Restriction map for
pFN21AE9421
(Circular) (Six-base) MAPSORT of: pFN21AE9421.seq Check: 8812 from: 1 to: 5749
pFN21AE9421 5749 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3092 1937
Size: 1155 4594
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2251 3051 3812 5196 5464 1632
Size: 619 800 761 1384 268 1917
Fragments arranged by size:
1917 1384 800 761 619 268
AflII C'TTAA_G
Cuts at: 820 1017 2995 820
Size: 197 1978 3574
Fragments arranged by size:
3574 1978 197
AflIII A'CryG_T
Cuts at: 1203 2330 4648 5625 1203
Size: 1127 2318 977 1327
Fragments arranged by size:
2318 1327 1127 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2003 2035 2003
Size: 32 5717
AlwNI CAG_nnn'CTG
Cuts at: 2316 3492 5064 2316
Size: 1176 1572 3001
Fragments arranged by size:
3001 1572 1176
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 2321 2857 3058 3284 3436 5545 1493
Size: 828 536 201 226 152 2109 1697
Fragments arranged by size:
2109 1697 828 536 226 201 152
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 3074 1870
Size: 73 1131 4545
Fragments arranged by size:
4545 1131 73
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3070 4525 618
Size: 325 217 627 1283 1455 1842
Fragments arranged by size:
1842 1455 1283 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3068 1391
Size: 1677 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4778
BbvCI CC'TCA_GC
Cuts at: 2028 2028
Size: 5749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3940 3974 1903
Size: 34 2003 34 3678
Fragments arranged by size:
3678 2003 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BfrBI ATG'CAT
Cuts at: 2460 2460
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4360 136
Size: 107 122 71 631 605 2688 1525
Fragments arranged by size:
2688 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 3810 4966 1790
Size: 2020 1156 2573
Fragments arranged by size:
2573 2020 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2605 3480 4438
Size: 416 53 83 399 24 1188 875 958
Cuts at: 4438 442
Size: 1753
Fragments arranged by size:
1753 1188 958 875 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4409 1239
Size: 3170 2579
Bpu10I CC'TnA_GC
Cuts at: 2028 2028
Size: 5749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3874 4739 5037 3874
Size: 865 298 4586
Fragments arranged by size:
4586 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4412 882
Size: 617 2913 2219
Fragments arranged by size:
2913 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3540 493
Size: 711 2336 2702
Fragments arranged by size:
2702 2336 711
BsaBI GATnn'nnATC
Cuts at: 2171 5743 2171
Size: 3572 2177
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3556 3938 4573 275
Size: 53 83 186 936 2023 382 635 1451
Fragments arranged by size:
2023 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 2241 2271 1178
Size: 30 165 30 600 30 208 30 4656
Fragments arranged by size:
4656 600 208 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2716 2859 1186
Size: 1530 143 4076
Fragments arranged by size:
4076 1530 143
BseYI C'CCAG_C
Cuts at: 4952 5448 4952
Size: 496 5253
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2177 1163
Size: 573 441 4735
Fragments arranged by size:
4735 573 441
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3199 3960 4988 1540
Size: 275 186 1198 761 1028 2301
Fragments arranged by size:
2301 1198 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3810 3895 4966 3810
Size: 85 1071 4593
Fragments arranged by size:
4593 1071 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3068 3810 3895 4966 1391
Size: 399 1278 742 85 1071 2174
Fragments arranged by size:
2174 1278 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 2163 3638 5368 2163
Size: 1475 1730 2544
Fragments arranged by size:
2544 1730 1475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3104 844
Size: 2260 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3636 1394
Size: 429 1813 3507
Fragments arranged by size:
3507 1813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2397 4244 4418 65
Size: 1183 1149 1847 174 1396
Fragments arranged by size:
1847 1396 1183 1149 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4393 4580 1700
Size: 110 2583 187 2869
Fragments arranged by size:
2869 2583 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 2782 2957 2782
Size: 175 5574
BstYI r'GATC_y
Cuts at: 1972 3079 3446 3831 3848 5300 5386 5398 5744
Size: 1107 367 385 17 1452 86 12 346
Cuts at: 5744 1972
Size: 1977
Fragments arranged by size:
1977 1452 1107 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2640 513
Size: 552 939 636 3622
Fragments arranged by size:
3622 939 636 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 2844 3265 4059 4079 2844
Size: 421 794 20 4514
Fragments arranged by size:
4514 794 421 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3007 4756 809
Size: 507 1691 1749 1802
Fragments arranged by size:
1802 1749 1691 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2673 3196 4085
Size: 54 1425 50 275 18 843 523 889
Cuts at: 4085 5714 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 843 523 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3679 1353
Size: 2326 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2251 3051 3812 4409 5196 5464 1239
Size: 393 619 800 761 597 787 268 1524
Fragments arranged by size:
1524 800 787 761 619 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2093 2599 2093
Size: 506 5243
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 4896 1678
Size: 3218 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2091 2123 4390 4422 4464
Size: 32 135 32 519 32 2267 32 42
Cuts at: 4464 4496 1373
Size: 32 2626
Fragments arranged by size:
2626 2267 519 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3093 3339 669
Size: 988 281 1074 81 246 3079
Fragments arranged by size:
3079 1074 988 281 246 81
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2450 4863 5047 1305
Size: 1145 2413 184 2007
Fragments arranged by size:
2413 2007 1145 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2675 10
Size: 54 1425 343 843 3084
Fragments arranged by size:
3084 1425 843 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 3707 4066 4225 518
Size: 648 1092 1449 359 159 2042
Fragments arranged by size:
2042 1449 1092 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3165 3842 4990 5235 1606
Size: 1559 677 1148 245 2120
Fragments arranged by size:
2120 1559 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2640 513
Size: 552 939 636 3622
Fragments arranged by size:
3622 939 636 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NsiI A_TGCA'T
Cuts at: 2462 2462
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2334 3107 4652 1132
Size: 97 1105 773 1545 2229
Fragments arranged by size:
2229 1545 1105 773 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5749
PciI A'CATG_T
Cuts at: 2330 4648 2330
Size: 2318 3431
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5749
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2003 2035 3818 3850 5331 5363 2003
Size: 32 1783 32 1481 32 2389
Fragments arranged by size:
2389 1783 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2093 2599 2093
Size: 506 5243
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5749
PsiI TTA'TAA
Cuts at: 2864 2910 3319 2864
Size: 46 409 5294
Fragments arranged by size:
5294 409 46
PstI C_TGCA'G
Cuts at: 830 3101 830
Size: 2271 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SfcI C'TryA_G
Cuts at: 826 1045 3097 4232 4913 5104 826
Size: 219 2052 1135 681 191 1471
Fragments arranged by size:
2052 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 3076 1872
Size: 1204 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2995 3853 4754 5016 820
Size: 197 926 1052 858 901 262 1553
Fragments arranged by size:
1553 1052 926 901 858 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5749
StyI C'CwwG_G
Cuts at: 513 1065 2004 2119 2640 2781 3188 513
Size: 552 939 115 521 141 407 3074
Fragments arranged by size:
3074 939 552 521 407 141 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3776 3793 3946 4131 4576 325
Size: 1255 2196 17 153 185 445 1498
Fragments arranged by size:
2196 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2702 3995 5707
Size: 274 80 33 51 1443 725 1293 1712
Cuts at: 5707 96
Size: 138
Fragments arranged by size:
1712 1443 1293 725 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2241 2273 5709 5741 2241
Size: 32 3436 32 2249
Fragments arranged by size:
3436 2249 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2638 2638
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 3074 1870
Size: 1204 4545
XmnI GAAnn'nnTTC
Cuts at: 2015 3878 2015
Size: 1863 3886
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI