Restriction map for
pFN21AE9411
(Circular) (Six-base) MAPSORT of: pFN21AE9411.seq Check: 7247 from: 1 to: 5773
pFN21AE9411 5773 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2714 2714
Size: 5773
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3583 278
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3116 1937
Size: 1179 4594
Acc65I G'GTAC_C
Cuts at: 3094 3094
Size: 5773
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3836 5220 5488 1632
Size: 2204 1384 268 1917
Fragments arranged by size:
2204 1917 1384 268
AfeI AGC'GCT
Cuts at: 2794 2794
Size: 5773
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5576
AflIII A'CryG_T
Cuts at: 1203 2063 4672 5649 1203
Size: 860 2609 977 1327
Fragments arranged by size:
2609 1327 977 860
AgeI A'CCGG_T
Cuts at: 4604 4604
Size: 5773
AhdI GACnn_n'nnGTC
Cuts at: 4502 4502
Size: 5773
AleI CACnn'nnGTG
Cuts at: 1166 2363 1166
Size: 1197 4576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5773
AlwNI CAG_nnn'CTG
Cuts at: 2034 2391 3516 5088 2034
Size: 357 1125 1572 2719
Fragments arranged by size:
2719 1572 1125 357
ApaI G_GGCC'C
Cuts at: 2452 2688 2452
Size: 236 5537
ApaLI G'TGCA_C
Cuts at: 3830 4986 3830
Size: 1156 4617
ApoI r'AATT_y
Cuts at: 1493 2537 3082 3308 3460 5569 1493
Size: 1044 545 226 152 2109 1697
Fragments arranged by size:
2109 1697 1044 545 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4327 160
Size: 872 3295 1606
Fragments arranged by size:
3295 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 3066 3098 1870
Size: 73 1123 32 4545
Fragments arranged by size:
4545 1123 73 32
BamHI G'GATC_C
Cuts at: 3103 3470 3103
Size: 367 5406
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2702 3094 4549 618
Size: 325 217 627 915 392 1455 1842
Fragments arranged by size:
1842 1455 915 627 392 325 217
BanII G_rGCy'C
Cuts at: 1391 2452 2688 3092 1391
Size: 1061 236 404 4072
Fragments arranged by size:
4072 1061 404 236
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2203 2395 2947 928
Size: 971 304 192 552 3754
Fragments arranged by size:
3754 971 552 304 192
BbvCI CC'TCA_GC
Cuts at: 2372 2372
Size: 5773
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3964 3998 1903
Size: 34 2027 34 3678
Fragments arranged by size:
3678 2027 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4875 3665
Size: 1210 4563
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5773
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2770 4384 136
Size: 107 122 71 631 605 1098 1614 1525
Fragments arranged by size:
1614 1525 1098 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5768 5768
Size: 5773
BlpI GC'TnA_GC
Cuts at: 3190 3190
Size: 5773
Bme1580I G_kGCm'C
Cuts at: 1790 2452 2688 3834 4990 1790
Size: 662 236 1146 1156 2573
Fragments arranged by size:
2573 1156 1146 662 236
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5773
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3504 4462 442
Size: 416 53 83 399 24 2087 958 1753
Fragments arranged by size:
2087 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2907 1056
Size: 1851 3922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2299 2882 4433 1239
Size: 1060 583 1551 2579
Fragments arranged by size:
2579 1551 1060 583
Bpu10I CC'TnA_GC
Cuts at: 2372 2689 2372
Size: 317 5456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2846 3898 4763 5061 2846
Size: 1052 865 298 3558
Fragments arranged by size:
3558 1052 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2020 2236 4436 882
Size: 617 521 216 2200 2219
Fragments arranged by size:
2219 2200 617 521 216
BsaAI yAC'GTr
Cuts at: 493 1204 2066 3564 493
Size: 711 862 1498 2702
Fragments arranged by size:
2702 1498 862 711
BsaBI GATnn'nnATC
Cuts at: 5767 5767
Size: 5773
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3580 3962 4597 275
Size: 53 83 186 936 2047 382 635 1451
Fragments arranged by size:
2047 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2099 2541 4206 4604 4878 5025 5436 2099
Size: 442 1665 398 274 147 411 2436
Fragments arranged by size:
2436 1665 442 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2108 2138 3023 3053 1178
Size: 30 165 30 705 30 885 30 3898
Fragments arranged by size:
3898 885 705 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2318 3041 3044 1186
Size: 943 189 723 3 3915
Fragments arranged by size:
3915 943 723 189 3
BseYI C'CCAG_C
Cuts at: 2179 2671 2745 4976 5472 2179
Size: 492 74 2231 496 2480
Fragments arranged by size:
2480 2231 496 492 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2489 1163
Size: 573 753 4447
Fragments arranged by size:
4447 753 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3223 3984 5012 1540
Size: 275 186 1222 761 1028 2301
Fragments arranged by size:
2301 1222 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2525 3834 3919 4990 2525
Size: 1309 85 1071 3308
Fragments arranged by size:
3308 1309 1071 85
BsmI GAATG_Cn'
Cuts at: 3284 3377 3284
Size: 93 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2452 2525 2688 3092 3834 3919 4990
Size: 399 662 73 163 404 742 85 1071
Cuts at: 4990 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 662 404 399 163 85
73
BspEI T'CCGG_A
Cuts at: 2099 2541 5436 2099
Size: 442 2895 2436
Fragments arranged by size:
2895 2436 442
BspHI T'CATG_A
Cuts at: 3662 5392 3662
Size: 1730 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2714 3128 844
Size: 1870 414 3489
Fragments arranged by size:
3489 1870 414
BsrBI CCG'CTC
Cuts at: 1394 1823 3660 1394
Size: 429 1837 3507
Fragments arranged by size:
3507 1837 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2191 2292 4268 4442 65
Size: 1183 943 101 1976 174 1396
Fragments arranged by size:
1976 1396 1183 943 174 101
BsrFI r'CCGG_y
Cuts at: 1700 1810 2760 4417 4604 1700
Size: 110 950 1657 187 2869
Fragments arranged by size:
2869 1657 950 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5773
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5773
BssSI C'ACGA_G
Cuts at: 3833 4845 3833
Size: 1012 4761
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5773
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5773
BstYI r'GATC_y
Cuts at: 1972 3103 3470 3855 3872 5324 5410 5422 5768
Size: 1131 367 385 17 1452 86 12 346
Cuts at: 5768 1972
Size: 1977
Fragments arranged by size:
1977 1452 1131 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3052 513
Size: 552 1987 3234
Fragments arranged by size:
3234 1987 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5034
Fragments arranged by size:
5034 683 56
BtsI GCAGTG_nn'
Cuts at: 2044 2327 2572 3289 4083 4103 2044
Size: 283 245 717 794 20 3714
Fragments arranged by size:
3714 794 717 283 245 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5738
DraI TTT'AAA
Cuts at: 3077 3077
Size: 5773
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4780 809
Size: 507 3464 1802
Fragments arranged by size:
3464 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2508 2659 3220
Size: 54 1425 50 275 18 678 151 561
Cuts at: 3220 4109 5738 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 678 561 275 151 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3220 1537
Size: 275 1408 4090
Fragments arranged by size:
4090 1408 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2101 3703 1353
Size: 748 1602 3423
Fragments arranged by size:
3423 1602 748
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2120 4360 4734 4880 387
Size: 1064 138 531 2240 374 146 1280
Fragments arranged by size:
2240 1280 1064 531 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2299 2882 3836 4433 5220 5488 1239
Size: 393 667 583 954 597 787 268 1524
Fragments arranged by size:
1524 954 787 667 597 583 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2111 2111
Size: 5773
EcoO109I rG'GnC_Cy
Cuts at: 2056 2124 2449 2684 2685 2056
Size: 68 325 235 1 5144
Fragments arranged by size:
5144 325 235 68 1
EcoRI G'AATT_C
Cuts at: 3082 3460 3082
Size: 378 5395
FspI TGC'GCA
Cuts at: 2529 4279 2529
Size: 1750 4023
HaeII r_GCGC'y
Cuts at: 1678 2796 4920 1678
Size: 1118 2124 2531
Fragments arranged by size:
2531 2124 1118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2053 2085 2108 2140 3023
Size: 32 135 32 481 32 23 32 883
Cuts at: 3023 3055 4414 4446 4488 4520 1373
Size: 32 1359 32 42 32 2626
Fragments arranged by size:
2626 1359 883 481 135 42 32 32
32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 2926 3117 3363 669
Size: 988 281 988 191 246 3079
Fragments arranged by size:
3079 988 988 281 246 191
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5773
HpaI GTT'AAC
Cuts at: 3363 3363
Size: 5773
KpnI G_GTAC'C
Cuts at: 3098 3098
Size: 5773
MfeI C'AATT_G
Cuts at: 3372 3372
Size: 5773
MluI A'CGCG_T
Cuts at: 5649 5649
Size: 5773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2152 4887 5071 1305
Size: 847 2735 184 2007
Fragments arranged by size:
2735 2007 847 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2661 10
Size: 54 1425 343 829 3122
Fragments arranged by size:
3122 1425 829 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 2363 2411 3731 4090 4249 518
Size: 648 1131 66 48 1320 359 159 2042
Fragments arranged by size:
2042 1320 1131 648 359 159 66 48
MspA1I CmG'CkG
Cuts at: 1606 2473 3189 3866 5014 5259 1606
Size: 867 716 677 1148 245 2120
Fragments arranged by size:
2120 1148 867 716 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5221
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5773
NheI G'CTAG_C
Cuts at: 1052 2903 1052
Size: 1851 3922
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 3131 4676 1132
Size: 97 1902 1545 2229
Fragments arranged by size:
2229 1902 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5773
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2322 2556 1108
Size: 1214 234 4325
Fragments arranged by size:
4325 1214 234
PfoI T'CCnGG_A
Cuts at: 2081 2081
Size: 5773
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PmlI CAC'GTG
Cuts at: 2066 2066
Size: 5773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2124 2056
Size: 68 5705
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5773
PsiI TTA'TAA
Cuts at: 3343 3343
Size: 5773
PspOMI G'GGCC_C
Cuts at: 2448 2684 2448
Size: 236 5537
PstI C_TGCA'G
Cuts at: 830 2039 3125 830
Size: 1209 1086 3478
Fragments arranged by size:
3478 1209 1086
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
SalI G'TCGA_C
Cuts at: 1936 3115 1936
Size: 1179 4594
SanDI GG'GwC_CC
Cuts at: 2124 2124
Size: 5773
SbfI CC_TGCA'GG
Cuts at: 3125 3125
Size: 5773
ScaI AGT'ACT
Cuts at: 4021 5733 4021
Size: 1712 4061
SfcI C'TryA_G
Cuts at: 826 1045 2035 3121 4256 4937 5128 826
Size: 219 990 1086 1135 681 191 1471
Fragments arranged by size:
1471 1135 1086 990 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 3068 3100 1872
Size: 1196 32 4545
Fragments arranged by size:
4545 1196 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2861 3877 4778 5040 820
Size: 197 926 918 1016 901 262 1553
Fragments arranged by size:
1553 1016 926 918 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 3131 1132
Size: 1999 3774
SspI AAT'ATT
Cuts at: 5 52 3697 5
Size: 47 3645 2081
Fragments arranged by size:
3645 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5773
StyI C'CwwG_G
Cuts at: 513 1065 2913 3212 513
Size: 552 1848 299 3074
Fragments arranged by size:
3074 1848 552 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3800 3817 3970 4155 4600 325
Size: 1255 2220 17 153 185 445 1498
Fragments arranged by size:
2220 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2301 4019 5731
Size: 274 80 33 51 1443 324 1718 1712
Cuts at: 5731 96
Size: 138
Fragments arranged by size:
1718 1712 1443 324 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5733 5765 5733
Size: 32 5741
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5773
XbaI T'CTAG_A
Cuts at: 3109 3109
Size: 5773
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5773
XmaI C'CCGG_G
Cuts at: 1870 3066 3098 1870
Size: 1196 32 4545
Fragments arranged by size:
4545 1196 32
XmnI GAAnn'nnTTC
Cuts at: 3902 3902
Size: 5773
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3581 276
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspXI PsrI PvuII RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI