Restriction map for
pFN21AE9431
(Circular) (Six-base) MAPSORT of: pFN21AE9431.seq Check: 7202 from: 1 to: 6532
pFN21AE9431 6532 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2599 2599
Size: 6532
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4342 278
Size: 53 83 186 936 2806 2468
Fragments arranged by size:
2806 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3875 1937
Size: 1938 4594
Acc65I G'GTAC_C
Cuts at: 3853 3853
Size: 6532
AclI AA'CG_TT
Cuts at: 1563 4659 5032 1563
Size: 3096 373 3063
Fragments arranged by size:
3096 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2785 4595 5979 6247 1632
Size: 1153 1810 1384 268 1917
Fragments arranged by size:
1917 1810 1384 1153 268
AfeI AGC'GCT
Cuts at: 2468 3029 2468
Size: 561 5971
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6335
AflIII A'CryG_T
Cuts at: 1203 3207 5431 6408 1203
Size: 2004 2224 977 1327
Fragments arranged by size:
2224 2004 1327 977
AgeI A'CCGG_T
Cuts at: 5363 5363
Size: 6532
AhdI GACnn_n'nnGTC
Cuts at: 2971 5261 2971
Size: 2290 4242
AleI CACnn'nnGTG
Cuts at: 1166 2654 1166
Size: 1488 5044
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6532
AlwNI CAG_nnn'CTG
Cuts at: 2599 2875 3547 4275 5847 2599
Size: 276 672 728 1572 3284
Fragments arranged by size:
3284 1572 728 672 276
ApaI G_GGCC'C
Cuts at: 3303 3471 3567 3303
Size: 168 96 6268
Fragments arranged by size:
6268 168 96
ApaLI G'TGCA_C
Cuts at: 2387 4589 5745 2387
Size: 2202 1156 3174
Fragments arranged by size:
3174 2202 1156
ApoI r'AATT_y
Cuts at: 1493 3841 4067 4219 6328 1493
Size: 2348 226 152 2109 1697
Fragments arranged by size:
2348 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5086 160
Size: 872 4054 1606
Fragments arranged by size:
4054 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
AvaI C'yCGr_G
Cuts at: 1870 1943 3302 3464 3560 3719 3787 3857 1870
Size: 73 1359 162 96 159 68 70 4545
Fragments arranged by size:
4545 1359 162 159 96 73 70 68
BamHI G'GATC_C
Cuts at: 3862 4229 3862
Size: 367 6165
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2616 3309 3853 5308
Size: 325 217 627 315 514 693 544 1455
Cuts at: 5308 618
Size: 1842
Fragments arranged by size:
1842 1455 693 627 544 514 325 315
217
BanII G_rGCy'C
Cuts at: 1391 2709 3254 3303 3430 3471 3567 3647 3720
Size: 1318 545 49 127 41 96 80 73
Cuts at: 3720 3824 3851 1391
Size: 104 27 4072
Fragments arranged by size:
4072 1318 545 127 104 96 80 73
49 41 27
BbeI G_GCGC'C
Cuts at: 2106 2620 3313 2106
Size: 514 693 5325
Fragments arranged by size:
5325 693 514
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2251 2746 928
Size: 971 352 495 4714
Fragments arranged by size:
4714 971 495 352
BbvCI CC'TCA_GC
Cuts at: 2093 2824 2093
Size: 731 5801
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2242 2276 2563 2597 4723 4757 1903
Size: 34 305 34 287 34 2126 34 3678
Fragments arranged by size:
3678 2126 305 287 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4424 5634 4424
Size: 1210 5322
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6532
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2775 2957 3181
Size: 107 122 71 631 605 1103 182 224
Cuts at: 3181 3275 3298 3307 3724 5143 136
Size: 94 23 9 417 1419 1525
Fragments arranged by size:
1525 1419 1103 631 605 417 224 182
122 107 94 71 23 9
BglII A'GATC_T
Cuts at: 3090 6527 3090
Size: 3437 3095
BlpI GC'TnA_GC
Cuts at: 2363 3949 2363
Size: 1586 4946
Bme1580I G_kGCm'C
Cuts at: 1790 2184 2391 2649 3303 3471 3567 4593 5749
Size: 394 207 258 654 168 96 1026 1156
Cuts at: 5749 1790
Size: 2573
Fragments arranged by size:
2573 1156 1026 654 394 258 207 168
96
BmgBI CAC'GTC
Cuts at: 1318 3208 1318
Size: 1890 4642
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4263 5221 442
Size: 416 53 83 399 24 2846 958 1753
Fragments arranged by size:
2846 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3122 5192 1239
Size: 1883 2070 2579
Fragments arranged by size:
2579 2070 1883
Bpu10I CC'TnA_GC
Cuts at: 2093 2824 3698 2093
Size: 731 874 4927
Fragments arranged by size:
4927 874 731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4657 5522 5820 4657
Size: 865 298 5369
Fragments arranged by size:
5369 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2252 2978 5195 882
Size: 617 753 726 2217 2219
Fragments arranged by size:
2219 2217 753 726 617
BsaAI yAC'GTr
Cuts at: 493 1204 4323 493
Size: 711 3119 2702
Fragments arranged by size:
3119 2702 711
BsaBI GATnn'nnATC
Cuts at: 6526 6526
Size: 6532
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 2523 2617 2820
Size: 53 83 186 936 570 420 94 203
Cuts at: 2820 3310 3507 3620 4339 4721 5356 275
Size: 490 197 113 719 382 635 1451
Fragments arranged by size:
1451 936 719 635 570 490 420 382
203 197 186 113 94 83 53
BsaWI w'CCGG_w
Cuts at: 4965 5363 5637 5784 6195 4965
Size: 398 274 147 411 5302
Fragments arranged by size:
5302 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6307
Fragments arranged by size:
6307 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2677 2984 1186
Size: 1491 307 4734
Fragments arranged by size:
4734 1491 307
BseYI C'CCAG_C
Cuts at: 3117 3246 3636 3817 5735 6231 3117
Size: 129 390 181 1918 496 3418
Fragments arranged by size:
3418 1918 496 390 181 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2055 2225 2557 2627 3788 1163
Size: 573 319 170 332 70 1161 3907
Fragments arranged by size:
3907 1161 573 332 319 170 70
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2908 3375 3982 4743 5771 1540
Size: 275 186 907 467 607 761 1028 2301
Fragments arranged by size:
2301 1028 907 761 607 467 275 186
BsiHKAI G_wGCw'C
Cuts at: 2391 2861 4593 4678 5749 2391
Size: 470 1732 85 1071 3174
Fragments arranged by size:
3174 1732 1071 470 85
BsmI GAATG_Cn'
Cuts at: 4043 4136 4043
Size: 93 6439
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2391 2649 2709 2861 3254 3303
Size: 399 394 207 258 60 152 393 49
Cuts at: 3303 3430 3471 3567 3647 3720 3824 3851 4593
Size: 127 41 96 80 73 104 27 742
Cuts at: 4593 4678 5749 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 393 258 207
152 127 104 96 85 80 73 60
49 41 27
BspEI T'CCGG_A
Cuts at: 6195 6195
Size: 6532
BspHI T'CATG_A
Cuts at: 4421 6151 4421
Size: 1730 4802
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2599 3157 3171 3887 844
Size: 1755 558 14 716 3489
Fragments arranged by size:
3489 1755 716 558 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 3307 3369 3480 4419 1394
Size: 429 194 1290 62 111 939 3507
Fragments arranged by size:
3507 1290 939 429 194 111 62
BsrDI GCAATG_nn'
Cuts at: 65 1248 2374 5027 5201 65
Size: 1183 1126 2653 174 1396
Fragments arranged by size:
2653 1396 1183 1126 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2358 3677 5176 5363 1700
Size: 110 548 1319 1499 187 2869
Fragments arranged by size:
2869 1499 1319 548 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6532
BssHII G'CGCG_C
Cuts at: 1925 3357 3359 3730 1925
Size: 1432 2 371 4727
Fragments arranged by size:
4727 1432 371 2
BssSI C'ACGA_G
Cuts at: 2738 4592 5604 2738
Size: 1854 1012 3666
Fragments arranged by size:
3666 1854 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2386 3163 3286 794
Size: 1592 777 123 4040
Fragments arranged by size:
4040 1592 777 123
BstBI TT'CG_AA
Cuts at: 5336 5336
Size: 6532
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6532
BstXI CCAn_nnnn'nTGG
Cuts at: 2656 2659 3325 2656
Size: 3 666 5863
Fragments arranged by size:
5863 666 3
BstYI r'GATC_y
Cuts at: 1972 3090 3601 3862 4229 4614 4631 6083 6169
Size: 1118 511 261 367 385 17 1452 86
Cuts at: 6169 6181 6527 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1118 511 385 367 346 261
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2199 2479 2559 2730 2862 3419 513
Size: 552 1134 280 80 171 132 557 3626
Fragments arranged by size:
3626 1134 557 552 280 171 132 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2170 2566 487
Size: 683 56 944 396 4453
Fragments arranged by size:
4453 944 683 396 56
BtsI GCAGTG_nn'
Cuts at: 2038 2043 3128 4048 4842 4862 2038
Size: 5 1085 920 794 20 3708
Fragments arranged by size:
3708 1085 920 794 20 5
ClaI AT'CG_AT
Cuts at: 1554 2694 1554
Size: 1140 5392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6497
DraI TTT'AAA
Cuts at: 3836 3836
Size: 6532
DraIII CAC_nnn'GTG
Cuts at: 3286 3286
Size: 6532
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5539 809
Size: 507 4223 1802
Fragments arranged by size:
4223 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2154 2175
Size: 54 1425 50 275 18 243 81 21
Cuts at: 2175 2289 2556 2601 2958 3078 3267 3372 3979
Size: 114 267 45 357 120 189 105 607
Cuts at: 3979 4868 6497 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 607 357 275 267 243
189 120 114 105 81 54 50 45
43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3372 3979 1537
Size: 275 1560 607 4090
Fragments arranged by size:
4090 1560 607 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2594 4462 1353
Size: 1241 1868 3423
Fragments arranged by size:
3423 1868 1241
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3085 3472 5119 5493 5639 387
Size: 1064 138 1496 387 1647 374 146 1280
Fragments arranged by size:
1647 1496 1280 1064 387 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2785 3122 4595 5192 5979 6247 1239
Size: 393 1153 337 1473 597 787 268 1524
Fragments arranged by size:
1524 1473 1153 787 597 393 337 268
EcoO109I rG'GnC_Cy
Cuts at: 2059 2872 3109 3169 3262 3544 3564 3613 2059
Size: 813 237 60 93 282 20 49 4978
Fragments arranged by size:
4978 813 282 237 93 60 49 20
EcoRI G'AATT_C
Cuts at: 3841 4219 3841
Size: 378 6154
FspI TGC'GCA
Cuts at: 3666 5038 3666
Size: 1372 5160
HaeII r_GCGC'y
Cuts at: 1678 2106 2224 2470 2620 2686 2831 3031 3187
Size: 428 118 246 150 66 145 200 156
Cuts at: 3187 3313 3636 5679 1678
Size: 126 323 2043 2531
Fragments arranged by size:
2531 2043 428 323 246 200 156 150
145 126 118 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2957 2989 5173 5205 5247
Size: 32 135 32 1385 32 2184 32 42
Cuts at: 5247 5279 1373
Size: 32 2626
Fragments arranged by size:
2626 2184 1385 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2353 3876 4122 669
Size: 988 281 415 1523 246 3079
Fragments arranged by size:
3079 1523 988 415 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6532
HpaI GTT'AAC
Cuts at: 4122 4122
Size: 6532
KasI G'GCGC_C
Cuts at: 2102 2616 3309 2102
Size: 514 693 5325
Fragments arranged by size:
5325 693 514
KpnI G_GTAC'C
Cuts at: 3857 3857
Size: 6532
MfeI C'AATT_G
Cuts at: 4131 4131
Size: 6532
MluI A'CGCG_T
Cuts at: 6408 6408
Size: 6532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3003 5646 5830 1305
Size: 1698 2643 184 2007
Fragments arranged by size:
2643 2007 1698 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 2291 2558 2960 3080
Size: 54 1425 343 243 216 267 402 120
Cuts at: 3080 10
Size: 3462
Fragments arranged by size:
3462 1425 402 343 267 243 216 120
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2333 2396 2564 2654 2657 3198
Size: 648 996 171 63 168 90 3 541
Cuts at: 3198 4490 4849 5008 518
Size: 1292 359 159 2042
Fragments arranged by size:
2042 1292 996 648 541 359 171 168
159 90 63 3
MspA1I CmG'CkG
Cuts at: 1606 2209 3226 3421 3575 3640 3948 4625 5773
Size: 603 1017 195 154 65 308 677 1148
Cuts at: 5773 6018 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1017 677 603 308 245 195
154 65
NaeI GCC'GGC
Cuts at: 3679 3679
Size: 6532
NarI GG'CG_CC
Cuts at: 2103 2617 3310 2103
Size: 514 693 5325
Fragments arranged by size:
5325 693 514
NcoI C'CATG_G
Cuts at: 513 1065 2479 2559 513
Size: 552 1414 80 4486
Fragments arranged by size:
4486 1414 552 80
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6532
NgoMIV G'CCGG_C
Cuts at: 3677 3677
Size: 6532
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6532
NotI GC'GGCC_GC
Cuts at: 3979 3979
Size: 6532
NspI r_CATG'y
Cuts at: 1132 1229 2167 2332 3890 5435 1132
Size: 97 938 165 1558 1545 2229
Fragments arranged by size:
2229 1558 1545 938 165 97
PasI CC'CwG_GG
Cuts at: 1348 3647 1348
Size: 2299 4233
PciI A'CATG_T
Cuts at: 5431 5431
Size: 6532
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2082 2599 1108
Size: 974 517 5041
Fragments arranged by size:
5041 974 517
PfoI T'CCnGG_A
Cuts at: 2934 3604 2934
Size: 670 5862
PmeI GTTT'AAAC
Cuts at: 3836 3836
Size: 6532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4601 4633 6114 6146 4601
Size: 32 1481 32 4987
Fragments arranged by size:
4987 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3169 3262 3169
Size: 93 6439
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6532
PsiI TTA'TAA
Cuts at: 4102 4102
Size: 6532
PspOMI G'GGCC_C
Cuts at: 3299 3467 3563 3299
Size: 168 96 6268
Fragments arranged by size:
6268 168 96
PstI C_TGCA'G
Cuts at: 830 2727 3168 3884 830
Size: 1897 441 716 3478
Fragments arranged by size:
3478 1897 716 441
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6532
PvuII CAG'CTG
Cuts at: 2209 2209
Size: 6532
SacII CC_GC'GG
Cuts at: 3422 3422
Size: 6532
SalI G'TCGA_C
Cuts at: 1936 3874 1936
Size: 1938 4594
SbfI CC_TGCA'GG
Cuts at: 3168 3884 3168
Size: 716 5816
ScaI AGT'ACT
Cuts at: 4780 6492 4780
Size: 1712 4820
SexAI A'CCwGG_T
Cuts at: 3015 3282 3015
Size: 267 6265
SfcI C'TryA_G
Cuts at: 826 1045 2723 3164 3880 5015 5696 5887 826
Size: 219 1678 441 716 1135 681 191 1471
Fragments arranged by size:
1678 1471 1135 716 681 441 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2957 2957
Size: 6532
SfoI GGC'GCC
Cuts at: 2104 2618 3311 2104
Size: 514 693 5325
Fragments arranged by size:
5325 693 514
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6532
SmaI CCC'GGG
Cuts at: 1872 3466 3562 3721 3859 1872
Size: 1594 96 159 138 4545
Fragments arranged by size:
4545 1594 159 138 96
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4636 5537 5799 820
Size: 197 926 2693 901 262 1553
Fragments arranged by size:
2693 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4323 493
Size: 3830 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6532
SphI G_CATG'C
Cuts at: 1132 3890 1132
Size: 2758 3774
SrfI GCCC'GGGC
Cuts at: 3562 3562
Size: 6532
SspI AAT'ATT
Cuts at: 5 52 4456 5
Size: 47 4404 2081
Fragments arranged by size:
4404 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6532
StyI C'CwwG_G
Cuts at: 513 1065 2127 2479 2559 3971 513
Size: 552 1062 352 80 1412 3074
Fragments arranged by size:
3074 1412 1062 552 352 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2174 2492 4559 4576 4729 4914 5359
Size: 1255 594 318 2067 17 153 185 445
Cuts at: 5359 325
Size: 1498
Fragments arranged by size:
2067 1498 1255 594 445 318 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4778 6490 96
Size: 274 80 33 51 1443 2801 1712 138
Fragments arranged by size:
2801 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6492 6524 6492
Size: 32 6500
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6532
XbaI T'CTAG_A
Cuts at: 3868 3868
Size: 6532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2197 2656 2197
Size: 459 6073
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6532
XmaI C'CCGG_G
Cuts at: 1870 3464 3560 3719 3857 1870
Size: 1594 96 159 138 4545
Fragments arranged by size:
4545 1594 159 138 96
XmnI GAAnn'nnTTC
Cuts at: 4661 4661
Size: 6532
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4340 276
Size: 53 83 186 936 2806 2468
Fragments arranged by size:
2806 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SanDI SapI SwaI