Restriction map for
pFN21AE9427
(Circular) (Six-base) MAPSORT of: pFN21AE9427.seq Check: 5368 from: 1 to: 7327
pFN21AE9427 7327 bp
With 182 enzymes: SgfI *
March 28, 2012 11:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3649 3649
Size: 7327
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5137 278
Size: 53 83 186 936 3601 2468
Fragments arranged by size:
3601 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2082 2550 4611 4670 1937
Size: 145 468 2061 59 4594
Fragments arranged by size:
4594 2061 468 145 59
Acc65I G'GTAC_C
Cuts at: 4313 4648 4313
Size: 335 6992
AclI AA'CG_TT
Cuts at: 1563 5454 5827 1563
Size: 3891 373 3063
Fragments arranged by size:
3891 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 2331 2467 3532 5390 6774 7042 1632
Size: 527 172 136 1065 1858 1384 268 1917
Fragments arranged by size:
1917 1858 1384 1065 527 268 172 136
AfeI AGC'GCT
Cuts at: 3806 3806
Size: 7327
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7130
AflIII A'CryG_T
Cuts at: 1203 3479 4371 6226 7203 1203
Size: 2276 892 1855 977 1327
Fragments arranged by size:
2276 1855 1327 977 892
AgeI A'CCGG_T
Cuts at: 6158 6158
Size: 7327
AhdI GACnn_n'nnGTC
Cuts at: 6056 6056
Size: 7327
AleI CACnn'nnGTG
Cuts at: 1166 3272 3582 1166
Size: 2106 310 4911
Fragments arranged by size:
4911 2106 310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7327
AlwNI CAG_nnn'CTG
Cuts at: 2280 3433 3856 4402 5070 6642 2280
Size: 1153 423 546 668 1572 2965
Fragments arranged by size:
2965 1572 1153 668 546 423
ApaLI G'TGCA_C
Cuts at: 5384 6540 5384
Size: 1156 6171
ApoI r'AATT_y
Cuts at: 1493 3184 4432 4636 4862 5014 7123 1493
Size: 1691 1248 204 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 1248 226 204 152
AseI AT'TA_AT
Cuts at: 160 1032 5881 160
Size: 872 4849 1606
Fragments arranged by size:
4849 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7327
AvaI C'yCGr_G
Cuts at: 1870 1943 3878 4652 1870
Size: 73 1935 774 4545
Fragments arranged by size:
4545 1935 774 73
BamHI G'GATC_C
Cuts at: 2163 4657 5024 2163
Size: 2494 367 4466
Fragments arranged by size:
4466 2494 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4136 4313 4648 6103 618
Size: 325 217 627 2349 177 335 1455 1842
Fragments arranged by size:
2349 1842 1455 627 335 325 217 177
BanII G_rGCy'C
Cuts at: 1391 2707 3134 3443 4646 1391
Size: 1316 427 309 1203 4072
Fragments arranged by size:
4072 1316 1203 427 309
BbeI G_GCGC'C
Cuts at: 4140 4140
Size: 7327
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2345 2760 3514 928
Size: 971 446 415 754 4741
Fragments arranged by size:
4741 971 754 446 415
BbvCI CC'TCA_GC
Cuts at: 2918 2918
Size: 7327
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4180 4214 5518 5552 1903
Size: 34 2243 34 1304 34 3678
Fragments arranged by size:
3678 2243 1304 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2173 2923 3097 5219 6429 2173
Size: 750 174 2122 1210 3071
Fragments arranged by size:
3071 2122 1210 750 174
BclI T'GATC_A
Cuts at: 1548 3987 1548
Size: 2439 4888
BfrBI ATG'CAT
Cuts at: 3352 3352
Size: 7327
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2493 4145 5938
Size: 107 122 71 631 605 821 1652 1793
Cuts at: 5938 136
Size: 1525
Fragments arranged by size:
1793 1652 1525 821 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7322 7322
Size: 7327
BlpI GC'TnA_GC
Cuts at: 4571 4744 4571
Size: 173 7154
Bme1580I G_kGCm'C
Cuts at: 1790 3890 5388 6544 1790
Size: 2100 1498 1156 2573
Fragments arranged by size:
2573 2100 1498 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7327
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2922 4254 5058
Size: 416 53 83 399 24 1505 1332 804
Cuts at: 5058 6016 442
Size: 958 1753
Fragments arranged by size:
1753 1505 1332 958 804 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7327
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3151 3620 3151
Size: 469 6858
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2071 2168 2208 2401 3283 3419 3692 5987
Size: 832 97 40 193 882 136 273 2295
Cuts at: 5987 1239
Size: 2579
Fragments arranged by size:
2579 2295 882 832 273 193 136 97
40
Bpu10I CC'TnA_GC
Cuts at: 2567 2918 2567
Size: 351 6976
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3449 5452 6317 6615 3449
Size: 2003 865 298 4161
Fragments arranged by size:
4161 2003 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4248 5990 882
Size: 617 2749 1742 2219
Fragments arranged by size:
2749 2219 1742 617
BsaAI yAC'GTr
Cuts at: 493 1204 3482 5118 493
Size: 711 2278 1636 2702
Fragments arranged by size:
2702 2278 1636 711
BsaBI GATnn'nnATC
Cuts at: 4064 7321 4064
Size: 3257 4070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 2123 4137 5134
Size: 53 83 186 936 546 44 2014 997
Cuts at: 5134 5516 6151 275
Size: 382 635 1451
Fragments arranged by size:
2014 1451 997 936 635 546 382 186
83 53 44
BsaWI w'CCGG_w
Cuts at: 2713 3554 3795 5760 6158 6432 6579 6990 2713
Size: 841 241 1965 398 274 147 411 3050
Fragments arranged by size:
3050 1965 841 411 398 274 241 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2181 2211 2261 2291 3536
Size: 30 165 30 778 30 50 30 1245
Cuts at: 3536 3549 3566 3579 1178
Size: 13 17 13 4926
Fragments arranged by size:
4926 1245 778 165 50 30 30 30
30 17 13 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3124 1186
Size: 1938 5389
BseYI C'CCAG_C
Cuts at: 6530 7026 6530
Size: 496 6831
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3083 3610 3953 1163
Size: 573 1347 527 343 4537
Fragments arranged by size:
4537 1347 573 527 343
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4777 5538 6566 1540
Size: 275 186 2776 761 1028 2301
Fragments arranged by size:
2776 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2573 3269 3741 3791 3918 5388 5473 6544 2573
Size: 696 472 50 127 1470 85 1071 3356
Fragments arranged by size:
3356 1470 1071 696 472 127 85 50
BsmI GAATG_Cn'
Cuts at: 4838 4931 4838
Size: 93 7234
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2573 2707 3134 3269 3443 3741 3791
Size: 399 783 134 427 135 174 298 50
Cuts at: 3791 3890 3918 4646 5388 5473 6544 1391
Size: 99 28 728 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 728 427 399 298
174 135 134 99 85 50 28
BspEI T'CCGG_A
Cuts at: 2713 3554 3795 6990 2713
Size: 841 241 3195 3050
Fragments arranged by size:
3195 3050 841 241
BspHI T'CATG_A
Cuts at: 2790 4533 5216 6946 2790
Size: 1743 683 1730 3171
Fragments arranged by size:
3171 1743 1730 683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2256 3105 3649 4682 844
Size: 1412 849 544 1033 3489
Fragments arranged by size:
3489 1412 1033 849 544
BsrBI CCG'CTC
Cuts at: 1394 1823 2701 5214 1394
Size: 429 878 2513 3507
Fragments arranged by size:
3507 2513 878 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5822 5996 65
Size: 1183 4574 174 1396
Fragments arranged by size:
4574 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5971 6158 1700
Size: 110 4161 187 2869
Fragments arranged by size:
4161 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2445 96
Size: 2349 4978
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7327
BssSI C'ACGA_G
Cuts at: 2598 2627 3239 3592 4318 4483 5387 6399 2598
Size: 29 612 353 726 165 904 1012 3526
Fragments arranged by size:
3526 1012 904 726 612 353 165 29
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4113 794
Size: 3319 4008
BstBI TT'CG_AA
Cuts at: 6131 6131
Size: 7327
BstEII G'GTnAC_C
Cuts at: 1184 3118 3760 1184
Size: 1934 642 4751
Fragments arranged by size:
4751 1934 642
BstXI CCAn_nnnn'nTGG
Cuts at: 4288 4288
Size: 7327
BstYI r'GATC_y
Cuts at: 1972 2163 2994 3231 4245 4657 5024 5409 5426
Size: 191 831 237 1014 412 367 385 17
Cuts at: 5426 6878 6964 6976 7322 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1014 831 412 385 367 346
237 191 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2985 3039 3123 3141 3156 3211
Size: 552 939 981 54 84 18 15 55
Cuts at: 3211 3577 4086 4249 4623 513
Size: 366 509 163 374 3217
Fragments arranged by size:
3217 981 939 552 509 374 366 163
84 55 54 18 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4183 487
Size: 683 56 2957 3631
Fragments arranged by size:
3631 2957 683 56
BtsI GCAGTG_nn'
Cuts at: 2306 2736 4293 4843 5637 5657 2306
Size: 430 1557 550 794 20 3976
Fragments arranged by size:
3976 1557 794 550 430 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7327
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7292
DraI TTT'AAA
Cuts at: 4631 4631
Size: 7327
DraIII CAC_nnn'GTG
Cuts at: 3694 3694
Size: 7327
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6334 809
Size: 507 5018 1802
Fragments arranged by size:
5018 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2141 3159 3750
Size: 54 1425 50 275 18 311 1018 591
Cuts at: 3750 3909 4290 4774 5663 7292 8
Size: 159 381 484 889 1629 43
Fragments arranged by size:
1629 1425 1018 889 591 484 381 311
275 159 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4774 1537
Size: 275 2962 4090
Fragments arranged by size:
4090 2962 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2792 2853 4059 5257 1353
Size: 1439 61 1206 1198 3423
Fragments arranged by size:
3423 1439 1206 1198 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3863 4483 5914 6288 6434 387
Size: 1064 138 2274 620 1431 374 146 1280
Fragments arranged by size:
2274 1431 1280 1064 620 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2159 2168 2208 2331 2401 2467
Size: 393 439 88 9 40 123 70 66
Cuts at: 2467 3283 3419 3532 3692 5390 5987 6774 7042
Size: 816 136 113 160 1698 597 787 268
Cuts at: 7042 1239
Size: 1524
Fragments arranged by size:
1698 1524 816 787 597 439 393 268
160 136 123 113 88 70 66 40
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2231 3250 3656 2231
Size: 1019 406 5902
Fragments arranged by size:
5902 1019 406
EcoO109I rG'GnC_Cy
Cuts at: 2495 2539 2562 3853 4542 2495
Size: 44 23 1291 689 5280
Fragments arranged by size:
5280 1291 689 44 23
EcoRI G'AATT_C
Cuts at: 3184 4432 4636 5014 3184
Size: 1248 204 378 5497
Fragments arranged by size:
5497 1248 378 204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 7327
FspI TGC'GCA
Cuts at: 5833 5833
Size: 7327
HaeII r_GCGC'y
Cuts at: 1678 3039 3701 3808 4140 4233 6474 1678
Size: 1361 662 107 332 93 2241 2531
Fragments arranged by size:
2531 2241 1361 662 332 107 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2376 2408 3536 3549 3568
Size: 32 135 32 804 32 1128 13 19
Cuts at: 3568 3581 5968 6000 6042 6074 1373
Size: 13 2387 32 42 32 2626
Fragments arranged by size:
2626 2387 1128 804 135 42 32 32
32 32 32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2212 2557 2623 4183 4275 4345
Size: 988 281 274 345 66 1560 92 70
Cuts at: 4345 4612 4671 4917 669
Size: 267 59 246 3079
Fragments arranged by size:
3079 1560 988 345 281 274 267 246
92 70 66 59
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7327
HpaI GTT'AAC
Cuts at: 4917 4917
Size: 7327
KasI G'GCGC_C
Cuts at: 4136 4136
Size: 7327
KpnI G_GTAC'C
Cuts at: 4317 4652 4317
Size: 335 6992
MfeI C'AATT_G
Cuts at: 4111 4926 4111
Size: 815 6512
MluI A'CGCG_T
Cuts at: 7203 7203
Size: 7327
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2640 2993 3117 3984 4530 6441 6625 1305
Size: 1335 353 124 867 546 1911 184 2007
Fragments arranged by size:
2007 1911 1335 867 546 353 184 124
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2143 3161 3752 10
Size: 54 1425 343 311 1018 591 3585
Fragments arranged by size:
3585 1425 1018 591 343 311 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3074 3272 3582 3653 3659 4202 5285
Size: 648 1908 198 310 71 6 543 1083
Cuts at: 5285 5644 5803 518
Size: 359 159 2042
Fragments arranged by size:
2042 1908 1083 648 543 359 310 198
159 71 6
MspA1I CmG'CkG
Cuts at: 1606 2421 3624 3670 4743 5420 6568 6813 1606
Size: 815 1203 46 1073 677 1148 245 2120
Fragments arranged by size:
2120 1203 1148 1073 815 677 245 46
NarI GG'CG_CC
Cuts at: 4137 4137
Size: 7327
NcoI C'CATG_G
Cuts at: 513 1065 2004 3141 4086 4249 513
Size: 552 939 1137 945 163 3591
Fragments arranged by size:
3591 1137 945 939 552 163
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7327
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7327
NotI GC'GGCC_GC
Cuts at: 4774 4774
Size: 7327
NsiI A_TGCA'T
Cuts at: 3354 3354
Size: 7327
NspI r_CATG'y
Cuts at: 1132 1229 2759 3298 4375 4685 6230 1132
Size: 97 1530 539 1077 310 1545 2229
Fragments arranged by size:
2229 1545 1530 1077 539 310 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7327
PciI A'CATG_T
Cuts at: 4371 6226 4371
Size: 1855 5472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2469 3907 4473 1108
Size: 1361 1438 566 3962
Fragments arranged by size:
3962 1438 1361 566
PfoI T'CCnGG_A
Cuts at: 2104 2881 3540 2104
Size: 777 659 5891
Fragments arranged by size:
5891 777 659
PmeI GTTT'AAAC
Cuts at: 4631 4631
Size: 7327
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5396 5428 6909 6941 5396
Size: 32 1481 32 5782
Fragments arranged by size:
5782 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2562 3853 4542 2562
Size: 1291 689 5347
Fragments arranged by size:
5347 1291 689
PshAI GACnn'nnGTC
Cuts at: 1613 2510 4211 1613
Size: 897 1701 4729
Fragments arranged by size:
4729 1701 897
PsiI TTA'TAA
Cuts at: 4897 4897
Size: 7327
PspXI vC'TCGA_Gb
Cuts at: 3878 3878
Size: 7327
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 7295
PstI C_TGCA'G
Cuts at: 830 4398 4679 830
Size: 3568 281 3478
Fragments arranged by size:
3568 3478 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7327
SalI G'TCGA_C
Cuts at: 1936 4610 4669 1936
Size: 2674 59 4594
Fragments arranged by size:
4594 2674 59
SanDI GG'GwC_CC
Cuts at: 2562 4542 2562
Size: 1980 5347
SbfI CC_TGCA'GG
Cuts at: 4398 4679 4398
Size: 281 7046
ScaI AGT'ACT
Cuts at: 5575 7287 5575
Size: 1712 5615
SexAI A'CCwGG_T
Cuts at: 3690 3690
Size: 7327
SfcI C'TryA_G
Cuts at: 826 1045 3976 4394 4675 5810 6491 6682 826
Size: 219 2931 418 281 1135 681 191 1471
Fragments arranged by size:
2931 1471 1135 681 418 281 219 191
SfoI GGC'GCC
Cuts at: 4138 4138
Size: 7327
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7327
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7327
SmaI CCC'GGG
Cuts at: 1872 4654 1872
Size: 2782 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3464 3878 5431 6332 6594 820
Size: 197 926 1521 414 1553 901 262 1553
Fragments arranged by size:
1553 1553 1521 926 901 414 262 197
SnaBI TAC'GTA
Cuts at: 493 5118 493
Size: 4625 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7327
SphI G_CATG'C
Cuts at: 1132 2759 3298 4685 1132
Size: 1627 539 1387 3774
Fragments arranged by size:
3774 1627 1387 539
SspI AAT'ATT
Cuts at: 5 52 5251 5
Size: 47 5199 2081
Fragments arranged by size:
5199 2081 47
StuI AGG'CCT
Cuts at: 1517 2477 3245 1517
Size: 960 768 5599
Fragments arranged by size:
5599 960 768
StyI C'CwwG_G
Cuts at: 513 1065 2004 2532 3141 4086 4249 4281 4467
Size: 552 939 528 609 945 163 32 186
Cuts at: 4467 4577 4766 513
Size: 110 189 3074
Fragments arranged by size:
3074 945 939 609 552 528 189 186
163 110 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3430 5354 5371 5524 5709 6154 325
Size: 1255 1850 1924 17 153 185 445 1498
Fragments arranged by size:
1924 1850 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2445 2730 3528
Size: 274 80 33 51 1443 468 285 798
Cuts at: 3528 4368 5573 7285 96
Size: 840 1205 1712 138
Fragments arranged by size:
1712 1443 1205 840 798 468 285 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2100 2132 2181 2213 2563 2595 3377 3409 3678
Size: 32 49 32 350 32 782 32 269
Cuts at: 3678 3710 7287 7319 2100
Size: 32 3577 32 2108
Fragments arranged by size:
3577 2108 782 350 269 49 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3071 1316
Size: 1755 5572
XbaI T'CTAG_A
Cuts at: 2357 4663 2357
Size: 2306 5021
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2499 2499
Size: 7327
XhoI C'TCGA_G
Cuts at: 1943 3878 1943
Size: 1935 5392
XmaI C'CCGG_G
Cuts at: 1870 4652 1870
Size: 2782 4545
XmnI GAAnn'nnTTC
Cuts at: 4517 5456 4517
Size: 939 6388
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5135 276
Size: 53 83 186 936 3601 2468
Fragments arranged by size:
3601 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BsiWI BsmBI BstZ17I
Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI
PacI PmlI PspOMI PvuII RsrII SacI SacII SapI
SfiI SrfI SwaI