Restriction map for
pFN21AE9419
(Circular) (Six-base) MAPSORT of: pFN21AE9419.seq Check: 7108 from: 1 to: 6112
pFN21AE9419 6112 bp
With 182 enzymes: SgfI *
September 21, 2017 12:07 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2492 3922 278
Size: 53 83 186 936 956 1430 2468
Fragments arranged by size:
2468 1430 956 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3108 3366 3455 1937
Size: 1171 258 89 4594
Fragments arranged by size:
4594 1171 258 89
Acc65I G'GTAC_C
Cuts at: 3433 3433
Size: 6112
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2062 2588 3064 4175 5559 5827 1632
Size: 430 526 476 1111 1384 268 1917
Fragments arranged by size:
1917 1384 1111 526 476 430 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 2124 2499 3111 5011 5988 1203
Size: 921 375 612 1900 977 1327
Fragments arranged by size:
1900 1327 977 921 612 375
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6112
AlwNI CAG_nnn'CTG
Cuts at: 2634 3295 3855 5427 2634
Size: 661 560 1572 3319
Fragments arranged by size:
3319 1572 661 560
ApaLI G'TGCA_C
Cuts at: 4169 5325 4169
Size: 1156 4956
ApoI r'AATT_y
Cuts at: 1493 3421 3647 3799 5908 1493
Size: 1928 226 152 2109 1697
Fragments arranged by size:
2109 1928 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2181 2181
Size: 6112
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 2200 2238 2583 3437 1870
Size: 73 257 38 345 854 4545
Fragments arranged by size:
4545 854 345 257 73 38
BamHI G'GATC_C
Cuts at: 3442 3809 3442
Size: 367 5745
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3073 3307 3433 4888 618
Size: 325 217 627 1286 234 126 1455 1842
Fragments arranged by size:
1842 1455 1286 627 325 234 217 126
BanII G_rGCy'C
Cuts at: 1391 3431 1391
Size: 2040 4072
BbeI G_GCGC'C
Cuts at: 3311 3311
Size: 6112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4303 4337 1903
Size: 34 50 34 2282 34 3678
Fragments arranged by size:
3678 2282 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4004 5214 4004
Size: 1210 4902
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2684 4723 136
Size: 107 122 71 631 605 1012 2039 1525
Fragments arranged by size:
2039 1525 1012 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 3529 3529
Size: 6112
Bme1580I G_kGCm'C
Cuts at: 1790 3078 4173 5329 1790
Size: 1288 1095 1156 2573
Fragments arranged by size:
2573 1288 1156 1095
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2278 2691 3843
Size: 416 53 83 399 24 861 413 1152
Cuts at: 3843 4801 442
Size: 958 1753
Fragments arranged by size:
1753 1152 958 861 416 413 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2069 1056
Size: 1013 5099
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 2186 3067 4772 1239
Size: 881 66 881 1705 2579
Fragments arranged by size:
2579 1705 881 881 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3188 4237 5102 5400 3188
Size: 1049 865 298 3900
Fragments arranged by size:
3900 1049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4775 882
Size: 617 3276 2219
Fragments arranged by size:
3276 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3903 493
Size: 711 2699 2702
Fragments arranged by size:
2702 2699 711
BsaBI GATnn'nnATC
Cuts at: 2252 6106 2252
Size: 3854 2258
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2489 2938 3308 3357
Size: 53 83 186 936 956 449 370 49
Cuts at: 3357 3919 4301 4936 275
Size: 562 382 635 1451
Fragments arranged by size:
1451 956 936 635 562 449 382 370
186 83 53 49
BsaWI w'CCGG_w
Cuts at: 2140 2577 2945 4545 4943 5217 5364 5775 2140
Size: 437 368 1600 398 274 147 411 2477
Fragments arranged by size:
2477 1600 437 411 398 368 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5887
Fragments arranged by size:
5887 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2750 1186
Size: 1564 4548
BseYI C'CCAG_C
Cuts at: 2158 2770 2794 2824 2842 3395 5315 5811 2158
Size: 612 24 30 18 553 1920 496 2459
Fragments arranged by size:
2459 1920 612 553 496 30 24 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5539
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2596 2614 2893 2946 3324 3562
Size: 275 186 595 18 279 53 378 238
Cuts at: 3562 4323 5351 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 595 378 279 275 238
186 53 18
BsiHKAI G_wGCw'C
Cuts at: 2403 3282 4173 4258 5329 2403
Size: 879 891 85 1071 3186
Fragments arranged by size:
3186 1071 891 879 85
BsmI GAATG_Cn'
Cuts at: 3623 3716 3623
Size: 93 6019
BsmBI CGTCTCn'nnnn_
Cuts at: 3234 3234
Size: 6112
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2403 3078 3282 3431 4173 4258 5329
Size: 399 613 675 204 149 742 85 1071
Cuts at: 5329 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 675 613 399 204 149
85
BspEI T'CCGG_A
Cuts at: 2140 5775 2140
Size: 3635 2477
BspHI T'CATG_A
Cuts at: 4001 5731 4001
Size: 1730 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2310 2874 3345 3396 3467 844
Size: 1466 564 471 51 71 3489
Fragments arranged by size:
3489 1466 564 471 71 51
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4607 4781 65
Size: 1183 3359 174 1396
Fragments arranged by size:
3359 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4756 4943 1700
Size: 110 2946 187 2869
Fragments arranged by size:
2946 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 2181 1925
Size: 256 5856
BssSI C'ACGA_G
Cuts at: 2113 3176 3281 4172 5184 2113
Size: 1063 105 891 1012 3041
Fragments arranged by size:
3041 1063 1012 891 105
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2818 794
Size: 2024 4088
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 2974 1184
Size: 1790 4322
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6112
BstYI r'GATC_y
Cuts at: 1972 2664 3442 3809 4194 4211 5663 5749 5761
Size: 692 778 367 385 17 1452 86 12
Cuts at: 5761 6107 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 778 692 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3109 3109
Size: 6112
BtgI C'CryG_G
Cuts at: 513 1065 2481 2715 2922 3324 513
Size: 552 1416 234 207 402 3301
Fragments arranged by size:
3301 1416 552 402 234 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2869 3208 487
Size: 683 56 1643 339 3391
Fragments arranged by size:
3391 1643 683 339 56
BtsI GCAGTG_nn'
Cuts at: 2523 3628 4422 4442 2523
Size: 1105 794 20 4193
Fragments arranged by size:
4193 1105 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2098 2133 529
Size: 35 1534 35 4508
Fragments arranged by size:
4508 1534 35 35
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5119 809
Size: 507 3803 1802
Fragments arranged by size:
3803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2106 2593
Size: 54 1425 50 275 18 183 93 487
Cuts at: 2593 2611 2643 2676 3321 3559 4448 6077 8
Size: 18 32 33 645 238 889 1629 43
Fragments arranged by size:
1629 1425 889 645 487 275 238 183
93 54 50 43 33 32 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2593 2611 3321 3559 1537
Size: 275 781 18 710 238 4090
Fragments arranged by size:
4090 781 710 275 238 18
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2318 2366 2564 4042 1353
Size: 728 237 48 198 1478 3423
Fragments arranged by size:
3423 1478 728 237 198 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4699 5073 5219 387
Size: 1064 138 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2120 2186 2588 3064 3067 4175
Size: 393 430 58 66 402 476 3 1108
Cuts at: 4175 4772 5559 5827 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1108 787 597 476 430 402 393
268 66 58 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2099 3101 2099
Size: 1002 5110
EcoO109I rG'GnC_Cy
Cuts at: 3301 3301
Size: 6112
EcoRI G'AATT_C
Cuts at: 3421 3799 3421
Size: 378 5734
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 2381 2654 2958 3038 3203 3311 3382 5259
Size: 703 273 304 80 165 108 71 1877
Cuts at: 5259 1678
Size: 2531
Fragments arranged by size:
2531 1877 703 304 273 165 108 80
71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4753 4785 4827 4859 1373
Size: 32 135 32 3181 32 42 32 2626
Fragments arranged by size:
3181 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3456 3702 669
Size: 988 281 1518 246 3079
Fragments arranged by size:
3079 1518 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6112
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KasI G'GCGC_C
Cuts at: 3307 3307
Size: 6112
KpnI G_GTAC'C
Cuts at: 3437 3437
Size: 6112
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 2124 3111 5988 2124
Size: 987 2877 2248
Fragments arranged by size:
2877 2248 987
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3168 5226 5410 1305
Size: 1863 2058 184 2007
Fragments arranged by size:
2058 2007 1863 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4290
Fragments arranged by size:
4290 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2258 2498 2528 2534 3245 4070
Size: 648 843 249 240 30 6 711 825
Cuts at: 4070 4429 4588 518
Size: 359 159 2042
Fragments arranged by size:
2042 843 825 711 648 359 249 240
159 30 6
MspA1I CmG'CkG
Cuts at: 1606 2208 2631 2842 2924 3150 3326 3372 3528
Size: 602 423 211 82 226 176 46 156
Cuts at: 3528 4205 5353 5598 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 602 423 245 226 211
176 156 82 46
NarI GG'CG_CC
Cuts at: 3308 3308
Size: 6112
NcoI C'CATG_G
Cuts at: 513 1065 2481 513
Size: 552 1416 4144
Fragments arranged by size:
4144 1416 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 2065 1052
Size: 1013 5099
NotI GC'GGCC_GC
Cuts at: 3321 3559 3321
Size: 238 5874
NspI r_CATG'y
Cuts at: 1132 1229 2332 2503 3250 3470 5015 1132
Size: 97 1103 171 747 220 1545 2229
Fragments arranged by size:
2229 1545 1103 747 220 171 97
PasI CC'CwG_GG
Cuts at: 1348 2284 1348
Size: 936 5176
PciI A'CATG_T
Cuts at: 2499 5011 2499
Size: 2512 3600
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2554 1108
Size: 1446 4666
PfoI T'CCnGG_A
Cuts at: 2088 2757 2088
Size: 669 5443
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6112
PsiI TTA'TAA
Cuts at: 3682 3682
Size: 6112
PstI C_TGCA'G
Cuts at: 830 2136 3300 3464 830
Size: 1306 1164 164 3478
Fragments arranged by size:
3478 1306 1164 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
PvuII CAG'CTG
Cuts at: 2631 3150 2631
Size: 519 5593
SacII CC_GC'GG
Cuts at: 2925 3327 2925
Size: 402 5710
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SapI GCTCTTCn'nnn_
Cuts at: 2318 2564 2318
Size: 246 5866
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SfcI C'TryA_G
Cuts at: 826 1045 2132 3296 3367 3460 4595 5276 5467
Size: 219 1087 1164 71 93 1135 681 191
Cuts at: 5467 826
Size: 1471
Fragments arranged by size:
1471 1164 1135 1087 681 219 191 93
71
SfiI GGCCn_nnn'nGGCC
Cuts at: 2684 2684
Size: 6112
SfoI GGC'GCC
Cuts at: 3309 3309
Size: 6112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 2202 2240 3439 1872
Size: 330 38 1199 4545
Fragments arranged by size:
4545 1199 330 38
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3203 4216 5117 5379 820
Size: 197 926 1260 1013 901 262 1553
Fragments arranged by size:
1553 1260 1013 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 2332 3250 3470 1132
Size: 1200 918 220 3774
Fragments arranged by size:
3774 1200 918 220
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6112
StyI C'CwwG_G
Cuts at: 513 1065 2481 2646 3551 513
Size: 552 1416 165 905 3074
Fragments arranged by size:
3074 1416 905 552 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2260 4139 4156 4309 4494 4939 325
Size: 1255 680 1879 17 153 185 445 1498
Fragments arranged by size:
1879 1498 1255 680 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2265 4358 6070
Size: 274 80 33 51 1443 288 2093 1712
Cuts at: 6070 96
Size: 138
Fragments arranged by size:
2093 1712 1443 288 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2135 2167 6072 6104 2135
Size: 32 3905 32 2143
Fragments arranged by size:
3905 2143 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6112
XbaI T'CTAG_A
Cuts at: 3448 3448
Size: 6112
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 2200 2238 3437 1870
Size: 330 38 1199 4545
Fragments arranged by size:
4545 1199 330 38
XmnI GAAnn'nnTTC
Cuts at: 4241 4241
Size: 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2490 3920 276
Size: 53 83 186 936 956 1430 2468
Fragments arranged by size:
2468 1430 956 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI
SrfI SwaI XcmI