Restriction map for
pFN21AE9436
(Circular) (Six-base) MAPSORT of: pFN21AE9436.seq Check: 2887 from: 1 to: 7756
pFN21AE9436 7756 bp
With 182 enzymes: SgfI *
March 28, 2012 11:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2164 3565 2164
Size: 1401 6355
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5566 278
Size: 53 83 186 936 4030 2468
Fragments arranged by size:
4030 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5099 1937
Size: 3162 4594
Acc65I G'GTAC_C
Cuts at: 4237 5077 4237
Size: 840 6916
AclI AA'CG_TT
Cuts at: 1563 5883 6256 1563
Size: 4320 373 3063
Fragments arranged by size:
4320 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2067 2693 2907 3407 5819 7203 7471 1632
Size: 435 626 214 500 2412 1384 268 1917
Fragments arranged by size:
2412 1917 1384 626 500 435 268 214
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7559
AflIII A'CryG_T
Cuts at: 1203 4959 6655 7632 1203
Size: 3756 1696 977 1327
Fragments arranged by size:
3756 1696 1327 977
AgeI A'CCGG_T
Cuts at: 6587 6587
Size: 7756
AhdI GACnn_n'nnGTC
Cuts at: 3327 6485 3327
Size: 3158 4598
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7756
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7756
AlwNI CAG_nnn'CTG
Cuts at: 4097 5499 7071 4097
Size: 1402 1572 4782
Fragments arranged by size:
4782 1572 1402
ApaLI G'TGCA_C
Cuts at: 5813 6969 5813
Size: 1156 6600
ApoI r'AATT_y
Cuts at: 1493 4214 4307 4714 4789 5065 5291 5443 7552
Size: 2721 93 407 75 276 226 152 2109
Cuts at: 7552 1493
Size: 1697
Fragments arranged by size:
2721 2109 1697 407 276 226 152 93
75
AseI AT'TA_AT
Cuts at: 160 1032 6310 160
Size: 872 5278 1606
Fragments arranged by size:
5278 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7756
AvaI C'yCGr_G
Cuts at: 1870 1943 2799 3685 5081 1870
Size: 73 856 886 1396 4545
Fragments arranged by size:
4545 1396 886 856 73
BamHI G'GATC_C
Cuts at: 2725 3571 4295 5086 5453 2725
Size: 846 724 791 367 5028
Fragments arranged by size:
5028 846 791 724 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2438 2794 3011 4237 5077
Size: 325 217 627 651 356 217 1226 840
Cuts at: 5077 6532 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1226 840 651 627 356 325
217 217
BanII G_rGCy'C
Cuts at: 1391 2487 4474 5075 1391
Size: 1096 1987 601 4072
Fragments arranged by size:
4072 1987 1096 601
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2084 4030 928
Size: 971 185 1946 4654
Fragments arranged by size:
4654 1946 971 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5947 5981 1903
Size: 34 4010 34 3678
Fragments arranged by size:
4010 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2664 5648 6858 2664
Size: 2984 1210 3562
Fragments arranged by size:
3562 2984 1210
BclI T'GATC_A
Cuts at: 1548 3207 3360 3597 1548
Size: 1659 153 237 5707
Fragments arranged by size:
5707 1659 237 153
BfrBI ATG'CAT
Cuts at: 2303 2600 2303
Size: 297 7459
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 3260 6367
Size: 107 122 71 631 605 365 1223 3107
Cuts at: 6367 136
Size: 1525
Fragments arranged by size:
3107 1525 1223 631 605 365 122 107
71
BglII A'GATC_T
Cuts at: 7751 7751
Size: 7756
BlpI GC'TnA_GC
Cuts at: 2844 5173 2844
Size: 2329 5427
Bme1580I G_kGCm'C
Cuts at: 1790 5817 6973 1790
Size: 4027 1156 2573
Fragments arranged by size:
4027 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7756
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2783 4475 4538
Size: 416 53 83 399 24 1366 1692 63
Cuts at: 4538 5487 6445 442
Size: 949 958 1753
Fragments arranged by size:
1753 1692 1366 958 949 416 399 83
63 53 24
BmtI G_CTAG'C
Cuts at: 1056 3524 1056
Size: 2468 5288
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3607 3607
Size: 7756
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2187 2469 2835 3203 3602 4744 6416 1239
Size: 948 282 366 368 399 1142 1672 2579
Fragments arranged by size:
2579 1672 1142 948 399 368 366 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3155 5881 6746 7044 3155
Size: 2726 865 298 3867
Fragments arranged by size:
3867 2726 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4129 4906 6419 882
Size: 617 2630 777 1513 2219
Fragments arranged by size:
2630 2219 1513 777 617
BsaAI yAC'GTr
Cuts at: 493 1204 2756 5547 493
Size: 711 1552 2791 2702
Fragments arranged by size:
2791 2702 1552 711
BsaBI GATnn'nnATC
Cuts at: 7750 7750
Size: 7756
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2310 5563 5945 6580
Size: 53 83 186 936 777 3253 382 635
Cuts at: 6580 275
Size: 1451
Fragments arranged by size:
3253 1451 936 777 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3972 4324 6189 6587 6861 7008 7419 3972
Size: 352 1865 398 274 147 411 4309
Fragments arranged by size:
4309 1865 411 398 352 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3004 3034 3430 3460 1178
Size: 30 165 30 1601 30 396 30 5474
Fragments arranged by size:
5474 1601 396 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2179 3762 1186
Size: 993 1583 5180
Fragments arranged by size:
5180 1583 993
BseYI C'CCAG_C
Cuts at: 2916 4473 6959 7455 2916
Size: 1557 2486 496 3217
Fragments arranged by size:
3217 2486 1557 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7183
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5206 5967 6995 1540
Size: 275 186 3205 761 1028 2301
Fragments arranged by size:
3205 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2487 5817 5902 6973 2487
Size: 3330 85 1071 3270
Fragments arranged by size:
3330 3270 1071 85
BsmI GAATG_Cn'
Cuts at: 2602 3376 4151 5267 5360 2602
Size: 774 775 1116 93 4998
Fragments arranged by size:
4998 1116 775 774 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2487 4474 5075 5817 5902 6973 1391
Size: 399 697 1987 601 742 85 1071 2174
Fragments arranged by size:
2174 1987 1071 742 697 601 399 85
BspEI T'CCGG_A
Cuts at: 3972 7419 3972
Size: 3447 4309
BspHI T'CATG_A
Cuts at: 3508 5645 7375 3508
Size: 2137 1730 3889
Fragments arranged by size:
3889 2137 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2164 2727 3565 5111 844
Size: 1320 563 838 1546 3489
Fragments arranged by size:
3489 1546 1320 838 563
BsrBI CCG'CTC
Cuts at: 1394 1823 5643 1394
Size: 429 3820 3507
Fragments arranged by size:
3820 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2359 4676 4993 6251 6425 65
Size: 1183 1111 2317 317 1258 174 1396
Fragments arranged by size:
2317 1396 1258 1183 1111 317 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6400 6587 1700
Size: 110 4590 187 2869
Fragments arranged by size:
4590 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2564 96
Size: 2468 5288
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7756
BssSI C'ACGA_G
Cuts at: 4051 5816 6828 4051
Size: 1765 1012 4979
Fragments arranged by size:
4979 1765 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7756
BstBI TT'CG_AA
Cuts at: 6560 6560
Size: 7756
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7756
BstXI CCAn_nnnn'nTGG
Cuts at: 2756 2791 2756
Size: 35 7721
BstYI r'GATC_y
Cuts at: 1972 2725 3571 3969 4295 5086 5453 5838 5855
Size: 753 846 398 326 791 367 385 17
Cuts at: 5855 7307 7393 7405 7751 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 846 791 753 398 385 367
346 326 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4499 4499
Size: 7756
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6265
Fragments arranged by size:
6265 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3468 487
Size: 683 56 2242 4775
Fragments arranged by size:
4775 2242 683 56
BtsI GCAGTG_nn'
Cuts at: 2030 2650 4829 5272 6066 6086 2030
Size: 620 2179 443 794 20 3700
Fragments arranged by size:
3700 2179 794 620 443 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7756
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7721
DraI TTT'AAA
Cuts at: 4749 5060 4749
Size: 311 7445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6763 809
Size: 507 5447 1802
Fragments arranged by size:
5447 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 4647 5203
Size: 54 1425 50 275 18 199 2618 556
Cuts at: 5203 6092 7721 8
Size: 889 1629 43
Fragments arranged by size:
2618 1629 1425 889 556 275 199 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5203 1537
Size: 275 3391 4090
Fragments arranged by size:
4090 3391 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2688 2986 3480 4789 5686 1353
Size: 1335 298 494 1309 897 3423
Fragments arranged by size:
3423 1335 1309 897 494 298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6343 6717 6863 387
Size: 1064 138 4754 374 146 1280
Fragments arranged by size:
4754 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2485 2485
Size: 7756
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2067 2187 2469 2693 2835 2907 3203
Size: 393 435 120 282 224 142 72 296
Cuts at: 3203 3407 3602 4744 5819 6416 7203 7471 1239
Size: 204 195 1142 1075 597 787 268 1524
Fragments arranged by size:
1524 1142 1075 787 597 435 393 296
282 268 224 204 195 142 120 72
EcoO109I rG'GnC_Cy
Cuts at: 2821 2821
Size: 7756
EcoRI G'AATT_C
Cuts at: 5065 5443 5065
Size: 378 7378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2532 2532
Size: 7756
FspI TGC'GCA
Cuts at: 6262 6262
Size: 7756
HaeII r_GCGC'y
Cuts at: 1678 6903 1678
Size: 5225 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2364 2396 3192 3224 3313
Size: 32 135 32 792 32 796 32 89
Cuts at: 3313 3345 4666 4698 6397 6429 6471 6503 1373
Size: 32 1321 32 1699 32 42 32 2626
Fragments arranged by size:
2626 1699 1321 796 792 135 89 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3073 3112 3702 4573 5100 5346
Size: 988 281 1135 39 590 871 527 246
Cuts at: 5346 669
Size: 3079
Fragments arranged by size:
3079 1135 988 871 590 527 281 246
39
HindIII A'AGCT_T
Cuts at: 748 2242 2705 3826 5032 748
Size: 1494 463 1121 1206 3472
Fragments arranged by size:
3472 1494 1206 1121 463
HpaI GTT'AAC
Cuts at: 3702 4573 5346 3702
Size: 871 773 6112
Fragments arranged by size:
6112 871 773
KpnI G_GTAC'C
Cuts at: 4241 5081 4241
Size: 840 6916
MfeI C'AATT_G
Cuts at: 5355 5355
Size: 7756
MluI A'CGCG_T
Cuts at: 7632 7632
Size: 7756
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2679 6870 7054 1305
Size: 1374 4191 184 2007
Fragments arranged by size:
4191 2007 1374 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2031 4649 10
Size: 54 1425 343 199 2618 3117
Fragments arranged by size:
3117 2618 1425 343 199 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2754 2789 2954 4091 4132 4136 4370
Size: 648 1588 35 165 1137 41 4 234
Cuts at: 4370 5714 6073 6232 518
Size: 1344 359 159 2042
Fragments arranged by size:
2042 1588 1344 1137 648 359 234 165
159 41 35 4
MspA1I CmG'CkG
Cuts at: 1606 3304 4006 4244 5172 5849 6997 7242 1606
Size: 1698 702 238 928 677 1148 245 2120
Fragments arranged by size:
2120 1698 1148 928 702 677 245 238
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6265
Fragments arranged by size:
6265 939 552
NdeI CA'TA_TG
Cuts at: 387 4158 387
Size: 3771 3985
NheI G'CTAG_C
Cuts at: 1052 3520 1052
Size: 2468 5288
NotI GC'GGCC_GC
Cuts at: 5203 5203
Size: 7756
NsiI A_TGCA'T
Cuts at: 2305 2602 2305
Size: 297 7459
NspI r_CATG'y
Cuts at: 1132 1229 2604 2959 3250 4417 4963 5114 6659
Size: 97 1375 355 291 1167 546 151 1545
Cuts at: 6659 1132
Size: 2229
Fragments arranged by size:
2229 1545 1375 1167 546 355 291 151
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7756
PciI A'CATG_T
Cuts at: 4959 6655 4959
Size: 1696 6060
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7756
PfoI T'CCnGG_A
Cuts at: 4044 4044
Size: 7756
PmeI GTTT'AAAC
Cuts at: 5060 5060
Size: 7756
PmlI CAC'GTG
Cuts at: 2756 2756
Size: 7756
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5825 5857 7338 7370 5825
Size: 32 1481 32 6211
Fragments arranged by size:
6211 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2821 2821
Size: 7756
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7756
PsiI TTA'TAA
Cuts at: 2525 5326 2525
Size: 2801 4955
PstI C_TGCA'G
Cuts at: 830 2724 5108 830
Size: 1894 2384 3478
Fragments arranged by size:
3478 2384 1894
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7756
PvuII CAG'CTG
Cuts at: 3304 4006 4244 3304
Size: 702 238 6816
Fragments arranged by size:
6816 702 238
SacI G_AGCT'C
Cuts at: 2487 2487
Size: 7756
SalI G'TCGA_C
Cuts at: 1936 5098 1936
Size: 3162 4594
SapI GCTCTTCn'nnn_
Cuts at: 4789 4789
Size: 7756
SbfI CC_TGCA'GG
Cuts at: 2724 5108 2724
Size: 2384 5372
ScaI AGT'ACT
Cuts at: 6004 7716 6004
Size: 1712 6044
SexAI A'CCwGG_T
Cuts at: 4032 4032
Size: 7756
SfcI C'TryA_G
Cuts at: 826 1045 2283 2535 2720 3299 3811 4884 5104
Size: 219 1238 252 185 579 512 1073 220
Cuts at: 5104 6239 6920 7111 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1238 1135 1073 681 579 512 252
220 219 191 185
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7756
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7756
SmaI CCC'GGG
Cuts at: 1872 5083 1872
Size: 3211 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3134 5860 6761 7023 820
Size: 197 926 1191 2726 901 262 1553
Fragments arranged by size:
2726 1553 1191 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5547 493
Size: 5054 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7756
SphI G_CATG'C
Cuts at: 1132 5114 1132
Size: 3982 3774
SspI AAT'ATT
Cuts at: 5 52 5680 5
Size: 47 5628 2081
Fragments arranged by size:
5628 2081 47
StuI AGG'CCT
Cuts at: 1517 4080 1517
Size: 2563 5193
StyI C'CwwG_G
Cuts at: 513 1065 2004 3171 4768 5195 513
Size: 552 939 1167 1597 427 3074
Fragments arranged by size:
3074 1597 1167 939 552 427
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5783 5800 5953 6138 6583 325
Size: 1255 4203 17 153 185 445 1498
Fragments arranged by size:
4203 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2564 4855 6002
Size: 274 80 33 51 1443 587 2291 1147
Cuts at: 6002 7714 96
Size: 1712 138
Fragments arranged by size:
2291 1712 1443 1147 587 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3004 3036 3078 3110 7716 7748 3004
Size: 32 42 32 4606 32 3012
Fragments arranged by size:
4606 3012 42 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3107 1316
Size: 1791 5965
XbaI T'CTAG_A
Cuts at: 5092 5092
Size: 7756
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7756
XmaI C'CCGG_G
Cuts at: 1870 5081 1870
Size: 3211 4545
XmnI GAAnn'nnTTC
Cuts at: 2706 4879 5017 5885 2706
Size: 2173 138 868 4577
Fragments arranged by size:
4577 2173 868 138
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5564 276
Size: 53 83 186 936 4030 2468
Fragments arranged by size:
4030 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI XcmI