Restriction map for
pFN21AE9423
(Circular) (Six-base) MAPSORT of: pFN21AE9423.seq Check: 6445 from: 1 to: 7141
pFN21AE9423 7141 bp
With 182 enzymes: SgfI *
March 28, 2012 11:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2417 3009 4213 2417
Size: 592 1204 5345
Fragments arranged by size:
5345 1204 592
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4951 278
Size: 53 83 186 936 3415 2468
Fragments arranged by size:
3415 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3117 4484 1937
Size: 1180 1367 4594
Fragments arranged by size:
4594 1367 1180
Acc65I G'GTAC_C
Cuts at: 4462 4462
Size: 7141
AclI AA'CG_TT
Cuts at: 1563 4440 5268 5641 1563
Size: 2877 828 373 3063
Fragments arranged by size:
3063 2877 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3052 5204 6588 6856 1632
Size: 1420 2152 1384 268 1917
Fragments arranged by size:
2152 1917 1420 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6944
AflIII A'CryG_T
Cuts at: 1203 2349 2351 4131 6040 7017 1203
Size: 1146 2 1780 1909 977 1327
Fragments arranged by size:
1909 1780 1327 1146 977 2
AgeI A'CCGG_T
Cuts at: 5972 5972
Size: 7141
AhdI GACnn_n'nnGTC
Cuts at: 5870 5870
Size: 7141
AleI CACnn'nnGTG
Cuts at: 1166 2492 1166
Size: 1326 5815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3698 1184
Size: 2514 4627
AlwNI CAG_nnn'CTG
Cuts at: 2088 2446 2866 2926 4884 6456 2088
Size: 358 420 60 1958 1572 2773
Fragments arranged by size:
2773 1958 1572 420 358 60
ApaLI G'TGCA_C
Cuts at: 5198 6354 5198
Size: 1156 5985
ApoI r'AATT_y
Cuts at: 1493 2246 4066 4450 4676 4828 6937 1493
Size: 753 1820 384 226 152 2109 1697
Fragments arranged by size:
2109 1820 1697 753 384 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5695 160
Size: 872 4663 1606
Fragments arranged by size:
4663 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
AvaI C'yCGr_G
Cuts at: 1870 1943 2581 3397 4239 4466 1870
Size: 73 638 816 842 227 4545
Fragments arranged by size:
4545 842 816 638 227 73
BamHI G'GATC_C
Cuts at: 2473 3568 4471 4838 2473
Size: 1095 903 367 4776
Fragments arranged by size:
4776 1095 903 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2100 2405 3482 4462 5917
Size: 325 217 627 313 305 1077 980 1455
Cuts at: 5917 618
Size: 1842
Fragments arranged by size:
1842 1455 1077 980 627 325 313 305
217
BanII G_rGCy'C
Cuts at: 1391 4460 1391
Size: 3069 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3412 4279 928
Size: 971 1513 867 3790
Fragments arranged by size:
3790 1513 971 867
BbvCI CC'TCA_GC
Cuts at: 3216 3353 4313 3216
Size: 137 960 6044
Fragments arranged by size:
6044 960 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2208 2242 5332 5366 1903
Size: 34 271 34 3090 34 3678
Fragments arranged by size:
3678 3090 271 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5033 6243 5033
Size: 1210 5931
BclI T'GATC_A
Cuts at: 1548 3742 1548
Size: 2194 4947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2451 2583 2730
Size: 107 122 71 631 605 779 132 147
Cuts at: 2730 5752 136
Size: 3022 1525
Fragments arranged by size:
3022 1525 779 631 605 147 132 122
107 71
BglII A'GATC_T
Cuts at: 7136 7136
Size: 7141
BlpI GC'TnA_GC
Cuts at: 3602 4179 4558 3602
Size: 577 379 6185
Fragments arranged by size:
6185 577 379
Bme1580I G_kGCm'C
Cuts at: 1790 2095 2105 5202 6358 1790
Size: 305 10 3097 1156 2573
Fragments arranged by size:
3097 2573 1156 305 10
BmgBI CAC'GTC
Cuts at: 1318 4132 1318
Size: 2814 4327
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3356 3463 4872
Size: 416 53 83 399 24 1939 107 1409
Cuts at: 4872 5830 442
Size: 958 1753
Fragments arranged by size:
1939 1753 1409 958 416 399 107 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 3627 2671
Size: 956 6185
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2065 2838 2960 2978 3119 3848 3949 4381
Size: 826 773 122 18 141 729 101 432
Cuts at: 4381 5801 1239
Size: 1420 2579
Fragments arranged by size:
2579 1420 826 773 729 432 141 122
101 18
Bpu10I CC'TnA_GC
Cuts at: 3035 3216 3353 3521 4313 4407 3035
Size: 181 137 168 792 94 5769
Fragments arranged by size:
5769 792 181 168 137 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3154 3865 5266 6131 6429 3154
Size: 711 1401 865 298 3866
Fragments arranged by size:
3866 1401 865 711 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2954 5804 882
Size: 617 1455 2850 2219
Fragments arranged by size:
2850 2219 1455 617
BsaAI yAC'GTr
Cuts at: 493 1204 2352 4203 4932 493
Size: 711 1148 1851 729 2702
Fragments arranged by size:
2702 1851 1148 729 711
BsaBI GATnn'nnATC
Cuts at: 7135 7135
Size: 7141
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4948 5330 5965 275
Size: 53 83 186 936 3415 382 635 1451
Fragments arranged by size:
3415 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2510 3749 4143 5574 5972 6246 6393 6804 2510
Size: 1239 394 1431 398 274 147 411 2847
Fragments arranged by size:
2847 1431 1239 411 398 394 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2001 2031 3341 3371 3821
Size: 30 165 30 598 30 1310 30 450
Cuts at: 3821 3851 4097 4127 4379 4409 1178
Size: 30 246 30 252 30 3910
Fragments arranged by size:
3910 1310 598 450 252 246 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3578 3614 3713 4105 4301 1186
Size: 2392 36 99 392 196 4026
Fragments arranged by size:
4026 2392 392 196 99 36
BseYI C'CCAG_C
Cuts at: 3934 4427 6344 6840 3934
Size: 493 1917 496 4235
Fragments arranged by size:
4235 1917 496 493
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2311 2470 2671 3125 3578 3837 4183
Size: 573 575 159 201 454 453 259 346
Cuts at: 4183 1163
Size: 4121
Fragments arranged by size:
4121 575 573 454 453 346 259 201
159
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4591 5352 6380 1540
Size: 275 186 2590 761 1028 2301
Fragments arranged by size:
2590 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3414 3770 5202 5287 6358 3414
Size: 356 1432 85 1071 4197
Fragments arranged by size:
4197 1432 1071 356 85
BsmI GAATG_Cn'
Cuts at: 2036 2507 4652 4745 2036
Size: 471 2145 93 4432
Fragments arranged by size:
4432 2145 471 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3613 3613
Size: 7141
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2095 2105 3414 3770 4460 5202 5287
Size: 399 305 10 1309 356 690 742 85
Cuts at: 5287 6358 1391
Size: 1071 2174
Fragments arranged by size:
2174 1309 1071 742 690 399 356 305
85 10
BspEI T'CCGG_A
Cuts at: 2510 4143 6804 2510
Size: 1633 2661 2847
Fragments arranged by size:
2847 2661 1633
BspHI T'CATG_A
Cuts at: 3897 5030 6760 3897
Size: 1133 1730 4278
Fragments arranged by size:
4278 1730 1133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2417 2444 3009 4213 4312 4496 844
Size: 1573 27 565 1204 99 184 3489
Fragments arranged by size:
3489 1573 1204 565 184 99 27
BsrBI CCG'CTC
Cuts at: 1394 1823 5028 1394
Size: 429 3205 3507
Fragments arranged by size:
3507 3205 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5636 5810 65
Size: 1183 4388 174 1396
Fragments arranged by size:
4388 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3779 5785 5972 1700
Size: 110 1969 2006 187 2869
Fragments arranged by size:
2869 2006 1969 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7141
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7141
BssSI C'ACGA_G
Cuts at: 2116 3731 5201 6213 2116
Size: 1615 1470 1012 3044
Fragments arranged by size:
3044 1615 1470 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3504 794
Size: 2710 4431
BstBI TT'CG_AA
Cuts at: 5945 5945
Size: 7141
BstEII G'GTnAC_C
Cuts at: 1184 3188 1184
Size: 2004 5137
BstXI CCAn_nnnn'nTGG
Cuts at: 2188 2188
Size: 7141
BstYI r'GATC_y
Cuts at: 1972 2473 3568 4471 4838 5223 5240 6692 6778
Size: 501 1095 903 367 385 17 1452 86
Cuts at: 6778 6790 7136 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1095 903 501 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2106 2469 2106
Size: 363 6778
BtgI C'CryG_G
Cuts at: 513 1065 2004 2187 2700 513
Size: 552 939 183 513 4954
Fragments arranged by size:
4954 939 552 513 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3760 487
Size: 683 56 2534 3868
Fragments arranged by size:
3868 2534 683 56
BtsI GCAGTG_nn'
Cuts at: 2539 3156 3680 3849 4408 4657 5451 5471 2539
Size: 617 524 169 559 249 794 20 4209
Fragments arranged by size:
4209 794 617 559 524 249 169 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7141
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7106
DraI TTT'AAA
Cuts at: 4168 4445 4168
Size: 277 6864
DraIII CAC_nnn'GTG
Cuts at: 2989 3760 4206 2989
Size: 771 446 5924
Fragments arranged by size:
5924 771 446
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3125 6148 809
Size: 507 1809 3023 1802
Fragments arranged by size:
3023 1809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2892 2991 3433
Size: 54 1425 50 275 18 1062 99 442
Cuts at: 3433 3804 3948 4588 5477 7106 8
Size: 371 144 640 889 1629 43
Fragments arranged by size:
1629 1425 1062 889 640 442 371 275
144 99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4588 1537
Size: 275 2776 4090
Fragments arranged by size:
4090 2776 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3713 3731 3848 4262 5071 1353
Size: 2360 18 117 414 809 3423
Fragments arranged by size:
3423 2360 809 414 117 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5728 6102 6248 387
Size: 1064 138 4139 374 146 1280
Fragments arranged by size:
4139 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2838 2960 2978 3052 3119 3848
Size: 393 433 773 122 18 74 67 729
Cuts at: 3848 3949 4381 5204 5801 6588 6856 1239
Size: 101 432 823 597 787 268 1524
Fragments arranged by size:
1524 823 787 773 729 597 433 432
393 268 122 101 74 67 18
EcoO109I rG'GnC_Cy
Cuts at: 2604 2863 2944 2953 3220 3832 2604
Size: 259 81 9 267 612 5913
Fragments arranged by size:
5913 612 267 259 81 9
EcoRI G'AATT_C
Cuts at: 4450 4828 4450
Size: 378 6763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 7141
FspI TGC'GCA
Cuts at: 5647 5647
Size: 7141
HaeII r_GCGC'y
Cuts at: 1678 2927 6288 1678
Size: 1249 3361 2531
Fragments arranged by size:
3361 2531 1249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2001 2033 2736 2768 3127
Size: 32 135 32 429 32 703 32 359
Cuts at: 3127 3159 3302 3334 5782 5814 5856 5888 1373
Size: 32 143 32 2448 32 42 32 2626
Fragments arranged by size:
2626 2448 703 429 359 143 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2416 3874 4438 4485 4731 669
Size: 988 281 478 1458 564 47 246 3079
Fragments arranged by size:
3079 1458 988 564 478 281 246 47
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7141
HpaI GTT'AAC
Cuts at: 4438 4731 4438
Size: 293 6848
KpnI G_GTAC'C
Cuts at: 4466 4466
Size: 7141
MfeI C'AATT_G
Cuts at: 3738 4740 3738
Size: 1002 6139
MluI A'CGCG_T
Cuts at: 7017 7017
Size: 7141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4030 6255 6439 1305
Size: 2725 2225 184 2007
Fragments arranged by size:
2725 2225 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2894 2993 3435 3806 3950
Size: 54 1425 343 1062 99 442 371 144
Cuts at: 3950 10
Size: 3201
Fragments arranged by size:
3201 1425 1062 442 371 343 144 99
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2492 2670 3552 3593 3818 3978
Size: 648 1020 306 178 882 41 225 160
Cuts at: 3978 4154 5099 5458 5617 518
Size: 176 945 359 159 2042
Fragments arranged by size:
2042 1020 945 882 648 359 306 225
178 176 160 159 41
MspA1I CmG'CkG
Cuts at: 1606 2574 2715 2721 3938 3996 4183 4557 5234
Size: 968 141 6 1217 58 187 374 677
Cuts at: 5234 6382 6627 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1217 1148 968 677 374 245 187
141 58 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 2187 513
Size: 552 939 183 5467
Fragments arranged by size:
5467 939 552 183
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7141
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7141
NotI GC'GGCC_GC
Cuts at: 4588 4588
Size: 7141
NspI r_CATG'y
Cuts at: 1132 1229 4499 6044 1132
Size: 97 3270 1545 2229
Fragments arranged by size:
3270 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2948 3194 1348
Size: 1600 246 5295
Fragments arranged by size:
5295 1600 246
PciI A'CATG_T
Cuts at: 6040 6040
Size: 7141
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2866 1108
Size: 1758 5383
PfoI T'CCnGG_A
Cuts at: 2859 3231 4326 2859
Size: 372 1095 5674
Fragments arranged by size:
5674 1095 372
PmeI GTTT'AAAC
Cuts at: 4445 4445
Size: 7141
PmlI CAC'GTG
Cuts at: 2352 4203 2352
Size: 1851 5290
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5210 5242 6723 6755 5210
Size: 32 1481 32 5596
Fragments arranged by size:
5596 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2604 2863 2953 2604
Size: 259 90 6792
Fragments arranged by size:
6792 259 90
PshAI GACnn'nnGTC
Cuts at: 1613 2370 3869 1613
Size: 757 1499 4885
Fragments arranged by size:
4885 1499 757
PsiI TTA'TAA
Cuts at: 4711 4711
Size: 7141
PstI C_TGCA'G
Cuts at: 830 3082 3285 3473 3693 3961 4281 4493 830
Size: 2252 203 188 220 268 320 212 3478
Fragments arranged by size:
3478 2252 320 268 220 212 203 188
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7141
PvuII CAG'CTG
Cuts at: 2715 3938 4183 2715
Size: 1223 245 5673
Fragments arranged by size:
5673 1223 245
SalI G'TCGA_C
Cuts at: 1936 4483 1936
Size: 2547 4594
SanDI GG'GwC_CC
Cuts at: 2604 2604
Size: 7141
SbfI CC_TGCA'GG
Cuts at: 3082 4281 4493 3082
Size: 1199 212 5730
Fragments arranged by size:
5730 1199 212
ScaI AGT'ACT
Cuts at: 5389 7101 5389
Size: 1712 5429
SfcI C'TryA_G
Cuts at: 826 1045 3078 3281 3469 3689 3957 4277 4489
Size: 219 2033 203 188 220 268 320 212
Cuts at: 4489 5624 6305 6496 826
Size: 1135 681 191 1471
Fragments arranged by size:
2033 1471 1135 681 320 268 220 219
212 203 191 188
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7141
SmaI CCC'GGG
Cuts at: 1872 2583 4241 4468 1872
Size: 711 1658 227 4545
Fragments arranged by size:
4545 1658 711 227
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3133 3880 5245 6146 6408 820
Size: 197 926 1190 747 1365 901 262 1553
Fragments arranged by size:
1553 1365 1190 926 901 747 262 197
SnaBI TAC'GTA
Cuts at: 493 4932 493
Size: 4439 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7141
SphI G_CATG'C
Cuts at: 1132 4499 1132
Size: 3367 3774
SrfI GCCC'GGGC
Cuts at: 2583 2583
Size: 7141
SspI AAT'ATT
Cuts at: 5 52 5065 5
Size: 47 5013 2081
Fragments arranged by size:
5013 2081 47
StuI AGG'CCT
Cuts at: 1517 3033 3970 1517
Size: 1516 937 4688
Fragments arranged by size:
4688 1516 937
StyI C'CwwG_G
Cuts at: 513 1065 2004 2187 2384 2422 4036 4580 513
Size: 552 939 183 197 38 1614 544 3074
Fragments arranged by size:
3074 1614 939 552 544 197 183 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2552 3359 3530 5168 5185 5338 5523
Size: 1255 972 807 171 1638 17 153 185
Cuts at: 5523 5968 325
Size: 445 1498
Fragments arranged by size:
1638 1498 1255 972 807 445 185 171
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4302 5387 7099
Size: 274 80 33 51 1443 2325 1085 1712
Cuts at: 7099 96
Size: 138
Fragments arranged by size:
2325 1712 1443 1085 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2102 2134 3744 3776 4379 4411 7101 7133 2102
Size: 32 1610 32 603 32 2690 32 2110
Fragments arranged by size:
2690 2110 1610 603 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2774 2855 1316
Size: 1458 81 5602
Fragments arranged by size:
5602 1458 81
XbaI T'CTAG_A
Cuts at: 4477 4477
Size: 7141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2447 3001 3443 2447
Size: 554 442 6145
Fragments arranged by size:
6145 554 442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7141
XmaI C'CCGG_G
Cuts at: 1870 2581 4239 4466 1870
Size: 711 1658 227 4545
Fragments arranged by size:
4545 1658 711 227
XmnI GAAnn'nnTTC
Cuts at: 3988 5270 3988
Size: 1282 5859
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4949 276
Size: 53 83 186 936 3415 2468
Fragments arranged by size:
3415 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SwaI