Restriction map for
pFN21AE9446
(Circular) (Six-base) MAPSORT of: pFN21AE9446.seq Check: 9929 from: 1 to: 5836
pFN21AE9446 5836 bp
With 182 enzymes: SgfI *
March 28, 2012 12:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2457 3179 1937
Size: 520 722 4594
Fragments arranged by size:
4594 722 520
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2986 3899 5283 5551 1632
Size: 492 862 913 1384 268 1917
Fragments arranged by size:
1917 1384 913 862 492 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 2059 2956 3579 5151 2059
Size: 897 623 1572 2744
Fragments arranged by size:
2744 1572 897 623
ApaI G_GGCC'C
Cuts at: 2602 2602
Size: 5836
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 3145 3371 3523 5632 1493
Size: 1652 226 152 2109 1697
Fragments arranged by size:
2109 1697 1652 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2322 4390 160
Size: 872 1290 2068 1606
Fragments arranged by size:
2068 1606 1290 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 3161 1870
Size: 73 1218 4545
Fragments arranged by size:
4545 1218 73
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2350 3157 4612 618
Size: 325 217 627 563 807 1455 1842
Fragments arranged by size:
1842 1455 807 627 563 325 217
BanII G_rGCy'C
Cuts at: 1391 2602 3127 3155 1391
Size: 1211 525 28 4072
Fragments arranged by size:
4072 1211 525 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2992 928
Size: 971 1093 3772
Fragments arranged by size:
3772 1093 971
BbvCI CC'TCA_GC
Cuts at: 2429 2429
Size: 5836
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4027 4061 1903
Size: 34 50 34 2006 34 3678
Fragments arranged by size:
3678 2006 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 2550 2943 1548
Size: 1002 393 4441
Fragments arranged by size:
4441 1002 393
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4447 136
Size: 107 122 71 631 605 2775 1525
Fragments arranged by size:
2775 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5831 5831
Size: 5836
BlpI GC'TnA_GC
Cuts at: 3253 3253
Size: 5836
Bme1580I G_kGCm'C
Cuts at: 1790 2602 3897 5053 1790
Size: 812 1295 1156 2573
Fragments arranged by size:
2573 1295 1156 812
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5836
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2066 3567 4525
Size: 416 53 83 399 24 649 1501 958
Cuts at: 4525 442
Size: 1753
Fragments arranged by size:
1753 1501 958 649 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2358 2536 3035 4496 1239
Size: 1119 178 499 1461 2579
Fragments arranged by size:
2579 1461 1119 499 178
Bpu10I CC'TnA_GC
Cuts at: 2429 2429
Size: 5836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3961 4826 5124 3961
Size: 865 298 4673
Fragments arranged by size:
4673 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4499 882
Size: 617 3000 2219
Fragments arranged by size:
3000 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3627 493
Size: 711 2423 2702
Fragments arranged by size:
2702 2423 711
BsaBI GATnn'nnATC
Cuts at: 5830 5830
Size: 5836
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3643 4025 4660 275
Size: 53 83 186 936 2110 382 635 1451
Fragments arranged by size:
2110 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4269 4667 4941 5088 5499 4269
Size: 398 274 147 411 4606
Fragments arranged by size:
4606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5611
Fragments arranged by size:
5611 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2245 1186
Size: 1059 4777
BseYI C'CCAG_C
Cuts at: 2595 5039 5535 2595
Size: 2444 496 2896
Fragments arranged by size:
2896 2444 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2609 2794 1163
Size: 573 873 185 4205
Fragments arranged by size:
4205 873 573 185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4047 5075 1540
Size: 275 186 1285 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3897 3982 5053 3897
Size: 85 1071 4680
Fragments arranged by size:
4680 1071 85
BsmI GAATG_Cn'
Cuts at: 3347 3440 3347
Size: 93 5743
BsmBI CGTCTCn'nnnn_
Cuts at: 3044 3044
Size: 5836
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2602 3127 3155 3897 3982 5053 1391
Size: 399 812 525 28 742 85 1071 2174
Fragments arranged by size:
2174 1071 812 742 525 399 85 28
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 3725 5455 3725
Size: 1730 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2377 2964 3191 844
Size: 1533 587 227 3489
Fragments arranged by size:
3489 1533 587 227
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4331 4505 65
Size: 1183 3083 174 1396
Fragments arranged by size:
3083 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4480 4667 1700
Size: 110 2670 187 2869
Fragments arranged by size:
2869 2670 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 3896 4908 3896
Size: 1012 4824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2392 794
Size: 1598 4238
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstYI r'GATC_y
Cuts at: 1972 3166 3533 3918 3935 5387 5473 5485 5831
Size: 1194 367 385 17 1452 86 12 346
Cuts at: 5831 1972
Size: 1977
Fragments arranged by size:
1977 1452 1194 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 2597 3352 4146 4166 2597
Size: 755 794 20 4267
Fragments arranged by size:
4267 794 755 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2274 3104 3283
Size: 54 1425 50 275 18 444 830 179
Cuts at: 3283 4172 5801 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 830 444 275 179 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2558 3132 3766 1353
Size: 1205 574 634 3423
Fragments arranged by size:
3423 1205 634 574
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4423 4797 4943 387
Size: 1064 138 2834 374 146 1280
Fragments arranged by size:
2834 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2358 2536 2986 3035 3899 4496
Size: 393 492 234 178 450 49 864 597
Cuts at: 4496 5283 5551 1239
Size: 787 268 1524
Fragments arranged by size:
1524 864 787 597 492 450 393 268
234 178 49
EcoO109I rG'GnC_Cy
Cuts at: 2176 2497 2599 2176
Size: 321 102 5413
Fragments arranged by size:
5413 321 102
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 4983 1678
Size: 3305 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2781 2813 4477 4509 4551
Size: 32 135 32 1209 32 1664 32 42
Cuts at: 4551 4583 1373
Size: 32 2626
Fragments arranged by size:
2626 1664 1209 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2305 3180 3426 669
Size: 988 281 367 875 246 3079
Fragments arranged by size:
3079 988 875 367 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 2159 3435 2159
Size: 1276 4560
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2179 2601 2775 2843 4950 5134 1305
Size: 874 422 174 68 2107 184 2007
Fragments arranged by size:
2107 2007 874 422 184 174 68
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2276 3106 10
Size: 54 1425 343 444 830 2740
Fragments arranged by size:
2740 1425 830 444 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3794 4153 4312 518
Size: 648 2628 359 159 2042
Fragments arranged by size:
2628 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2272 3252 3929 5077 5322 1606
Size: 666 980 677 1148 245 2120
Fragments arranged by size:
2120 1148 980 677 666 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5284
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3194 4739 1132
Size: 97 1965 1545 2229
Fragments arranged by size:
2229 1965 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5836
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5836
PfoI T'CCnGG_A
Cuts at: 3051 3051
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 5418 5450 3905
Size: 32 1481 32 4291
Fragments arranged by size:
4291 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2176 2497 2176
Size: 321 5515
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PspOMI G'GGCC_C
Cuts at: 2598 2598
Size: 5836
PstI C_TGCA'G
Cuts at: 830 3029 3188 830
Size: 2199 159 3478
Fragments arranged by size:
3478 2199 159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2272 2272
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SapI GCTCTTCn'nnn_
Cuts at: 3132 3132
Size: 5836
SbfI CC_TGCA'GG
Cuts at: 3029 3188 3029
Size: 159 5677
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SexAI A'CCwGG_T
Cuts at: 2358 2358
Size: 5836
SfcI C'TryA_G
Cuts at: 826 1045 3025 3184 4319 5000 5191 826
Size: 219 1980 159 1135 681 191 1471
Fragments arranged by size:
1980 1471 1135 681 219 191 159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 3163 1872
Size: 1291 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3940 4841 5103 820
Size: 197 926 1997 901 262 1553
Fragments arranged by size:
1997 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5836
StyI C'CwwG_G
Cuts at: 513 1065 3131 3275 513
Size: 552 2066 144 3074
Fragments arranged by size:
3074 2066 552 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2933 3863 3880 4033 4218 4663 325
Size: 1255 1353 930 17 153 185 445 1498
Fragments arranged by size:
1498 1353 1255 930 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2658 4082 5794
Size: 274 80 33 51 1443 681 1424 1712
Cuts at: 5794 96
Size: 138
Fragments arranged by size:
1712 1443 1424 681 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5796 5828 5796
Size: 32 5804
Tth111I GACn'n_nGTC
Cuts at: 1316 2572 1316
Size: 1256 4580
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 3161 1870
Size: 1291 4545
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI XcmI