Restriction map for
pFN21AE9414
(Circular) (Six-base) MAPSORT of: pFN21AE9414.seq Check: 4276 from: 1 to: 5788
pFN21AE9414 5788 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 1937
Size: 1194 4594
Acc65I G'GTAC_C
Cuts at: 3109 3109
Size: 5788
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3851 5235 5503 1632
Size: 2219 1384 268 1917
Fragments arranged by size:
2219 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 2106 1166
Size: 940 4848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 3531 5103 3531
Size: 1572 4216
ApaLI G'TGCA_C
Cuts at: 3010 3845 5001 3010
Size: 835 1156 3797
Fragments arranged by size:
3797 1156 835
ApoI r'AATT_y
Cuts at: 1493 2481 2688 3097 3323 3475 5584 1493
Size: 988 207 409 226 152 2109 1697
Fragments arranged by size:
2109 1697 988 409 226 207 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2874 3113 1870
Size: 73 931 239 4545
Fragments arranged by size:
4545 931 239 73
AvrII C'CTAG_G
Cuts at: 3059 3059
Size: 5788
BamHI G'GATC_C
Cuts at: 2034 3118 3485 2034
Size: 1084 367 4337
Fragments arranged by size:
4337 1084 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3109 4564 618
Size: 325 217 627 1322 1455 1842
Fragments arranged by size:
1842 1455 1322 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2731 3107 1391
Size: 1340 376 4072
Fragments arranged by size:
4072 1340 376
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2998 928
Size: 971 1099 3718
Fragments arranged by size:
3718 1099 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 2163 1548
Size: 615 5173
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4399 136
Size: 107 122 71 631 605 2727 1525
Fragments arranged by size:
2727 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5783 5783
Size: 5788
BlpI GC'TnA_GC
Cuts at: 3205 3205
Size: 5788
Bme1580I G_kGCm'C
Cuts at: 1790 3014 3849 5005 1790
Size: 1224 835 1156 2573
Fragments arranged by size:
2573 1224 1156 835
BmgBI CAC'GTC
Cuts at: 1318 2589 1318
Size: 1271 4517
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3519 4477 442
Size: 416 53 83 399 24 2102 958 1753
Fragments arranged by size:
2102 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4448 1239
Size: 3209 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2497 3913 4778 5076 2497
Size: 1416 865 298 3209
Fragments arranged by size:
3209 1416 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4451 882
Size: 617 2952 2219
Fragments arranged by size:
2952 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3579 493
Size: 711 2375 2702
Fragments arranged by size:
2702 2375 711
BsaBI GATnn'nnATC
Cuts at: 2421 2501 5782 2421
Size: 80 3281 2427
Fragments arranged by size:
3281 2427 80
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3595 3977 4612 275
Size: 53 83 186 936 2062 382 635 1451
Fragments arranged by size:
2062 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4221 4619 4893 5040 5451 4221
Size: 398 274 147 411 4558
Fragments arranged by size:
4558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2945 2975 1178
Size: 30 165 30 1542 30 3991
Fragments arranged by size:
3991 1542 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5788
BseYI C'CCAG_C
Cuts at: 2171 2541 4991 5487 2171
Size: 370 2450 496 2472
Fragments arranged by size:
2472 2450 496 370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5215
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 3999 5027 1540
Size: 275 186 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2209 3014 3849 3934 5005 2209
Size: 805 835 85 1071 2992
Fragments arranged by size:
2992 1071 835 805 85
BsmI GAATG_Cn'
Cuts at: 3299 3392 3299
Size: 93 5695
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2209 2731 3014 3107 3849 3934 5005
Size: 399 419 522 283 93 742 85 1071
Cuts at: 5005 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 522 419 399 283 93
85
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 2743 3677 5407 2743
Size: 934 1730 3124
Fragments arranged by size:
3124 1730 934
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3143 844
Size: 2299 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3675 1394
Size: 429 1852 3507
Fragments arranged by size:
3507 1852 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2166 4283 4457 65
Size: 1183 918 2117 174 1396
Fragments arranged by size:
2117 1396 1183 918 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4432 4619 1700
Size: 110 2622 187 2869
Fragments arranged by size:
2869 2622 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 2330 3848 4860 2330
Size: 1518 1012 3258
Fragments arranged by size:
3258 1518 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2399 794
Size: 1605 4183
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 2461 2555 1184
Size: 1277 94 4417
Fragments arranged by size:
4417 1277 94
BstXI CCAn_nnnn'nTGG
Cuts at: 2354 2354
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 2034 2502 3118 3485 3870 3887 5339 5425
Size: 62 468 616 367 385 17 1452 86
Cuts at: 5425 5437 5783 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 616 468 385 367 346 86
62 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2560 2796 513
Size: 552 1495 236 3505
Fragments arranged by size:
3505 1495 552 236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 2056 3304 4098 4118 2056
Size: 1248 794 20 3726
Fragments arranged by size:
3726 1248 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 3235 4124
Size: 54 1425 50 275 18 268 1137 889
Cuts at: 4124 5753 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1137 889 275 268 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2719 3718 1353
Size: 1366 999 3423
Fragments arranged by size:
3423 1366 999
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4375 4749 4895 387
Size: 1064 138 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3851 4448 5235 5503 1239
Size: 393 2219 597 787 268 1524
Fragments arranged by size:
2219 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 4935 1678
Size: 3257 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2349 2381 4429 4461 4503
Size: 32 135 32 777 32 2048 32 42
Cuts at: 4503 4535 1373
Size: 32 2626
Fragments arranged by size:
2626 2048 777 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KpnI G_GTAC'C
Cuts at: 3113 3113
Size: 5788
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2254 2915 2988 4902 5086 1305
Size: 949 661 73 1914 184 2007
Fragments arranged by size:
2007 1914 949 661 184 73
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2100 10
Size: 54 1425 343 268 3698
Fragments arranged by size:
3698 1425 343 268 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2106 2411 3746 4105 4264 518
Size: 648 940 305 1335 359 159 2042
Fragments arranged by size:
2042 1335 940 648 359 305 159
MspA1I CmG'CkG
Cuts at: 1606 3204 3881 5029 5274 1606
Size: 1598 677 1148 245 2120
Fragments arranged by size:
2120 1598 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2560 2796 513
Size: 552 1495 236 3505
Fragments arranged by size:
3505 1495 552 236
NdeI CA'TA_TG
Cuts at: 387 2257 387
Size: 1870 3918
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 2397 3146 4691 1132
Size: 97 1168 749 1545 2229
Fragments arranged by size:
2229 1545 1168 749 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5788
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2592 1108
Size: 1484 4304
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PstI C_TGCA'G
Cuts at: 830 3140 830
Size: 2310 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SapI GCTCTTCn'nnn_
Cuts at: 2719 2719
Size: 5788
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SexAI A'CCwGG_T
Cuts at: 2551 2551
Size: 5788
SfcI C'TryA_G
Cuts at: 826 1045 2104 3136 4271 4952 5143 826
Size: 219 1059 1032 1135 681 191 1471
Fragments arranged by size:
1471 1135 1059 1032 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 2876 3115 1872
Size: 1004 239 4545
Fragments arranged by size:
4545 1004 239
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2476 3892 4793 5055 820
Size: 197 926 533 1416 901 262 1553
Fragments arranged by size:
1553 1416 926 901 533 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 2985 1517
Size: 1468 4320
StyI C'CwwG_G
Cuts at: 513 1065 2560 2796 3059 3227 513
Size: 552 1495 236 263 168 3074
Fragments arranged by size:
3074 1495 552 263 236 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2736 3815 3832 3985 4170 4615 325
Size: 1255 1156 1079 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1156 1079 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4034 5746 96
Size: 274 80 33 51 1443 2057 1712 138
Fragments arranged by size:
2057 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2803 2835 5748 5780 2803
Size: 32 2913 32 2811
Fragments arranged by size:
2913 2811 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 2863 3124 2863
Size: 261 5527
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 2874 3113 1870
Size: 1004 239 4545
Fragments arranged by size:
4545 1004 239
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI XcmI