Restriction map for
pFN21AE9407
(Circular) (Six-base) MAPSORT of: pFN21AE9407.seq Check: 3276 from: 1 to: 5704
pFN21AE9407 5704 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3514 278
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3047 1937
Size: 1110 4594
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2315 3767 5151 5419 1632
Size: 683 1452 1384 268 1917
Fragments arranged by size:
1917 1452 1384 683 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 2019 2021 2307 4603 5580 1203
Size: 816 2 286 2296 977 1327
Fragments arranged by size:
2296 1327 977 816 286 2
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2360 1184
Size: 1176 4528
AlwNI CAG_nnn'CTG
Cuts at: 3447 5019 3447
Size: 1572 4132
ApaLI G'TGCA_C
Cuts at: 3761 4917 3761
Size: 1156 4548
ApoI r'AATT_y
Cuts at: 1493 2854 3013 3239 3391 5500 1493
Size: 1361 159 226 152 2109 1697
Fragments arranged by size:
2109 1697 1361 226 159 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 3029 1870
Size: 73 1086 4545
Fragments arranged by size:
4545 1086 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2008 2041 2008
Size: 33 5671
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3025 4480 618
Size: 325 217 627 1238 1455 1842
Fragments arranged by size:
1842 1455 1238 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3023 1391
Size: 1632 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2630 2911 928
Size: 971 731 281 3721
Fragments arranged by size:
3721 971 731 281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3895 3929 1903
Size: 34 50 34 1874 34 3678
Fragments arranged by size:
3678 1874 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 2433 1548
Size: 885 4819
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2263 4315 136
Size: 107 122 71 631 605 591 2052 1525
Fragments arranged by size:
2052 1525 631 605 591 122 107 71
BglII A'GATC_T
Cuts at: 2665 5699 2665
Size: 3034 2670
BlpI GC'TnA_GC
Cuts at: 3121 3121
Size: 5704
Bme1580I G_kGCm'C
Cuts at: 1790 3765 4921 1790
Size: 1975 1156 2573
Fragments arranged by size:
2573 1975 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2539 2864 3435
Size: 416 53 83 399 24 1122 325 571
Cuts at: 3435 4393 442
Size: 958 1753
Fragments arranged by size:
1753 1122 958 571 416 399 325 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4364 1239
Size: 3125 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3829 4694 4992 3829
Size: 865 298 4541
Fragments arranged by size:
4541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2108 2276 4367 882
Size: 617 609 168 2091 2219
Fragments arranged by size:
2219 2091 617 609 168
BsaAI yAC'GTr
Cuts at: 493 1204 2022 3495 493
Size: 711 818 1473 2702
Fragments arranged by size:
2702 1473 818 711
BsaBI GATnn'nnATC
Cuts at: 2649 5698 2649
Size: 3049 2655
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3511 3893 4528 275
Size: 53 83 186 936 1978 382 635 1451
Fragments arranged by size:
1978 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4137 4535 4809 4956 5367 4137
Size: 398 274 147 411 4474
Fragments arranged by size:
4474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5479
Fragments arranged by size:
5479 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 1186
Size: 1270 4434
BseYI C'CCAG_C
Cuts at: 4907 5403 4907
Size: 496 5208
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2730 1163
Size: 573 994 4137
Fragments arranged by size:
4137 994 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3154 3915 4943 1540
Size: 275 186 1153 761 1028 2301
Fragments arranged by size:
2301 1153 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3765 3850 4921 3765
Size: 85 1071 4548
Fragments arranged by size:
4548 1071 85
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3023 3765 3850 4921 1391
Size: 399 1233 742 85 1071 2174
Fragments arranged by size:
2174 1233 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 2248 3593 5323 2248
Size: 1345 1730 2629
Fragments arranged by size:
2629 1730 1345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3059 844
Size: 2215 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3591 1394
Size: 429 1768 3507
Fragments arranged by size:
3507 1768 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2190 2418 4199 4373 65
Size: 1183 942 228 1781 174 1396
Fragments arranged by size:
1781 1396 1183 942 228 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4348 4535 1700
Size: 110 2538 187 2869
Fragments arranged by size:
2869 2538 187 110
BsrGI T'GTAC_A
Cuts at: 96 2409 96
Size: 2313 3391
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 3764 4776 3764
Size: 1012 4692
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5704
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstXI CCAn_nnnn'nTGG
Cuts at: 2669 2669
Size: 5704
BstYI r'GATC_y
Cuts at: 1972 2229 2515 2665 3034 3401 3786 3803 5255
Size: 257 286 150 369 367 385 17 1452
Cuts at: 5255 5341 5353 5699 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 369 367 346 286 257
150 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2655 513
Size: 552 1590 3562
Fragments arranged by size:
3562 1590 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 2079 2636 3220 4014 4034 2079
Size: 557 584 794 20 3749
Fragments arranged by size:
3749 794 584 557 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4711 809
Size: 507 3395 1802
Fragments arranged by size:
3395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2255 3151 4040
Size: 54 1425 50 275 18 425 896 889
Cuts at: 4040 5669 8
Size: 1629 43
Fragments arranged by size:
1629 1425 896 889 425 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3151 1537
Size: 275 1339 4090
Fragments arranged by size:
4090 1339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3634 1353
Size: 2281 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4291 4665 4811 387
Size: 1064 138 2702 374 146 1280
Fragments arranged by size:
2702 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 3767 4364 5151 5419 1239
Size: 393 683 1452 597 787 268 1524
Fragments arranged by size:
1524 1452 787 683 597 393 268
EcoRI G'AATT_C
Cuts at: 2854 3013 3391 2854
Size: 159 378 5167
Fragments arranged by size:
5167 378 159
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 4851 1678
Size: 3173 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 4345 4377 4419
Size: 32 135 32 899 32 1842 32 42
Cuts at: 4419 4451 1373
Size: 32 2626
Fragments arranged by size:
2626 1842 899 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2305 2536 3048 3294 669
Size: 988 281 367 231 512 246 3079
Fragments arranged by size:
3079 988 512 367 281 246 231
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2965 4818 5002 1305
Size: 1660 1853 184 2007
Fragments arranged by size:
2007 1853 1660 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2257 10
Size: 54 1425 343 425 3457
Fragments arranged by size:
3457 1425 425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2093 2342 3662 4021 4180 518
Size: 648 927 249 1320 359 159 2042
Fragments arranged by size:
2042 1320 927 648 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 3120 3797 4945 5190 1606
Size: 1514 677 1148 245 2120
Fragments arranged by size:
2120 1514 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2655 513
Size: 552 1590 3562
Fragments arranged by size:
3562 1590 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 2281 2311 3062 4607 1132
Size: 97 1052 30 751 1545 2229
Fragments arranged by size:
2229 1545 1052 751 97 30
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 2307 4603 2307
Size: 2296 3408
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5704
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PmlI CAC'GTG
Cuts at: 2022 2022
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PstI C_TGCA'G
Cuts at: 830 3056 830
Size: 2226 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 3952 5664 3952
Size: 1712 3992
SfcI C'TryA_G
Cuts at: 826 1045 3052 4187 4868 5059 826
Size: 219 2007 1135 681 191 1471
Fragments arranged by size:
2007 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2263 2263
Size: 5704
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3808 4709 4971 820
Size: 197 926 1865 901 262 1553
Fragments arranged by size:
1865 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 2820 3628 5
Size: 47 2768 808 2081
Fragments arranged by size:
2768 2081 808 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5704
StyI C'CwwG_G
Cuts at: 513 1065 2655 3143 513
Size: 552 1590 488 3074
Fragments arranged by size:
3074 1590 552 488
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3731 3748 3901 4086 4531 325
Size: 1255 2151 17 153 185 445 1498
Fragments arranged by size:
2151 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2409 2452 3950
Size: 274 80 33 51 1443 432 43 1498
Cuts at: 3950 5662 96
Size: 1712 138
Fragments arranged by size:
1712 1498 1443 432 274 138 80 51
43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5664 5696 5664
Size: 32 5672
Tth111I GACn'n_nGTC
Cuts at: 1316 2870 1316
Size: 1554 4150
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2509 2509
Size: 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 3833 3833
Size: 5704
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3512 276
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfoI SrfI SwaI