Restriction map for
pFN21AE9426
(Circular) (Six-base) MAPSORT of: pFN21AE9426.seq Check: 8889 from: 1 to: 7096
pFN21AE9426 7096 bp
With 182 enzymes: SgfI *
March 28, 2012 11:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4906 278
Size: 53 83 186 936 3370 2468
Fragments arranged by size:
3370 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4439 1937
Size: 2502 4594
Acc65I G'GTAC_C
Cuts at: 4417 4417
Size: 7096
AclI AA'CG_TT
Cuts at: 1563 3321 5223 5596 1563
Size: 1758 1902 373 3063
Fragments arranged by size:
3063 1902 1758 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2511 2778 2909 4090 4233 5159 6543 6811
Size: 879 267 131 1181 143 926 1384 268
Cuts at: 6811 1632
Size: 1917
Fragments arranged by size:
1917 1384 1181 926 879 268 267 143
131
AfeI AGC'GCT
Cuts at: 3646 3646
Size: 7096
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6899
AflIII A'CryG_T
Cuts at: 1203 2315 5995 6972 1203
Size: 1112 3680 977 1327
Fragments arranged by size:
3680 1327 1112 977
AgeI A'CCGG_T
Cuts at: 5927 5927
Size: 7096
AhdI GACnn_n'nnGTC
Cuts at: 5825 5825
Size: 7096
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7096
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7096
AlwNI CAG_nnn'CTG
Cuts at: 3976 4839 6411 3976
Size: 863 1572 4661
Fragments arranged by size:
4661 1572 863
ApaLI G'TGCA_C
Cuts at: 3330 3580 5153 6309 3330
Size: 250 1573 1156 4117
Fragments arranged by size:
4117 1573 1156 250
ApoI r'AATT_y
Cuts at: 1493 2785 3128 3163 4149 4405 4631 4783 6892
Size: 1292 343 35 986 256 226 152 2109
Cuts at: 6892 1493
Size: 1697
Fragments arranged by size:
2109 1697 1292 986 343 256 226 152
35
AseI AT'TA_AT
Cuts at: 160 1032 3266 3719 5650 160
Size: 872 2234 453 1931 1606
Fragments arranged by size:
2234 1931 1606 872 453
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7096
AvaI C'yCGr_G
Cuts at: 1870 1943 4421 1870
Size: 73 2478 4545
Fragments arranged by size:
4545 2478 73
BamHI G'GATC_C
Cuts at: 2109 4426 4793 2109
Size: 2317 367 4412
Fragments arranged by size:
4412 2317 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4417 5872 618
Size: 325 217 627 2630 1455 1842
Fragments arranged by size:
2630 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4415 1391
Size: 3024 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4096 4174 928
Size: 971 2197 78 3850
Fragments arranged by size:
3850 2197 971 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3934 3968 4081 4115 5287 5321 1903
Size: 34 1997 34 113 34 1172 34 3678
Fragments arranged by size:
3678 1997 1172 113 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2320 4988 6198 2320
Size: 2668 1210 3218
Fragments arranged by size:
3218 2668 1210
BclI T'GATC_A
Cuts at: 1548 2103 1548
Size: 555 6541
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5707 136
Size: 107 122 71 631 605 4035 1525
Fragments arranged by size:
4035 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7091 7091
Size: 7096
BlpI GC'TnA_GC
Cuts at: 4513 4513
Size: 7096
Bme1580I G_kGCm'C
Cuts at: 1790 3215 3334 3584 5157 6313 1790
Size: 1425 119 250 1573 1156 2573
Fragments arranged by size:
2573 1573 1425 1156 250 119
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7096
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2093 2279 3190
Size: 416 53 83 399 24 676 186 911
Cuts at: 3190 4827 5785 442
Size: 1637 958 1753
Fragments arranged by size:
1753 1637 958 911 676 416 399 186
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2850 1056
Size: 1794 5302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2113 5756 1239
Size: 874 3643 2579
Fragments arranged by size:
3643 2579 874
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3034 5221 6086 6384 3034
Size: 2187 865 298 3746
Fragments arranged by size:
3746 2187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5759 882
Size: 617 4260 2219
Fragments arranged by size:
4260 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4887 493
Size: 711 3683 2702
Fragments arranged by size:
3683 2702 711
BsaBI GATnn'nnATC
Cuts at: 2102 2108 7090 2102
Size: 6 4982 2108
Fragments arranged by size:
4982 2108 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4903 5285 5920 275
Size: 53 83 186 936 3370 382 635 1451
Fragments arranged by size:
3370 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2358 5529 5927 6201 6348 6759 2358
Size: 3171 398 274 147 411 2695
Fragments arranged by size:
3171 2695 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6871
Fragments arranged by size:
6871 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3443 1186
Size: 2257 4839
BseYI C'CCAG_C
Cuts at: 3736 6299 6795 3736
Size: 2563 496 4037
Fragments arranged by size:
4037 2563 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2386 2855 3587 3633 1163
Size: 573 650 469 732 46 4626
Fragments arranged by size:
4626 732 650 573 469 46
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4262 4546 5307 6335 1540
Size: 275 186 2261 284 761 1028 2301
Fragments arranged by size:
2301 2261 1028 761 284 275 186
BsiHKAI G_wGCw'C
Cuts at: 3334 3584 5157 5242 6313 3334
Size: 250 1573 85 1071 4117
Fragments arranged by size:
4117 1573 1071 250 85
BsmI GAATG_Cn'
Cuts at: 4607 4700 4607
Size: 93 7003
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3215 3334 3584 4415 5157 5242 6313
Size: 399 1425 119 250 831 742 85 1071
Cuts at: 6313 1391
Size: 2174
Fragments arranged by size:
2174 1425 1071 831 742 399 250 119
85
BspEI T'CCGG_A
Cuts at: 6759 6759
Size: 7096
BspHI T'CATG_A
Cuts at: 4985 6715 4985
Size: 1730 5366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2440 4451 844
Size: 1596 2011 3489
Fragments arranged by size:
3489 2011 1596
BsrBI CCG'CTC
Cuts at: 1394 1823 4983 1394
Size: 429 3160 3507
Fragments arranged by size:
3507 3160 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2428 3925 4241 4298 5591 5765 65
Size: 1183 1180 1497 316 57 1293 174 1396
Fragments arranged by size:
1497 1396 1293 1183 1180 316 174 57
BsrFI r'CCGG_y
Cuts at: 1700 1810 3813 5740 5927 1700
Size: 110 2003 1927 187 2869
Fragments arranged by size:
2869 2003 1927 187 110
BsrGI T'GTAC_A
Cuts at: 96 2198 2225 4161 96
Size: 2102 27 1936 3031
Fragments arranged by size:
3031 2102 1936 27
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7096
BssSI C'ACGA_G
Cuts at: 5156 6168 5156
Size: 1012 6084
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7096
BstBI TT'CG_AA
Cuts at: 5900 5900
Size: 7096
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7096
BstYI r'GATC_y
Cuts at: 1972 2109 4426 4793 5178 5195 6647 6733 6745
Size: 137 2317 367 385 17 1452 86 12
Cuts at: 6745 7091 1972
Size: 346 1977
Fragments arranged by size:
2317 1977 1452 385 367 346 137 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2030 2598 4292 2030
Size: 568 1694 4834
Fragments arranged by size:
4834 1694 568
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5605
Fragments arranged by size:
5605 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6357
Fragments arranged by size:
6357 683 56
BtsI GCAGTG_nn'
Cuts at: 3582 4612 5406 5426 3582
Size: 1030 794 20 5252
Fragments arranged by size:
5252 1030 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7096
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7061
DraI TTT'AAA
Cuts at: 4400 4400
Size: 7096
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6103 809
Size: 507 4787 1802
Fragments arranged by size:
4787 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4259 4543 5432
Size: 54 1425 50 275 18 2429 284 889
Cuts at: 5432 7061 8
Size: 1629 43
Fragments arranged by size:
2429 1629 1425 889 284 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4259 4543 1537
Size: 275 2447 284 4090
Fragments arranged by size:
4090 2447 284 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 2797 3693 5026 1353
Size: 846 598 896 1333 3423
Fragments arranged by size:
3423 1333 896 846 598
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5683 6057 6203 387
Size: 1064 138 4094 374 146 1280
Fragments arranged by size:
4094 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2113 2511 2778 2909 4090 4233 5159
Size: 393 481 398 267 131 1181 143 926
Cuts at: 5159 5756 6543 6811 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1181 926 787 597 481 398 393
268 267 143 131
EcoO109I rG'GnC_Cy
Cuts at: 2810 2810
Size: 7096
EcoRI G'AATT_C
Cuts at: 4405 4783 4405
Size: 378 6718
FspI TGC'GCA
Cuts at: 5602 5602
Size: 7096
HaeII r_GCGC'y
Cuts at: 1678 3648 6243 1678
Size: 1970 2595 2531
Fragments arranged by size:
2595 2531 1970
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2073 2105 3299 3331 3717
Size: 32 135 32 501 32 1194 32 386
Cuts at: 3717 3749 5737 5769 5811 5843 1373
Size: 32 1988 32 42 32 2626
Fragments arranged by size:
2626 1988 1194 501 386 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2633 3094 4440 4686 669
Size: 988 281 695 461 1346 246 3079
Fragments arranged by size:
3079 1346 988 695 461 281 246
HindIII A'AGCT_T
Cuts at: 748 2993 4194 748
Size: 2245 1201 3650
Fragments arranged by size:
3650 2245 1201
HpaI GTT'AAC
Cuts at: 4686 4686
Size: 7096
KpnI G_GTAC'C
Cuts at: 4421 4421
Size: 7096
MfeI C'AATT_G
Cuts at: 2229 4695 2229
Size: 2466 4630
MluI A'CGCG_T
Cuts at: 6972 6972
Size: 7096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2542 3304 3342 6210 6394 1305
Size: 1237 762 38 2868 184 2007
Fragments arranged by size:
2868 2007 1237 762 184 38
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5274
Fragments arranged by size:
5274 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2886 3434 5054 5413 5572 518
Size: 648 1720 548 1620 359 159 2042
Fragments arranged by size:
2042 1720 1620 648 548 359 159
MspA1I CmG'CkG
Cuts at: 1606 3237 3874 4512 5189 6337 6582 1606
Size: 1631 637 638 677 1148 245 2120
Fragments arranged by size:
2120 1631 1148 677 638 637 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5605
Fragments arranged by size:
5605 939 552
NdeI CA'TA_TG
Cuts at: 387 3190 387
Size: 2803 4293
NheI G'CTAG_C
Cuts at: 1052 2846 1052
Size: 1794 5302
NotI GC'GGCC_GC
Cuts at: 4543 4543
Size: 7096
NspI r_CATG'y
Cuts at: 1132 1229 2319 2967 4454 5999 1132
Size: 97 1090 648 1487 1545 2229
Fragments arranged by size:
2229 1545 1487 1090 648 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7096
PciI A'CATG_T
Cuts at: 2315 5995 2315
Size: 3680 3416
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7096
PmeI GTTT'AAAC
Cuts at: 4400 4400
Size: 7096
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5165 5197 6678 6710 5165
Size: 32 1481 32 5551
Fragments arranged by size:
5551 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7096
PsiI TTA'TAA
Cuts at: 3863 4666 3863
Size: 803 6293
PstI C_TGCA'G
Cuts at: 830 2162 4448 830
Size: 1332 2286 3478
Fragments arranged by size:
3478 2286 1332
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7096
SalI G'TCGA_C
Cuts at: 1936 4438 1936
Size: 2502 4594
SbfI CC_TGCA'GG
Cuts at: 4448 4448
Size: 7096
ScaI AGT'ACT
Cuts at: 4348 5344 7056 4348
Size: 996 1712 4388
Fragments arranged by size:
4388 1712 996
SfcI C'TryA_G
Cuts at: 826 1045 2148 2158 3741 4444 5579 6260 6451
Size: 219 1103 10 1583 703 1135 681 191
Cuts at: 6451 826
Size: 1471
Fragments arranged by size:
1583 1471 1135 1103 703 681 219 191
10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7096
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7096
SmaI CCC'GGG
Cuts at: 1872 4423 1872
Size: 2551 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3049 5200 6101 6363 820
Size: 197 926 1106 2151 901 262 1553
Fragments arranged by size:
2151 1553 1106 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4887 493
Size: 4394 2702
SpeI A'CTAG_T
Cuts at: 152 2543 152
Size: 2391 4705
SphI G_CATG'C
Cuts at: 1132 4454 1132
Size: 3322 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5020 5
Size: 47 3468 1500 2081
Fragments arranged by size:
3468 2081 1500 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7096
StyI C'CwwG_G
Cuts at: 513 1065 2004 4535 513
Size: 552 939 2531 3074
Fragments arranged by size:
3074 2531 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5123 5140 5293 5478 5923 325
Size: 1255 3543 17 153 185 445 1498
Fragments arranged by size:
3543 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2198 2225 3114
Size: 274 80 33 51 1443 221 27 889
Cuts at: 3114 4161 4346 5342 7054 96
Size: 1047 185 996 1712 138
Fragments arranged by size:
1712 1443 1047 996 889 274 221 185
138 80 51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7056 7088 7056
Size: 32 7064
Tth111I GACn'n_nGTC
Cuts at: 1316 3098 1316
Size: 1782 5314
XbaI T'CTAG_A
Cuts at: 4432 4432
Size: 7096
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2284
Size: 7096
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7096
XmaI C'CCGG_G
Cuts at: 1870 4421 1870
Size: 2551 4545
XmnI GAAnn'nnTTC
Cuts at: 2590 5225 2590
Size: 2635 4461
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4904 276
Size: 53 83 186 936 3370 2468
Fragments arranged by size:
3370 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI