Restriction map for
pFN21AE9437
(Circular) (Six-base) MAPSORT of: pFN21AE9437.seq Check: 7349 from: 1 to: 5794
pFN21AE9437 5794 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2169 2169
Size: 5794
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3604 278
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3137 1937
Size: 1200 4594
Acc65I G'GTAC_C
Cuts at: 3115 3115
Size: 5794
AclI AA'CG_TT
Cuts at: 1563 3921 4294 1563
Size: 2358 373 3063
Fragments arranged by size:
3063 2358 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2963 3035 3857 5241 5509 1632
Size: 1331 72 822 1384 268 1917
Fragments arranged by size:
1917 1384 1331 822 268 72
AfeI AGC'GCT
Cuts at: 2104 2684 2104
Size: 580 5214
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5597
AflIII A'CryG_T
Cuts at: 1203 3091 4693 5670 1203
Size: 1888 1602 977 1327
Fragments arranged by size:
1888 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4625 4625
Size: 5794
AhdI GACnn_n'nnGTC
Cuts at: 4523 4523
Size: 5794
AleI CACnn'nnGTG
Cuts at: 1166 2670 1166
Size: 1504 4290
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2449 1184
Size: 1265 4529
AlwNI CAG_nnn'CTG
Cuts at: 2320 2407 2423 2610 3013 3537 5109 2320
Size: 87 16 187 403 524 1572 3005
Fragments arranged by size:
3005 1572 524 403 187 87 16
ApaI G_GGCC'C
Cuts at: 2012 2012
Size: 5794
ApaLI G'TGCA_C
Cuts at: 2588 2930 3002 3851 5007 2588
Size: 342 72 849 1156 3375
Fragments arranged by size:
3375 1156 849 342 72
ApoI r'AATT_y
Cuts at: 1493 3103 3329 3481 5590 1493
Size: 1610 226 152 2109 1697
Fragments arranged by size:
2109 1697 1610 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4348 160
Size: 872 3316 1606
Fragments arranged by size:
3316 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
AvaI C'yCGr_G
Cuts at: 1870 1943 2118 2489 3119 1870
Size: 73 175 371 630 4545
Fragments arranged by size:
4545 630 371 175 73
BamHI G'GATC_C
Cuts at: 3124 3491 3124
Size: 367 5427
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2227 2706 3115 4570 618
Size: 325 217 627 440 479 409 1455 1842
Fragments arranged by size:
1842 1455 627 479 440 409 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2112 2799 3113 1391
Size: 621 100 687 314 4072
Fragments arranged by size:
4072 687 621 314 100
BbeI G_GCGC'C
Cuts at: 2231 2710 2231
Size: 479 5315
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2027 928
Size: 971 128 4695
Fragments arranged by size:
4695 971 128
BbvCI CC'TCA_GC
Cuts at: 2837 2837
Size: 5794
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2443 2477 3985 4019 1903
Size: 34 506 34 1508 34 3678
Fragments arranged by size:
3678 1508 506 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4896 3686
Size: 1210 4584
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4405 136
Size: 107 122 71 631 605 2733 1525
Fragments arranged by size:
2733 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5789 5789
Size: 5794
BlpI GC'TnA_GC
Cuts at: 2106 2288 2303 3211 2106
Size: 182 15 908 4689
Fragments arranged by size:
4689 908 182 15
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2592 2805 2934 3006 3855 5011 1790
Size: 222 580 213 129 72 849 1156 2573
Fragments arranged by size:
2573 1156 849 580 222 213 129 72
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2664 2818 3525
Size: 416 53 83 399 24 1247 154 707
Cuts at: 3525 4483 442
Size: 958 1753
Fragments arranged by size:
1753 1247 958 707 416 399 154 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2567 4454 1239
Size: 1328 1887 2579
Fragments arranged by size:
2579 1887 1328
Bpu10I CC'TnA_GC
Cuts at: 2496 2837 2496
Size: 341 5453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3919 4784 5082 3919
Size: 865 298 4631
Fragments arranged by size:
4631 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4457 882
Size: 617 2958 2219
Fragments arranged by size:
2958 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2883 3585 493
Size: 711 1679 702 2702
Fragments arranged by size:
2702 1679 711 702
BsaBI GATnn'nnATC
Cuts at: 5788 5788
Size: 5794
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2130 2228 2273 2353
Size: 53 83 186 936 597 98 45 80
Cuts at: 2353 2707 3601 3983 4618 275
Size: 354 894 382 635 1451
Fragments arranged by size:
1451 936 894 635 597 382 354 186
98 83 80 53 45
BsaWI w'CCGG_w
Cuts at: 4227 4625 4899 5046 5457 4227
Size: 398 274 147 411 4564
Fragments arranged by size:
4564 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5569
Fragments arranged by size:
5569 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5794
BseYI C'CCAG_C
Cuts at: 2152 2383 2959 3031 4997 5493 2152
Size: 231 576 72 1966 496 2453
Fragments arranged by size:
2453 1966 576 496 231 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2409 2648 1163
Size: 573 673 239 4309
Fragments arranged by size:
4309 673 573 239
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2363 2903 3244 4005 5033 1540
Size: 275 186 362 540 341 761 1028 2301
Fragments arranged by size:
2301 1028 761 540 362 341 275 186
BsiHKAI G_wGCw'C
Cuts at: 2112 2568 2592 2799 2934 3006 3855 3940 5011
Size: 456 24 207 135 72 849 85 1071
Cuts at: 5011 2112
Size: 2895
Fragments arranged by size:
2895 1071 849 456 207 135 85 72
24
BsmI GAATG_Cn'
Cuts at: 3305 3398 3305
Size: 93 5701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2112 2568 2592 2799 2805 2934
Size: 399 222 100 456 24 207 6 129
Cuts at: 2934 3006 3113 3855 3940 5011 1391
Size: 72 107 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 456 399 222 207 129
107 100 85 72 24 6
BspEI T'CCGG_A
Cuts at: 5457 5457
Size: 5794
BspHI T'CATG_A
Cuts at: 2778 3683 5413 2778
Size: 905 1730 3159
Fragments arranged by size:
3159 1730 905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 2670 3149 844
Size: 1325 501 479 3489
Fragments arranged by size:
3489 1325 501 479
BsrBI CCG'CTC
Cuts at: 1394 1823 3681 1394
Size: 429 1858 3507
Fragments arranged by size:
3507 1858 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2736 4289 4463 65
Size: 1183 1488 1553 174 1396
Fragments arranged by size:
1553 1488 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2372 2898 2921 2934 2993 4438 4625
Size: 110 562 526 23 13 59 1445 187
Cuts at: 4625 1700
Size: 2869
Fragments arranged by size:
2869 1445 562 526 187 110 59 23
13
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5794
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5794
BssSI C'ACGA_G
Cuts at: 2437 2798 3854 4866 2437
Size: 361 1056 1012 3365
Fragments arranged by size:
3365 1056 1012 361
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2610 2938 3010 794
Size: 1816 328 72 3578
Fragments arranged by size:
3578 1816 328 72
BstBI TT'CG_AA
Cuts at: 4598 4598
Size: 5794
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5794
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 2249 2077
Size: 172 5622
BstYI r'GATC_y
Cuts at: 1972 3124 3491 3876 3893 5345 5431 5443 5789
Size: 1152 367 385 17 1452 86 12 346
Cuts at: 5789 1972
Size: 1977
Fragments arranged by size:
1977 1452 1152 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2125 2125
Size: 5794
BtgI C'CryG_G
Cuts at: 513 1065 2004 2055 2538 513
Size: 552 939 51 483 3769
Fragments arranged by size:
3769 939 552 483 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5055
Fragments arranged by size:
5055 683 56
BtsI GCAGTG_nn'
Cuts at: 2331 2336 2608 3310 4104 4124 2331
Size: 5 272 702 794 20 4001
Fragments arranged by size:
4001 794 702 272 20 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5759
DraI TTT'AAA
Cuts at: 3098 3098
Size: 5794
DraIII CAC_nnn'GTG
Cuts at: 2596 2596
Size: 5794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4801 809
Size: 507 3485 1802
Fragments arranged by size:
3485 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2868 2900
Size: 54 1425 50 275 18 222 816 32
Cuts at: 2900 2919 2991 3241 4130 5759 8
Size: 19 72 250 889 1629 43
Fragments arranged by size:
1629 1425 889 816 275 250 222 72
54 50 43 32 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2900 3241 1537
Size: 275 1088 341 4090
Fragments arranged by size:
4090 1088 341 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2939 3011 3724 1353
Size: 1586 72 713 3423
Fragments arranged by size:
3423 1586 713 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2230 2807 2885 2897 4381 4755
Size: 1064 138 641 577 78 12 1484 374
Cuts at: 4755 4901 387
Size: 146 1280
Fragments arranged by size:
1484 1280 1064 641 577 374 146 138
78 12
EcoICRI GAG'CTC
Cuts at: 2110 2797 2110
Size: 687 5107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2567 2963 3035 3857 4454 5241 5509
Size: 393 935 396 72 822 597 787 268
Cuts at: 5509 1239
Size: 1524
Fragments arranged by size:
1524 935 822 787 597 396 393 268
72
EcoO109I rG'GnC_Cy
Cuts at: 2009 2009
Size: 5794
EcoRI G'AATT_C
Cuts at: 3103 3481 3103
Size: 378 5416
FspI TGC'GCA
Cuts at: 4300 4300
Size: 5794
HaeII r_GCGC'y
Cuts at: 1678 2024 2063 2106 2177 2231 2288 2303 2424
Size: 346 39 43 71 54 57 15 121
Cuts at: 2424 2460 2645 2686 2710 4941 1678
Size: 36 185 41 24 2231 2531
Fragments arranged by size:
2531 2231 346 185 121 71 57 54
43 41 39 36 24 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4435 4467 4509 4541 1373
Size: 32 135 32 2863 32 42 32 2626
Fragments arranged by size:
2863 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3138 3384 669
Size: 988 281 1200 246 3079
Fragments arranged by size:
3079 1200 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5794
HpaI GTT'AAC
Cuts at: 3384 3384
Size: 5794
KasI G'GCGC_C
Cuts at: 2227 2706 2227
Size: 479 5315
KpnI G_GTAC'C
Cuts at: 3119 3119
Size: 5794
MfeI C'AATT_G
Cuts at: 3393 3393
Size: 5794
MluI A'CGCG_T
Cuts at: 5670 5670
Size: 5794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 2301 4908 5092 1305
Size: 789 207 2607 184 2007
Fragments arranged by size:
2607 2007 789 207 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3972
Fragments arranged by size:
3972 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2670 3752 4111 4270 518
Size: 648 1504 1082 359 159 2042
Fragments arranged by size:
2042 1504 1082 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2557 3010 3210 3887 5035 5280 1606
Size: 951 453 200 677 1148 245 2120
Fragments arranged by size:
2120 1148 951 677 453 245 200
NaeI GCC'GGC
Cuts at: 2374 2900 2923 2995 2374
Size: 526 23 72 5173
Fragments arranged by size:
5173 526 72 23
NarI GG'CG_CC
Cuts at: 2228 2707 2228
Size: 479 5315
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4303
Fragments arranged by size:
4303 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5794
NgoMIV G'CCGG_C
Cuts at: 2372 2898 2921 2993 2372
Size: 526 23 72 5173
Fragments arranged by size:
5173 526 72 23
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5794
NotI GC'GGCC_GC
Cuts at: 3241 3241
Size: 5794
NspI r_CATG'y
Cuts at: 1132 1229 2185 2241 3152 4697 1132
Size: 97 956 56 911 1545 2229
Fragments arranged by size:
2229 1545 956 911 97 56
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5794
PciI A'CATG_T
Cuts at: 4693 4693
Size: 5794
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 2539 2620 2917 1108
Size: 1212 219 81 297 3985
Fragments arranged by size:
3985 1212 297 219 81
PfoI T'CCnGG_A
Cuts at: 2256 2256
Size: 5794
PmeI GTTT'AAAC
Cuts at: 3098 3098
Size: 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3863 3895 5376 5408 3863
Size: 32 1481 32 4249
Fragments arranged by size:
4249 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5794
PsiI TTA'TAA
Cuts at: 3364 3364
Size: 5794
PspOMI G'GGCC_C
Cuts at: 2008 2008
Size: 5794
PspXI vC'TCGA_Gb
Cuts at: 2118 2118
Size: 5794
PstI C_TGCA'G
Cuts at: 830 3084 3146 830
Size: 2254 62 3478
Fragments arranged by size:
3478 2254 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5794
PvuII CAG'CTG
Cuts at: 3010 3010
Size: 5794
SacI G_AGCT'C
Cuts at: 2112 2799 2112
Size: 687 5107
SalI G'TCGA_C
Cuts at: 1936 3136 1936
Size: 1200 4594
SbfI CC_TGCA'GG
Cuts at: 3146 3146
Size: 5794
ScaI AGT'ACT
Cuts at: 4042 5754 4042
Size: 1712 4082
SfcI C'TryA_G
Cuts at: 826 1045 2098 2449 2854 3080 3142 4277 4958
Size: 219 1053 351 405 226 62 1135 681
Cuts at: 4958 5149 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1053 681 405 351 226 219
191 62
SfoI GGC'GCC
Cuts at: 2229 2708 2229
Size: 479 5315
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5794
SmaI CCC'GGG
Cuts at: 1872 3121 1872
Size: 1249 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2118 3898 4799 5061 820
Size: 197 926 175 1780 901 262 1553
Fragments arranged by size:
1780 1553 926 901 262 197 175
SnaBI TAC'GTA
Cuts at: 493 3585 493
Size: 3092 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5794
SphI G_CATG'C
Cuts at: 1132 2241 3152 1132
Size: 1109 911 3774
Fragments arranged by size:
3774 1109 911
SspI AAT'ATT
Cuts at: 5 52 3718 5
Size: 47 3666 2081
Fragments arranged by size:
3666 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5794
StyI C'CwwG_G
Cuts at: 513 1065 2004 3233 513
Size: 552 939 1229 3074
Fragments arranged by size:
3074 1229 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2984 3056 3821 3838 3991 4176 4621
Size: 1255 1404 72 765 17 153 185 445
Cuts at: 4621 325
Size: 1498
Fragments arranged by size:
1498 1404 1255 765 445 185 153 72
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4040 5752 96
Size: 274 80 33 51 1443 2063 1712 138
Fragments arranged by size:
2063 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5754 5786 5754
Size: 32 5762
Tth111I GACn'n_nGTC
Cuts at: 1316 2221 2627 1316
Size: 905 406 4483
Fragments arranged by size:
4483 905 406
XbaI T'CTAG_A
Cuts at: 3130 3130
Size: 5794
XhoI C'TCGA_G
Cuts at: 1943 2118 1943
Size: 175 5619
XmaI C'CCGG_G
Cuts at: 1870 3119 1870
Size: 1249 4545
XmnI GAAnn'nnTTC
Cuts at: 3923 3923
Size: 5794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3602 276
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PpuMI PsrI RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI