Restriction map for
pFN21AE9433
(Circular) (Six-base) MAPSORT of: pFN21AE9433.seq Check: 9390 from: 1 to: 6250
pFN21AE9433 6250 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2585 3236 2585
Size: 651 5599
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4060 278
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2865 3593 1937
Size: 928 728 4594
Fragments arranged by size:
4594 928 728
Acc65I G'GTAC_C
Cuts at: 3571 3571
Size: 6250
AclI AA'CG_TT
Cuts at: 1563 4377 4750 1563
Size: 2814 373 3063
Fragments arranged by size:
3063 2814 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4313 5697 5965 1632
Size: 2681 1384 268 1917
Fragments arranged by size:
2681 1917 1384 268
AfeI AGC'GCT
Cuts at: 2736 2736
Size: 6250
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6053
AflIII A'CryG_T
Cuts at: 1203 2055 5149 6126 1203
Size: 852 3094 977 1327
Fragments arranged by size:
3094 1327 977 852
AgeI A'CCGG_T
Cuts at: 3024 5081 3024
Size: 2057 4193
AhdI GACnn_n'nnGTC
Cuts at: 4979 4979
Size: 6250
AleI CACnn'nnGTG
Cuts at: 1166 2141 2213 1166
Size: 975 72 5203
Fragments arranged by size:
5203 975 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6250
AlwNI CAG_nnn'CTG
Cuts at: 3112 3320 3993 5565 3112
Size: 208 673 1572 3797
Fragments arranged by size:
3797 1572 673 208
ApaI G_GGCC'C
Cuts at: 2509 2698 2509
Size: 189 6061
ApaLI G'TGCA_C
Cuts at: 4307 5463 4307
Size: 1156 5094
ApoI r'AATT_y
Cuts at: 1493 2366 3559 3785 3937 6046 1493
Size: 873 1193 226 152 2109 1697
Fragments arranged by size:
2109 1697 1193 873 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4804 160
Size: 872 3772 1606
Fragments arranged by size:
3772 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2260 3097 3575 1870
Size: 73 153 164 837 478 4545
Fragments arranged by size:
4545 837 478 164 153 73
BamHI G'GATC_C
Cuts at: 3580 3947 3580
Size: 367 5883
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2063 3307 3475 3571 5026
Size: 325 217 627 276 1244 168 96 1455
Cuts at: 5026 618
Size: 1842
Fragments arranged by size:
1842 1455 1244 627 325 276 217 168
96
BanII G_rGCy'C
Cuts at: 1391 2509 2698 3569 1391
Size: 1118 189 871 4072
Fragments arranged by size:
4072 1118 871 189
BbeI G_GCGC'C
Cuts at: 2067 2067
Size: 6250
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2818 2893 928
Size: 971 919 75 4285
Fragments arranged by size:
4285 971 919 75
BbvCI CC'TCA_GC
Cuts at: 2306 2402 3069 2306
Size: 96 667 5487
Fragments arranged by size:
5487 667 96
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2077 2111 4441 4475 1903
Size: 34 140 34 2330 34 3678
Fragments arranged by size:
3678 2330 140 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3307 4142 5352 3307
Size: 835 1210 4205
Fragments arranged by size:
4205 1210 835
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6250
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4861 136
Size: 107 122 71 631 605 3189 1525
Fragments arranged by size:
3189 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3141 6245 3141
Size: 3104 3146
BlpI GC'TnA_GC
Cuts at: 3193 3667 3193
Size: 474 5776
Bme1580I G_kGCm'C
Cuts at: 1790 2292 2509 2698 2847 3480 4311 5467 1790
Size: 502 217 189 149 633 831 1156 2573
Fragments arranged by size:
2573 1156 831 633 502 217 189 149
BmgBI CAC'GTC
Cuts at: 1318 2571 1318
Size: 1253 4997
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3981 4939 442
Size: 416 53 83 399 24 2564 958 1753
Fragments arranged by size:
2564 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2474 2611 3134 3254 4910 1239
Size: 1235 137 523 120 1656 2579
Fragments arranged by size:
2579 1656 1235 523 137 120
Bpu10I CC'TnA_GC
Cuts at: 2306 2402 3069 3278 2306
Size: 96 667 209 5278
Fragments arranged by size:
5278 667 209 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4375 5240 5538 4375
Size: 865 298 5087
Fragments arranged by size:
5087 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3398 4913 882
Size: 617 1899 1515 2219
Fragments arranged by size:
2219 1899 1515 617
BsaAI yAC'GTr
Cuts at: 493 1204 4041 493
Size: 711 2837 2702
Fragments arranged by size:
2837 2702 711
BsaBI GATnn'nnATC
Cuts at: 2498 2684 6244 2498
Size: 186 3560 2504
Fragments arranged by size:
3560 2504 186
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 4057 4439 5074
Size: 53 83 186 936 531 1993 382 635
Cuts at: 5074 275
Size: 1451
Fragments arranged by size:
1993 1451 936 635 531 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2739 3024 4683 5081 5355 5502 5913 2739
Size: 285 1659 398 274 147 411 3076
Fragments arranged by size:
3076 1659 411 398 285 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6025
Fragments arranged by size:
6025 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3378 1186
Size: 2192 4058
BseYI C'CCAG_C
Cuts at: 2778 3086 5453 5949 2778
Size: 308 2367 496 3079
Fragments arranged by size:
3079 2367 496 308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2165 2563 2780 1163
Size: 573 429 398 217 4633
Fragments arranged by size:
4633 573 429 398 217
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3700 4461 5489 1540
Size: 275 186 1699 761 1028 2301
Fragments arranged by size:
2301 1699 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2880 3284 4311 4396 5467 2880
Size: 404 1027 85 1071 3663
Fragments arranged by size:
3663 1071 1027 404 85
BsmI GAATG_Cn'
Cuts at: 3761 3854 3761
Size: 93 6157
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2292 2509 2698 2847 2880 3284 3480
Size: 399 502 217 189 149 33 404 196
Cuts at: 3480 3569 4311 4396 5467 1391
Size: 89 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 502 404 399 217 196
189 149 89 85 33
BspEI T'CCGG_A
Cuts at: 2739 5913 2739
Size: 3174 3076
BspHI T'CATG_A
Cuts at: 4139 5869 4139
Size: 1730 4520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2137 2585 3236 3605 844
Size: 1293 448 651 369 3489
Fragments arranged by size:
3489 1293 651 448 369
BsrBI CCG'CTC
Cuts at: 1394 1823 3499 4137 1394
Size: 429 1676 638 3507
Fragments arranged by size:
3507 1676 638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4745 4919 65
Size: 1183 3497 174 1396
Fragments arranged by size:
3497 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3024 3310 4894 5081 1700
Size: 110 1214 286 1584 187 2869
Fragments arranged by size:
2869 1584 1214 286 187 110
BsrGI T'GTAC_A
Cuts at: 96 2496 96
Size: 2400 3850
BssHII G'CGCG_C
Cuts at: 1925 2091 1925
Size: 166 6084
BssSI C'ACGA_G
Cuts at: 4310 5322 4310
Size: 1012 5238
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6250
BstBI TT'CG_AA
Cuts at: 5054 5054
Size: 6250
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6250
BstXI CCAn_nnnn'nTGG
Cuts at: 2821 2821
Size: 6250
BstYI r'GATC_y
Cuts at: 1972 2025 2334 3141 3580 3947 4332 4349 5801
Size: 53 309 807 439 367 385 17 1452
Cuts at: 5801 5887 5899 6245 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 807 439 385 367 346 309
86 53 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2640 2814 513
Size: 552 939 636 174 3949
Fragments arranged by size:
3949 939 636 552 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5511
Fragments arranged by size:
5511 683 56
BtsI GCAGTG_nn'
Cuts at: 2520 2913 3766 4560 4580 2520
Size: 393 853 794 20 4190
Fragments arranged by size:
4190 853 794 393 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6250
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2629 2664 529
Size: 35 2065 35 4115
Fragments arranged by size:
4115 2065 35 35
DraI TTT'AAA
Cuts at: 3554 3554
Size: 6250
DraIII CAC_nnn'GTG
Cuts at: 2614 2614
Size: 6250
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2897 5257 809
Size: 507 1581 2360 1802
Fragments arranged by size:
2360 1802 1581 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2520 3697 4586
Size: 54 1425 50 275 18 690 1177 889
Cuts at: 4586 6215 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1177 889 690 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3697 1537
Size: 275 1885 4090
Fragments arranged by size:
4090 1885 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2354 2678 3162 3450 4180 1353
Size: 1001 324 484 288 730 3423
Fragments arranged by size:
3423 1001 730 484 324 288
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4837 5211 5357 387
Size: 1064 138 3248 374 146 1280
Fragments arranged by size:
3248 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2474 2611 3134 3254 4313 4910 5697
Size: 393 842 137 523 120 1059 597 787
Cuts at: 5697 5965 1239
Size: 268 1524
Fragments arranged by size:
1524 1059 842 787 597 523 393 268
137 120
EcoO109I rG'GnC_Cy
Cuts at: 2695 3003 2695
Size: 308 5942
EcoRI G'AATT_C
Cuts at: 3559 3937 3559
Size: 378 5872
FspI TGC'GCA
Cuts at: 4756 4756
Size: 6250
HaeII r_GCGC'y
Cuts at: 1678 2067 2738 5397 1678
Size: 389 671 2659 2531
Fragments arranged by size:
2659 2531 671 389
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2462 2494 3281 3313 4891
Size: 32 135 32 890 32 787 32 1578
Cuts at: 4891 4923 4965 4997 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1578 890 787 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2199 3594 3840 669
Size: 988 281 261 1395 246 3079
Fragments arranged by size:
3079 1395 988 281 261 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6250
HpaI GTT'AAC
Cuts at: 3840 3840
Size: 6250
KasI G'GCGC_C
Cuts at: 2063 2063
Size: 6250
KpnI G_GTAC'C
Cuts at: 3575 3575
Size: 6250
MfeI C'AATT_G
Cuts at: 3849 3849
Size: 6250
MluI A'CGCG_T
Cuts at: 2055 6126 2055
Size: 4071 2179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2713 5364 5548 1305
Size: 1408 2651 184 2007
Fragments arranged by size:
2651 2007 1408 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2522 10
Size: 54 1425 343 690 3738
Fragments arranged by size:
3738 1425 690 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2213 2819 4208 4567 4726 518
Size: 648 975 72 606 1389 359 159 2042
Fragments arranged by size:
2042 1389 975 648 606 359 159 72
MspA1I CmG'CkG
Cuts at: 1606 2119 2782 3197 3666 4343 5491 5736 1606
Size: 513 663 415 469 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 663 513 469 415 245
NarI GG'CG_CC
Cuts at: 2064 2064
Size: 6250
NcoI C'CATG_G
Cuts at: 513 1065 2004 2814 513
Size: 552 939 810 3949
Fragments arranged by size:
3949 939 810 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6250
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6250
NotI GC'GGCC_GC
Cuts at: 3697 3697
Size: 6250
NspI r_CATG'y
Cuts at: 1132 1229 3608 5153 1132
Size: 97 2379 1545 2229
Fragments arranged by size:
2379 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6250
PciI A'CATG_T
Cuts at: 5149 5149
Size: 6250
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2515 3112 1108
Size: 1407 597 4246
Fragments arranged by size:
4246 1407 597
PfoI T'CCnGG_A
Cuts at: 3111 3111
Size: 6250
PmeI GTTT'AAAC
Cuts at: 3554 3554
Size: 6250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2744 2776 4319 4351 5832 5864 2744
Size: 32 1543 32 1481 32 3130
Fragments arranged by size:
3130 1543 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3003 3003
Size: 6250
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6250
PsiI TTA'TAA
Cuts at: 3820 3820
Size: 6250
PspOMI G'GGCC_C
Cuts at: 2505 2694 2505
Size: 189 6061
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2114 2146 3075 3107 2114
Size: 32 929 32 5257
Fragments arranged by size:
5257 929 32 32
PstI C_TGCA'G
Cuts at: 830 3360 3602 830
Size: 2530 242 3478
Fragments arranged by size:
3478 2530 242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6250
PvuII CAG'CTG
Cuts at: 2119 3197 2119
Size: 1078 5172
SalI G'TCGA_C
Cuts at: 1936 3592 1936
Size: 1656 4594
SanDI GG'GwC_CC
Cuts at: 3003 3003
Size: 6250
SapI GCTCTTCn'nnn_
Cuts at: 2354 3450 2354
Size: 1096 5154
SbfI CC_TGCA'GG
Cuts at: 3602 3602
Size: 6250
ScaI AGT'ACT
Cuts at: 4498 6210 4498
Size: 1712 4538
SexAI A'CCwGG_T
Cuts at: 2610 2610
Size: 6250
SfcI C'TryA_G
Cuts at: 826 1045 3356 3598 4733 5414 5605 826
Size: 219 2311 242 1135 681 191 1471
Fragments arranged by size:
2311 1471 1135 681 242 219 191
SfoI GGC'GCC
Cuts at: 2065 2065
Size: 6250
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6250
SmaI CCC'GGG
Cuts at: 1872 3577 1872
Size: 1705 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4354 5255 5517 820
Size: 197 926 2411 901 262 1553
Fragments arranged by size:
2411 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4041 493
Size: 3548 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6250
SphI G_CATG'C
Cuts at: 1132 3608 1132
Size: 2476 3774
SspI AAT'ATT
Cuts at: 5 52 4174 5
Size: 47 4122 2081
Fragments arranged by size:
4122 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6250
StyI C'CwwG_G
Cuts at: 513 1065 2004 2601 2814 3210 3438 3480 3689
Size: 552 939 597 213 396 228 42 209
Cuts at: 3689 513
Size: 3074
Fragments arranged by size:
3074 939 597 552 396 228 213 209
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4277 4294 4447 4632 5077 325
Size: 1255 2697 17 153 185 445 1498
Fragments arranged by size:
2697 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 3050 3183
Size: 274 80 33 51 1443 519 554 133
Cuts at: 3183 4496 6208 96
Size: 1313 1712 138
Fragments arranged by size:
1712 1443 1313 554 519 274 138 133
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 6210 6242 2042
Size: 32 4136 32 2050
Fragments arranged by size:
4136 2050 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6250
XbaI T'CTAG_A
Cuts at: 3586 3586
Size: 6250
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6250
XmaI C'CCGG_G
Cuts at: 1870 3575 1870
Size: 1705 4545
XmnI GAAnn'nnTTC
Cuts at: 4379 4379
Size: 6250
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4058 276
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII
SacI SacII SfiI SrfI SwaI XcmI