Restriction map for
pFN21AE9434
(Circular) (Six-base) MAPSORT of: pFN21AE9434.seq Check: 8738 from: 1 to: 5716
pFN21AE9434 5716 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3059 1937
Size: 1122 4594
Acc65I G'GTAC_C
Cuts at: 3037 3037
Size: 5716
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3779 5163 5431 1632
Size: 2147 1384 268 1917
Fragments arranged by size:
2147 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 2391 4615 5592 1203
Size: 1188 2224 977 1327
Fragments arranged by size:
2224 1327 1188 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 2670 1166
Size: 1504 4212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 3459 5031 3459
Size: 1572 4144
ApaLI G'TGCA_C
Cuts at: 2167 2470 3773 4929 2167
Size: 303 1303 1156 2954
Fragments arranged by size:
2954 1303 1156 303
ApoI r'AATT_y
Cuts at: 1493 3025 3251 3403 5512 1493
Size: 1532 226 152 2109 1697
Fragments arranged by size:
2109 1697 1532 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 3041 1870
Size: 73 1098 4545
Fragments arranged by size:
4545 1098 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2888 2921 2888
Size: 33 5683
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2525 2809 3037 4492
Size: 325 217 627 335 403 284 228 1455
Cuts at: 4492 618
Size: 1842
Fragments arranged by size:
1842 1455 627 403 335 325 284 228
217
BanII G_rGCy'C
Cuts at: 1391 2799 3035 1391
Size: 1408 236 4072
Fragments arranged by size:
4072 1408 236
BbeI G_GCGC'C
Cuts at: 2126 2529 2126
Size: 403 5313
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2323 2509 928
Size: 971 424 186 4135
Fragments arranged by size:
4135 971 424 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2602 2636 3907 3941 1903
Size: 34 665 34 1271 34 3678
Fragments arranged by size:
3678 1271 665 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 2628 2778 1548
Size: 1080 150 4486
Fragments arranged by size:
4486 1080 150
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2976 4327 136
Size: 107 122 71 631 605 1304 1351 1525
Fragments arranged by size:
1525 1351 1304 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 2288 2303 2339 3133 2288
Size: 15 36 794 4871
Fragments arranged by size:
4871 794 36 15
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2171 2474 2814 3777 4933 1790
Size: 258 123 303 340 963 1156 2573
Fragments arranged by size:
2573 1156 963 340 303 258 123
BmgBI CAC'GTC
Cuts at: 1318 2626 1318
Size: 1308 4408
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2664 2836 3447
Size: 416 53 83 399 24 1247 172 611
Cuts at: 3447 4405 442
Size: 958 1753
Fragments arranged by size:
1753 1247 958 611 416 399 172 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5716
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2711 4376 1239
Size: 1472 1665 2579
Fragments arranged by size:
2579 1665 1472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2576 3841 4706 5004 2567
Size: 9 1265 865 298 3279
Fragments arranged by size:
3279 1265 865 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4379 882
Size: 617 2880 2219
Fragments arranged by size:
2880 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2151 2394 2577 3507 493
Size: 711 947 243 183 930 2702
Fragments arranged by size:
2702 947 930 711 243 183
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 2123 2202 2526
Size: 53 83 186 936 522 68 79 324
Cuts at: 2526 3523 3905 4540 275
Size: 997 382 635 1451
Fragments arranged by size:
1451 997 936 635 522 382 324 186
83 79 68 53
BsaWI w'CCGG_w
Cuts at: 4149 4547 4821 4968 5379 4149
Size: 398 274 147 411 4486
Fragments arranged by size:
4486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2936 2966 1178
Size: 30 165 30 1533 30 3928
Fragments arranged by size:
3928 1533 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2708 2953 1186
Size: 1522 245 3949
Fragments arranged by size:
3949 1522 245
BseYI C'CCAG_C
Cuts at: 2316 2416 4919 5415 2316
Size: 100 2503 496 2617
Fragments arranged by size:
2617 2503 496 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2384 2500 2557 2648 2927 1163
Size: 573 345 303 116 57 91 279 3952
Fragments arranged by size:
3952 573 345 303 279 116 91 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2233 2314 3166 3927 4955 1540
Size: 275 186 232 81 852 761 1028 2301
Fragments arranged by size:
2301 1028 852 761 275 232 186 81
BsiHKAI G_wGCw'C
Cuts at: 2171 2474 2568 2592 2799 3777 3862 4933 2171
Size: 303 94 24 207 978 85 1071 2954
Fragments arranged by size:
2954 1071 978 303 207 94 85 24
BsmI GAATG_Cn'
Cuts at: 3227 3320 3227
Size: 93 5623
BsmBI CGTCTCn'nnnn_
Cuts at: 2062 2062
Size: 5716
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2171 2474 2568 2592 2799 2814
Size: 399 258 123 303 94 24 207 15
Cuts at: 2814 3035 3777 3862 4933 1391
Size: 221 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 303 258 221 207
123 94 85 24 15
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 3605 5335 3605
Size: 1730 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 2670 3071 844
Size: 1325 501 401 3489
Fragments arranged by size:
3489 1325 501 401
BsrBI CCG'CTC
Cuts at: 1394 1823 2188 3603 1394
Size: 429 365 1415 3507
Fragments arranged by size:
3507 1415 429 365
BsrDI GCAATG_nn'
Cuts at: 65 1248 2608 4211 4385 65
Size: 1183 1360 1603 174 1396
Fragments arranged by size:
1603 1396 1360 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2125 2198 2221 2484 3004 4360 4547
Size: 110 315 73 23 263 520 1356 187
Cuts at: 4547 1700
Size: 2869
Fragments arranged by size:
2869 1356 520 315 263 187 110 73
23
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5716
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 2011 2467 2645 3776 4788 2011
Size: 456 178 1131 1012 2939
Fragments arranged by size:
2939 1131 1012 456 178
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2478 2916 794
Size: 1684 438 3594
Fragments arranged by size:
3594 1684 438
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5716
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 2791 2848 2966 2077
Size: 714 57 118 4827
Fragments arranged by size:
4827 714 118 57
BstYI r'GATC_y
Cuts at: 1972 3046 3413 3798 3815 5267 5353 5365 5711
Size: 1074 367 385 17 1452 86 12 346
Cuts at: 5711 1972
Size: 1977
Fragments arranged by size:
1977 1452 1074 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2172 2325 2805 513
Size: 552 1107 153 480 3424
Fragments arranged by size:
3424 1107 552 480 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 3232 4026 4046 3232
Size: 794 20 4902
Fragments arranged by size:
4902 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DraIII CAC_nnn'GTG
Cuts at: 2478 2478
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2295 2322
Size: 54 1425 50 275 18 366 99 27
Cuts at: 2322 2489 3163 4052 5681 8
Size: 167 674 889 1629 43
Fragments arranged by size:
1629 1425 889 674 366 275 167 99
54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3163 1537
Size: 275 1351 4090
Fragments arranged by size:
4090 1351 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2138 2804 3646 1353
Size: 785 666 842 3423
Fragments arranged by size:
3423 842 785 666
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2102 4303 4677 4823 387
Size: 1064 138 513 2201 374 146 1280
Fragments arranged by size:
2201 1280 1064 513 374 146 138
EcoICRI GAG'CTC
Cuts at: 2797 2797
Size: 5716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2711 3779 4376 5163 5431 1239
Size: 393 1079 1068 597 787 268 1524
Fragments arranged by size:
1524 1079 1068 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2748 2748
Size: 5716
EcoRI G'AATT_C
Cuts at: 3025 3403 3025
Size: 378 5338
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 2126 2132 2378 2503 2529 4863 1678
Size: 448 6 246 125 26 2334 2531
Fragments arranged by size:
2531 2334 448 246 125 26 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2940 2972 4357 4389 4431
Size: 32 135 32 1368 32 1385 32 42
Cuts at: 4431 4463 1373
Size: 32 2626
Fragments arranged by size:
2626 1385 1368 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2179 3060 3306 669
Size: 988 281 241 881 246 3079
Fragments arranged by size:
3079 988 881 281 246 241
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5716
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KasI G'GCGC_C
Cuts at: 2122 2525 2122
Size: 403 5313
KpnI G_GTAC'C
Cuts at: 3041 3041
Size: 5716
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 2895 4830 5014 1305
Size: 789 801 1935 184 2007
Fragments arranged by size:
2007 1935 801 789 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3894
Fragments arranged by size:
3894 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2624 2670 3674 4033 4192 518
Size: 648 1458 46 1004 359 159 2042
Fragments arranged by size:
2042 1458 1004 648 359 159 46
MspA1I CmG'CkG
Cuts at: 1606 2215 2316 2338 2458 3132 3809 4957 5202
Size: 609 101 22 120 674 677 1148 245
Cuts at: 5202 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 674 609 245 120 101
22
NaeI GCC'GGC
Cuts at: 2127 2200 2223 2486 2127
Size: 73 23 263 5357
Fragments arranged by size:
5357 263 73 23
NarI GG'CG_CC
Cuts at: 2123 2526 2123
Size: 403 5313
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5164
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NgoMIV G'CCGG_C
Cuts at: 2125 2198 2221 2484 2125
Size: 73 23 263 5357
Fragments arranged by size:
5357 263 73 23
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5716
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 2185 3074 4619 1132
Size: 97 956 889 1545 2229
Fragments arranged by size:
2229 1545 956 889 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5716
PciI A'CATG_T
Cuts at: 4615 4615
Size: 5716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5716
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PmlI CAC'GTG
Cuts at: 2151 2394 2151
Size: 243 5473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2748 2748
Size: 5716
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PstI C_TGCA'G
Cuts at: 830 3068 830
Size: 2238 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
PvuII CAG'CTG
Cuts at: 2458 2458
Size: 5716
SacI G_AGCT'C
Cuts at: 2799 2799
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SapI GCTCTTCn'nnn_
Cuts at: 2138 2804 2138
Size: 666 5050
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 3964 5676 3964
Size: 1712 4004
SfcI C'TryA_G
Cuts at: 826 1045 3064 4199 4880 5071 826
Size: 219 2019 1135 681 191 1471
Fragments arranged by size:
2019 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2124 2527 2124
Size: 403 5313
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 2125 1700
Size: 425 5291
SmaI CCC'GGG
Cuts at: 1872 3043 1872
Size: 1171 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2582 2591 3820 4721 4983 820
Size: 197 926 639 9 1229 901 262 1553
Fragments arranged by size:
1553 1229 926 901 639 262 197 9
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5716
StyI C'CwwG_G
Cuts at: 513 1065 3155 513
Size: 552 2090 3074
Fragments arranged by size:
3074 2090 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2308 3743 3760 3913 4098 4543 325
Size: 1255 728 1435 17 153 185 445 1498
Fragments arranged by size:
1498 1435 1255 728 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2883 3962 5674
Size: 274 80 33 51 1443 906 1079 1712
Cuts at: 5674 96
Size: 138
Fragments arranged by size:
1712 1443 1079 906 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2936 2968 5676 5708 2936
Size: 32 2708 32 2944
Fragments arranged by size:
2944 2708 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2290 2290
Size: 5716
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 3041 1870
Size: 1171 4545
XmnI GAAnn'nnTTC
Cuts at: 3845 3845
Size: 5716
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SrfI SwaI