Restriction map for
pFN21AE9424
(Circular) (Six-base) MAPSORT of: pFN21AE9424.seq Check: 1620 from: 1 to: 5788
pFN21AE9424 5788 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2349 2439 2349
Size: 90 5698
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2097 3598 278
Size: 53 83 186 936 561 1501 2468
Fragments arranged by size:
2468 1501 936 561 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 1937
Size: 1194 4594
Acc65I G'GTAC_C
Cuts at: 3109 3109
Size: 5788
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3851 5235 5503 1632
Size: 2219 1384 268 1917
Fragments arranged by size:
2219 1917 1384 268
AfeI AGC'GCT
Cuts at: 2275 2275
Size: 5788
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 2178 4687 5664 1203
Size: 975 2509 977 1327
Fragments arranged by size:
2509 1327 977 975
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 3531 5103 3531
Size: 1572 4216
ApaI G_GGCC'C
Cuts at: 2327 2327
Size: 5788
ApaLI G'TGCA_C
Cuts at: 2426 3845 5001 2426
Size: 1419 1156 3213
Fragments arranged by size:
3213 1419 1156
ApoI r'AATT_y
Cuts at: 1493 2574 2691 3000 3097 3323 3475 5584 1493
Size: 1081 117 309 97 226 152 2109 1697
Fragments arranged by size:
2109 1697 1081 309 226 152 117 97
AscI GG'CGCG_CC
Cuts at: 2201 2201
Size: 5788
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 3113 1870
Size: 73 1170 4545
Fragments arranged by size:
4545 1170 73
BamHI G'GATC_C
Cuts at: 3118 3485 3118
Size: 367 5421
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2561 2656 3109 4564 618
Size: 325 217 627 774 95 453 1455 1842
Fragments arranged by size:
1842 1455 774 627 453 325 217 95
BanII G_rGCy'C
Cuts at: 1391 2327 2840 3107 1391
Size: 936 513 267 4072
Fragments arranged by size:
4072 936 513 267
BbeI G_GCGC'C
Cuts at: 2565 2565
Size: 5788
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2521 928
Size: 971 622 4195
Fragments arranged by size:
4195 971 622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 2676 1548
Size: 1128 4660
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4399 136
Size: 107 122 71 631 605 2727 1525
Fragments arranged by size:
2727 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2616 5783 2616
Size: 3167 2621
BlpI GC'TnA_GC
Cuts at: 3205 3205
Size: 5788
Bme1580I G_kGCm'C
Cuts at: 1790 2327 2430 3849 5005 1790
Size: 537 103 1419 1156 2573
Fragments arranged by size:
2573 1419 1156 537 103
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3519 4477 442
Size: 416 53 83 399 24 2102 958 1753
Fragments arranged by size:
2102 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2426 2631 4448 1239
Size: 836 351 205 1817 2579
Fragments arranged by size:
2579 1817 836 351 205
Bpu10I CC'TnA_GC
Cuts at: 2363 2363
Size: 5788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3913 4778 5076 3913
Size: 865 298 4625
Fragments arranged by size:
4625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2821 4451 882
Size: 617 1322 1630 2219
Fragments arranged by size:
2219 1630 1322 617
BsaAI yAC'GTr
Cuts at: 493 1204 2353 3579 493
Size: 711 1149 1226 2702
Fragments arranged by size:
2702 1226 1149 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 2212 2562 3595
Size: 53 83 186 936 561 118 350 1033
Cuts at: 3595 3977 4612 275
Size: 382 635 1451
Fragments arranged by size:
1451 1033 936 635 561 382 350 186
118 83 53
BsaWI w'CCGG_w
Cuts at: 4221 4619 4893 5040 5451 4221
Size: 398 274 147 411 4558
Fragments arranged by size:
4558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5563
Fragments arranged by size:
5563 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2798 2849 1186
Size: 1612 51 4125
Fragments arranged by size:
4125 1612 51
BseYI C'CCAG_C
Cuts at: 2170 2320 2772 4991 5487 2170
Size: 150 452 2219 496 2471
Fragments arranged by size:
2471 2219 496 452 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3001 1163
Size: 573 1265 3950
Fragments arranged by size:
3950 1265 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2508 3238 3999 5027 1540
Size: 275 186 507 730 761 1028 2301
Fragments arranged by size:
2301 1028 761 730 507 275 186
BsiHKAI G_wGCw'C
Cuts at: 2151 2430 3849 3934 5005 2151
Size: 279 1419 85 1071 2934
Fragments arranged by size:
2934 1419 1071 279 85
BsmI GAATG_Cn'
Cuts at: 2021 3299 3392 2021
Size: 1278 93 4417
Fragments arranged by size:
4417 1278 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2327 2430 2840 3107 3849 3934
Size: 399 361 176 103 410 267 742 85
Cuts at: 3934 5005 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 410 399 361 267 176
103 85
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2268 2349 2439 3143 844
Size: 1424 81 90 704 3489
Fragments arranged by size:
3489 1424 704 90 81
BsrBI CCG'CTC
Cuts at: 1394 1823 2367 2513 3675 1394
Size: 429 544 146 1162 3507
Fragments arranged by size:
3507 1162 544 429 146
BsrDI GCAATG_nn'
Cuts at: 65 1248 4283 4457 65
Size: 1183 3035 174 1396
Fragments arranged by size:
3035 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2109 4432 4619 1700
Size: 110 299 2323 187 2869
Fragments arranged by size:
2869 2323 299 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 2019 2201 1925
Size: 94 182 5512
Fragments arranged by size:
5512 182 94
BssSI C'ACGA_G
Cuts at: 2620 3848 4860 2620
Size: 1228 1012 3548
Fragments arranged by size:
3548 1228 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2181 2418 794
Size: 1387 237 4164
Fragments arranged by size:
4164 1387 237
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 2616 2760 3118 3485 3870 3887 5339 5425
Size: 644 144 358 367 385 17 1452 86
Cuts at: 5425 5437 5783 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 644 385 367 358 346 144
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2124 2215 2394 513
Size: 552 1059 91 179 3907
Fragments arranged by size:
3907 1059 552 179 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 2166 2478 3304 4098 4118 2166
Size: 312 826 794 20 3836
Fragments arranged by size:
3836 826 794 312 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3053 4795 809
Size: 507 1737 1742 1802
Fragments arranged by size:
1802 1742 1737 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2292 2391
Size: 54 1425 50 275 18 291 171 99
Cuts at: 2391 2900 3235 4124 5753 8
Size: 509 335 889 1629 43
Fragments arranged by size:
1629 1425 889 509 335 291 275 171
99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2252 2270 2351 2714 2795 2807 2915 3718
Size: 899 18 81 363 81 12 108 803
Cuts at: 3718 1353
Size: 3423
Fragments arranged by size:
3423 899 803 363 108 81 81 18
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4375 4749 4895 387
Size: 1064 138 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2426 2631 3851 4448 5235 5503
Size: 393 443 351 205 1220 597 787 268
Cuts at: 5503 1239
Size: 1524
Fragments arranged by size:
1524 1220 787 597 443 393 351 268
205
EcoO109I rG'GnC_Cy
Cuts at: 2324 2450 2324
Size: 126 5662
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FspI TGC'GCA
Cuts at: 2048 4294 2048
Size: 2246 3542
HaeII r_GCGC'y
Cuts at: 1678 2277 2486 2565 4935 1678
Size: 599 209 79 2370 2531
Fragments arranged by size:
2531 2370 599 209 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4429 4461 4503 4535 1373
Size: 32 135 32 2857 32 42 32 2626
Fragments arranged by size:
2857 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KasI G'GCGC_C
Cuts at: 2561 2561
Size: 5788
KpnI G_GTAC'C
Cuts at: 3113 3113
Size: 5788
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2115 4902 5086 1305
Size: 810 2787 184 2007
Fragments arranged by size:
2787 2007 810 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3966
Fragments arranged by size:
3966 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3746 4105 4264 518
Size: 648 2580 359 159 2042
Fragments arranged by size:
2580 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2126 2217 2396 3204 3881 5029 5274
Size: 424 96 91 179 808 677 1148 245
Cuts at: 5274 1606
Size: 2120
Fragments arranged by size:
2120 1148 808 677 424 245 179 96
91
NarI GG'CG_CC
Cuts at: 2562 2562
Size: 5788
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5236
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 3146 4691 1132
Size: 97 1917 1545 2229
Fragments arranged by size:
2229 1917 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5788
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2113 1108
Size: 1005 4783
PfoI T'CCnGG_A
Cuts at: 2608 2608
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2450 2450
Size: 5788
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PspOMI G'GGCC_C
Cuts at: 2323 2323
Size: 5788
PstI C_TGCA'G
Cuts at: 830 2436 3140 830
Size: 1606 704 3478
Fragments arranged by size:
3478 1606 704
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
SacII CC_GC'GG
Cuts at: 2127 2218 2397 2127
Size: 91 179 5518
Fragments arranged by size:
5518 179 91
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SapI GCTCTTCn'nnn_
Cuts at: 2270 2351 2795 2270
Size: 81 444 5263
Fragments arranged by size:
5263 444 81
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SexAI A'CCwGG_T
Cuts at: 2889 2889
Size: 5788
SfcI C'TryA_G
Cuts at: 826 1045 2432 3136 4271 4952 5143 826
Size: 219 1387 704 1135 681 191 1471
Fragments arranged by size:
1471 1387 1135 704 681 219 191
SfoI GGC'GCC
Cuts at: 2563 2563
Size: 5788
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 3115 1872
Size: 1243 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3892 4793 5055 820
Size: 197 926 1949 901 262 1553
Fragments arranged by size:
1949 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 2312 1517
Size: 795 4993
StyI C'CwwG_G
Cuts at: 513 1065 3227 513
Size: 552 2162 3074
Fragments arranged by size:
3074 2162 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2908 3815 3832 3985 4170 4615 325
Size: 1255 1328 907 17 153 185 445 1498
Fragments arranged by size:
1498 1328 1255 907 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4034 5746 96
Size: 274 80 33 51 1443 2057 1712 138
Fragments arranged by size:
2057 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2062 2094 5748 5780 2062
Size: 32 3654 32 2070
Fragments arranged by size:
3654 2070 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2891 1316
Size: 1575 4213
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2386 2386
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 3113 1870
Size: 1243 4545
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2095 3596 276
Size: 53 83 186 936 561 1501 2468
Fragments arranged by size:
2468 1501 936 561 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacI SanDI SfiI SrfI
SwaI