: 1064 138 4166 374 146 1280
Fragments arranged by size:
4166 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3790 3790
Size: 7168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2926 3058 3143 3242 3551 3588 3632
Size: 393 1294 132 85 99 309 37 44
Cuts at: 3632 3682 3901 3920 4084 5231 5828 6615 6883
Size: 50 219 19 164 1147 597 787 268
Cuts at: 6883 1239
Size: 1524
Fragments arranged by size:
1524 1294 1147 787 597 393 309 268
219 164 132 99 85 50 44 37
19
EcoNI CCTnn'n_nnAGG
Cuts at: 4272 4272
Size: 7168
EcoO109I rG'GnC_Cy
Cuts at: 2365 4320 2365
Size: 1955 5213
EcoRI G'AATT_C
Cuts at: 3538 4477 4855 3538
Size: 939 378 5851
Fragments arranged by size:
5851 939 378
EcoRV GAT'ATC
Cuts at: 2401 2401
Size: 7168
FspI TGC'GCA
Cuts at: 5674 5674
Size: 7168
HaeII r_GCGC'y
Cuts at: 1678 3484 6315 1678
Size: 1806 2831 2531
Fragments arranged by size:
2831 2531 1806
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2324 2356 3526 3558 3572
Size: 32 135 32 752 32 1170 32 14
Cuts at: 3572 3604 3745 3777 5809 5841 5883 5915 1373
Size: 32 141 32 2032 32 42 32 2626
Fragments arranged by size:
2626 2032 1170 752 141 135 42 32
32 32 32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2452 4512 4758 669
Size: 988 281 514 2060 246 3079
Fragments arranged by size:
3079 2060 988 514 281 246
HindIII A'AGCT_T
Cuts at: 748 3717 748
Size: 2969 4199
HpaI GTT'AAC
Cuts at: 4758 4758
Size: 7168
KpnI G_GTAC'C
Cuts at: 4493 4493
Size: 7168
MfeI C'AATT_G
Cuts at: 3402 4767 3402
Size: 1365 5803
MluI A'CGCG_T
Cuts at: 7044 7044
Size: 7168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2467 2560 3336 6282 6466 1305
Size: 1162 93 776 2946 184 2007
Fragments arranged by size:
2946 2007 1162 776 184 93
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2628 3209 10
Size: 54 1425 343 796 581 3969
Fragments arranged by size:
3969 1425 796 581 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2684 3434 3671 3773 3935 3948 5126
Size: 648 1518 750 237 102 162 13 1178
Cuts at: 5126 5485 5644 518
Size: 359 159 2042
Fragments arranged by size:
2042 1518 1178 750 648 359 237 162
159 102 13
MspA1I CmG'CkG
Cuts at: 1606 2055 2325 2478 2883 2946 3744 4584 5261
Size: 449 270 153 405 63 798 840 677
Cuts at: 5261 6409 6654 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 840 798 677 449 405 270
245 153 63
NcoI C'CATG_G
Cuts at: 513 1065 2457 513
Size: 552 1392 5224
Fragments arranged by size:
5224 1392 552
NdeI CA'TA_TG
Cuts at: 387 3040 387
Size: 2653 4515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7168
NotI GC'GGCC_GC
Cuts at: 4615 4615
Size: 7168
NsiI A_TGCA'T
Cuts at: 4026 4026
Size: 7168
NspI r_CATG'y
Cuts at: 1132 1229 2561 2709 4526 6071 1132
Size: 97 1332 148 1817 1545 2229
Fragments arranged by size:
2229 1817 1545 1332 148 97
PasI CC'CwG_GG
Cuts at: 1348 2869 1348
Size: 1521 5647
PciI A'CATG_T
Cuts at: 6067 6067
Size: 7168
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2765 2794 1108
Size: 1657 29 5482
Fragments arranged by size:
5482 1657 29
PfoI T'CCnGG_A
Cuts at: 2224 3219 3659 3711 2224
Size: 995 440 52 5681
Fragments arranged by size:
5681 995 440 52
PmeI GTTT'AAAC
Cuts at: 4472 4472
Size: 7168
PmlI CAC'GTG
Cuts at: 3171 3171
Size: 7168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5237 5269 6750 6782 4040
Size: 32 1165 32 1481 32 4426
Fragments arranged by size:
4426 1481 1165 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2365 2365
Size: 7168
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7168
PsiI TTA'TAA
Cuts at: 4738 4738
Size: 7168
PstI C_TGCA'G
Cuts at: 830 3743 4317 4520 830
Size: 2913 574 203 3478
Fragments arranged by size:
3478 2913 574 203
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7168
PvuII CAG'CTG
Cuts at: 2055 2325 2478 2883 2946 3744 2055
Size: 270 153 405 63 798 5479
Fragments arranged by size:
5479 798 405 270 153 63
SacI G_AGCT'C
Cuts at: 3792 3792
Size: 7168
SalI G'TCGA_C
Cuts at: 1936 4510 1936
Size: 2574 4594
SanDI GG'GwC_CC
Cuts at: 2365 2365
Size: 7168
SapI GCTCTTCn'nnn_
Cuts at: 4226 4226
Size: 7168
SbfI CC_TGCA'GG
Cuts at: 4317 4520 4317
Size: 203 6965
ScaI AGT'ACT
Cuts at: 5416 7128 5416
Size: 1712 5456
SexAI A'CCwGG_T
Cuts at: 4187 4187
Size: 7168
SfcI C'TryA_G
Cuts at: 826 1045 2107 2467 2878 3739 4313 4516 5651
Size: 219 1062 360 411 861 574 203 1135
Cuts at: 5651 6332 6523 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1062 861 681 574 411 360
219 203 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7168
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7168
SmaI CCC'GGG
Cuts at: 1872 4495 1872
Size: 2623 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2632 3543 4423 5272 6173 6435
Size: 197 926 689 911 880 849 901 262
Cuts at: 6435 820
Size: 1553
Fragments arranged by size:
1553 926 911 901 880 849 689 262
197
SnaBI TAC'GTA
Cuts at: 493 4959 493
Size: 4466 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7168
SphI G_CATG'C
Cuts at: 1132 2561 4526 1132
Size: 1429 1965 3774
Fragments arranged by size:
3774 1965 1429
SspI AAT'ATT
Cuts at: 5 52 5092 5
Size: 47 5040 2081
Fragments arranged by size:
5040 2081 47
StuI AGG'CCT
Cuts at: 1517 4208 1517
Size: 2691 4477
StyI C'CwwG_G
Cuts at: 513 1065 2457 2788 3186 3369 4607 513
Size: 552 1392 331 398 183 1238 3074
Fragments arranged by size:
3074 1392 1238 552 398 331 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4231 5195 5212 5365 5550 5995 325
Size: 1255 2651 964 17 153 185 445 1498
Fragments arranged by size:
2651 1498 1255 964 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2393 5414 7126
Size: 274 80 33 51 1443 416 3021 1712
Cuts at: 7126 96
Size: 138
Fragments arranged by size:
3021 1712 1443 416 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2701 2733 3012 3044 3077 3109 7128 7160 2701
Size: 32 279 32 33 32 4019 32 2709
Fragments arranged by size:
4019 2709 279 33 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2363 2447 1316
Size: 1047 84 6037
Fragments arranged by size:
6037 1047 84
XbaI T'CTAG_A
Cuts at: 2498 4504 2498
Size: 2006 5162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3217 3217
Size: 7168
XhoI C'TCGA_G
Cuts at: 1943 3543 1943
Size: 1600 5568
XmaI C'CCGG_G
Cuts at: 1870 4493 1870
Size: 2623 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 5297 3914
Size: 1383 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3198 4976 276
Size: 53 83 186 936 1664 1778 2468
Fragments arranged by size:
2468 1778 1664 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I
Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacII
SfiI SfoI SrfI SwaI