Restriction map for
pFN21AE9405
(Circular) (Six-base) MAPSORT of: pFN21AE9405.seq Check: 1779 from: 1 to: 5785
pFN21AE9405 5785 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2007 2732 2828 3848 5232 5500 1632
Size: 375 725 96 1020 1384 268 1917
Fragments arranged by size:
1917 1384 1020 725 375 268 96
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 4684 5661 1203
Size: 3481 977 1327
Fragments arranged by size:
3481 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 2129 2361 1166
Size: 963 232 4590
Fragments arranged by size:
4590 963 232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2475 3528 5100 2475
Size: 1053 1572 3160
Fragments arranged by size:
3160 1572 1053
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 2432 3094 3320 3472 5581 1493
Size: 939 662 226 152 2109 1697
Fragments arranged by size:
2109 1697 939 662 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 3110 1870
Size: 73 1167 4545
Fragments arranged by size:
4545 1167 73
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3106 4561 618
Size: 325 217 627 1319 1455 1842
Fragments arranged by size:
1842 1455 1319 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2057 2312 2333 2526 3104 1391
Size: 666 255 21 193 578 4072
Fragments arranged by size:
4072 666 578 255 193 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 2206 928
Size: 971 199 108 4507
Fragments arranged by size:
4507 971 199 108
BbvCI CC'TCA_GC
Cuts at: 2833 2833
Size: 5785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3976 4010 1903
Size: 34 2039 34 3678
Fragments arranged by size:
3678 2039 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2434 3677 4887 2434
Size: 1243 1210 3332
Fragments arranged by size:
3332 1243 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4396 136
Size: 107 122 71 631 605 2724 1525
Fragments arranged by size:
2724 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5780 5780
Size: 5785
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 2677 2960 3846 5002 1790
Size: 887 283 886 1156 2573
Fragments arranged by size:
2573 1156 887 886 283
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3516 4474 442
Size: 416 53 83 399 24 2099 958 1753
Fragments arranged by size:
2099 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2163 4445 1239
Size: 924 2282 2579
Fragments arranged by size:
2579 2282 924
Bpu10I CC'TnA_GC
Cuts at: 2833 2833
Size: 5785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3910 4775 5073 3910
Size: 865 298 4622
Fragments arranged by size:
4622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2069 4448 882
Size: 617 570 2379 2219
Fragments arranged by size:
2379 2219 617 570
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 2210 5779 2210
Size: 3569 2216
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3592 3974 4609 275
Size: 53 83 186 936 2059 382 635 1451
Fragments arranged by size:
2059 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2327 2357 1178
Size: 30 165 30 924 30 4606
Fragments arranged by size:
4606 924 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3076 1186
Size: 1890 3895
BseYI C'CCAG_C
Cuts at: 2149 2494 4988 5484 2149
Size: 345 2494 496 2450
Fragments arranged by size:
2494 2450 496 345
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2574 1163
Size: 573 838 4374
Fragments arranged by size:
4374 838 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2333 2526 3846 3931 5002 2333
Size: 193 1320 85 1071 3116
Fragments arranged by size:
3116 1320 1071 193 85
BsmI GAATG_Cn'
Cuts at: 3296 3389 3296
Size: 93 5692
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2312 2333 2526 2677 2960 3104
Size: 399 267 255 21 193 151 283 144
Cuts at: 3104 3846 3931 5002 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 267 255 193
151 144 85 21
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 3674 5404 3674
Size: 1730 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2651 3140 844
Size: 1807 489 3489
Fragments arranged by size:
3489 1807 489
BsrBI CCG'CTC
Cuts at: 1394 1823 3672 1394
Size: 429 1849 3507
Fragments arranged by size:
3507 1849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2258 2655 4280 4454 65
Size: 1183 1010 397 1625 174 1396
Fragments arranged by size:
1625 1396 1183 1010 397 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5785
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstYI r'GATC_y
Cuts at: 1972 3115 3482 3867 3884 5336 5422 5434 5780
Size: 1143 367 385 17 1452 86 12 346
Cuts at: 5780 1972
Size: 1977
Fragments arranged by size:
1977 1452 1143 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4294
Fragments arranged by size:
4294 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5046
Fragments arranged by size:
5046 683 56
BtsI GCAGTG_nn'
Cuts at: 2356 2368 2947 3301 4095 4115 2356
Size: 12 579 354 794 20 4026
Fragments arranged by size:
4026 794 579 354 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2403 2414 2793
Size: 54 1425 50 275 18 573 11 379
Cuts at: 2793 3232 4121 5750 8
Size: 439 889 1629 43
Fragments arranged by size:
1629 1425 889 573 439 379 275 54
50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2514 3062 3715 1353
Size: 1161 548 653 3423
Fragments arranged by size:
3423 1161 653 548
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2921 4372 4746 4892 387
Size: 1064 138 1332 1451 374 146 1280
Fragments arranged by size:
1451 1332 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2331 2524 2331
Size: 193 5592
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2163 2732 2828 3848 4445 5232
Size: 393 375 156 569 96 1020 597 787
Cuts at: 5232 5500 1239
Size: 268 1524
Fragments arranged by size:
1524 1020 787 597 569 393 375 268
156 96
EcoNI CCTnn'n_nnAGG
Cuts at: 3017 3017
Size: 5785
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 5785
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 4932 1678
Size: 3254 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4426 4458 4500 4532 1373
Size: 32 135 32 2854 32 42 32 2626
Fragments arranged by size:
2854 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2392 2896 3129 3375 669
Size: 988 281 454 504 233 246 3079
Fragments arranged by size:
3079 988 504 454 281 246 233
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4899 5083 1305
Size: 3594 184 2007
Fragments arranged by size:
3594 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2405 2416 2795 10
Size: 54 1425 343 573 11 379 3000
Fragments arranged by size:
3000 1425 573 379 343 54 11
MslI CAynn'nnrTG
Cuts at: 518 1166 2085 2129 2361 2381 3743 4102 4261
Size: 648 919 44 232 20 1362 359 159
Cuts at: 4261 518
Size: 2042
Fragments arranged by size:
2042 1362 919 648 359 232 159 44
20
MspA1I CmG'CkG
Cuts at: 1606 2129 2889 3201 3878 5026 5271 1606
Size: 523 760 312 677 1148 245 2120
Fragments arranged by size:
2120 1148 760 677 523 312 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4294
Fragments arranged by size:
4294 939 552
NdeI CA'TA_TG
Cuts at: 387 2455 2585 387
Size: 2068 130 3587
Fragments arranged by size:
3587 2068 130
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 3143 4688 1132
Size: 97 1914 1545 2229
Fragments arranged by size:
2229 1914 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2412 2475 1108
Size: 1304 63 4418
Fragments arranged by size:
4418 1304 63
PfoI T'CCnGG_A
Cuts at: 2426 2426
Size: 5785
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2811 2843 2811
Size: 32 5753
PstI C_TGCA'G
Cuts at: 830 2363 3137 830
Size: 1533 774 3478
Fragments arranged by size:
3478 1533 774
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
PvuII CAG'CTG
Cuts at: 2129 2889 2129
Size: 760 5025
SacI G_AGCT'C
Cuts at: 2333 2526 2333
Size: 193 5592
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SfcI C'TryA_G
Cuts at: 826 1045 2359 3133 4268 4949 5140 826
Size: 219 1314 774 1135 681 191 1471
Fragments arranged by size:
1471 1314 1135 774 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3889 4790 5052 820
Size: 197 926 1946 901 262 1553
Fragments arranged by size:
1946 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5785
StyI C'CwwG_G
Cuts at: 513 1065 2004 2871 3224 513
Size: 552 939 867 353 3074
Fragments arranged by size:
3074 939 867 552 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2260 3812 3829 3982 4167 4612 325
Size: 1255 680 1552 17 153 185 445 1498
Fragments arranged by size:
1552 1498 1255 680 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4031 5743 96
Size: 274 80 33 51 1443 2054 1712 138
Fragments arranged by size:
2054 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2434 2466 2656 2688 5745 5777 2434
Size: 32 190 32 3057 32 2442
Fragments arranged by size:
3057 2442 190 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI