Restriction map for
pFN21AE9442
(Circular) (Six-base) MAPSORT of: pFN21AE9442.seq Check: 899 from: 1 to: 7606
pFN21AE9442 7606 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2277 4832 2277
Size: 2555 5051
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5416 278
Size: 53 83 186 936 3880 2468
Fragments arranged by size:
3880 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4949 1937
Size: 3012 4594
Acc65I G'GTAC_C
Cuts at: 4927 4927
Size: 7606
AclI AA'CG_TT
Cuts at: 1563 5733 6106 1563
Size: 4170 373 3063
Fragments arranged by size:
4170 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2231 3169 3368 3516 4674 5669 7053 7321
Size: 599 938 199 148 1158 995 1384 268
Cuts at: 7321 1632
Size: 1917
Fragments arranged by size:
1917 1384 1158 995 938 599 268 199
148
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7409
AflIII A'CryG_T
Cuts at: 1203 2616 4900 6505 7482 1203
Size: 1413 2284 1605 977 1327
Fragments arranged by size:
2284 1605 1413 1327 977
AgeI A'CCGG_T
Cuts at: 6437 6437
Size: 7606
AhdI GACnn_n'nnGTC
Cuts at: 3882 6335 3882
Size: 2453 5153
AleI CACnn'nnGTG
Cuts at: 1166 3627 1166
Size: 2461 5145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3044 4018 1184
Size: 1860 974 4772
Fragments arranged by size:
4772 1860 974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2777 2778 2809 2810 2777
Size: 1 31 1 7573
Fragments arranged by size:
7573 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2431 2677 2788 2914 3583 4612 5349 6921 2431
Size: 246 111 126 669 1029 737 1572 3116
Fragments arranged by size:
3116 1572 1029 737 669 246 126 111
ApaI G_GGCC'C
Cuts at: 2100 2548 4554 4732 2100
Size: 448 2006 178 4974
Fragments arranged by size:
4974 2006 448 178
ApaLI G'TGCA_C
Cuts at: 5663 6819 5663
Size: 1156 6450
ApoI r'AATT_y
Cuts at: 1493 4915 5141 5293 7402 1493
Size: 3422 226 152 2109 1697
Fragments arranged by size:
3422 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6160 160
Size: 872 5128 1606
Fragments arranged by size:
5128 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7606
AvaI C'yCGr_G
Cuts at: 1870 1943 2092 2168 4418 4931 1870
Size: 73 149 76 2250 513 4545
Fragments arranged by size:
4545 2250 513 149 76 73
AvrII C'CTAG_G
Cuts at: 4075 4075
Size: 7606
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4466 4499 4466
Size: 33 7573
BamHI G'GATC_C
Cuts at: 3411 4936 5303 3411
Size: 1525 367 5714
Fragments arranged by size:
5714 1525 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2945 2981 3421 3821
Size: 325 217 627 288 870 36 440 400
Cuts at: 3821 4250 4927 6382 618
Size: 429 677 1455 1842
Fragments arranged by size:
1842 1455 870 677 627 440 429 400
325 288 217 36
BanII G_rGCy'C
Cuts at: 1391 2025 2100 2169 2548 3200 3594 3907 4356
Size: 634 75 69 379 652 394 313 449
Cuts at: 4356 4554 4592 4732 4925 1391
Size: 198 38 140 193 4072
Fragments arranged by size:
4072 652 634 449 394 379 313 198
193 140 75 69 38
BbeI G_GCGC'C
Cuts at: 2079 3825 2079
Size: 1746 5860
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3504 3516 928
Size: 971 1605 12 5018
Fragments arranged by size:
5018 1605 971 12
BbvCI CC'TCA_GC
Cuts at: 2222 4379 4817 2222
Size: 2157 438 5011
Fragments arranged by size:
5011 2157 438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5797 5831 1903
Size: 34 50 34 3776 34 3678
Fragments arranged by size:
3776 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5498 6708 5498
Size: 1210 6396
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7606
BfrBI ATG'CAT
Cuts at: 3343 3343
Size: 7606
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2111 2570 3710
Size: 107 122 71 631 605 439 459 1140
Cuts at: 3710 4672 6217 136
Size: 962 1545 1525
Fragments arranged by size:
1545 1525 1140 962 631 605 459 439
122 107 71
BglII A'GATC_T
Cuts at: 2971 7601 2971
Size: 4630 2976
BlpI GC'TnA_GC
Cuts at: 3194 5023 3194
Size: 1829 5777
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2548 4554 4732 5667 6823 1790
Size: 310 448 2006 178 935 1156 2573
Fragments arranged by size:
2573 2006 1156 935 448 310 178
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7606
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2798 3621 5337
Size: 416 53 83 399 24 1381 823 1716
Cuts at: 5337 6295 442
Size: 958 1753
Fragments arranged by size:
1753 1716 1381 958 823 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7606
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3274 4606 3274
Size: 1332 6274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2732 3014 3437 3578 3620 3633 4601 4697
Size: 1493 282 423 141 42 13 968 96
Cuts at: 4697 4760 6266 1239
Size: 63 1506 2579
Fragments arranged by size:
2579 1506 1493 968 423 282 141 96
63 42 13
Bpu10I CC'TnA_GC
Cuts at: 2222 4379 4817 2222
Size: 2157 438 5011
Fragments arranged by size:
5011 2157 438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5731 6596 6894 5731
Size: 865 298 6443
Fragments arranged by size:
6443 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2345 4673 6269 882
Size: 617 846 2328 1596 2219
Fragments arranged by size:
2328 2219 1596 846 617
BsaAI yAC'GTr
Cuts at: 493 1204 4327 4903 5397 493
Size: 711 3123 576 494 2702
Fragments arranged by size:
3123 2702 711 576 494
BsaBI GATnn'nnATC
Cuts at: 4632 7600 4632
Size: 2968 4638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 3822 5413 5795
Size: 53 83 186 936 543 1746 1591 382
Cuts at: 5795 6430 275
Size: 635 1451
Fragments arranged by size:
1746 1591 1451 936 635 543 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2763 3234 6039 6437 6711 6858 7269 2763
Size: 471 2805 398 274 147 411 3100
Fragments arranged by size:
3100 2805 471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2438 2468 4127 4157 1178
Size: 30 165 30 1035 30 1659 30 4627
Fragments arranged by size:
4627 1659 1035 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2485 3113 3533 4346 1186
Size: 1111 188 628 420 813 4446
Fragments arranged by size:
4446 1111 813 628 420 188
BseYI C'CCAG_C
Cuts at: 2009 2082 2380 3107 3687 3909 4396 4658 6809
Size: 73 298 727 580 222 487 262 2151
Cuts at: 6809 7305 2009
Size: 496 2310
Fragments arranged by size:
2310 2151 727 580 496 487 298 262
222 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2687 3098 3251 4019 4024 1163
Size: 573 951 411 153 768 5 4745
Fragments arranged by size:
4745 951 768 573 411 153 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4572 5056 5817 6845 1540
Size: 275 186 2571 484 761 1028 2301
Fragments arranged by size:
2571 2301 1028 761 484 275 186
BsiHKAI G_wGCw'C
Cuts at: 2169 3200 3594 3735 4592 5667 5752 6823 2169
Size: 1031 394 141 857 1075 85 1071 2952
Fragments arranged by size:
2952 1075 1071 1031 857 394 141 85
BsmI GAATG_Cn'
Cuts at: 2330 2903 5117 5210 2330
Size: 573 2214 93 4726
Fragments arranged by size:
4726 2214 573 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2297 3908 4015 4021 2297
Size: 1611 107 6 5882
Fragments arranged by size:
5882 1611 107 6
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2100 2169 2548 3200 3594 3735
Size: 399 235 75 69 379 652 394 141
Cuts at: 3735 3907 4356 4554 4592 4732 4925 5667 5752
Size: 172 449 198 38 140 193 742 85
Cuts at: 5752 6823 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 652 449 399 394 379
235 198 193 172 141 140 85 75
69 38
BspEI T'CCGG_A
Cuts at: 2763 7269 2763
Size: 4506 3100
BspHI T'CATG_A
Cuts at: 5495 7225 5495
Size: 1730 5876
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2277 2922 4284 4494 4832 4961 844
Size: 1433 645 1362 210 338 129 3489
Fragments arranged by size:
3489 1433 1362 645 338 210 129
BsrBI CCG'CTC
Cuts at: 1394 1823 5493 1394
Size: 429 3670 3507
Fragments arranged by size:
3670 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3745 6101 6275 65
Size: 1183 2497 2356 174 1396
Fragments arranged by size:
2497 2356 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2069 4247 6250 6437 1700
Size: 110 259 2178 2003 187 2869
Fragments arranged by size:
2869 2178 2003 259 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7606
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7606
BssSI C'ACGA_G
Cuts at: 5666 6678 5666
Size: 1012 6594
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2431 794
Size: 1637 5969
BstBI TT'CG_AA
Cuts at: 6410 6410
Size: 7606
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7606
BstXI CCAn_nnnn'nTGG
Cuts at: 2581 2641 2992 2581
Size: 60 351 7195
Fragments arranged by size:
7195 351 60
BstYI r'GATC_y
Cuts at: 1972 2532 2971 2997 3214 3411 4633 4829 4936
Size: 560 439 26 217 197 1222 196 107
Cuts at: 4936 5303 5688 5705 7157 7243 7255 7601 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1222 560 439 385 367 346
217 197 196 107 86 26 17 12
Bsu36I CC'TnA_GG
Cuts at: 3249 4173 3249
Size: 924 6682
BtgI C'CryG_G
Cuts at: 513 1065 2949 4203 4280 4314 4368 4797 513
Size: 552 1884 1254 77 34 54 429 3322
Fragments arranged by size:
3322 1884 1254 552 429 77 54 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6867
Fragments arranged by size:
6867 683 56
BtsI GCAGTG_nn'
Cuts at: 2122 3311 3491 4002 4007 4109 4475 5122 5916
Size: 1189 180 511 5 102 366 647 794
Cuts at: 5916 5936 2122
Size: 20 3792
Fragments arranged by size:
3792 1189 794 647 511 366 180 102
20 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7606
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7571
DraI TTT'AAA
Cuts at: 4910 4910
Size: 7606
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6613 809
Size: 507 5297 1802
Fragments arranged by size:
5297 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2757 3702 3990
Size: 54 1425 50 275 18 927 945 288
Cuts at: 3990 4101 4245 4569 5053 5942 7571 8
Size: 111 144 324 484 889 1629 43
Fragments arranged by size:
1629 1425 945 927 889 484 324 288
275 144 111 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4569 5053 1537
Size: 275 2757 484 4090
Fragments arranged by size:
4090 2757 484 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2264 4409 4496 4580 5536 1353
Size: 911 2145 87 84 956 3423
Fragments arranged by size:
3423 2145 956 911 87 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2167 2486 3686 6193 6567 6713
Size: 1064 138 578 319 1200 2507 374 146
Cuts at: 6713 387
Size: 1280
Fragments arranged by size:
2507 1280 1200 1064 578 374 319 146
138
EcoICRI GAG'CTC
Cuts at: 2167 3198 3592 4590 2167
Size: 1031 394 998 5183
Fragments arranged by size:
5183 1031 998 394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2732 3014 3169 3368 3437 3516
Size: 393 599 501 282 155 199 69 79
Cuts at: 3516 3578 3620 3633 4601 4674 4697 4760 5669
Size: 62 42 13 968 73 23 63 909
Cuts at: 5669 6266 7053 7321 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 968 909 787 599 597 501 393
282 268 199 155 79 73 69 63
62 42 23 13
EcoNI CCTnn'n_nnAGG
Cuts at: 3648 3648
Size: 7606
EcoO109I rG'GnC_Cy
Cuts at: 2096 2191 2544 2545 2682 3534 3580 3653 4072
Size: 95 353 1 137 852 46 73 419
Cuts at: 4072 4140 4550 4551 4609 4728 4729 2096
Size: 68 410 1 58 119 1 4973
Fragments arranged by size:
4973 852 419 410 353 137 119 95
73 68 58 46 1 1 1
EcoRI G'AATT_C
Cuts at: 4915 5293 4915
Size: 378 7228
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3380 3380
Size: 7606
FspI TGC'GCA
Cuts at: 6112 6112
Size: 7606
HaeII r_GCGC'y
Cuts at: 1678 2033 2079 2405 3825 6753 1678
Size: 355 46 326 1420 2928 2531
Fragments arranged by size:
2928 2531 1420 355 326 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 3506 3538 3868
Size: 32 135 32 899 32 1003 32 330
Cuts at: 3868 3900 4056 4088 6247 6279 6321 6353 1373
Size: 32 156 32 2159 32 42 32 2626
Fragments arranged by size:
2626 2159 1003 899 330 156 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4950 5196 669
Size: 988 281 3012 246 3079
Fragments arranged by size:
3079 3012 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4647 748
Size: 3899 3707
HpaI GTT'AAC
Cuts at: 5196 5196
Size: 7606
KasI G'GCGC_C
Cuts at: 2075 3821 2075
Size: 1746 5860
KpnI G_GTAC'C
Cuts at: 4931 4931
Size: 7606
MfeI C'AATT_G
Cuts at: 5205 5205
Size: 7606
MluI A'CGCG_T
Cuts at: 2616 7482 2616
Size: 4866 2740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3172 3942 6720 6904 1305
Size: 1867 770 2778 184 2007
Fragments arranged by size:
2778 2007 1867 770 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2759 10
Size: 54 1425 343 927 4857
Fragments arranged by size:
4857 1425 927 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2868 3510 3627 5564 5923 6082 518
Size: 648 1702 642 117 1937 359 159 2042
Fragments arranged by size:
2042 1937 1702 648 642 359 159 117
MspA1I CmG'CkG
Cuts at: 1606 2380 3055 3193 3304 3598 3909 4282 5022
Size: 774 675 138 111 294 311 373 740
Cuts at: 5022 5699 6847 7092 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 774 740 677 675 373 311
294 245 138 111
NaeI GCC'GGC
Cuts at: 2071 2071
Size: 7606
NarI GG'CG_CC
Cuts at: 2076 3822 2076
Size: 1746 5860
NcoI C'CATG_G
Cuts at: 513 1065 2949 4314 4797 513
Size: 552 1884 1365 483 3322
Fragments arranged by size:
3322 1884 1365 552 483
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7606
NgoMIV G'CCGG_C
Cuts at: 2069 2069
Size: 7606
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7606
NotI GC'GGCC_GC
Cuts at: 5053 5053
Size: 7606
NsiI A_TGCA'T
Cuts at: 3345 3345
Size: 7606
NspI r_CATG'y
Cuts at: 1132 1229 4964 6509 1132
Size: 97 3735 1545 2229
Fragments arranged by size:
3735 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2770 3538 4051 4192 4670 1348
Size: 1422 768 513 141 478 4284
Fragments arranged by size:
4284 1422 768 513 478 141
PciI A'CATG_T
Cuts at: 6505 6505
Size: 7606
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2677 2890 3583 4612 4844 1108
Size: 1569 213 693 1029 232 3870
Fragments arranged by size:
3870 1569 1029 693 232 213
PfoI T'CCnGG_A
Cuts at: 2060 2463 2958 3072 3414 4506 2060
Size: 403 495 114 342 1092 5160
Fragments arranged by size:
5160 1092 495 403 342 114
PmeI GTTT'AAAC
Cuts at: 4910 4910
Size: 7606
PmlI CAC'GTG
Cuts at: 4327 4903 4327
Size: 576 7030
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2294 2326 5675 5707 7188 7220 2294
Size: 32 3349 32 1481 32 2680
Fragments arranged by size:
3349 2680 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2682 3580 4072 2191
Size: 491 898 492 5725
Fragments arranged by size:
5725 898 492 491
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7606
PsiI TTA'TAA
Cuts at: 2733 5176 2733
Size: 2443 5163
PspOMI G'GGCC_C
Cuts at: 2096 2544 4550 4728 2096
Size: 448 2006 178 4974
Fragments arranged by size:
4974 2006 448 178
PstI C_TGCA'G
Cuts at: 830 2370 2427 2571 2745 2784 4958 830
Size: 1540 57 144 174 39 2174 3478
Fragments arranged by size:
3478 2174 1540 174 144 57 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7606
PvuII CAG'CTG
Cuts at: 2380 3055 3193 3304 3598 2380
Size: 675 138 111 294 6388
Fragments arranged by size:
6388 675 294 138 111
SacI G_AGCT'C
Cuts at: 2169 3200 3594 4592 2169
Size: 1031 394 998 5183
Fragments arranged by size:
5183 1031 998 394
SacII CC_GC'GG
Cuts at: 4283 4283
Size: 7606
SalI G'TCGA_C
Cuts at: 1936 4948 1936
Size: 3012 4594
SanDI GG'GwC_CC
Cuts at: 2682 2682
Size: 7606
SapI GCTCTTCn'nnn_
Cuts at: 4496 4580 4496
Size: 84 7522
SbfI CC_TGCA'GG
Cuts at: 2571 4958 2571
Size: 2387 5219
ScaI AGT'ACT
Cuts at: 3954 5854 7566 3954
Size: 1900 1712 3994
Fragments arranged by size:
3994 1900 1712
SfcI C'TryA_G
Cuts at: 826 1045 2344 2366 2423 2567 2741 2780 2852
Size: 219 1299 22 57 144 174 39 72
Cuts at: 2852 4954 6089 6770 6961 826
Size: 2102 1135 681 191 1471
Fragments arranged by size:
2102 1471 1299 1135 681 219 191 174
144 72 57 39 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 3710 3710
Size: 7606
SfoI GGC'GCC
Cuts at: 2077 3823 2077
Size: 1746 5860
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7606
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7606
SmaI CCC'GGG
Cuts at: 1872 2094 4933 1872
Size: 222 2839 4545
Fragments arranged by size:
4545 2839 222
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5710 6611 6873 820
Size: 197 926 3767 901 262 1553
Fragments arranged by size:
3767 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5397 493
Size: 4904 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7606
SphI G_CATG'C
Cuts at: 1132 4964 1132
Size: 3832 3774
SspI AAT'ATT
Cuts at: 5 52 5530 5
Size: 47 5478 2081
Fragments arranged by size:
5478 2081 47
StuI AGG'CCT
Cuts at: 1517 2831 3254 3380 3476 1517
Size: 1314 423 126 96 5647
Fragments arranged by size:
5647 1314 423 126 96
StyI C'CwwG_G
Cuts at: 513 1065 2949 3387 4075 4314 4797 5045 513
Size: 552 1884 438 688 239 483 248 3074
Fragments arranged by size:
3074 1884 688 552 483 438 248 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5633 5650 5803 5988 6433 325
Size: 1255 4053 17 153 185 445 1498
Fragments arranged by size:
4053 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3952 4266 5852
Size: 274 80 33 51 1443 1975 314 1586
Cuts at: 5852 7564 96
Size: 1712 138
Fragments arranged by size:
1975 1712 1586 1443 314 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7566 7598 7566
Size: 32 7574
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7606
XbaI T'CTAG_A
Cuts at: 4942 4942
Size: 7606
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2767 3316 3943 2767
Size: 549 627 6430
Fragments arranged by size:
6430 627 549
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7606
XmaI C'CCGG_G
Cuts at: 1870 2092 4931 1870
Size: 222 2839 4545
Fragments arranged by size:
4545 2839 222
XmnI GAAnn'nnTTC
Cuts at: 3501 4226 5735 3501
Size: 725 1509 5372
Fragments arranged by size:
5372 1509 725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5414 276
Size: 53 83 186 936 3880 2468
Fragments arranged by size:
3880 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BsiWI BstZ17I DraIII EcoRV FseI FspAI
NruI PacI PspXI PsrI RsrII SexAI SrfI SwaI