Restriction map for
pFN21AE9441
(Circular) (Six-base) MAPSORT of: pFN21AE9441.seq Check: 7378 from: 1 to: 7426
pFN21AE9441 7426 bp
With 182 enzymes: SgfI *
February 2, 2012 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3672 3672
Size: 7426
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5236 278
Size: 53 83 186 936 3700 2468
Fragments arranged by size:
3700 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4769 1937
Size: 2832 4594
Acc65I G'GTAC_C
Cuts at: 4747 4747
Size: 7426
AclI AA'CG_TT
Cuts at: 1563 5553 5926 1563
Size: 3990 373 3063
Fragments arranged by size:
3990 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3116 3930 4419 5489 6873 7141 1632
Size: 1484 814 489 1070 1384 268 1917
Fragments arranged by size:
1917 1484 1384 1070 814 489 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7229
AflIII A'CryG_T
Cuts at: 1203 2386 6325 7302 1203
Size: 1183 3939 977 1327
Fragments arranged by size:
3939 1327 1183 977
AgeI A'CCGG_T
Cuts at: 6257 6257
Size: 7426
AhdI GACnn_n'nnGTC
Cuts at: 3292 6155 3292
Size: 2863 4563
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7426
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2159 1184
Size: 975 6451
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3977 4009 3977
Size: 32 7394
AlwNI CAG_nnn'CTG
Cuts at: 4682 5169 6741 4682
Size: 487 1572 5367
Fragments arranged by size:
5367 1572 487
ApaLI G'TGCA_C
Cuts at: 5483 6639 5483
Size: 1156 6270
ApoI r'AATT_y
Cuts at: 1493 2410 2747 2963 3214 4016 4735 4961 5113
Size: 917 337 216 251 802 719 226 152
Cuts at: 5113 7222 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 917 802 719 337 251 226
216 152
AseI AT'TA_AT
Cuts at: 160 1032 5980 160
Size: 872 4948 1606
Fragments arranged by size:
4948 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7426
AvaI C'yCGr_G
Cuts at: 1870 1943 4751 1870
Size: 73 2808 4545
Fragments arranged by size:
4545 2808 73
BamHI G'GATC_C
Cuts at: 4756 5123 4756
Size: 367 7059
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2499 2945 3887 4124 4747
Size: 325 217 627 712 446 942 237 623
Cuts at: 4747 6202 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 942 712 627 623 446 325
237 217
BanII G_rGCy'C
Cuts at: 1391 2130 2175 2703 4164 4745 1391
Size: 739 45 528 1461 581 4072
Fragments arranged by size:
4072 1461 739 581 528 45
BbeI G_GCGC'C
Cuts at: 4128 4128
Size: 7426
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2194 3603 928
Size: 971 295 1409 4751
Fragments arranged by size:
4751 1409 971 295
BbvCI CC'TCA_GC
Cuts at: 2096 2195 2096
Size: 99 7327
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2242 2276 5617 5651 1903
Size: 34 305 34 3341 34 3678
Fragments arranged by size:
3678 3341 305 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5318 6528 5318
Size: 1210 6216
BclI T'GATC_A
Cuts at: 1548 3681 1548
Size: 2133 5293
BfrBI ATG'CAT
Cuts at: 3254 3254
Size: 7426
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3842 4076 6037
Size: 107 122 71 631 605 2170 234 1961
Cuts at: 6037 136
Size: 1525
Fragments arranged by size:
2170 1961 1525 631 605 234 122 107
71
BglII A'GATC_T
Cuts at: 2897 3021 3076 7421 2897
Size: 124 55 4345 2902
Fragments arranged by size:
4345 2902 124 55
BlpI GC'TnA_GC
Cuts at: 3197 4843 3197
Size: 1646 5780
Bme1580I G_kGCm'C
Cuts at: 1790 2466 4072 5487 6643 1790
Size: 676 1606 1415 1156 2573
Fragments arranged by size:
2573 1606 1415 1156 676
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7426
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2259 3102 3333
Size: 416 53 83 399 24 842 843 231
Cuts at: 3333 4644 5157 6115 442
Size: 1311 513 958 1753
Fragments arranged by size:
1753 1311 958 843 842 513 416 399
231 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2617 3014 3033 3814 4004 4292 4609
Size: 1061 317 397 19 781 190 288 317
Cuts at: 4609 6086 1239
Size: 1477 2579
Fragments arranged by size:
2579 1477 1061 781 397 317 317 288
190 19
Bpu10I CC'TnA_GC
Cuts at: 2096 2147 2195 2096
Size: 51 48 7327
Fragments arranged by size:
7327 51 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3392 3537 4584 5551 6416 6714 3392
Size: 145 1047 967 865 298 4104
Fragments arranged by size:
4104 1047 967 865 298 145
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4260 6089 882
Size: 617 2761 1829 2219
Fragments arranged by size:
2761 2219 1829 617
BsaAI yAC'GTr
Cuts at: 493 1204 2389 5217 493
Size: 711 1185 2828 2702
Fragments arranged by size:
2828 2702 1185 711
BsaBI GATnn'nnATC
Cuts at: 7420 7420
Size: 7426
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4125 5233 5615 6250
Size: 53 83 186 936 2592 1108 382 635
Cuts at: 6250 275
Size: 1451
Fragments arranged by size:
2592 1451 1108 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5859 6257 6531 6678 7089 5859
Size: 398 274 147 411 6196
Fragments arranged by size:
6196 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3048 3078 3977 4007 4015
Size: 30 165 30 1645 30 899 30 8
Cuts at: 4015 4045 1178
Size: 30 4559
Fragments arranged by size:
4559 1645 899 165 30 30 30 30
30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 4357 1186
Size: 1111 2060 4255
Fragments arranged by size:
4255 2060 1111
BseYI C'CCAG_C
Cuts at: 4105 4163 6629 7125 4105
Size: 58 2466 496 4406
Fragments arranged by size:
4406 2466 496 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3641 1163
Size: 573 1905 4948
Fragments arranged by size:
4948 1905 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2064 3209 4876 5637 6665 1540
Size: 275 186 63 1145 1667 761 1028 2301
Fragments arranged by size:
2301 1667 1145 1028 761 275 186 63
BsiHKAI G_wGCw'C
Cuts at: 2031 3019 5487 5572 6643 2031
Size: 988 2468 85 1071 2814
Fragments arranged by size:
2814 2468 1071 988 85
BsmI GAATG_Cn'
Cuts at: 2330 2488 3469 4937 5030 2330
Size: 158 981 1468 93 4726
Fragments arranged by size:
4726 1468 981 158 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2130 2175 2466 2703 3019 4072
Size: 399 241 99 45 291 237 316 1053
Cuts at: 4072 4164 4745 5487 5572 6643 1391
Size: 92 581 742 85 1071 2174
Fragments arranged by size:
2174 1071 1053 742 581 399 316 291
241 237 99 92 85 45
BspEI T'CCGG_A
Cuts at: 7089 7089
Size: 7426
BspHI T'CATG_A
Cuts at: 4218 5315 7045 4218
Size: 1097 1730 4599
Fragments arranged by size:
4599 1730 1097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3672 4650 4781 844
Size: 2828 978 131 3489
Fragments arranged by size:
3489 2828 978 131
BsrBI CCG'CTC
Cuts at: 1394 1823 5313 1394
Size: 429 3490 3507
Fragments arranged by size:
3507 3490 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2119 2564 4227 4305 5921 6095 65
Size: 1183 871 445 1663 78 1616 174 1396
Fragments arranged by size:
1663 1616 1396 1183 871 445 174 78
BsrFI r'CCGG_y
Cuts at: 1700 1810 6070 6257 1700
Size: 110 4260 187 2869
Fragments arranged by size:
4260 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3765 96
Size: 3669 3757
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7426
BssSI C'ACGA_G
Cuts at: 5486 6498 5486
Size: 1012 6414
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2413 794
Size: 1619 5807
BstBI TT'CG_AA
Cuts at: 6230 6230
Size: 7426
BstEII G'GTnAC_C
Cuts at: 1184 4609 1184
Size: 3425 4001
BstXI CCAn_nnnn'nTGG
Cuts at: 2581 2891 3923 4171 2581
Size: 310 1032 248 5836
Fragments arranged by size:
5836 1032 310 248
BstYI r'GATC_y
Cuts at: 1972 2897 3021 3076 3755 4492 4756 5123 5508
Size: 925 124 55 679 737 264 367 385
Cuts at: 5508 5525 6977 7063 7075 7421 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 925 737 679 385 367 346
264 124 86 55 17 12
Bsu36I CC'TnA_GG
Cuts at: 2495 2495
Size: 7426
BtgI C'CryG_G
Cuts at: 513 1065 2004 4086 4170 513
Size: 552 939 2082 84 3769
Fragments arranged by size:
3769 2082 939 552 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3784 487
Size: 683 56 2558 4129
Fragments arranged by size:
4129 2558 683 56
BtsI GCAGTG_nn'
Cuts at: 2754 3629 3813 4942 5736 5756 2754
Size: 875 184 1129 794 20 4424
Fragments arranged by size:
4424 1129 875 794 184 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7426
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7391
DraI TTT'AAA
Cuts at: 2727 4730 2727
Size: 2003 5423
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6433 809
Size: 507 5117 1802
Fragments arranged by size:
5117 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4089 4873 5762
Size: 54 1425 50 275 18 2259 784 889
Cuts at: 5762 7391 8
Size: 1629 43
Fragments arranged by size:
2259 1629 1425 889 784 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4873 1537
Size: 275 3061 4090
Fragments arranged by size:
4090 3061 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3036 4325 5356 1353
Size: 1683 1289 1031 3423
Fragments arranged by size:
3423 1683 1289 1031
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6013 6387 6533 387
Size: 1064 138 4424 374 146 1280
Fragments arranged by size:
4424 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2617 3014 3033 3116 3814 3930
Size: 393 668 317 397 19 83 698 116
Cuts at: 3930 4004 4292 4419 4609 5489 6086 6873 7141
Size: 74 288 127 190 880 597 787 268
Cuts at: 7141 1239
Size: 1524
Fragments arranged by size:
1524 880 787 698 668 597 397 393
317 288 268 190 127 116 83 74
19
EcoNI CCTnn'n_nnAGG
Cuts at: 3636 3636
Size: 7426
EcoO109I rG'GnC_Cy
Cuts at: 3641 3641
Size: 7426
EcoRI G'AATT_C
Cuts at: 2747 4735 5113 2747
Size: 1988 378 5060
Fragments arranged by size:
5060 1988 378
EcoRV GAT'ATC
Cuts at: 3384 3384
Size: 7426
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 7426
FspI TGC'GCA
Cuts at: 5932 5932
Size: 7426
HaeII r_GCGC'y
Cuts at: 1678 4128 6573 1678
Size: 2450 2445 2531
Fragments arranged by size:
2531 2450 2445
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3006 3013 3038 3045 3278
Size: 32 135 32 1434 7 25 7 233
Cuts at: 3278 3310 3466 3498 4678 4710 6067 6099 6141
Size: 32 156 32 1180 32 1357 32 42
Cuts at: 6141 6173 1373
Size: 32 2626
Fragments arranged by size:
2626 1434 1357 1180 233 156 135 42
32 32 32 32 32 32 32 25
7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2526 3178 4770 5016 669
Size: 988 281 588 652 1592 246 3079
Fragments arranged by size:
3079 1592 988 652 588 281 246
HindIII A'AGCT_T
Cuts at: 748 3128 4546 748
Size: 2380 1418 3628
Fragments arranged by size:
3628 2380 1418
HpaI GTT'AAC
Cuts at: 2526 5016 2526
Size: 2490 4936
KasI G'GCGC_C
Cuts at: 4124 4124
Size: 7426
KpnI G_GTAC'C
Cuts at: 4751 4751
Size: 7426
MfeI C'AATT_G
Cuts at: 5025 5025
Size: 7426
MluI A'CGCG_T
Cuts at: 7302 7302
Size: 7426
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2187 2214 2799 3321 6540 6724 1305
Size: 882 27 585 522 3219 184 2007
Fragments arranged by size:
3219 2007 882 585 522 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5604
Fragments arranged by size:
5604 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2868 3249 5384 5743 5902 518
Size: 648 1702 381 2135 359 159 2042
Fragments arranged by size:
2135 2042 1702 648 381 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2245 4842 5519 6667 6912 1606
Size: 450 189 2597 677 1148 245 2120
Fragments arranged by size:
2597 2120 1148 677 450 245 189
NarI GG'CG_CC
Cuts at: 4125 4125
Size: 7426
NcoI C'CATG_G
Cuts at: 513 1065 2004 4086 4170 513
Size: 552 939 2082 84 3769
Fragments arranged by size:
3769 2082 939 552 84
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7426
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7426
NotI GC'GGCC_GC
Cuts at: 4873 4873
Size: 7426
NsiI A_TGCA'T
Cuts at: 3256 3256
Size: 7426
NspI r_CATG'y
Cuts at: 1132 1229 3817 4446 4784 6329 1132
Size: 97 2588 629 338 1545 2229
Fragments arranged by size:
2588 2229 1545 629 338 97
PasI CC'CwG_GG
Cuts at: 1348 2677 1348
Size: 1329 6097
PciI A'CATG_T
Cuts at: 6325 6325
Size: 7426
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2677 1108
Size: 1569 5857
PfoI T'CCnGG_A
Cuts at: 2277 2277
Size: 7426
PmeI GTTT'AAAC
Cuts at: 4730 4730
Size: 7426
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2952 3977 4009 5495 5527 7008 7040 2920
Size: 32 1025 32 1486 32 1481 32 3306
Fragments arranged by size:
3306 1486 1481 1025 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7426
PsiI TTA'TAA
Cuts at: 4996 4996
Size: 7426
PstI C_TGCA'G
Cuts at: 830 2055 2370 2409 4778 830
Size: 1225 315 39 2369 3478
Fragments arranged by size:
3478 2369 1225 315 39
PvuI CG_AT'CG
Cuts at: 2001 3209 2001
Size: 1208 6218
PvuII CAG'CTG
Cuts at: 2056 2245 2056
Size: 189 7237
SalI G'TCGA_C
Cuts at: 1936 4768 1936
Size: 2832 4594
SapI GCTCTTCn'nnn_
Cuts at: 4325 4325
Size: 7426
SbfI CC_TGCA'GG
Cuts at: 4778 4778
Size: 7426
ScaI AGT'ACT
Cuts at: 5674 7386 5674
Size: 1712 5714
SfcI C'TryA_G
Cuts at: 826 1045 2051 2366 2405 4774 5909 6590 6781
Size: 219 1006 315 39 2369 1135 681 191
Cuts at: 6781 826
Size: 1471
Fragments arranged by size:
2369 1471 1135 1006 681 315 219 191
39
SfoI GGC'GCC
Cuts at: 4126 4126
Size: 7426
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7426
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7426
SmaI CCC'GGG
Cuts at: 1872 4753 1872
Size: 2881 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3407 3552 4599 5530 6431 6693
Size: 197 926 1464 145 1047 931 901 262
Cuts at: 6693 820
Size: 1553
Fragments arranged by size:
1553 1464 1047 931 926 901 262 197
145
SnaBI TAC'GTA
Cuts at: 493 5217 493
Size: 4724 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7426
SphI G_CATG'C
Cuts at: 1132 4784 1132
Size: 3652 3774
SspI AAT'ATT
Cuts at: 5 52 4007 4279 5350 5
Size: 47 3955 272 1071 2081
Fragments arranged by size:
3955 2081 1071 272 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7426
StyI C'CwwG_G
Cuts at: 513 1065 2004 2322 2580 4086 4170 4605 4865
Size: 552 939 318 258 1506 84 435 260
Cuts at: 4865 513
Size: 3074
Fragments arranged by size:
3074 1506 939 552 435 318 260 258
84
SwaI ATTT'AAAT
Cuts at: 2727 2727
Size: 7426
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4097 4557 5453 5470 5623 5808 6253
Size: 1255 2517 460 896 17 153 185 445
Cuts at: 6253 325
Size: 1498
Fragments arranged by size:
2517 1498 1255 896 460 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3765 5672 7384
Size: 274 80 33 51 1443 1788 1907 1712
Cuts at: 7384 96
Size: 138
Fragments arranged by size:
1907 1788 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3481 3513 7386 7418 3481
Size: 32 3873 32 3489
Fragments arranged by size:
3873 3489 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7426
XbaI T'CTAG_A
Cuts at: 4762 4762
Size: 7426
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3709 3838 4177 3709
Size: 129 339 6958
Fragments arranged by size:
6958 339 129
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7426
XmaI C'CCGG_G
Cuts at: 1870 4751 1870
Size: 2881 4545
XmnI GAAnn'nnTTC
Cuts at: 3396 5555 3396
Size: 2159 5267
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5234 276
Size: 53 83 186 936 3700 2468
Fragments arranged by size:
3700 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BplI BsiWI BsmBI
BstZ17I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SrfI