Restriction map for
pFN21AE9416
(Circular) (Six-base) MAPSORT of: pFN21AE9416.seq Check: 1598 from: 1 to: 6325
pFN21AE9416 6325 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4135 278
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2631 3668 1937
Size: 694 1037 4594
Fragments arranged by size:
4594 1037 694
Acc65I G'GTAC_C
Cuts at: 3646 3646
Size: 6325
AclI AA'CG_TT
Cuts at: 1563 4452 4825 1563
Size: 2889 373 3063
Fragments arranged by size:
3063 2889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2009 2087 2203 2308 2423 3579 4388 5772
Size: 377 78 116 105 115 1156 809 1384
Cuts at: 5772 6040 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1156 809 377 268 116 115
105 78
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6128
AflIII A'CryG_T
Cuts at: 1203 3297 5224 6201 1203
Size: 2094 1927 977 1327
Fragments arranged by size:
2094 1927 1327 977
AgeI A'CCGG_T
Cuts at: 5156 5156
Size: 6325
AhdI GACnn_n'nnGTC
Cuts at: 5054 5054
Size: 6325
AleI CACnn'nnGTG
Cuts at: 1166 2129 2312 2972 1166
Size: 963 183 660 4519
Fragments arranged by size:
4519 963 660 183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6325
AlwNI CAG_nnn'CTG
Cuts at: 2689 4068 5640 2689
Size: 1379 1572 3374
Fragments arranged by size:
3374 1572 1379
ApaLI G'TGCA_C
Cuts at: 4382 5538 4382
Size: 1156 5169
ApoI r'AATT_y
Cuts at: 1493 2225 3323 3634 3860 4012 6121 1493
Size: 732 1098 311 226 152 2109 1697
Fragments arranged by size:
2109 1697 1098 732 311 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4879 160
Size: 872 3847 1606
Fragments arranged by size:
3847 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
AvaI C'yCGr_G
Cuts at: 1870 1943 3650 1870
Size: 73 1707 4545
Fragments arranged by size:
4545 1707 73
BamHI G'GATC_C
Cuts at: 3655 4022 3655
Size: 367 5958
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3171 3646 5101 618
Size: 325 217 627 1384 475 1455 1842
Fragments arranged by size:
1842 1455 1384 627 475 325 217
BanII G_rGCy'C
Cuts at: 1391 3644 1391
Size: 2253 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2093 2185 2411 2564 2999 928
Size: 971 194 92 226 153 435 4254
Fragments arranged by size:
4254 971 435 226 194 153 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4516 4550 1903
Size: 34 2579 34 3678
Fragments arranged by size:
3678 2579 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2351 4217 5427 2351
Size: 1866 1210 3249
Fragments arranged by size:
3249 1866 1210
BclI T'GATC_A
Cuts at: 1548 2976 1548
Size: 1428 4897
BfrBI ATG'CAT
Cuts at: 3550 3550
Size: 6325
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2537 2810 4936
Size: 107 122 71 631 605 865 273 2126
Cuts at: 4936 136
Size: 1525
Fragments arranged by size:
2126 1525 865 631 605 273 122 107
71
BglII A'GATC_T
Cuts at: 3381 6320 3381
Size: 2939 3386
BlpI GC'TnA_GC
Cuts at: 2329 3742 2329
Size: 1413 4912
Bme1580I G_kGCm'C
Cuts at: 1790 2720 4386 5542 1790
Size: 930 1666 1156 2573
Fragments arranged by size:
2573 1666 1156 930
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6325
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3023 4056 5014
Size: 416 53 83 399 24 1606 1033 958
Cuts at: 5014 442
Size: 1753
Fragments arranged by size:
1753 1606 1033 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2897 3028 4985 1239
Size: 836 822 131 1957 2579
Fragments arranged by size:
2579 1957 836 822 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2688 4450 5315 5613 2688
Size: 1762 865 298 3400
Fragments arranged by size:
3400 1762 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2662 4988 882
Size: 617 1163 2326 2219
Fragments arranged by size:
2326 2219 1163 617
BsaAI yAC'GTr
Cuts at: 493 1204 4116 493
Size: 711 2912 2702
Fragments arranged by size:
2912 2702 711
BsaBI GATnn'nnATC
Cuts at: 6319 6319
Size: 6325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4132 4514 5149 275
Size: 53 83 186 936 2599 382 635 1451
Fragments arranged by size:
2599 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2436 4758 5156 5430 5577 5988 2436
Size: 2322 398 274 147 411 2773
Fragments arranged by size:
2773 2322 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3254 3284 1178
Size: 30 165 30 1851 30 4219
Fragments arranged by size:
4219 1851 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2483 3497 1186
Size: 1297 1014 4014
Fragments arranged by size:
4014 1297 1014
BseYI C'CCAG_C
Cuts at: 5528 6024 5528
Size: 496 5829
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2411 2467 2795 3165 3596 1163
Size: 573 675 56 328 370 431 3892
Fragments arranged by size:
3892 675 573 431 370 328 56
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3775 4536 5564 1540
Size: 275 186 1774 761 1028 2301
Fragments arranged by size:
2301 1774 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3168 4386 4471 5542 3168
Size: 1218 85 1071 3951
Fragments arranged by size:
3951 1218 1071 85
BsmI GAATG_Cn'
Cuts at: 3836 3929 3836
Size: 93 6232
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2720 3168 3644 4386 4471 5542 1391
Size: 399 930 448 476 742 85 1071 2174
Fragments arranged by size:
2174 1071 930 742 476 448 399 85
BspEI T'CCGG_A
Cuts at: 2436 5988 2436
Size: 3552 2773
BspHI T'CATG_A
Cuts at: 2247 2673 3277 4214 5944 2247
Size: 426 604 937 1730 2628
Fragments arranged by size:
2628 1730 937 604 426
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3680 844
Size: 2836 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4212 1394
Size: 429 2389 3507
Fragments arranged by size:
3507 2389 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3055 4820 4994 65
Size: 1183 1807 1765 174 1396
Fragments arranged by size:
1807 1765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2430 2809 4969 5156 1700
Size: 110 620 379 2160 187 2869
Fragments arranged by size:
2869 2160 620 379 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6325
BssSI C'ACGA_G
Cuts at: 4385 5397 4385
Size: 1012 5313
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6325
BstBI TT'CG_AA
Cuts at: 5129 5129
Size: 6325
BstEII G'GTnAC_C
Cuts at: 1184 2131 2890 2965 1184
Size: 947 759 75 4544
Fragments arranged by size:
4544 947 759 75
BstXI CCAn_nnnn'nTGG
Cuts at: 2887 2962 3341 2887
Size: 75 379 5871
Fragments arranged by size:
5871 379 75
BstYI r'GATC_y
Cuts at: 1972 2107 2788 3381 3655 4022 4407 4424 5876
Size: 135 681 593 274 367 385 17 1452
Cuts at: 5876 5962 5974 6320 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 681 593 385 367 346 274
135 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2860 2961 3231 513
Size: 552 1795 101 270 3607
Fragments arranged by size:
3607 1795 552 270 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5586
Fragments arranged by size:
5586 683 56
BtsI GCAGTG_nn'
Cuts at: 2421 2940 3841 4635 4655 2421
Size: 519 901 794 20 4091
Fragments arranged by size:
4091 901 794 519 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6290
DraI TTT'AAA
Cuts at: 3629 3629
Size: 6325
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5332 809
Size: 507 4016 1802
Fragments arranged by size:
4016 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3772 4661 6290
Size: 54 1425 50 275 18 1942 889 1629
Cuts at: 6290 8
Size: 43
Fragments arranged by size:
1942 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3772 1537
Size: 275 1960 4090
Fragments arranged by size:
4090 1960 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2282 4255 1353
Size: 929 1973 3423
Fragments arranged by size:
3423 1973 929
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4912 5286 5432 387
Size: 1064 138 3323 374 146 1280
Fragments arranged by size:
3323 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2009 2075 2087 2203 2308 2423 2897
Size: 393 377 66 12 116 105 115 474
Cuts at: 2897 3028 3579 4388 4985 5772 6040 1239
Size: 131 551 809 597 787 268 1524
Fragments arranged by size:
1524 809 787 597 551 474 393 377
268 131 116 115 105 66 12
EcoO109I rG'GnC_Cy
Cuts at: 2767 2767
Size: 6325
EcoRI G'AATT_C
Cuts at: 3634 4012 3634
Size: 378 5947
FspI TGC'GCA
Cuts at: 4831 4831
Size: 6325
HaeII r_GCGC'y
Cuts at: 1678 5472 1678
Size: 3794 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2778 2810 2901 2933 4966
Size: 32 135 32 1206 32 91 32 2033
Cuts at: 4966 4998 5040 5072 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2033 1206 135 91 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2632 3256 3669 3915 669
Size: 988 281 694 624 413 246 3079
Fragments arranged by size:
3079 988 694 624 413 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6325
HpaI GTT'AAC
Cuts at: 3915 3915
Size: 6325
KpnI G_GTAC'C
Cuts at: 3650 3650
Size: 6325
MfeI C'AATT_G
Cuts at: 3924 3924
Size: 6325
MluI A'CGCG_T
Cuts at: 6201 6201
Size: 6325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5439 5623 1305
Size: 4134 184 2007
Fragments arranged by size:
4134 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4503
Fragments arranged by size:
4503 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2129 2312 2393 2972 4283 4642 4801
Size: 648 963 183 81 579 1311 359 159
Cuts at: 4801 518
Size: 2042
Fragments arranged by size:
2042 1311 963 648 579 359 183 159
81
MspA1I CmG'CkG
Cuts at: 1606 3741 4418 5566 5811 1606
Size: 2135 677 1148 245 2120
Fragments arranged by size:
2135 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2811 2811
Size: 6325
NcoI C'CATG_G
Cuts at: 513 1065 3231 513
Size: 552 2166 3607
Fragments arranged by size:
3607 2166 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6325
NgoMIV G'CCGG_C
Cuts at: 2809 2809
Size: 6325
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6325
NotI GC'GGCC_GC
Cuts at: 3772 3772
Size: 6325
NsiI A_TGCA'T
Cuts at: 3552 3552
Size: 6325
NspI r_CATG'y
Cuts at: 1132 1229 3301 3683 5228 1132
Size: 97 2072 382 1545 2229
Fragments arranged by size:
2229 2072 1545 382 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6325
PciI A'CATG_T
Cuts at: 3297 5224 3297
Size: 1927 4398
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6325
PfoI T'CCnGG_A
Cuts at: 2091 2343 2091
Size: 252 6073
PmeI GTTT'AAAC
Cuts at: 3629 3629
Size: 6325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4394 4426 5907 5939 4394
Size: 32 1481 32 4780
Fragments arranged by size:
4780 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2767 2767
Size: 6325
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6325
PsiI TTA'TAA
Cuts at: 3895 3895
Size: 6325
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2816 2848 3021 3053 2816
Size: 32 173 32 6088
Fragments arranged by size:
6088 173 32 32
PstI C_TGCA'G
Cuts at: 830 2847 2902 3677 830
Size: 2017 55 775 3478
Fragments arranged by size:
3478 2017 775 55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6325
SalI G'TCGA_C
Cuts at: 1936 2630 3667 1936
Size: 694 1037 4594
Fragments arranged by size:
4594 1037 694
SanDI GG'GwC_CC
Cuts at: 2767 2767
Size: 6325
SbfI CC_TGCA'GG
Cuts at: 3677 3677
Size: 6325
ScaI AGT'ACT
Cuts at: 4573 6285 4573
Size: 1712 4613
SfcI C'TryA_G
Cuts at: 826 1045 2843 2898 3451 3673 4808 5489 5680
Size: 219 1798 55 553 222 1135 681 191
Cuts at: 5680 826
Size: 1471
Fragments arranged by size:
1798 1471 1135 681 553 222 219 191
55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6325
SmaI CCC'GGG
Cuts at: 1872 3652 1872
Size: 1780 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2703 4429 5330 5592 820
Size: 197 926 760 1726 901 262 1553
Fragments arranged by size:
1726 1553 926 901 760 262 197
SnaBI TAC'GTA
Cuts at: 493 4116 493
Size: 3623 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6325
SphI G_CATG'C
Cuts at: 1132 3683 1132
Size: 2551 3774
SspI AAT'ATT
Cuts at: 5 52 4249 5
Size: 47 4197 2081
Fragments arranged by size:
4197 2081 47
StuI AGG'CCT
Cuts at: 1517 3189 1517
Size: 1672 4653
StyI C'CwwG_G
Cuts at: 513 1065 2127 2535 2946 3024 3231 3764 513
Size: 552 1062 408 411 78 207 533 3074
Fragments arranged by size:
3074 1062 552 533 411 408 207 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4352 4369 4522 4707 5152 325
Size: 1255 2772 17 153 185 445 1498
Fragments arranged by size:
2772 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2138 4571 6283
Size: 274 80 33 51 1443 161 2433 1712
Cuts at: 6283 96
Size: 138
Fragments arranged by size:
2433 1712 1443 274 161 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6285 6317 6285
Size: 32 6293
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6325
XbaI T'CTAG_A
Cuts at: 3661 3661
Size: 6325
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6325
XmaI C'CCGG_G
Cuts at: 1870 3650 1870
Size: 1780 4545
XmnI GAAnn'nnTTC
Cuts at: 4454 4454
Size: 6325
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4133 276
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI PacI PmlI PspOMI PspXI PvuII RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI