Restriction map for
pFN21AE9409
(Circular) (Six-base) MAPSORT of: pFN21AE9409.seq Check: 6855 from: 1 to: 5788
pFN21AE9409 5788 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2891 2891
Size: 5788
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2124 2532 3131 1937
Size: 187 408 599 4594
Fragments arranged by size:
4594 599 408 187
Acc65I G'GTAC_C
Cuts at: 3109 3109
Size: 5788
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2767 3851 5235 5503 1632
Size: 1135 1084 1384 268 1917
Fragments arranged by size:
1917 1384 1135 1084 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 2762 1166
Size: 1596 4192
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 2551 3531 5103 2551
Size: 980 1572 3236
Fragments arranged by size:
3236 1572 980
ApaI G_GGCC'C
Cuts at: 2213 2719 3058 2213
Size: 506 339 4943
Fragments arranged by size:
4943 506 339
ApaLI G'TGCA_C
Cuts at: 3845 5001 3845
Size: 1156 4632
ApoI r'AATT_y
Cuts at: 1493 3097 3323 3475 5584 1493
Size: 1604 226 152 2109 1697
Fragments arranged by size:
2109 1697 1604 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 2059 2438 2952 3113 1870
Size: 73 92 24 379 514 161 4545
Fragments arranged by size:
4545 514 379 161 92 73 24
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2220 2253 2220
Size: 33 5755
BamHI G'GATC_C
Cuts at: 3118 3485 3118
Size: 367 5421
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3109 4564 618
Size: 325 217 627 1322 1455 1842
Fragments arranged by size:
1842 1455 1322 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2022 2030 2213 2589 2719 2771 2953 3058
Size: 631 8 183 376 130 52 182 105
Cuts at: 3058 3107 1391
Size: 49 4072
Fragments arranged by size:
4072 631 376 183 182 130 105 52
49 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2794 928
Size: 971 895 3922
Fragments arranged by size:
3922 971 895
BbvCI CC'TCA_GC
Cuts at: 2609 2609
Size: 5788
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2434 2468 2693 2725 2727 2759 3979
Size: 34 497 34 225 32 2 32 1220
Cuts at: 3979 4013 1903
Size: 34 3678
Fragments arranged by size:
3678 1220 497 225 34 34 34 32
32 2
BciVI GTATCCnnnnn_n'
Cuts at: 2221 3680 4890 2221
Size: 1459 1210 3119
Fragments arranged by size:
3119 1459 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2169 2297 2577
Size: 107 122 71 631 605 497 128 280
Cuts at: 2577 2795 4399 136
Size: 218 1604 1525
Fragments arranged by size:
1604 1525 631 605 497 280 218 128
122 107 71
BglII A'GATC_T
Cuts at: 5783 5783
Size: 5788
BlpI GC'TnA_GC
Cuts at: 2345 3007 3205 2345
Size: 662 198 4928
Fragments arranged by size:
4928 662 198
Bme1580I G_kGCm'C
Cuts at: 1790 2213 2369 2719 2843 3058 3849 5005 1790
Size: 423 156 350 124 215 791 1156 2573
Fragments arranged by size:
2573 1156 791 423 350 215 156 124
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3519 4477 442
Size: 416 53 83 399 24 2102 958 1753
Fragments arranged by size:
2102 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2044 2096 2105 3007 4448 1239
Size: 805 52 9 902 1441 2579
Fragments arranged by size:
2579 1441 902 805 52 9
Bpu10I CC'TnA_GC
Cuts at: 2609 2609
Size: 5788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3913 4778 5076 3913
Size: 865 298 4625
Fragments arranged by size:
4625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4451 882
Size: 617 2952 2219
Fragments arranged by size:
2952 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2707 3579 493
Size: 711 1503 872 2702
Fragments arranged by size:
2702 1503 872 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2490 3595 3977 4612
Size: 53 83 186 936 957 1105 382 635
Cuts at: 4612 275
Size: 1451
Fragments arranged by size:
1451 1105 957 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4221 4619 4893 5040 5451 4221
Size: 398 274 147 411 4558
Fragments arranged by size:
4558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5563
Fragments arranged by size:
5563 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2597 3004 1186
Size: 907 504 407 3970
Fragments arranged by size:
3970 907 504 407
BseYI C'CCAG_C
Cuts at: 2030 2167 2862 4991 5487 2030
Size: 137 695 2129 496 2331
Fragments arranged by size:
2331 2129 695 496 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2225 1163
Size: 573 489 4726
Fragments arranged by size:
4726 573 489
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2100 2314 2699 2986 3238 3999
Size: 275 186 99 214 385 287 252 761
Cuts at: 3999 5027 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 385 287 275 252 214
186 99
BsiHKAI G_wGCw'C
Cuts at: 2274 2759 3849 3934 5005 2274
Size: 485 1090 85 1071 3057
Fragments arranged by size:
3057 1090 1071 485 85
BsmI GAATG_Cn'
Cuts at: 3299 3392 3299
Size: 93 5695
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2030 2213 2274 2369 2589 2719
Size: 399 232 8 183 61 95 220 130
Cuts at: 2719 2759 2771 2843 2953 3058 3107 3849 3934
Size: 40 12 72 110 105 49 742 85
Cuts at: 3934 5005 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 232 220 183 130
110 105 95 85 72 61 49 40
12 8
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2115 2891 3143 844
Size: 1271 776 252 3489
Fragments arranged by size:
3489 1271 776 252
BsrBI CCG'CTC
Cuts at: 1394 1823 2917 2940 3675 1394
Size: 429 1094 23 735 3507
Fragments arranged by size:
3507 1094 735 429 23
BsrDI GCAATG_nn'
Cuts at: 65 1248 2874 4283 4457 65
Size: 1183 1626 1409 174 1396
Fragments arranged by size:
1626 1409 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2513 2981 4432 4619 1700
Size: 110 703 468 1451 187 2869
Fragments arranged by size:
2869 1451 703 468 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 2382 2469 1925
Size: 457 87 5244
Fragments arranged by size:
5244 457 87
BssSI C'ACGA_G
Cuts at: 2479 3848 4860 2479
Size: 1369 1012 3407
Fragments arranged by size:
3407 1369 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2373 2551 794
Size: 1579 178 4031
Fragments arranged by size:
4031 1579 178
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstXI CCAn_nnnn'nTGG
Cuts at: 2887 2887
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 3118 3485 3870 3887 5339 5425 5437 5783
Size: 1146 367 385 17 1452 86 12 346
Cuts at: 5783 1972
Size: 1977
Fragments arranged by size:
1977 1452 1146 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2157 2213 2292 2424 2499 513
Size: 552 939 153 56 79 132 75 3802
Fragments arranged by size:
3802 939 552 153 132 79 75 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2343 487
Size: 683 56 1117 3932
Fragments arranged by size:
3932 1117 683 56
BtsI GCAGTG_nn'
Cuts at: 2450 3069 3304 4098 4118 2450
Size: 619 235 794 20 4120
Fragments arranged by size:
4120 794 619 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2196 2311
Size: 54 1425 50 275 18 267 99 115
Cuts at: 2311 2318 2421 2511 2696 2983 3235 4124 5753
Size: 7 103 90 185 287 252 889 1629
Cuts at: 5753 8
Size: 43
Fragments arranged by size:
1629 1425 889 287 275 267 252 185
115 103 99 90 54 50 43 18
7
EagI C'GGCC_G
Cuts at: 1537 1812 2097 2311 2696 2983 3235 1537
Size: 275 285 214 385 287 252 4090
Fragments arranged by size:
4090 385 287 285 275 252 214
EarI CTCTTCn'nnn_
Cuts at: 1353 2666 3718 1353
Size: 1313 1052 3423
Fragments arranged by size:
3423 1313 1052
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4375 4749 4895 387
Size: 1064 138 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 2096 2105 2767 3007 3851 4448
Size: 393 412 52 9 662 240 844 597
Cuts at: 4448 5235 5503 1239
Size: 787 268 1524
Fragments arranged by size:
1524 844 787 662 597 412 393 268
240 52 9
EcoO109I rG'GnC_Cy
Cuts at: 2104 2209 2605 2866 2967 2104
Size: 105 396 261 101 4925
Fragments arranged by size:
4925 396 261 105 101
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FseI GG_CCGG'CC
Cuts at: 2517 2517
Size: 5788
FspI TGC'GCA
Cuts at: 2456 2735 4294 2456
Size: 279 1559 3950
Fragments arranged by size:
3950 1559 279
HaeII r_GCGC'y
Cuts at: 1678 2048 2306 2507 2790 2828 2858 2960 4935
Size: 370 258 201 283 38 30 102 1975
Cuts at: 4935 1678
Size: 2531
Fragments arranged by size:
2531 1975 370 283 258 201 102 38
30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4429 4461 4503 4535 1373
Size: 32 135 32 2857 32 42 32 2626
Fragments arranged by size:
2857 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2899 3132 3378 669
Size: 988 281 961 233 246 3079
Fragments arranged by size:
3079 988 961 281 246 233
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KpnI G_GTAC'C
Cuts at: 3113 3113
Size: 5788
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2046 4902 5086 1305
Size: 741 2856 184 2007
Fragments arranged by size:
2856 2007 741 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3966
Fragments arranged by size:
3966 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2405 2762 3746 4105 4264 518
Size: 648 1239 357 984 359 159 2042
Fragments arranged by size:
2042 1239 984 648 359 357 159
MspA1I CmG'CkG
Cuts at: 1606 2215 2255 2284 2294 2316 2833 2904 3204
Size: 609 40 29 10 22 517 71 300
Cuts at: 3204 3881 5029 5274 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 609 517 300 245 71
40 29 22 10
NaeI GCC'GGC
Cuts at: 2515 2515
Size: 5788
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4297
Fragments arranged by size:
4297 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NgoMIV G'CCGG_C
Cuts at: 2513 2513
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 2311 3235 2311
Size: 924 4864
NspI r_CATG'y
Cuts at: 1132 1229 3146 4691 1132
Size: 97 1917 1545 2229
Fragments arranged by size:
2229 1917 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2049 1348
Size: 701 5087
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PmlI CAC'GTG
Cuts at: 2707 2707
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3857 3889 5370 5402 3059
Size: 32 766 32 1481 32 3445
Fragments arranged by size:
3445 1481 766 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 2104
Size: 5788
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PspOMI G'GGCC_C
Cuts at: 2209 2715 3054 2209
Size: 506 339 4943
Fragments arranged by size:
4943 506 339
PspXI vC'TCGA_Gb
Cuts at: 2438 2438
Size: 5788
PstI C_TGCA'G
Cuts at: 830 3140 830
Size: 2310 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
PvuII CAG'CTG
Cuts at: 2255 2904 2255
Size: 649 5139
SacII CC_GC'GG
Cuts at: 2216 2295 2216
Size: 79 5709
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 3136 4271 4952 5143 826
Size: 219 2091 1135 681 191 1471
Fragments arranged by size:
2091 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 2037 2954 3115 1872
Size: 165 917 161 4545
Fragments arranged by size:
4545 917 165 161
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2438 3892 4793 5055 820
Size: 197 926 495 1454 901 262 1553
Fragments arranged by size:
1553 1454 926 901 495 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SrfI GCCC'GGGC
Cuts at: 2954 2954
Size: 5788
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5788
StyI C'CwwG_G
Cuts at: 513 1065 2004 3227 513
Size: 552 939 1223 3074
Fragments arranged by size:
3074 1223 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3815 3832 3985 4170 4615 325
Size: 1255 2235 17 153 185 445 1498
Fragments arranged by size:
2235 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4034 5746 96
Size: 274 80 33 51 1443 2057 1712 138
Fragments arranged by size:
2057 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2558 2590 5748 5780 2558
Size: 32 3158 32 2566
Fragments arranged by size:
3158 2566 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 2438 1943
Size: 495 5293
XmaI C'CCGG_G
Cuts at: 1870 2035 2952 3113 1870
Size: 165 917 161 4545
Fragments arranged by size:
4545 917 165 161
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI
KasI NarI NruI NsiI PacI PfoI PsrI RsrII
SacI SanDI SapI SexAI SfiI SfoI SwaI XcmI