Restriction map for
pFN21AE9415
(Circular) (Six-base) MAPSORT of: pFN21AE9415.seq Check: 875 from: 1 to: 5791
pFN21AE9415 5791 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3601 278
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2931 3134 1937
Size: 994 203 4594
Fragments arranged by size:
4594 994 203
Acc65I G'GTAC_C
Cuts at: 3112 3112
Size: 5791
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3854 5238 5506 1632
Size: 2222 1384 268 1917
Fragments arranged by size:
2222 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5594
AflIII A'CryG_T
Cuts at: 1203 4690 5667 1203
Size: 3487 977 1327
Fragments arranged by size:
3487 1327 977
AgeI A'CCGG_T
Cuts at: 4622 4622
Size: 5791
AhdI GACnn_n'nnGTC
Cuts at: 2424 4520 2424
Size: 2096 3695
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2675 2744 1184
Size: 1491 69 4231
Fragments arranged by size:
4231 1491 69
AlwNI CAG_nnn'CTG
Cuts at: 3534 5106 3534
Size: 1572 4219
ApaLI G'TGCA_C
Cuts at: 2558 3848 5004 2558
Size: 1290 1156 3345
Fragments arranged by size:
3345 1290 1156
ApoI r'AATT_y
Cuts at: 1493 3100 3326 3478 5587 1493
Size: 1607 226 152 2109 1697
Fragments arranged by size:
2109 1697 1607 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 3116 1870
Size: 73 1173 4545
Fragments arranged by size:
4545 1173 73
AvrII C'CTAG_G
Cuts at: 2593 2593
Size: 5791
BamHI G'GATC_C
Cuts at: 3121 3488 3121
Size: 367 5424
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2407 2763 2803 2870 3112
Size: 325 217 627 620 356 40 67 242
Cuts at: 3112 4567 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 620 356 325 242 217
67 40
BanII G_rGCy'C
Cuts at: 1391 3110 1391
Size: 1719 4072
BbeI G_GCGC'C
Cuts at: 2767 2807 2874 2767
Size: 40 67 5684
Fragments arranged by size:
5684 67 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2707 928
Size: 971 808 4012
Fragments arranged by size:
4012 971 808
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3982 4016 1903
Size: 34 2045 34 3678
Fragments arranged by size:
3678 2045 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3683 4893 3683
Size: 1210 4581
BclI T'GATC_A
Cuts at: 1548 2973 1548
Size: 1425 4366
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4402 136
Size: 107 122 71 631 605 2730 1525
Fragments arranged by size:
2730 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5786 5786
Size: 5791
BlpI GC'TnA_GC
Cuts at: 3208 3208
Size: 5791
Bme1580I G_kGCm'C
Cuts at: 1790 2412 2562 2864 3063 3852 5008 1790
Size: 622 150 302 199 789 1156 2573
Fragments arranged by size:
2573 1156 789 622 302 199 150
BmgBI CAC'GTC
Cuts at: 1318 2233 1318
Size: 915 4876
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3522 4480 442
Size: 416 53 83 399 24 2105 958 1753
Fragments arranged by size:
2105 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 2286 4451 1239
Size: 947 100 2165 2579
Fragments arranged by size:
2579 2165 947 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3916 4781 5079 3916
Size: 865 298 4628
Fragments arranged by size:
4628 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2166 3081 4454 882
Size: 617 667 915 1373 2219
Fragments arranged by size:
2219 1373 915 667 617
BsaAI yAC'GTr
Cuts at: 493 1204 2683 2740 3582 493
Size: 711 1479 57 842 2702
Fragments arranged by size:
2702 1479 842 711 57
BsaBI GATnn'nnATC
Cuts at: 5785 5785
Size: 5791
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2764 2804 2871 3598
Size: 53 83 186 936 1231 40 67 727
Cuts at: 3598 3980 4615 275
Size: 382 635 1451
Fragments arranged by size:
1451 1231 936 727 635 382 186 83
67 53 40
BsaWI w'CCGG_w
Cuts at: 4224 4622 4896 5043 5454 4224
Size: 398 274 147 411 4561
Fragments arranged by size:
4561 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2187 2217 1178
Size: 30 165 30 784 30 4752
Fragments arranged by size:
4752 784 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5791
BseYI C'CCAG_C
Cuts at: 2189 2617 2857 3080 4994 5490 2189
Size: 428 240 223 1914 496 2490
Fragments arranged by size:
2490 1914 496 428 240 223
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2714 1163
Size: 573 978 4240
Fragments arranged by size:
4240 978 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 3241 4002 5030 1540
Size: 275 186 57 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 275 186 57
BsiHKAI G_wGCw'C
Cuts at: 2238 2562 3852 3937 5008 2238
Size: 324 1290 85 1071 3021
Fragments arranged by size:
3021 1290 1071 324 85
BsmI GAATG_Cn'
Cuts at: 3302 3395 3302
Size: 93 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2238 2412 2562 2864 3063 3110 3852
Size: 399 448 174 150 302 199 47 742
Cuts at: 3852 3937 5008 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 448 399 302 199 174
150 85 47
BspEI T'CCGG_A
Cuts at: 5454 5454
Size: 5791
BspHI T'CATG_A
Cuts at: 3680 5410 3680
Size: 1730 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3146 844
Size: 2302 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2066 3678 1394
Size: 429 243 1612 3507
Fragments arranged by size:
3507 1612 429 243
BsrDI GCAATG_nn'
Cuts at: 65 1248 4286 4460 65
Size: 1183 3038 174 1396
Fragments arranged by size:
3038 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2141 4435 4622 1700
Size: 110 331 2294 187 2869
Fragments arranged by size:
2869 2294 331 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5791
BssHII G'CGCG_C
Cuts at: 1925 2641 2672 3016 1925
Size: 716 31 344 4700
Fragments arranged by size:
4700 716 344 31
BssSI C'ACGA_G
Cuts at: 2447 3851 4863 2447
Size: 1404 1012 3375
Fragments arranged by size:
3375 1404 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2566 794
Size: 1772 4019
BstBI TT'CG_AA
Cuts at: 4595 4595
Size: 5791
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5791
BstXI CCAn_nnnn'nTGG
Cuts at: 2494 2494
Size: 5791
BstYI r'GATC_y
Cuts at: 1972 3009 3121 3488 3873 3890 5342 5428 5440
Size: 1037 112 367 385 17 1452 86 12
Cuts at: 5440 5786 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1037 385 367 346 112 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2225 513
Size: 552 1160 4079
Fragments arranged by size:
4079 1160 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2278 487
Size: 683 56 1052 4000
Fragments arranged by size:
4000 1052 683 56
BtsI GCAGTG_nn'
Cuts at: 2794 3307 4101 4121 2794
Size: 513 794 20 4464
Fragments arranged by size:
4464 794 513 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5756
DraI TTT'AAA
Cuts at: 3095 3095
Size: 5791
DraIII CAC_nnn'GTG
Cuts at: 2392 2392
Size: 5791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2927 4798 809
Size: 507 1611 1871 1802
Fragments arranged by size:
1871 1802 1611 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2076 2346
Size: 54 1425 50 275 18 225 21 270
Cuts at: 2346 2484 3238 4127 5756 8
Size: 138 754 889 1629 43
Fragments arranged by size:
1629 1425 889 754 275 270 225 138
54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2055 3238 1537
Size: 275 243 1183 4090
Fragments arranged by size:
4090 1183 275 243
EarI CTCTTCn'nnn_
Cuts at: 1353 3721 1353
Size: 2368 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2163 2686 4378 4752 4898 387
Size: 1064 138 574 523 1692 374 146 1280
Fragments arranged by size:
1692 1280 1064 574 523 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2286 3854 4451 5238 5506 1239
Size: 393 554 100 1568 597 787 268 1524
Fragments arranged by size:
1568 1524 787 597 554 393 268 100
EcoO109I rG'GnC_Cy
Cuts at: 2114 2920 2114
Size: 806 4985
EcoRI G'AATT_C
Cuts at: 3100 3478 3100
Size: 378 5413
FspI TGC'GCA
Cuts at: 2315 2744 4297 2315
Size: 429 1553 3809
Fragments arranged by size:
3809 1553 429
FspAI rTGC'GCAy
Cuts at: 2744 2744
Size: 5791
HaeII r_GCGC'y
Cuts at: 1678 2019 2291 2312 2767 2807 2874 4938 1678
Size: 341 272 21 455 40 67 2064 2531
Fragments arranged by size:
2531 2064 455 341 272 67 40 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2410 2442 4432 4464 4506
Size: 32 135 32 838 32 1990 32 42
Cuts at: 4506 4538 1373
Size: 32 2626
Fragments arranged by size:
2626 1990 838 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3135 3381 669
Size: 988 281 1197 246 3079
Fragments arranged by size:
3079 1197 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5791
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KasI G'GCGC_C
Cuts at: 2763 2803 2870 2763
Size: 40 67 5684
Fragments arranged by size:
5684 67 40
KpnI G_GTAC'C
Cuts at: 3116 3116
Size: 5791
MfeI C'AATT_G
Cuts at: 3390 3390
Size: 5791
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4905 5089 1305
Size: 3600 184 2007
Fragments arranged by size:
3600 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2348 2486 10
Size: 54 1425 343 516 138 3315
Fragments arranged by size:
3315 1425 516 343 138 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 2327 2492 3749 4108 4267 518
Size: 648 1155 6 165 1257 359 159 2042
Fragments arranged by size:
2042 1257 1155 648 359 165 159 6
MspA1I CmG'CkG
Cuts at: 1606 2182 2216 2354 2668 2877 3207 3884 5032
Size: 576 34 138 314 209 330 677 1148
Cuts at: 5032 5277 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 576 330 314 245 209
138 34
NaeI GCC'GGC
Cuts at: 2143 2143
Size: 5791
NarI GG'CG_CC
Cuts at: 2764 2804 2871 2764
Size: 40 67 5684
Fragments arranged by size:
5684 67 40
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5239
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5791
NgoMIV G'CCGG_C
Cuts at: 2141 2141
Size: 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 3149 4694 1132
Size: 97 1920 1545 2229
Fragments arranged by size:
2229 1920 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5791
PciI A'CATG_T
Cuts at: 4690 4690
Size: 5791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2044 2404 1108
Size: 936 360 4495
Fragments arranged by size:
4495 936 360
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PmlI CAC'GTG
Cuts at: 2683 2683
Size: 5791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 5373 5405 3860
Size: 32 1481 32 4246
Fragments arranged by size:
4246 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2114 2920 2114
Size: 806 4985
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3361 3361
Size: 5791
PstI C_TGCA'G
Cuts at: 830 2654 2790 3143 830
Size: 1824 136 353 3478
Fragments arranged by size:
3478 1824 353 136
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
PvuII CAG'CTG
Cuts at: 2182 2877 2182
Size: 695 5096
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SanDI GG'GwC_CC
Cuts at: 2114 2114
Size: 5791
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 2402 4039 5751 2402
Size: 1637 1712 2442
Fragments arranged by size:
2442 1712 1637
SfcI C'TryA_G
Cuts at: 826 1045 2650 2786 3139 4274 4955 5146 826
Size: 219 1605 136 353 1135 681 191 1471
Fragments arranged by size:
1605 1471 1135 681 353 219 191 136
SfoI GGC'GCC
Cuts at: 2765 2805 2872 2765
Size: 40 67 5684
Fragments arranged by size:
5684 67 40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5791
SmaI CCC'GGG
Cuts at: 1872 3118 1872
Size: 1246 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3895 4796 5058 820
Size: 197 926 1952 901 262 1553
Fragments arranged by size:
1952 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5791
SphI G_CATG'C
Cuts at: 1132 3149 1132
Size: 2017 3774
SspI AAT'ATT
Cuts at: 5 52 3715 5
Size: 47 3663 2081
Fragments arranged by size:
3663 2081 47
StuI AGG'CCT
Cuts at: 1517 2598 1517
Size: 1081 4710
StyI C'CwwG_G
Cuts at: 513 1065 2593 3230 513
Size: 552 1528 637 3074
Fragments arranged by size:
3074 1528 637 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3818 3835 3988 4173 4618 325
Size: 1255 2238 17 153 185 445 1498
Fragments arranged by size:
2238 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2400 4037 5749
Size: 274 80 33 51 1443 423 1637 1712
Cuts at: 5749 96
Size: 138
Fragments arranged by size:
1712 1637 1443 423 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2187 2219 5751 5783 2187
Size: 32 3532 32 2195
Fragments arranged by size:
3532 2195 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2587 1316
Size: 1271 4520
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2329 2833 2899 2329
Size: 504 66 5221
Fragments arranged by size:
5221 504 66
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5791
XmaI C'CCGG_G
Cuts at: 1870 3116 1870
Size: 1246 4545
XmnI GAAnn'nnTTC
Cuts at: 3920 3920
Size: 5791
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3599 276
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI NruI NsiI PacI PfoI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SrfI
SwaI