Restriction map for
pFN21AE9444
(Circular) (Six-base) MAPSORT of: pFN21AE9444.seq Check: 5986 from: 1 to: 9112
pFN21AE9444 9112 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2154 6922 278
Size: 53 83 186 936 618 4768 2468
Fragments arranged by size:
4768 2468 936 618 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2430 5007 6006 6455 1937
Size: 493 2577 999 449 4594
Fragments arranged by size:
4594 2577 999 493 449
Acc65I G'GTAC_C
Cuts at: 6433 6433
Size: 9112
AclI AA'CG_TT
Cuts at: 1563 7239 7612 1563
Size: 5676 373 3063
Fragments arranged by size:
5676 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3860 3926 5804 7175 8559 8827 1632
Size: 2228 66 1878 1371 1384 268 1917
Fragments arranged by size:
2228 1917 1878 1384 1371 268 66
AfeI AGC'GCT
Cuts at: 3890 5834 3890
Size: 1944 7168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8915
AflIII A'CryG_T
Cuts at: 1203 4591 8011 8988 1203
Size: 3388 3420 977 1327
Fragments arranged by size:
3420 3388 1327 977
AgeI A'CCGG_T
Cuts at: 7943 7943
Size: 9112
AhdI GACnn_n'nnGTC
Cuts at: 7841 7841
Size: 9112
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4629 5741 1184
Size: 3445 1112 4555
Fragments arranged by size:
4555 3445 1112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6324 6356 6324
Size: 32 9080
AlwNI CAG_nnn'CTG
Cuts at: 2312 3651 6855 8427 2312
Size: 1339 3204 1572 2997
Fragments arranged by size:
3204 2997 1572 1339
ApaLI G'TGCA_C
Cuts at: 7169 8325 7169
Size: 1156 7956
ApoI r'AATT_y
Cuts at: 1493 2196 2958 3618 4145 4441 4570 4656 6421
Size: 703 762 660 527 296 129 86 1765
Cuts at: 6421 6647 6799 8908 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1765 1697 762 703 660 527 296
226 152 129 86
AseI AT'TA_AT
Cuts at: 160 1032 5295 7666 160
Size: 872 4263 2371 1606
Fragments arranged by size:
4263 2371 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9112
AvaI C'yCGr_G
Cuts at: 1870 1943 3513 6368 6437 1870
Size: 73 1570 2855 69 4545
Fragments arranged by size:
4545 2855 1570 73 69
AvrII C'CTAG_G
Cuts at: 6265 6265
Size: 9112
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5631 5664 5631
Size: 33 9079
BamHI G'GATC_C
Cuts at: 3625 6051 6442 6809 3625
Size: 2426 391 367 5928
Fragments arranged by size:
5928 2426 391 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6433 7888 618
Size: 325 217 627 4646 1455 1842
Fragments arranged by size:
4646 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3356 4719 6431 1391
Size: 1965 1363 1712 4072
Fragments arranged by size:
4072 1965 1712 1363
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2593 4067 4143 5559 928
Size: 971 694 1474 76 1416 4481
Fragments arranged by size:
4481 1474 1416 971 694 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5409 5443 7303 7337 1903
Size: 34 3472 34 1860 34 3678
Fragments arranged by size:
3678 3472 1860 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2902 5632 5647 7004 8214 2902
Size: 2730 15 1357 1210 3800
Fragments arranged by size:
3800 2730 1357 1210 15
BclI T'GATC_A
Cuts at: 1548 3985 4641 1548
Size: 2437 656 6019
Fragments arranged by size:
6019 2437 656
BfrBI ATG'CAT
Cuts at: 3962 3962
Size: 9112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7723 136
Size: 107 122 71 631 605 6051 1525
Fragments arranged by size:
6051 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3640 6109 9107 3640
Size: 2469 2998 3645
Fragments arranged by size:
3645 2998 2469
BlpI GC'TnA_GC
Cuts at: 6529 6529
Size: 9112
Bme1580I G_kGCm'C
Cuts at: 1790 2074 7173 8329 1790
Size: 284 5099 1156 2573
Fragments arranged by size:
5099 2573 1156 284
BmgBI CAC'GTC
Cuts at: 1318 3154 6283 1318
Size: 1836 3129 4147
Fragments arranged by size:
4147 3129 1836
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2284 6843 7801
Size: 416 53 83 399 24 867 4559 958
Cuts at: 7801 442
Size: 1753
Fragments arranged by size:
4559 1753 958 867 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6152 6152
Size: 9112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3402 4703 4904 5601 5821 6098 6256 7772
Size: 2163 1301 201 697 220 277 158 1516
Cuts at: 7772 1239
Size: 2579
Fragments arranged by size:
2579 2163 1516 1301 697 277 220 201
158
Bpu10I CC'TnA_GC
Cuts at: 5714 5714
Size: 9112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7237 8102 8400 7237
Size: 865 298 7949
Fragments arranged by size:
7949 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3963 5753 7775 882
Size: 617 2464 1790 2022 2219
Fragments arranged by size:
2464 2219 2022 1790 617
BsaAI yAC'GTr
Cuts at: 493 1204 4647 4940 6903 493
Size: 711 3443 293 1963 2702
Fragments arranged by size:
3443 2702 1963 711 293
BsaBI GATnn'nnATC
Cuts at: 4688 4793 9106 4688
Size: 105 4313 4694
Fragments arranged by size:
4694 4313 105
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 6919 7301 7936
Size: 53 83 186 936 618 4768 382 635
Cuts at: 7936 275
Size: 1451
Fragments arranged by size:
4768 1451 936 635 618 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3396 7545 7943 8217 8364 8775 3396
Size: 4149 398 274 147 411 3733
Fragments arranged by size:
4149 3733 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3391 3400 3421 3430 6254
Size: 30 165 30 1988 9 21 9 2824
Cuts at: 6254 6284 1178
Size: 30 4006
Fragments arranged by size:
4006 2824 1988 165 30 30 30 21
9 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5957 1186
Size: 4771 4341
BseYI C'CCAG_C
Cuts at: 3478 3758 8315 8811 3478
Size: 280 4557 496 3779
Fragments arranged by size:
4557 3779 496 280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2316 2381 4913 4962 1163
Size: 573 580 65 2532 49 5313
Fragments arranged by size:
5313 2532 580 573 65 49
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6562 7323 8351 1540
Size: 275 186 4561 761 1028 2301
Fragments arranged by size:
4561 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4121 6102 7173 7258 8329 4121
Size: 1981 1071 85 1071 4904
Fragments arranged by size:
4904 1981 1071 1071 85
BsmI GAATG_Cn'
Cuts at: 3760 3964 4285 6623 6716 3760
Size: 204 321 2338 93 6156
Fragments arranged by size:
6156 2338 321 204 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 3356 4121 4719 6102 6431 7173
Size: 399 284 1282 765 598 1383 329 742
Cuts at: 7173 7258 8329 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1383 1282 1071 765 742 598 399
329 284 85
BspEI T'CCGG_A
Cuts at: 8775 8775
Size: 9112
BspHI T'CATG_A
Cuts at: 2031 2157 5968 7001 8731 2031
Size: 126 3811 1033 1730 2412
Fragments arranged by size:
3811 2412 1730 1033 126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2297 6467 844
Size: 1453 4170 3489
Fragments arranged by size:
4170 3489 1453
BsrBI CCG'CTC
Cuts at: 1394 1823 6999 1394
Size: 429 5176 3507
Fragments arranged by size:
5176 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2577 3093 3714 3921 5425 5694 7607
Size: 1183 1329 516 621 207 1504 269 1913
Cuts at: 7607 7781 65
Size: 174 1396
Fragments arranged by size:
1913 1504 1396 1329 1183 621 516 269
207 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6214 6388 7756 7943 1700
Size: 110 4404 174 1368 187 2869
Fragments arranged by size:
4404 2869 1368 187 174 110
BsrGI T'GTAC_A
Cuts at: 96 3814 96
Size: 3718 5394
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9112
BssSI C'ACGA_G
Cuts at: 5939 7172 8184 5939
Size: 1233 1012 6867
Fragments arranged by size:
6867 1233 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9112
BstBI TT'CG_AA
Cuts at: 5551 7916 5551
Size: 2365 6747
BstEII G'GTnAC_C
Cuts at: 1184 5672 1184
Size: 4488 4624
BstXI CCAn_nnnn'nTGG
Cuts at: 2468 5914 2468
Size: 3446 5666
BstYI r'GATC_y
Cuts at: 1972 3625 3640 6051 6109 6442 6809 7194 7211
Size: 1653 15 2411 58 333 367 385 17
Cuts at: 7211 8663 8749 8761 9107 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2411 1977 1653 1452 385 367 346 333
86 58 17 15 12
BstZ17I GTA'TAC
Cuts at: 2431 6007 2431
Size: 3576 5536
BtgI C'CryG_G
Cuts at: 513 1065 3786 4165 4272 4711 5677 5740 513
Size: 552 2721 379 107 439 966 63 3885
Fragments arranged by size:
3885 2721 966 552 439 379 107 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 1998 487
Size: 683 56 772 7601
Fragments arranged by size:
7601 772 683 56
BtsI GCAGTG_nn'
Cuts at: 2304 5893 6331 6628 7422 7442 2304
Size: 3589 438 297 794 20 3974
Fragments arranged by size:
3974 3589 794 438 297 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2070 2105 3936 3971 529
Size: 35 1506 35 1831 35 5670
Fragments arranged by size:
5670 1831 1506 35 35 35
DraI TTT'AAA
Cuts at: 6416 6416
Size: 9112
DraIII CAC_nnn'GTG
Cuts at: 3157 3157
Size: 9112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8119 809
Size: 507 6803 1802
Fragments arranged by size:
6803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2009 3999 4210
Size: 54 1425 50 275 18 179 1990 211
Cuts at: 4210 6168 6216 6559 7448 9077 8
Size: 1958 48 343 889 1629 43
Fragments arranged by size:
1990 1958 1629 1425 889 343 275 211
179 54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6559 1537
Size: 275 4747 4090
Fragments arranged by size:
4747 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3483 4787 5253 5330 7042 1353
Size: 2130 1304 466 77 1712 3423
Fragments arranged by size:
3423 2130 1712 1304 466 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3225 6064 7699 8073 8219 387
Size: 1064 138 1636 2839 1635 374 146 1280
Fragments arranged by size:
2839 1636 1635 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3402 3860 3926 4703 4904 5601 5804
Size: 393 1770 458 66 777 201 697 203
Cuts at: 5804 5821 6098 6256 7175 7772 8559 8827 1239
Size: 17 277 158 919 597 787 268 1524
Fragments arranged by size:
1770 1524 919 787 777 697 597 458
393 277 268 203 201 158 66 17
EcoNI CCTnn'n_nnAGG
Cuts at: 5172 5172
Size: 9112
EcoO109I rG'GnC_Cy
Cuts at: 2309 3296 4259 6360 2309
Size: 987 963 2101 5061
Fragments arranged by size:
5061 2101 987 963
EcoRI G'AATT_C
Cuts at: 2958 6421 6799 2958
Size: 3463 378 5271
Fragments arranged by size:
5271 3463 378
EcoRV GAT'ATC
Cuts at: 3283 3283
Size: 9112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2420 2590 2949 2420
Size: 170 359 8583
Fragments arranged by size:
8583 359 170
FspI TGC'GCA
Cuts at: 7618 7618
Size: 9112
HaeII r_GCGC'y
Cuts at: 1678 3892 5836 8259 1678
Size: 2214 1944 2423 2531
Fragments arranged by size:
2531 2423 2214 1944
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2251 2283 2450 2482 2546
Size: 32 135 32 679 32 167 32 64
Cuts at: 2546 2578 2715 2747 2912 2944 3179 3211 3730
Size: 32 137 32 165 32 235 32 519
Cuts at: 3730 3762 3891 3923 5202 5234 5912 5944 6101
Size: 32 129 32 1279 32 678 32 157
Cuts at: 6101 6133 7753 7785 7827 7859 1373
Size: 32 1620 32 42 32 2626
Fragments arranged by size:
2626 1620 1279 679 678 519 235 167
165 157 137 135 129 64 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3931 4503 6456 6702 669
Size: 988 281 1993 572 1953 246 3079
Fragments arranged by size:
3079 1993 1953 988 572 281 246
HindIII A'AGCT_T
Cuts at: 748 3872 3951 4305 748
Size: 3124 79 354 5555
Fragments arranged by size:
5555 3124 354 79
HpaI GTT'AAC
Cuts at: 3931 6702 3931
Size: 2771 6341
KpnI G_GTAC'C
Cuts at: 6437 6437
Size: 9112
MfeI C'AATT_G
Cuts at: 2870 6711 2870
Size: 3841 5271
MluI A'CGCG_T
Cuts at: 8988 8988
Size: 9112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2481 4633 5071 5823 8226 8410 1305
Size: 1176 2152 438 752 2403 184 2007
Fragments arranged by size:
2403 2152 2007 1176 752 438 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2011 10
Size: 54 1425 343 179 7111
Fragments arranged by size:
7111 1425 343 179 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3753 4611 4874 4910 7070 7429 7588
Size: 648 2587 858 263 36 2160 359 159
Cuts at: 7588 518
Size: 2042
Fragments arranged by size:
2587 2160 2042 858 648 359 263 159
36
MspA1I CmG'CkG
Cuts at: 1606 3101 3422 3707 3796 3991 4183 4713 5000
Size: 1495 321 285 89 195 192 530 287
Cuts at: 5000 5703 6292 6528 7205 8353 8598 1606
Size: 703 589 236 677 1148 245 2120
Fragments arranged by size:
2120 1495 1148 703 677 589 530 321
287 285 245 236 195 192 89
NcoI C'CATG_G
Cuts at: 513 1065 3786 4165 5677 513
Size: 552 2721 379 1512 3948
Fragments arranged by size:
3948 2721 1512 552 379
NdeI CA'TA_TG
Cuts at: 387 2917 387
Size: 2530 6582
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9112
NotI GC'GGCC_GC
Cuts at: 6559 6559
Size: 9112
NsiI A_TGCA'T
Cuts at: 3964 3964
Size: 9112
NspI r_CATG'y
Cuts at: 1132 1229 4551 4595 6151 6259 6470 8015 1132
Size: 97 3322 44 1556 108 211 1545 2229
Fragments arranged by size:
3322 2229 1556 1545 211 108 97 44
PasI CC'CwG_GG
Cuts at: 1348 3117 1348
Size: 1769 7343
PciI A'CATG_T
Cuts at: 4591 8011 4591
Size: 3420 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3916 5669 1108
Size: 2808 1753 4551
Fragments arranged by size:
4551 2808 1753
PfoI T'CCnGG_A
Cuts at: 2140 2335 2410 4881 5491 5913 2140
Size: 195 75 2471 610 422 5339
Fragments arranged by size:
5339 2471 610 422 195 75
PmeI GTTT'AAAC
Cuts at: 6416 6416
Size: 9112
PmlI CAC'GTG
Cuts at: 4647 4647
Size: 9112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7181 7213 8694 8726 7181
Size: 32 1481 32 7567
Fragments arranged by size:
7567 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2309 3296 4259 6360 2309
Size: 987 963 2101 5061
Fragments arranged by size:
5061 2101 987 963
PshAI GACnn'nnGTC
Cuts at: 1613 2331 1613
Size: 718 8394
PsiI TTA'TAA
Cuts at: 6682 6682
Size: 9112
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2893 2925 5437 5469 2893
Size: 32 2512 32 6536
Fragments arranged by size:
6536 2512 32 32
PstI C_TGCA'G
Cuts at: 830 2496 3574 5005 6464 830
Size: 1666 1078 1431 1459 3478
Fragments arranged by size:
3478 1666 1459 1431 1078
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9112
PvuII CAG'CTG
Cuts at: 3101 3422 3707 3796 3991 4183 5000 6292 3101
Size: 321 285 89 195 192 817 1292 5921
Fragments arranged by size:
5921 1292 817 321 285 195 192 89
SacII CC_GC'GG
Cuts at: 4714 4714
Size: 9112
SalI G'TCGA_C
Cuts at: 1936 6454 1936
Size: 4518 4594
SanDI GG'GwC_CC
Cuts at: 6360 6360
Size: 9112
SapI GCTCTTCn'nnn_
Cuts at: 4787 5253 4787
Size: 466 8646
SbfI CC_TGCA'GG
Cuts at: 3574 6464 3574
Size: 2890 6222
ScaI AGT'ACT
Cuts at: 3403 4581 7360 9072 3403
Size: 1178 2779 1712 3443
Fragments arranged by size:
3443 2779 1712 1178
SfcI C'TryA_G
Cuts at: 826 1045 2124 2492 2533 3570 3579 5001 5011
Size: 219 1079 368 41 1037 9 1422 10
Cuts at: 5011 5345 5381 5431 6460 7595 8276 8467 826
Size: 334 36 50 1029 1135 681 191 1471
Fragments arranged by size:
1471 1422 1135 1079 1037 1029 681 368
334 219 191 50 41 36 10 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9112
SmaI CCC'GGG
Cuts at: 1872 6439 1872
Size: 4567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 7216 8117 8379 820
Size: 197 926 5273 901 262 1553
Fragments arranged by size:
5273 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6903 493
Size: 6410 2702
SpeI A'CTAG_T
Cuts at: 152 3404 6404 152
Size: 3252 3000 2860
Fragments arranged by size:
3252 3000 2860
SphI G_CATG'C
Cuts at: 1132 6151 6259 6470 1132
Size: 5019 108 211 3774
Fragments arranged by size:
5019 3774 211 108
SspI AAT'ATT
Cuts at: 5 52 3164 4093 7036 5
Size: 47 3112 929 2943 2081
Fragments arranged by size:
3112 2943 2081 929 47
StuI AGG'CCT
Cuts at: 1517 3804 1517
Size: 2287 6825
StyI C'CwwG_G
Cuts at: 513 1065 2565 3631 3662 3786 4165 5119 5677
Size: 552 1500 1066 31 124 379 954 558
Cuts at: 5677 5881 6265 6551 513
Size: 204 384 286 3074
Fragments arranged by size:
3074 1500 1066 954 558 552 384 379
286 204 124 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6212 6218 7139 7156 7309 7494 7939
Size: 1255 4632 6 921 17 153 185 445
Cuts at: 7939 325
Size: 1498
Fragments arranged by size:
4632 1498 1255 921 445 185 153 17
6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3090 3401 3814
Size: 274 80 33 51 1443 1113 311 413
Cuts at: 3814 4579 5444 7358 9070 96
Size: 765 865 1914 1712 138
Fragments arranged by size:
1914 1712 1443 1113 865 765 413 311
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2625 2657 2704 2736 3439 3471 9072 9104 2625
Size: 32 47 32 703 32 5601 32 2633
Fragments arranged by size:
5601 2633 703 47 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4070 1316
Size: 2754 6358
XbaI T'CTAG_A
Cuts at: 6448 6448
Size: 9112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3793 4291 3793
Size: 498 8614
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9112
XmaI C'CCGG_G
Cuts at: 1870 6437 1870
Size: 4567 4545
XmnI GAAnn'nnTTC
Cuts at: 5964 7241 5964
Size: 1277 7835
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2152 6920 276
Size: 53 83 186 936 618 4768 2468
Fragments arranged by size:
4768 2468 936 618 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BbeI BbvCI BsiWI BsmBI Bsu36I
EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PspOMI PspXI RsrII SacI SexAI SfiI SfoI
SrfI SwaI