Restriction map for
pFN21AE9432
(Circular) (Six-base) MAPSORT of: pFN21AE9432.seq Check: 773 from: 1 to: 7147
pFN21AE9432 7147 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3217 3217
Size: 7147
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3234 4957 278
Size: 53 83 186 936 1698 1723 2468
Fragments arranged by size:
2468 1723 1698 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4490 1937
Size: 2553 4594
Acc65I G'GTAC_C
Cuts at: 4022 4468 4022
Size: 446 6701
AclI AA'CG_TT
Cuts at: 1563 5274 5647 1563
Size: 3711 373 3063
Fragments arranged by size:
3711 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2960 3327 3635 3730 3984 5210 6594 6862
Size: 1328 367 308 95 254 1226 1384 268
Cuts at: 6862 1632
Size: 1917
Fragments arranged by size:
1917 1384 1328 1226 367 308 268 254
95
AfeI AGC'GCT
Cuts at: 2308 2308
Size: 7147
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6950
AflIII A'CryG_T
Cuts at: 1203 2619 3910 6046 7023 1203
Size: 1416 1291 2136 977 1327
Fragments arranged by size:
2136 1416 1327 1291 977
AgeI A'CCGG_T
Cuts at: 5978 5978
Size: 7147
AhdI GACnn_n'nnGTC
Cuts at: 5876 5876
Size: 7147
AleI CACnn'nnGTG
Cuts at: 1166 2649 4442 1166
Size: 1483 1793 3871
Fragments arranged by size:
3871 1793 1483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2720 1184
Size: 1536 5611
AlwNI CAG_nnn'CTG
Cuts at: 2059 2500 4346 4890 6462 2059
Size: 441 1846 544 1572 2744
Fragments arranged by size:
2744 1846 1572 544 441
ApaI G_GGCC'C
Cuts at: 3351 3683 3829 4120 4396 3351
Size: 332 146 291 276 6102
Fragments arranged by size:
6102 332 291 276 146
ApaLI G'TGCA_C
Cuts at: 3175 3337 5204 6360 3175
Size: 162 1867 1156 3962
Fragments arranged by size:
3962 1867 1156 162
ApoI r'AATT_y
Cuts at: 1493 4456 4682 4834 6943 1493
Size: 2963 226 152 2109 1697
Fragments arranged by size:
2963 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5701 160
Size: 872 4669 1606
Fragments arranged by size:
4669 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7147
AvaI C'yCGr_G
Cuts at: 1870 1943 3055 3324 4058 4472 1870
Size: 73 1112 269 734 414 4545
Fragments arranged by size:
4545 1112 734 414 269 73
BamHI G'GATC_C
Cuts at: 4477 4844 4477
Size: 367 6780
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2720 4022 4468 5923 618
Size: 325 217 627 933 1302 446 1455 1842
Fragments arranged by size:
1842 1455 1302 933 627 446 325 217
BanII G_rGCy'C
Cuts at: 1391 2348 3351 3606 3683 3829 4059 4120 4313
Size: 957 1003 255 77 146 230 61 193
Cuts at: 4313 4396 4466 1391
Size: 83 70 4072
Fragments arranged by size:
4072 1003 957 255 230 193 146 83
77 70 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3976 4209 928
Size: 971 2077 233 3866
Fragments arranged by size:
3866 2077 971 233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5338 5372 1903
Size: 34 3401 34 3678
Fragments arranged by size:
3678 3401 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5039 6249 5039
Size: 1210 5937
BclI T'GATC_A
Cuts at: 1548 3445 1548
Size: 1897 5250
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2729 2742 5758
Size: 107 122 71 631 605 1057 13 3016
Cuts at: 5758 136
Size: 1525
Fragments arranged by size:
3016 1525 1057 631 605 122 107 71
13
BglII A'GATC_T
Cuts at: 3667 7142 3667
Size: 3475 3672
BlpI GC'TnA_GC
Cuts at: 3819 4564 3819
Size: 745 6402
Bme1580I G_kGCm'C
Cuts at: 1790 3179 3341 3351 3683 3829 3898 4120 4197
Size: 1389 162 10 332 146 69 222 77
Cuts at: 4197 4396 5208 6364 1790
Size: 199 812 1156 2573
Fragments arranged by size:
2573 1389 1156 812 332 222 199 162
146 77 69 10
BmgBI CAC'GTC
Cuts at: 1318 2550 2620 1318
Size: 1232 70 5845
Fragments arranged by size:
5845 1232 70
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2291 3022 3328
Size: 416 53 83 399 24 874 731 306
Cuts at: 3328 4184 4371 4878 5836 442
Size: 856 187 507 958 1753
Fragments arranged by size:
1753 958 874 856 731 507 416 399
306 187 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7147
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 7147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2564 2904 3376 3608 4049 5807 1239
Size: 1325 340 472 232 441 1758 2579
Fragments arranged by size:
2579 1758 1325 472 441 340 232
Bpu10I CC'TnA_GC
Cuts at: 3270 4353 3270
Size: 1083 6064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3392 3993 5272 6137 6435 3392
Size: 601 1279 865 298 4104
Fragments arranged by size:
4104 1279 865 601 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2062 3504 5810 882
Size: 617 563 1442 2306 2219
Fragments arranged by size:
2306 2219 1442 617 563
BsaAI yAC'GTr
Cuts at: 493 1204 4938 493
Size: 711 3734 2702
Fragments arranged by size:
3734 2702 711
BsaBI GATnn'nnATC
Cuts at: 7141 7141
Size: 7147
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3231 4954 5336 5971
Size: 53 83 186 936 1698 1723 382 635
Cuts at: 5971 275
Size: 1451
Fragments arranged by size:
1723 1698 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5580 5978 6252 6399 6810 5580
Size: 398 274 147 411 5917
Fragments arranged by size:
5917 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3501 3531 1178
Size: 30 165 30 2098 30 4794
Fragments arranged by size:
4794 2098 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3053 3056 3077 3343 3428 3522 3614 3817
Size: 1867 3 21 266 85 94 92 203
Cuts at: 3817 1186
Size: 4516
Fragments arranged by size:
4516 1867 266 203 94 92 85 21
3
BseYI C'CCAG_C
Cuts at: 2500 2816 2934 3530 3778 3932 6350 6846 2500
Size: 316 118 596 248 154 2418 496 2801
Fragments arranged by size:
2801 2418 596 496 316 248 154 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2611 3834 3935 1163
Size: 573 875 1223 101 4375
Fragments arranged by size:
4375 1223 875 573 101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4597 5358 6386 1540
Size: 275 186 2596 761 1028 2301
Fragments arranged by size:
2596 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2348 3179 3341 4313 5208 5293 6364 2348
Size: 831 162 972 895 85 1071 3131
Fragments arranged by size:
3131 1071 972 895 831 162 85
BsmI GAATG_Cn'
Cuts at: 2848 4658 4751 2848
Size: 1810 93 5244
Fragments arranged by size:
5244 1810 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2348 3179 3341 3351 3606 3683 3829
Size: 399 558 831 162 10 255 77 146
Cuts at: 3829 3898 4059 4120 4197 4313 4396 4466 5208
Size: 69 161 61 77 116 83 70 742
Cuts at: 5208 5293 6364 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 831 742 558 399 255 162
161 146 116 85 83 77 77 70
69 61 10
BspEI T'CCGG_A
Cuts at: 6810 6810
Size: 7147
BspHI T'CATG_A
Cuts at: 2077 2544 5036 6766 2077
Size: 467 2492 1730 2458
Fragments arranged by size:
2492 2458 1730 467
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2499 3217 4502 844
Size: 1655 718 1285 3489
Fragments arranged by size:
3489 1655 1285 718
BsrBI CCG'CTC
Cuts at: 1394 1823 3613 3831 4398 5034 1394
Size: 429 1790 218 567 636 3507
Fragments arranged by size:
3507 1790 636 567 429 218
BsrDI GCAATG_nn'
Cuts at: 65 1248 2951 5642 5816 65
Size: 1183 1703 2691 174 1396
Fragments arranged by size:
2691 1703 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4281 5791 5978 1700
Size: 110 2471 1510 187 2869
Fragments arranged by size:
2869 2471 1510 187 110
BsrGI T'GTAC_A
Cuts at: 96 2900 96
Size: 2804 4343
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7147
BssSI C'ACGA_G
Cuts at: 5207 6219 5207
Size: 1012 6135
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7147
BstBI TT'CG_AA
Cuts at: 5951 5951
Size: 7147
BstEII G'GTnAC_C
Cuts at: 1184 3284 1184
Size: 2100 5047
BstXI CCAn_nnnn'nTGG
Cuts at: 2788 3778 4103 4444 2788
Size: 990 325 341 5491
Fragments arranged by size:
5491 990 341 325
BstYI r'GATC_y
Cuts at: 1972 2889 3565 3667 4003 4477 4844 5229 5246
Size: 917 676 102 336 474 367 385 17
Cuts at: 5246 6698 6784 6796 7142 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 917 676 474 385 367 346
336 102 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3684 4198 3684
Size: 514 6633
BtgI C'CryG_G
Cuts at: 513 1065 2004 3075 3476 513
Size: 552 939 1071 401 4184
Fragments arranged by size:
4184 1071 939 552 401
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6408
Fragments arranged by size:
6408 683 56
BtsI GCAGTG_nn'
Cuts at: 2221 4663 5457 5477 2221
Size: 2442 794 20 3891
Fragments arranged by size:
3891 2442 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7147
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7112
DraI TTT'AAA
Cuts at: 4451 4451
Size: 7147
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2374 6154 809
Size: 507 1058 3780 1802
Fragments arranged by size:
3780 1802 1058 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2743 2988
Size: 54 1425 50 275 18 220 693 245
Cuts at: 2988 4111 4594 5483 7112 8
Size: 1123 483 889 1629 43
Fragments arranged by size:
1629 1425 1123 889 693 483 275 245
220 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4594 1537
Size: 275 2782 4090
Fragments arranged by size:
4090 2782 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2137 2955 3220 4318 5077 1353
Size: 784 818 265 1098 759 3423
Fragments arranged by size:
3423 1098 818 784 759 265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5734 6108 6254 387
Size: 1064 138 4145 374 146 1280
Fragments arranged by size:
4145 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2346 4311 2346
Size: 1965 5182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2564 2904 2960 3327 3376 3608 3635
Size: 393 932 340 56 367 49 232 27
Cuts at: 3635 3730 3984 4049 5210 5807 6594 6862 1239
Size: 95 254 65 1161 597 787 268 1524
Fragments arranged by size:
1524 1161 932 787 597 393 367 340
268 254 232 95 65 56 49 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3191 3770 3191
Size: 579 6568
EcoO109I rG'GnC_Cy
Cuts at: 2394 2461 3275 3347 3348 3377 3680 4117 2394
Size: 67 814 72 1 29 303 437 5424
Fragments arranged by size:
5424 814 437 303 72 67 29 1
EcoRI G'AATT_C
Cuts at: 4456 4834 4456
Size: 378 6769
EcoRV GAT'ATC
Cuts at: 3423 3423
Size: 7147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3993 3993
Size: 7147
FspI TGC'GCA
Cuts at: 5653 5653
Size: 7147
HaeII r_GCGC'y
Cuts at: 1678 2310 6294 1678
Size: 632 3984 2531
Fragments arranged by size:
3984 2531 632
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2239 2271 2466 2498 3407
Size: 32 135 32 667 32 195 32 909
Cuts at: 3407 3439 3501 3533 3602 3634 4229 4261 5788
Size: 32 62 32 69 32 595 32 1527
Cuts at: 5788 5820 5862 5894 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1527 909 667 595 195 135 69
62 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2797 4491 4737 669
Size: 988 281 859 1694 246 3079
Fragments arranged by size:
3079 1694 988 859 281 246
HindIII A'AGCT_T
Cuts at: 748 3016 748
Size: 2268 4879
HpaI GTT'AAC
Cuts at: 4737 4737
Size: 7147
KpnI G_GTAC'C
Cuts at: 4026 4472 4026
Size: 446 6701
MfeI C'AATT_G
Cuts at: 4746 4746
Size: 7147
MluI A'CGCG_T
Cuts at: 7023 7023
Size: 7147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6261 6445 1305
Size: 4956 184 2007
Fragments arranged by size:
4956 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2052 2745 2990 10
Size: 54 1425 343 220 693 245 4167
Fragments arranged by size:
4167 1425 693 343 245 220 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2649 2786 4442 5105 5464 5623 518
Size: 648 1483 137 1656 663 359 159 2042
Fragments arranged by size:
2042 1656 1483 663 648 359 159 137
MspA1I CmG'CkG
Cuts at: 1606 2178 2938 3170 3266 3823 4180 4275 4563
Size: 572 760 232 96 557 357 95 288
Cuts at: 4563 5240 6388 6633 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 760 677 572 557 357 288
245 232 96 95
NcoI C'CATG_G
Cuts at: 513 1065 2004 3075 3476 513
Size: 552 939 1071 401 4184
Fragments arranged by size:
4184 1071 939 552 401
NdeI CA'TA_TG
Cuts at: 387 3358 387
Size: 2971 4176
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7147
NotI GC'GGCC_GC
Cuts at: 4594 4594
Size: 7147
NspI r_CATG'y
Cuts at: 1132 1229 3914 4505 6050 1132
Size: 97 2685 591 1545 2229
Fragments arranged by size:
2685 2229 1545 591 97
PasI CC'CwG_GG
Cuts at: 1348 3404 1348
Size: 2056 5091
PciI A'CATG_T
Cuts at: 3910 6046 3910
Size: 2136 5011
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2059 3259 1108
Size: 951 1200 4996
Fragments arranged by size:
4996 1200 951
PfoI T'CCnGG_A
Cuts at: 2463 3980 2463
Size: 1517 5630
PmeI GTTT'AAAC
Cuts at: 4451 4451
Size: 7147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5216 5248 6729 6761 5216
Size: 32 1481 32 5602
Fragments arranged by size:
5602 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2394 2461 2394
Size: 67 7080
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7147
PsiI TTA'TAA
Cuts at: 4717 4717
Size: 7147
PspOMI G'GGCC_C
Cuts at: 3347 3679 3825 4116 4392 3347
Size: 332 146 291 276 6102
Fragments arranged by size:
6102 332 291 276 146
PstI C_TGCA'G
Cuts at: 830 2228 2496 3123 4499 830
Size: 1398 268 627 1376 3478
Fragments arranged by size:
3478 1398 1376 627 268
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7147
PvuII CAG'CTG
Cuts at: 2178 2938 3170 3266 4180 2178
Size: 760 232 96 914 5145
Fragments arranged by size:
5145 914 760 232 96
SacI G_AGCT'C
Cuts at: 2348 4313 2348
Size: 1965 5182
SalI G'TCGA_C
Cuts at: 1936 4489 1936
Size: 2553 4594
SanDI GG'GwC_CC
Cuts at: 2461 2461
Size: 7147
SapI GCTCTTCn'nnn_
Cuts at: 4318 4318
Size: 7147
SbfI CC_TGCA'GG
Cuts at: 3123 4499 3123
Size: 1376 5771
ScaI AGT'ACT
Cuts at: 5395 7107 5395
Size: 1712 5435
SexAI A'CCwGG_T
Cuts at: 2862 4018 2862
Size: 1156 5991
SfcI C'TryA_G
Cuts at: 826 1045 2224 2492 3119 4495 5630 6311 6502
Size: 219 1179 268 627 1376 1135 681 191
Cuts at: 6502 826
Size: 1471
Fragments arranged by size:
1471 1376 1179 1135 681 627 268 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2742 2742
Size: 7147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7147
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7147
SmaI CCC'GGG
Cuts at: 1872 3326 4474 1872
Size: 1454 1148 4545
Fragments arranged by size:
4545 1454 1148
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3371 4008 5251 6152 6414 820
Size: 197 926 1428 637 1243 901 262 1553
Fragments arranged by size:
1553 1428 1243 926 901 637 262 197
SnaBI TAC'GTA
Cuts at: 493 4938 493
Size: 4445 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7147
SphI G_CATG'C
Cuts at: 1132 4505 1132
Size: 3373 3774
SspI AAT'ATT
Cuts at: 5 52 2996 5071 5
Size: 47 2944 2075 2081
Fragments arranged by size:
2944 2081 2075 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7147
StyI C'CwwG_G
Cuts at: 513 1065 2004 3075 3476 3771 4586 513
Size: 552 939 1071 401 295 815 3074
Fragments arranged by size:
3074 1071 939 815 552 401 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2542 2657 3238 5174 5191 5344 5529
Size: 1255 962 115 581 1936 17 153 185
Cuts at: 5529 5974 325
Size: 445 1498
Fragments arranged by size:
1936 1498 1255 962 581 445 185 153
115 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2806 2900 5393
Size: 274 80 33 51 1443 829 94 2493
Cuts at: 5393 7105 96
Size: 1712 138
Fragments arranged by size:
2493 1712 1443 829 274 138 94 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3320 3352 3721 3753 4373 4405 7107 7139 3320
Size: 32 369 32 620 32 2702 32 3328
Fragments arranged by size:
3328 2702 620 369 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3515 1316
Size: 2199 4948
XbaI T'CTAG_A
Cuts at: 4483 4483
Size: 7147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3029 3029
Size: 7147
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7147
XmaI C'CCGG_G
Cuts at: 1870 3324 4472 1870
Size: 1454 1148 4545
Fragments arranged by size:
4545 1454 1148
XmnI GAAnn'nnTTC
Cuts at: 3973 5276 3973
Size: 1303 5844
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3232 4955 276
Size: 53 83 186 936 1698 1723 2468
Fragments arranged by size:
2468 1723 1698 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I DraIII FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SfoI SrfI SwaI