Restriction map for
pFN21AE9422
(Circular) (Six-base) MAPSORT of: pFN21AE9422.seq Check: 69 from: 1 to: 6775
pFN21AE9422 6775 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4585 278
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4118 1937
Size: 2181 4594
Acc65I G'GTAC_C
Cuts at: 4096 4096
Size: 6775
AclI AA'CG_TT
Cuts at: 1563 4902 5275 1563
Size: 3339 373 3063
Fragments arranged by size:
3339 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2257 2988 3000 3144 3343 4838 6222 6490
Size: 625 731 12 144 199 1495 1384 268
Cuts at: 6490 1632
Size: 1917
Fragments arranged by size:
1917 1495 1384 731 625 268 199 144
12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6578
AflIII A'CryG_T
Cuts at: 1203 5674 6651 1203
Size: 4471 977 1327
Fragments arranged by size:
4471 1327 977
AgeI A'CCGG_T
Cuts at: 5606 5606
Size: 6775
AhdI GACnn_n'nnGTC
Cuts at: 2082 5504 2082
Size: 3422 3353
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3544 1184
Size: 2360 4415
AlwNI CAG_nnn'CTG
Cuts at: 4518 6090 4518
Size: 1572 5203
ApaLI G'TGCA_C
Cuts at: 3920 4832 5988 3920
Size: 912 1156 4707
Fragments arranged by size:
4707 1156 912
ApoI r'AATT_y
Cuts at: 1493 2194 2449 2582 2749 3366 3598 4084 4310
Size: 701 255 133 167 617 232 486 226
Cuts at: 4310 4462 6571 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 701 617 486 255 232 226
167 152 133
AseI AT'TA_AT
Cuts at: 160 1032 2382 5329 160
Size: 872 1350 2947 1606
Fragments arranged by size:
2947 1606 1350 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
AvaI C'yCGr_G
Cuts at: 1870 1943 4100 1870
Size: 73 2157 4545
Fragments arranged by size:
4545 2157 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2610 2643 2610
Size: 33 6742
BamHI G'GATC_C
Cuts at: 4105 4472 4105
Size: 367 6408
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3887 4096 5551 618
Size: 325 217 627 2100 209 1455 1842
Fragments arranged by size:
2100 1842 1455 627 325 217 209
BanII G_rGCy'C
Cuts at: 1391 3029 3865 4094 1391
Size: 1638 836 229 4072
Fragments arranged by size:
4072 1638 836 229
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3150 928
Size: 971 1251 4553
Fragments arranged by size:
4553 1251 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4966 5000 1903
Size: 34 3029 34 3678
Fragments arranged by size:
3678 3029 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2611 3653 4667 5877 2611
Size: 1042 1014 1210 3509
Fragments arranged by size:
3509 1210 1042 1014
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6775
BfrBI ATG'CAT
Cuts at: 3339 3339
Size: 6775
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5386 136
Size: 107 122 71 631 605 3714 1525
Fragments arranged by size:
3714 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2461 6770 2461
Size: 4309 2466
BlpI GC'TnA_GC
Cuts at: 3695 4192 3695
Size: 497 6278
Bme1580I G_kGCm'C
Cuts at: 1790 3924 4836 5992 1790
Size: 2134 912 1156 2573
Fragments arranged by size:
2573 2134 1156 912
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6775
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4506 5464 442
Size: 416 53 83 399 24 3089 958 1753
Fragments arranged by size:
3089 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6775
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3881
Size: 6775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5435 1239
Size: 4196 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4900 5765 6063 4900
Size: 865 298 5612
Fragments arranged by size:
5612 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3185 5438 882
Size: 617 1686 2253 2219
Fragments arranged by size:
2253 2219 1686 617
BsaAI yAC'GTr
Cuts at: 493 1204 4566 493
Size: 711 3362 2702
Fragments arranged by size:
3362 2702 711
BsaBI GATnn'nnATC
Cuts at: 6769 6769
Size: 6775
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4582 4964 5599 275
Size: 53 83 186 936 3049 382 635 1451
Fragments arranged by size:
3049 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5208 5606 5880 6027 6438 5208
Size: 398 274 147 411 5545
Fragments arranged by size:
5545 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2070 2100 3097 3127 1178
Size: 30 165 30 667 30 997 30 4826
Fragments arranged by size:
4826 997 667 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2999 3873 1186
Size: 1813 874 4088
Fragments arranged by size:
4088 1813 874
BseYI C'CCAG_C
Cuts at: 3625 5978 6474 3625
Size: 2353 496 3926
Fragments arranged by size:
3926 2353 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6202
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4225 4986 6014 1540
Size: 275 186 2224 761 1028 2301
Fragments arranged by size:
2301 2224 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3029 3924 4836 4921 5992 3029
Size: 895 912 85 1071 3812
Fragments arranged by size:
3812 1071 912 895 85
BsmI GAATG_Cn'
Cuts at: 2344 4286 4379 2344
Size: 1942 93 4740
Fragments arranged by size:
4740 1942 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3029 3865 3924 4094 4836 4921 5992
Size: 399 1239 836 59 170 742 85 1071
Cuts at: 5992 1391
Size: 2174
Fragments arranged by size:
2174 1239 1071 836 742 399 170 85
59
BspEI T'CCGG_A
Cuts at: 6438 6438
Size: 6775
BspHI T'CATG_A
Cuts at: 4664 6394 4664
Size: 1730 5045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4130 844
Size: 3286 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2020 4662 1394
Size: 429 197 2642 3507
Fragments arranged by size:
3507 2642 429 197
BsrDI GCAATG_nn'
Cuts at: 65 1248 2119 2890 3480 3967 5270 5444 65
Size: 1183 871 771 590 487 1303 174 1396
Fragments arranged by size:
1396 1303 1183 871 771 590 487 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5419 5606 1700
Size: 110 3609 187 2869
Fragments arranged by size:
3609 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6775
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6775
BssSI C'ACGA_G
Cuts at: 4835 5847 4835
Size: 1012 5763
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6775
BstBI TT'CG_AA
Cuts at: 5579 5579
Size: 6775
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6775
BstYI r'GATC_y
Cuts at: 1972 2276 2405 2461 3897 4105 4472 4857 4874
Size: 304 129 56 1436 208 367 385 17
Cuts at: 4874 6326 6412 6424 6770 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1436 385 367 346 304 208
129 86 56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3630 513
Size: 552 2565 3658
Fragments arranged by size:
3658 2565 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6036
Fragments arranged by size:
6036 683 56
BtsI GCAGTG_nn'
Cuts at: 3801 4291 5085 5105 3801
Size: 490 794 20 5471
Fragments arranged by size:
5471 794 490 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6775
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2722 2757 529
Size: 35 2158 35 4547
Fragments arranged by size:
4547 2158 35 35
DraI TTT'AAA
Cuts at: 4079 4079
Size: 6775
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5782 809
Size: 507 4466 1802
Fragments arranged by size:
4466 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4222 5111 6740
Size: 54 1425 50 275 18 2392 889 1629
Cuts at: 6740 8
Size: 43
Fragments arranged by size:
2392 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4222 1537
Size: 275 2410 4090
Fragments arranged by size:
4090 2410 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2976 3473 3876 3953 4705 1353
Size: 1623 497 403 77 752 3423
Fragments arranged by size:
3423 1623 752 497 403 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5362 5736 5882 387
Size: 1064 138 3773 374 146 1280
Fragments arranged by size:
3773 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3027 3027
Size: 6775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2257 2988 3000 3144 3343 4838 5435
Size: 393 625 731 12 144 199 1495 597
Cuts at: 5435 6222 6490 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1495 787 731 625 597 393 268
199 144 12
EcoRI G'AATT_C
Cuts at: 4084 4462 4084
Size: 378 6397
EcoRV GAT'ATC
Cuts at: 2712 2712
Size: 6775
FspI TGC'GCA
Cuts at: 5281 5281
Size: 6775
HaeII r_GCGC'y
Cuts at: 1678 5922 1678
Size: 4244 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2068 2100 2627 2650 2659
Size: 32 135 32 496 32 527 23 9
Cuts at: 2659 2682 2905 2937 5416 5448 5490 5522 1373
Size: 23 223 32 2479 32 42 32 2626
Fragments arranged by size:
2626 2479 527 496 223 135 42 32
32 32 32 32 32 23 23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 4119 4365 669
Size: 988 281 2181 246 3079
Fragments arranged by size:
3079 2181 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6775
HpaI GTT'AAC
Cuts at: 4365 4365
Size: 6775
KpnI G_GTAC'C
Cuts at: 4100 4100
Size: 6775
MfeI C'AATT_G
Cuts at: 4374 4374
Size: 6775
MluI A'CGCG_T
Cuts at: 6651 6651
Size: 6775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3619 5889 6073 1305
Size: 2314 2270 184 2007
Fragments arranged by size:
2314 2270 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4953
Fragments arranged by size:
4953 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3526 3560 4733 5092 5251 518
Size: 648 2360 34 1173 359 159 2042
Fragments arranged by size:
2360 2042 1173 648 359 159 34
MspA1I CmG'CkG
Cuts at: 1606 3153 3210 4191 4868 6016 6261 1606
Size: 1547 57 981 677 1148 245 2120
Fragments arranged by size:
2120 1547 1148 981 677 245 57
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6223
NdeI CA'TA_TG
Cuts at: 387 2817 2869 3335 387
Size: 2430 52 466 3827
Fragments arranged by size:
3827 2430 466 52
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6775
NotI GC'GGCC_GC
Cuts at: 4222 4222
Size: 6775
NsiI A_TGCA'T
Cuts at: 3341 3341
Size: 6775
NspI r_CATG'y
Cuts at: 1132 1229 3741 4133 5678 1132
Size: 97 2512 392 1545 2229
Fragments arranged by size:
2512 2229 1545 392 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6775
PciI A'CATG_T
Cuts at: 5674 5674
Size: 6775
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6775
PmeI GTTT'AAAC
Cuts at: 4079 4079
Size: 6775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4844 4876 6357 6389 4844
Size: 32 1481 32 5230
Fragments arranged by size:
5230 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6775
PsiI TTA'TAA
Cuts at: 2910 3276 4345 2910
Size: 366 1069 5340
Fragments arranged by size:
5340 1069 366
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2653 2685 2653
Size: 32 6743
PstI C_TGCA'G
Cuts at: 830 3808 4127 830
Size: 2978 319 3478
Fragments arranged by size:
3478 2978 319
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6775
PvuII CAG'CTG
Cuts at: 3153 3153
Size: 6775
SacI G_AGCT'C
Cuts at: 3029 3029
Size: 6775
SalI G'TCGA_C
Cuts at: 1936 4117 1936
Size: 2181 4594
SapI GCTCTTCn'nnn_
Cuts at: 3473 3876 3473
Size: 403 6372
SbfI CC_TGCA'GG
Cuts at: 4127 4127
Size: 6775
ScaI AGT'ACT
Cuts at: 5023 6735 5023
Size: 1712 5063
SfcI C'TryA_G
Cuts at: 826 1045 2443 3406 3804 4123 5258 5939 6130
Size: 219 1398 963 398 319 1135 681 191
Cuts at: 6130 826
Size: 1471
Fragments arranged by size:
1471 1398 1135 963 681 398 319 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6775
SmaI CCC'GGG
Cuts at: 1872 4102 1872
Size: 2230 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4879 5780 6042 820
Size: 197 926 2936 901 262 1553
Fragments arranged by size:
2936 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4566 493
Size: 4073 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6775
SphI G_CATG'C
Cuts at: 1132 3741 4133 1132
Size: 2609 392 3774
Fragments arranged by size:
3774 2609 392
SspI AAT'ATT
Cuts at: 5 52 2380 2499 3280 4028 4699 5
Size: 47 2328 119 781 748 671 2081
Fragments arranged by size:
2328 2081 781 748 671 119 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6775
StyI C'CwwG_G
Cuts at: 513 1065 2550 4214 513
Size: 552 1485 1664 3074
Fragments arranged by size:
3074 1664 1485 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3052 3554 4802 4819 4972 5157 5602
Size: 1255 1472 502 1248 17 153 185 445
Cuts at: 5602 325
Size: 1498
Fragments arranged by size:
1498 1472 1255 1248 502 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5021 6733 96
Size: 274 80 33 51 1443 3044 1712 138
Fragments arranged by size:
3044 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2070 2102 3097 3129 6735 6767 2070
Size: 32 995 32 3606 32 2078
Fragments arranged by size:
3606 2078 995 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6775
XbaI T'CTAG_A
Cuts at: 4007 4111 4007
Size: 104 6671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6775
XmaI C'CCGG_G
Cuts at: 1870 4100 1870
Size: 2230 4545
XmnI GAAnn'nnTTC
Cuts at: 4904 4904
Size: 6775
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4583 276
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI