Restriction map for
pFN21AE9410
(Circular) (Six-base) MAPSORT of: pFN21AE9410.seq Check: 4715 from: 1 to: 5662
pFN21AE9410 5662 bp
With 182 enzymes: SgfI *
July 27, 2011 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2450 2450
Size: 5662
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3472 278
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2157 2847 3005 1937
Size: 220 690 158 4594
Fragments arranged by size:
4594 690 220 158
Acc65I G'GTAC_C
Cuts at: 2340 2983 2340
Size: 643 5019
AclI AA'CG_TT
Cuts at: 1563 3789 4162 1563
Size: 2226 373 3063
Fragments arranged by size:
3063 2226 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2506 2540 2639 2854 3725 5109 5377 1632
Size: 874 34 99 215 871 1384 268 1917
Fragments arranged by size:
1917 1384 874 871 268 215 99 34
AflII C'TTAA_G
Cuts at: 820 1017 2366 820
Size: 197 1349 4116
Fragments arranged by size:
4116 1349 197
AflIII A'CryG_T
Cuts at: 1203 4561 5538 1203
Size: 3358 977 1327
Fragments arranged by size:
3358 1327 977
AgeI A'CCGG_T
Cuts at: 2337 4493 2337
Size: 2156 3506
AhdI GACnn_n'nnGTC
Cuts at: 4391 4391
Size: 5662
AleI CACnn'nnGTG
Cuts at: 1166 2039 2051 2681 1166
Size: 873 12 630 4147
Fragments arranged by size:
4147 873 630 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2566 1184
Size: 1382 4280
AlwNI CAG_nnn'CTG
Cuts at: 2671 2755 3405 4977 2671
Size: 84 650 1572 3356
Fragments arranged by size:
3356 1572 650 84
ApaI G_GGCC'C
Cuts at: 2272 2665 2773 2272
Size: 393 108 5161
Fragments arranged by size:
5161 393 108
ApaLI G'TGCA_C
Cuts at: 3719 4875 3719
Size: 1156 4506
ApoI r'AATT_y
Cuts at: 1493 2971 3197 3349 5458 1493
Size: 1478 226 152 2109 1697
Fragments arranged by size:
2109 1697 1478 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4216 160
Size: 872 3184 1606
Fragments arranged by size:
3184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2407 2446 2987 1870
Size: 73 464 39 541 4545
Fragments arranged by size:
4545 541 464 73 39
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2332 2365 2332
Size: 33 5629
BamHI G'GATC_C
Cuts at: 2992 3359 2992
Size: 367 5295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2340 2487 2983 4438 618
Size: 325 217 627 553 147 496 1455 1842
Fragments arranged by size:
1842 1455 627 553 496 325 217 147
BanII G_rGCy'C
Cuts at: 1391 2272 2614 2665 2773 2879 2931 2981 1391
Size: 881 342 51 108 106 52 50 4072
Fragments arranged by size:
4072 881 342 108 106 52 51 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2884 928
Size: 971 985 3706
Fragments arranged by size:
3706 985 971
BbvCI CC'TCA_GC
Cuts at: 2291 2291
Size: 5662
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3853 3887 1903
Size: 34 50 34 1832 34 3678
Fragments arranged by size:
3678 1832 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3554 4764 3554
Size: 1210 4452
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5662
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2259 2318 2327
Size: 107 122 71 631 605 587 59 9
Cuts at: 2327 4273 136
Size: 1946 1525
Fragments arranged by size:
1946 1525 631 605 587 122 107 71
59 9
BglII A'GATC_T
Cuts at: 5657 5657
Size: 5662
BlpI GC'TnA_GC
Cuts at: 2023 3079 2023
Size: 1056 4606
Bme1580I G_kGCm'C
Cuts at: 1790 2272 2665 2773 3723 4879 1790
Size: 482 393 108 950 1156 2573
Fragments arranged by size:
2573 1156 950 482 393 108
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3393 4351 442
Size: 416 53 83 399 24 1976 958 1753
Fragments arranged by size:
1976 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2219 2488 2600 2842 4322 1239
Size: 854 126 269 112 242 1480 2579
Fragments arranged by size:
2579 1480 854 269 242 126 112
Bpu10I CC'TnA_GC
Cuts at: 2228 2291 2228
Size: 63 5599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3787 4652 4950 3787
Size: 865 298 4499
Fragments arranged by size:
4499 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2410 4325 882
Size: 617 911 1915 2219
Fragments arranged by size:
2219 1915 911 617
BsaAI yAC'GTr
Cuts at: 493 1204 3453 493
Size: 711 2249 2702
Fragments arranged by size:
2702 2249 711
BsaBI GATnn'nnATC
Cuts at: 2741 5656 2741
Size: 2915 2747
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3469 3851 4486 275
Size: 53 83 186 936 1936 382 635 1451
Fragments arranged by size:
1936 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2337 4095 4493 4767 4914 5325 2337
Size: 1758 398 274 147 411 2674
Fragments arranged by size:
2674 1758 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2840 2870 1178
Size: 30 165 30 1437 30 3970
Fragments arranged by size:
3970 1437 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2376 1186
Size: 1190 4472
BseYI C'CCAG_C
Cuts at: 2030 2065 2083 2092 2265 4865 5361 2030
Size: 35 18 9 173 2600 496 2331
Fragments arranged by size:
2600 2331 496 173 35 18 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2799 2807 1163
Size: 573 1063 8 4018
Fragments arranged by size:
4018 1063 573 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3873 4901 1540
Size: 275 186 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2835 2879 3723 3808 4879 2835
Size: 44 844 85 1071 3618
Fragments arranged by size:
3618 1071 844 85 44
BsiWI C'GTAC_G
Cuts at: 2148 2148
Size: 5662
BsmI GAATG_Cn'
Cuts at: 3173 3266 3173
Size: 93 5569
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2272 2614 2665 2773 2835 2879 2931
Size: 399 482 342 51 108 62 44 52
Cuts at: 2931 2981 3723 3808 4879 1391
Size: 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 482 399 342 108 85
62 52 51 50 44
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 3551 5281 3551
Size: 1730 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 2193 2385 2450 2829 3017 844
Size: 1325 24 192 65 379 188 3489
Fragments arranged by size:
3489 1325 379 192 188 65 24
BsrBI CCG'CTC
Cuts at: 1394 1823 3549 1394
Size: 429 1726 3507
Fragments arranged by size:
3507 1726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2599 4157 4331 65
Size: 1183 1351 1558 174 1396
Fragments arranged by size:
1558 1396 1351 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2337 4306 4493 1700
Size: 110 527 1969 187 2869
Fragments arranged by size:
2869 1969 527 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5662
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 3722 4734 3722
Size: 1012 4650
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2830 794
Size: 2036 3626
BstBI TT'CG_AA
Cuts at: 4466 4466
Size: 5662
BstEII G'GTnAC_C
Cuts at: 1184 2674 1184
Size: 1490 4172
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 2053 2038
Size: 15 5647
BstYI r'GATC_y
Cuts at: 1972 2992 3359 3744 3761 5213 5299 5311 5657
Size: 1020 367 385 17 1452 86 12 346
Cuts at: 5657 1972
Size: 1977
Fragments arranged by size:
1977 1452 1020 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2848 2848
Size: 5662
Bsu36I CC'TnA_GG
Cuts at: 2388 2388
Size: 5662
BtgI C'CryG_G
Cuts at: 513 1065 2325 2841 513
Size: 552 1260 516 3334
Fragments arranged by size:
3334 1260 552 516
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4923
Fragments arranged by size:
4923 683 56
BtsI GCAGTG_nn'
Cuts at: 2787 3178 3972 3992 2787
Size: 391 794 20 4457
Fragments arranged by size:
4457 794 391 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5627
DraI TTT'AAA
Cuts at: 2966 2966
Size: 5662
DraIII CAC_nnn'GTG
Cuts at: 2487 2830 2487
Size: 343 5319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4669 809
Size: 507 3353 1802
Fragments arranged by size:
3353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2214 2319
Size: 54 1425 50 275 18 213 171 105
Cuts at: 2319 2724 3109 3998 5627 8
Size: 405 385 889 1629 43
Fragments arranged by size:
1629 1425 889 405 385 275 213 171
105 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 1537
Size: 275 1297 4090
Fragments arranged by size:
4090 1297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3592 1353
Size: 2239 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4249 4623 4769 387
Size: 1064 138 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2877 2877
Size: 5662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2219 2488 2506 2540 2600 2639
Size: 393 461 126 269 18 34 60 39
Cuts at: 2639 2842 2854 3725 4322 5109 5377 1239
Size: 203 12 871 597 787 268 1524
Fragments arranged by size:
1524 871 787 597 461 393 269 268
203 126 60 39 34 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2884 2884
Size: 5662
EcoO109I rG'GnC_Cy
Cuts at: 2269 2769 2770 2269
Size: 500 1 5161
Fragments arranged by size:
5161 500 1
EcoRI G'AATT_C
Cuts at: 2971 3349 2971
Size: 378 5284
FspI TGC'GCA
Cuts at: 4168 4168
Size: 5662
HaeII r_GCGC'y
Cuts at: 1678 2111 4809 1678
Size: 433 2698 2531
Fragments arranged by size:
2698 2531 433
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2866 2898 4303 4335 4377
Size: 32 135 32 1294 32 1405 32 42
Cuts at: 4377 4409 1373
Size: 32 2626
Fragments arranged by size:
2626 1405 1294 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2374 3006 3252 669
Size: 988 281 436 632 246 3079
Fragments arranged by size:
3079 988 632 436 281 246
HindIII A'AGCT_T
Cuts at: 748 2651 748
Size: 1903 3759
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KpnI G_GTAC'C
Cuts at: 2344 2987 2344
Size: 643 5019
MfeI C'AATT_G
Cuts at: 3261 3261
Size: 5662
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4776 4960 1305
Size: 3471 184 2007
Fragments arranged by size:
3471 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 2726 10
Size: 54 1425 343 213 681 2946
Fragments arranged by size:
2946 1425 681 343 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2051 2681 3620 3979 4138 518
Size: 648 873 12 630 939 359 159 2042
Fragments arranged by size:
2042 939 873 648 630 359 159 12
MspA1I CmG'CkG
Cuts at: 1606 2051 2659 3078 3755 4903 5148 1606
Size: 445 608 419 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 608 445 419 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5110
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5662
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5662
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 3020 4565 1132
Size: 97 1791 1545 2229
Fragments arranged by size:
2229 1791 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2398 1348
Size: 1050 4612
PciI A'CATG_T
Cuts at: 4561 4561
Size: 5662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2671 2755 1108
Size: 1563 84 4015
Fragments arranged by size:
4015 1563 84
PfoI T'CCnGG_A
Cuts at: 2628 2628
Size: 5662
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 5244 5276 3731
Size: 32 1481 32 4117
Fragments arranged by size:
4117 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5662
PsiI TTA'TAA
Cuts at: 3232 3232
Size: 5662
PspOMI G'GGCC_C
Cuts at: 2268 2661 2769 2268
Size: 393 108 5161
Fragments arranged by size:
5161 393 108
PstI C_TGCA'G
Cuts at: 830 2768 3014 830
Size: 1938 246 3478
Fragments arranged by size:
3478 1938 246
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
PvuII CAG'CTG
Cuts at: 2051 2051
Size: 5662
SacI G_AGCT'C
Cuts at: 2879 2879
Size: 5662
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SbfI CC_TGCA'GG
Cuts at: 3014 3014
Size: 5662
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SexAI A'CCwGG_T
Cuts at: 2483 2483
Size: 5662
SfcI C'TryA_G
Cuts at: 826 1045 2764 3010 4145 4826 5017 826
Size: 219 1719 246 1135 681 191 1471
Fragments arranged by size:
1719 1471 1135 681 246 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2318 2318
Size: 5662
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2448 2989 1872
Size: 576 541 4545
Fragments arranged by size:
4545 576 541
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2366 3766 4667 4929 820
Size: 197 926 423 1400 901 262 1553
Fragments arranged by size:
1553 1400 926 901 423 262 197
SnaBI TAC'GTA
Cuts at: 493 3453 493
Size: 2960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 3020 1132
Size: 1888 3774
SrfI GCCC'GGGC
Cuts at: 2448 2448
Size: 5662
SspI AAT'ATT
Cuts at: 5 52 3586 5
Size: 47 3534 2081
Fragments arranged by size:
3534 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5662
StyI C'CwwG_G
Cuts at: 513 1065 2679 3101 513
Size: 552 1614 422 3074
Fragments arranged by size:
3074 1614 552 422
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 3689 3706 3859 4044 4489 325
Size: 1255 465 1644 17 153 185 445 1498
Fragments arranged by size:
1644 1498 1255 465 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3908 5620 96
Size: 274 80 33 51 1443 1931 1712 138
Fragments arranged by size:
1931 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5622 5654 5622
Size: 32 5630
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5662
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2038
Size: 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5662
XmaI C'CCGG_G
Cuts at: 1870 2446 2987 1870
Size: 576 541 4545
Fragments arranged by size:
4545 576 541
XmnI GAAnn'nnTTC
Cuts at: 3791 3791
Size: 5662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3470 276
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsmBI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SapI SfoI SwaI