Restriction map for
pFN21AE9418
(Circular) (Six-base) MAPSORT of: pFN21AE9418.seq Check: 2374 from: 1 to: 5839
pFN21AE9418 5839 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3649 278
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2334 3182 1937
Size: 397 848 4594
Fragments arranged by size:
4594 848 397
Acc65I G'GTAC_C
Cuts at: 3160 3160
Size: 5839
AclI AA'CG_TT
Cuts at: 1563 3966 4339 1563
Size: 2403 373 3063
Fragments arranged by size:
3063 2403 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2999 3902 5286 5554 1632
Size: 1367 903 1384 268 1917
Fragments arranged by size:
1917 1384 1367 903 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 2653 4738 5715 1203
Size: 1450 2085 977 1327
Fragments arranged by size:
2085 1450 1327 977
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 4568 4568
Size: 5839
AleI CACnn'nnGTG
Cuts at: 1166 2103 1166
Size: 937 4902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5839
AlwNI CAG_nnn'CTG
Cuts at: 2221 3582 5154 2221
Size: 1361 1572 2906
Fragments arranged by size:
2906 1572 1361
ApaI G_GGCC'C
Cuts at: 2836 2836
Size: 5839
ApaLI G'TGCA_C
Cuts at: 3896 5052 3896
Size: 1156 4683
ApoI r'AATT_y
Cuts at: 1493 2019 3148 3374 3526 5635 1493
Size: 526 1129 226 152 2109 1697
Fragments arranged by size:
2109 1697 1129 526 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 3164 1870
Size: 73 1221 4545
Fragments arranged by size:
4545 1221 73
BamHI G'GATC_C
Cuts at: 3169 3536 3169
Size: 367 5472
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2851 3160 4615 618
Size: 325 217 627 1064 309 1455 1842
Fragments arranged by size:
1842 1455 1064 627 325 309 217
BanII G_rGCy'C
Cuts at: 1391 2694 2836 3158 1391
Size: 1303 142 322 4072
Fragments arranged by size:
4072 1303 322 142
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2284 928
Size: 971 385 4483
Fragments arranged by size:
4483 971 385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4030 4064 1903
Size: 34 2093 34 3678
Fragments arranged by size:
3678 2093 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3731 4941 3731
Size: 1210 4629
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5839
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2246 4450 136
Size: 107 122 71 631 605 574 2204 1525
Fragments arranged by size:
2204 1525 631 605 574 122 107 71
BglII A'GATC_T
Cuts at: 2805 5834 2805
Size: 3029 2810
BlpI GC'TnA_GC
Cuts at: 3256 3256
Size: 5839
Bme1580I G_kGCm'C
Cuts at: 1790 2836 3900 5056 1790
Size: 1046 1064 1156 2573
Fragments arranged by size:
2573 1156 1064 1046
BmgBI CAC'GTC
Cuts at: 1318 2362 1318
Size: 1044 4795
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3570 4528 442
Size: 416 53 83 399 24 2153 958 1753
Fragments arranged by size:
2153 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2754 3017 2754
Size: 263 5576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4499 1239
Size: 3260 2579
Bpu10I CC'TnA_GC
Cuts at: 2843 2843
Size: 5839
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3964 4829 5127 3964
Size: 865 298 4676
Fragments arranged by size:
4676 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2263 4502 882
Size: 617 764 2239 2219
Fragments arranged by size:
2239 2219 764 617
BsaAI yAC'GTr
Cuts at: 493 1204 3630 493
Size: 711 2426 2702
Fragments arranged by size:
2702 2426 711
BsaBI GATnn'nnATC
Cuts at: 5833 5833
Size: 5839
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 3646 4028 4663
Size: 53 83 186 936 618 1495 382 635
Cuts at: 4663 275
Size: 1451
Fragments arranged by size:
1495 1451 936 635 618 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4272 4670 4944 5091 5502 4272
Size: 398 274 147 411 4609
Fragments arranged by size:
4609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5614
Fragments arranged by size:
5614 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5839
BseYI C'CCAG_C
Cuts at: 2766 5042 5538 2766
Size: 2276 496 3067
Fragments arranged by size:
3067 2276 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2569 2822 1163
Size: 573 833 253 4180
Fragments arranged by size:
4180 833 573 253
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3289 4050 5078 1540
Size: 275 186 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3120 3900 3985 5056 3120
Size: 780 85 1071 3903
Fragments arranged by size:
3903 1071 780 85
BsmI GAATG_Cn'
Cuts at: 3350 3443 3350
Size: 93 5746
BsmBI CGTCTCn'nnnn_
Cuts at: 2162 2162
Size: 5839
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2694 2836 3120 3158 3900 3985 5056
Size: 399 904 142 284 38 742 85 1071
Cuts at: 5056 1391
Size: 2174
Fragments arranged by size:
2174 1071 904 742 399 284 142 85
38
BspEI T'CCGG_A
Cuts at: 5502 5502
Size: 5839
BspHI T'CATG_A
Cuts at: 3728 5458 3728
Size: 1730 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3194 844
Size: 2350 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2515 3726 1394
Size: 429 692 1211 3507
Fragments arranged by size:
3507 1211 692 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2235 4334 4508 65
Size: 1183 987 2099 174 1396
Fragments arranged by size:
2099 1396 1183 987 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4483 4670 1700
Size: 110 2673 187 2869
Fragments arranged by size:
2869 2673 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5839
BssSI C'ACGA_G
Cuts at: 3899 4911 3899
Size: 1012 4827
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2221 794
Size: 1427 4412
BstBI TT'CG_AA
Cuts at: 4643 4643
Size: 5839
BstEII G'GTnAC_C
Cuts at: 1184 2105 1184
Size: 921 4918
BstXI CCAn_nnnn'nTGG
Cuts at: 2042 2042
Size: 5839
BstYI r'GATC_y
Cuts at: 1972 2805 3169 3536 3921 3938 5390 5476 5488
Size: 833 364 367 385 17 1452 86 12
Cuts at: 5488 5834 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 833 385 367 364 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2335 2335
Size: 5839
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4348
Fragments arranged by size:
4348 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5100
Fragments arranged by size:
5100 683 56
BtsI GCAGTG_nn'
Cuts at: 2211 2391 3355 4149 4169 2211
Size: 180 964 794 20 3881
Fragments arranged by size:
3881 964 794 180 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5804
DraI TTT'AAA
Cuts at: 3143 3143
Size: 5839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2451 2499 3286
Size: 54 1425 50 275 18 621 48 787
Cuts at: 3286 4175 5804 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 787 621 275 54 50
48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3286 1537
Size: 275 1474 4090
Fragments arranged by size:
4090 1474 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2537 2699 3769 1353
Size: 1184 162 1070 3423
Fragments arranged by size:
3423 1184 1070 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4426 4800 4946 387
Size: 1064 138 2837 374 146 1280
Fragments arranged by size:
2837 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2999 3902 4499 5286 5554 1239
Size: 393 1367 903 597 787 268 1524
Fragments arranged by size:
1524 1367 903 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2218 2599 2833 2218
Size: 381 234 5224
Fragments arranged by size:
5224 381 234
EcoRI G'AATT_C
Cuts at: 3148 3526 3148
Size: 378 5461
EcoRV GAT'ATC
Cuts at: 2073 2073
Size: 5839
FspI TGC'GCA
Cuts at: 4345 4345
Size: 5839
HaeII r_GCGC'y
Cuts at: 1678 2931 4986 1678
Size: 1253 2055 2531
Fragments arranged by size:
2531 2055 1253
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4480 4512 4554 4586 1373
Size: 32 135 32 2908 32 42 32 2626
Fragments arranged by size:
2908 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 3429 669
Size: 988 281 1245 246 3079
Fragments arranged by size:
3079 1245 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2681 748
Size: 1933 3906
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KpnI G_GTAC'C
Cuts at: 3164 3164
Size: 5839
MfeI C'AATT_G
Cuts at: 3438 3438
Size: 5839
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3028 4953 5137 1305
Size: 1723 1925 184 2007
Fragments arranged by size:
2007 1925 1723 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2453 2501 10
Size: 54 1425 343 621 48 3348
Fragments arranged by size:
3348 1425 621 343 54 48
MslI CAynn'nnrTG
Cuts at: 518 1166 2103 2186 2204 3797 4156 4315 518
Size: 648 937 83 18 1593 359 159 2042
Fragments arranged by size:
2042 1593 937 648 359 159 83 18
MspA1I CmG'CkG
Cuts at: 1606 3053 3255 3932 5080 5325 1606
Size: 1447 202 677 1148 245 2120
Fragments arranged by size:
2120 1447 1148 677 245 202
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4348
Fragments arranged by size:
4348 939 552
NdeI CA'TA_TG
Cuts at: 387 2187 387
Size: 1800 4039
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 2657 2667 3095 3197 4742 1132
Size: 97 1428 10 428 102 1545 2229
Fragments arranged by size:
2229 1545 1428 428 102 97 10
PasI CC'CwG_GG
Cuts at: 1348 3111 1348
Size: 1763 4076
PciI A'CATG_T
Cuts at: 2653 4738 2653
Size: 2085 3754
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4937
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 5421 5453 3908
Size: 32 1481 32 4294
Fragments arranged by size:
4294 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5839
PsiI TTA'TAA
Cuts at: 2918 3409 2918
Size: 491 5348
PspOMI G'GGCC_C
Cuts at: 2832 2832
Size: 5839
PstI C_TGCA'G
Cuts at: 830 3191 830
Size: 2361 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5839
PvuII CAG'CTG
Cuts at: 3053 3053
Size: 5839
SalI G'TCGA_C
Cuts at: 1936 3181 1936
Size: 1245 4594
SapI GCTCTTCn'nnn_
Cuts at: 2699 2699
Size: 5839
SbfI CC_TGCA'GG
Cuts at: 3191 3191
Size: 5839
ScaI AGT'ACT
Cuts at: 4087 5799 4087
Size: 1712 4127
SexAI A'CCwGG_T
Cuts at: 2130 2346 2130
Size: 216 5623
SfcI C'TryA_G
Cuts at: 826 1045 3187 4322 5003 5194 826
Size: 219 2142 1135 681 191 1471
Fragments arranged by size:
2142 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 3166 1872
Size: 1294 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3943 4844 5106 820
Size: 197 926 2000 901 262 1553
Fragments arranged by size:
2000 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3630 493
Size: 3137 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5839
SphI G_CATG'C
Cuts at: 1132 3197 1132
Size: 2065 3774
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5839
StyI C'CwwG_G
Cuts at: 513 1065 2004 2244 3278 513
Size: 552 939 240 1034 3074
Fragments arranged by size:
3074 1034 939 552 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3866 3883 4036 4221 4666 325
Size: 1255 2286 17 153 185 445 1498
Fragments arranged by size:
2286 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4085 5797 96
Size: 274 80 33 51 1443 2108 1712 138
Fragments arranged by size:
2108 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2037 2069 5799 5831 2037
Size: 32 3730 32 2045
Fragments arranged by size:
3730 2045 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5839
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2251 2251
Size: 5839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 3164 1870
Size: 1294 4545
XmnI GAAnn'nnTTC
Cuts at: 3968 3968
Size: 5839
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3647 276
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI Bsu36I DraIII EcoICRI EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI