Restriction map for
pFN21AE9447
(Circular) (Six-base) MAPSORT of: pFN21AE9447.seq Check: 2640 from: 1 to: 5911
pFN21AE9447 5911 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3721 278
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2913 3254 1937
Size: 976 341 4594
Fragments arranged by size:
4594 976 341
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 2419 4038 4411 1563
Size: 856 1619 373 3063
Fragments arranged by size:
3063 1619 856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2095 2702 3974 5358 5626 1632
Size: 463 607 1272 1384 268 1917
Fragments arranged by size:
1917 1384 1272 607 463 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5714
AflIII A'CryG_T
Cuts at: 1203 4810 5787 1203
Size: 3607 977 1327
Fragments arranged by size:
3607 1327 977
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 2503 1166
Size: 1337 4574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2198 1184
Size: 1014 4897
AlwNI CAG_nnn'CTG
Cuts at: 2974 3026 3654 5226 2974
Size: 52 628 1572 3659
Fragments arranged by size:
3659 1572 628 52
ApaLI G'TGCA_C
Cuts at: 3968 5124 3968
Size: 1156 4755
ApoI r'AATT_y
Cuts at: 1493 3220 3446 3598 5707 1493
Size: 1727 226 152 2109 1697
Fragments arranged by size:
2109 1727 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 3236 1870
Size: 73 68 1225 4545
Fragments arranged by size:
4545 1225 73 68
BamHI G'GATC_C
Cuts at: 3241 3608 3241
Size: 367 5544
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 2120 2641 2994 3232
Size: 325 217 627 290 43 521 353 238
Cuts at: 3232 4687 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 521 353 325 290 238
217 43
BanII G_rGCy'C
Cuts at: 1391 2099 2153 3230 1391
Size: 708 54 1077 4072
Fragments arranged by size:
4072 1077 708 54
BbeI G_GCGC'C
Cuts at: 2081 2081
Size: 5911
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2926 928
Size: 971 1027 3913
Fragments arranged by size:
3913 1027 971
BbvCI CC'TCA_GC
Cuts at: 2067 2258 2990 2067
Size: 191 732 4988
Fragments arranged by size:
4988 732 191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2356 2390 4102 4136 1903
Size: 34 419 34 1712 34 3678
Fragments arranged by size:
3678 1712 419 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 5013 3803
Size: 1210 4701
BclI T'GATC_A
Cuts at: 1548 2448 2733 2940 1548
Size: 900 285 207 4519
Fragments arranged by size:
4519 900 285 207
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2471 4522 136
Size: 107 122 71 631 605 799 2051 1525
Fragments arranged by size:
2051 1525 799 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2713 5906 2713
Size: 3193 2718
BlpI GC'TnA_GC
Cuts at: 3328 3328
Size: 5911
Bme1580I G_kGCm'C
Cuts at: 1790 2646 3972 5128 1790
Size: 856 1326 1156 2573
Fragments arranged by size:
2573 1326 1156 856
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5911
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2050 3642 4600
Size: 416 53 83 399 24 633 1592 958
Cuts at: 4600 442
Size: 1753
Fragments arranged by size:
1753 1592 958 633 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5911
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2537 2537
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2488 4571 1239
Size: 1249 2083 2579
Fragments arranged by size:
2579 2083 1249
Bpu10I CC'TnA_GC
Cuts at: 2067 2258 2990 2067
Size: 191 732 4988
Fragments arranged by size:
4988 732 191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4036 4901 5199 4036
Size: 865 298 4748
Fragments arranged by size:
4748 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4574 882
Size: 617 3075 2219
Fragments arranged by size:
3075 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3702 493
Size: 711 2498 2702
Fragments arranged by size:
2702 2498 711
BsaBI GATnn'nnATC
Cuts at: 5905 5905
Size: 5911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2078 2169 3718 4100
Size: 53 83 186 936 545 91 1549 382
Cuts at: 4100 4735 275
Size: 635 1451
Fragments arranged by size:
1549 1451 936 635 545 382 186 91
83 53
BsaWI w'CCGG_w
Cuts at: 4344 4742 5016 5163 5574 4344
Size: 398 274 147 411 4681
Fragments arranged by size:
4681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2588 2618 1178
Size: 30 165 30 1185 30 4471
Fragments arranged by size:
4471 1185 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 1186
Size: 1042 4869
BseYI C'CCAG_C
Cuts at: 2146 2164 2322 5114 5610 2146
Size: 18 158 2792 496 2447
Fragments arranged by size:
2792 2447 496 158 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2551 1163
Size: 573 815 4523
Fragments arranged by size:
4523 815 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3361 4122 5150 1540
Size: 275 186 1360 761 1028 2301
Fragments arranged by size:
2301 1360 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 2518 3972 4057 5128 2193
Size: 325 1454 85 1071 2976
Fragments arranged by size:
2976 1454 1071 325 85
BsmI GAATG_Cn'
Cuts at: 2700 3422 3515 2700
Size: 722 93 5096
Fragments arranged by size:
5096 722 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 2153 2193 2518 2646 3230 3972
Size: 399 309 54 40 325 128 584 742
Cuts at: 3972 4057 5128 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 584 399 325 309 128
85 54 40
BspEI T'CCGG_A
Cuts at: 5574 5574
Size: 5911
BspHI T'CATG_A
Cuts at: 2937 3800 5530 2937
Size: 863 1730 3318
Fragments arranged by size:
3318 1730 863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2190 2274 3266 844
Size: 1346 84 992 3489
Fragments arranged by size:
3489 1346 992 84
BsrBI CCG'CTC
Cuts at: 1394 1823 3798 1394
Size: 429 1975 3507
Fragments arranged by size:
3507 1975 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2388 2662 4406 4580 65
Size: 1183 1140 274 1744 174 1396
Fragments arranged by size:
1744 1396 1183 1140 274 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 2080 4555 4742 1700
Size: 110 264 6 2475 187 2869
Fragments arranged by size:
2869 2475 264 187 110 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5911
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5911
BssSI C'ACGA_G
Cuts at: 3971 4983 3971
Size: 1012 4899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5911
BstBI TT'CG_AA
Cuts at: 2692 2953 4715 2692
Size: 261 1762 3888
Fragments arranged by size:
3888 1762 261
BstEII G'GTnAC_C
Cuts at: 1184 2287 2852 1184
Size: 1103 565 4243
Fragments arranged by size:
4243 1103 565
BstXI CCAn_nnnn'nTGG
Cuts at: 2669 2669
Size: 5911
BstYI r'GATC_y
Cuts at: 1972 2713 2957 3241 3608 3993 4010 5462 5548
Size: 741 244 284 367 385 17 1452 86
Cuts at: 5548 5560 5906 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 741 385 367 346 284 244
86 17 12
BstZ17I GTA'TAC
Cuts at: 2914 2914
Size: 5911
Bsu36I CC'TnA_GG
Cuts at: 2312 2312
Size: 5911
BtgI C'CryG_G
Cuts at: 513 1065 2032 2058 2337 513
Size: 552 967 26 279 4087
Fragments arranged by size:
4087 967 552 279 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5172
Fragments arranged by size:
5172 683 56
BtsI GCAGTG_nn'
Cuts at: 3427 4221 4241 3427
Size: 794 20 5097
Fragments arranged by size:
5097 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5876
DraI TTT'AAA
Cuts at: 2745 3215 2745
Size: 470 5441
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4918 809
Size: 507 3602 1802
Fragments arranged by size:
3602 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 2671 2763
Size: 54 1425 50 275 18 199 642 92
Cuts at: 2763 3358 4247 5876 8
Size: 595 889 1629 43
Fragments arranged by size:
1629 1425 889 642 595 275 199 92
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3358 1537
Size: 275 1546 4090
Fragments arranged by size:
4090 1546 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 2285 2378 3112 3841 1353
Size: 761 171 93 734 729 3423
Fragments arranged by size:
3423 761 734 729 171 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4498 4872 5018 387
Size: 1064 138 2909 374 146 1280
Fragments arranged by size:
2909 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2488 2702 3974 4571 5358 5626
Size: 393 463 393 214 1272 597 787 268
Cuts at: 5626 1239
Size: 1524
Fragments arranged by size:
1524 1272 787 597 463 393 393 268
214
EcoNI CCTnn'n_nnAGG
Cuts at: 2311 2311
Size: 5911
EcoRI G'AATT_C
Cuts at: 3220 3598 3220
Size: 378 5533
FspI TGC'GCA
Cuts at: 4417 4417
Size: 5911
HaeII r_GCGC'y
Cuts at: 1678 2067 2081 2134 2141 5058 1678
Size: 389 14 53 7 2917 2531
Fragments arranged by size:
2917 2531 389 53 14 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2567 2599 4552 4584 4626
Size: 32 135 32 995 32 1953 32 42
Cuts at: 4626 4658 1373
Size: 32 2626
Fragments arranged by size:
2626 1953 995 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2248 3154 3255 3501 669
Size: 988 281 310 906 101 246 3079
Fragments arranged by size:
3079 988 906 310 281 246 101
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5911
HpaI GTT'AAC
Cuts at: 3501 3501
Size: 5911
KasI G'GCGC_C
Cuts at: 2077 2077
Size: 5911
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 5911
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2786 2891 3007 5025 5209 1305
Size: 1481 105 116 2018 184 2007
Fragments arranged by size:
2018 2007 1481 184 116 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2673 2765 10
Size: 54 1425 343 841 92 3156
Fragments arranged by size:
3156 1425 841 343 92 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2429 2503 2667 2693 3056 3869 4228
Size: 648 1263 74 164 26 363 813 359
Cuts at: 4228 4387 518
Size: 159 2042
Fragments arranged by size:
2042 1263 813 648 363 359 164 159
74 26
MspA1I CmG'CkG
Cuts at: 1606 2025 2060 2215 3026 3327 4004 5152 5397
Size: 419 35 155 811 301 677 1148 245
Cuts at: 5397 1606
Size: 2120
Fragments arranged by size:
2120 1148 811 677 419 301 245 155
35
NaeI GCC'GGC
Cuts at: 2076 2082 2076
Size: 6 5905
NarI GG'CG_CC
Cuts at: 2078 2078
Size: 5911
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5359
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5911
NgoMIV G'CCGG_C
Cuts at: 2074 2080 2074
Size: 6 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5911
NotI GC'GGCC_GC
Cuts at: 3358 3358
Size: 5911
NspI r_CATG'y
Cuts at: 1132 1229 3269 4814 1132
Size: 97 2040 1545 2229
Fragments arranged by size:
2229 2040 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2647 1348
Size: 1299 4612
PciI A'CATG_T
Cuts at: 4810 4810
Size: 5911
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3026 1108
Size: 1918 3993
PfoI T'CCnGG_A
Cuts at: 2544 2544
Size: 5911
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 5493 5525 3980
Size: 32 1481 32 4366
Fragments arranged by size:
4366 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5911
PsiI TTA'TAA
Cuts at: 3481 3481
Size: 5911
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2897 2929 3183 3215 2897
Size: 32 254 32 5593
Fragments arranged by size:
5593 254 32 32
PstI C_TGCA'G
Cuts at: 830 3263 830
Size: 2433 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
PvuII CAG'CTG
Cuts at: 3026 3026
Size: 5911
SacII CC_GC'GG
Cuts at: 2061 2061
Size: 5911
SalI G'TCGA_C
Cuts at: 1936 3253 1936
Size: 1317 4594
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SexAI A'CCwGG_T
Cuts at: 2856 2856
Size: 5911
SfcI C'TryA_G
Cuts at: 826 1045 3259 4394 5075 5266 826
Size: 219 2214 1135 681 191 1471
Fragments arranged by size:
2214 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2471 2471
Size: 5911
SfoI GGC'GCC
Cuts at: 2079 2079
Size: 5911
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 2080 1700
Size: 380 5531
SmaI CCC'GGG
Cuts at: 1872 3238 1872
Size: 1366 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4015 4916 5178 820
Size: 197 926 2072 901 262 1553
Fragments arranged by size:
2072 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 3269 1132
Size: 2137 3774
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 3004 1517
Size: 1487 4424
StyI C'CwwG_G
Cuts at: 513 1065 2586 3350 513
Size: 552 1521 764 3074
Fragments arranged by size:
3074 1521 764 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2314 2639 3938 3955 4108 4293 4738
Size: 1255 734 325 1299 17 153 185 445
Cuts at: 4738 325
Size: 1498
Fragments arranged by size:
1498 1299 1255 734 445 325 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 4157 5869
Size: 274 80 33 51 1443 30 2150 1712
Cuts at: 5869 96
Size: 138
Fragments arranged by size:
2150 1712 1443 274 138 80 51 33
30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5871 5903 5871
Size: 32 5879
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5911
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2161 2161
Size: 5911
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5911
XmaI C'CCGG_G
Cuts at: 1870 3236 1870
Size: 1366 4545
XmnI GAAnn'nnTTC
Cuts at: 2715 4040 2715
Size: 1325 4586
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3719 276
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI DraIII EcoICRI EcoO109I EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI
RsrII SacI SanDI SapI SrfI SwaI