Restriction map for
pFN21AE9399
(Circular) (Six-base) MAPSORT of: pFN21AE9399.seq Check: 4400 from: 1 to: 12850
pFN21AE9399 12850 bp
With 182 enzymes: SgfI *
August 18, 2017 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6179 6179
Size: 12850
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5952 9722 10660 278
Size: 53 83 186 936 4416 3770 938 2468
Fragments arranged by size:
4416 3770 2468 938 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5007 9741 10193 1937
Size: 3070 4734 452 4594
Fragments arranged by size:
4734 4594 3070 452
Acc65I G'GTAC_C
Cuts at: 10171 10171
Size: 12850
AclI AA'CG_TT
Cuts at: 1563 10149 10977 11350 1563
Size: 8586 828 373 3063
Fragments arranged by size:
8586 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3587 4001 4047 4557 4686 6518 6959 7935
Size: 1955 414 46 510 129 1832 441 976
Cuts at: 7935 10913 12297 12565 1632
Size: 2978 1384 268 1917
Fragments arranged by size:
2978 1955 1917 1832 1384 976 510 441
414 268 129 46
AfeI AGC'GCT
Cuts at: 5813 6368 8155 10130 5813
Size: 555 1787 1975 8533
Fragments arranged by size:
8533 1975 1787 555
AflII C'TTAA_G
Cuts at: 820 1017 9710 820
Size: 197 8693 3960
Fragments arranged by size:
8693 3960 197
AflIII A'CryG_T
Cuts at: 1203 4612 4924 5553 6826 9570 11749 12726 1203
Size: 3409 312 629 1273 2744 2179 977 1327
Fragments arranged by size:
3409 2744 2179 1327 1273 977 629 312
AgeI A'CCGG_T
Cuts at: 11681 11681
Size: 12850
AhdI GACnn_n'nnGTC
Cuts at: 4939 11579 4939
Size: 6640 6210
AleI CACnn'nnGTG
Cuts at: 1166 5588 5766 9966 1166
Size: 4422 178 4200 4050
Fragments arranged by size:
4422 4200 4050 178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2710 4901 6083 6446 9957 10121 1184
Size: 1526 2191 1182 363 3511 164 3913
Fragments arranged by size:
3913 3511 2191 1526 1182 363 164
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4364 4396 6860 6892 4364
Size: 32 2464 32 10322
Fragments arranged by size:
10322 2464 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2581 2831 3085 4216 4225 4249 4799 4826 5357
Size: 250 254 1131 9 24 550 27 531
Cuts at: 5357 5976 6262 7017 7525 10593 12165 2581
Size: 619 286 755 508 3068 1572 3266
Fragments arranged by size:
3266 3068 1572 1131 755 619 550 531
508 286 254 250 27 24 9
ApaI G_GGCC'C
Cuts at: 2312 2605 2820 3952 4982 5166 5196 5303 5603
Size: 293 215 1132 1030 184 30 107 300
Cuts at: 5603 5850 6229 6287 7928 8063 8314 8512 9057
Size: 247 379 58 1641 135 251 198 545
Cuts at: 9057 2312
Size: 6105
Fragments arranged by size:
6105 1641 1132 1030 545 379 300 293
251 247 215 198 184 135 107 58
30
ApaLI G'TGCA_C
Cuts at: 5507 5986 6395 6747 7418 9559 10907 12063 5507
Size: 479 409 352 671 2141 1348 1156 6294
Fragments arranged by size:
6294 2141 1348 1156 671 479 409 352
ApoI r'AATT_y
Cuts at: 1493 5753 7341 10159 10385 10537 12646 1493
Size: 4260 1588 2818 226 152 2109 1697
Fragments arranged by size:
4260 2818 2109 1697 1588 226 152
AseI AT'TA_AT
Cuts at: 160 1032 11404 160
Size: 872 10372 1606
Fragments arranged by size:
10372 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12850
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2065 2186 2311 2451 2661 3459
Size: 73 93 29 121 125 140 210 798
Cuts at: 3459 3849 4083 4580 4650 5166 5195 5294 5328
Size: 390 234 497 70 516 29 99 34
Cuts at: 5328 5862 6906 7005 8062 8238 9339 9534 9987
Size: 534 1044 99 1057 176 1101 195 453
Cuts at: 9987 10175 1870
Size: 188 4545
Fragments arranged by size:
4545 1101 1057 1044 798 534 516 497
453 390 234 210 195 188 176 140
125 121 99 99 93 73 70 34
29 29
AvrII C'CTAG_G
Cuts at: 8365 8365
Size: 12850
BamHI G'GATC_C
Cuts at: 5786 6679 6706 10180 10547 5786
Size: 893 27 3474 367 8089
Fragments arranged by size:
8089 3474 893 367 27
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2608 3476 4660 5421
Size: 325 217 627 294 527 868 1184 761
Cuts at: 5421 5880 6947 7335 7825 8419 9150 10121 10171
Size: 459 1067 388 490 594 731 971 50
Cuts at: 10171 11626 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1184 1067 971 868 761 731
627 594 527 490 459 388 325 294
217 50
BanII G_rGCy'C
Cuts at: 1391 2187 2223 2312 2605 2643 2760 2820 2907
Size: 796 36 89 293 38 117 60 87
Cuts at: 2907 3059 3952 4024 4177 4469 4818 4982 5166
Size: 152 893 72 153 292 349 164 184
Cuts at: 5166 5196 5295 5303 5603 5850 6117 6229 6272
Size: 30 99 8 300 247 267 112 43
Cuts at: 6272 6287 6492 6933 7446 7498 7594 7928 8010
Size: 15 205 441 513 52 96 334 82
Cuts at: 8010 8063 8170 8314 8512 8553 9033 9057 9179
Size: 53 107 144 198 41 480 24 122
Cuts at: 9179 9451 9975 10062 10169 1391
Size: 272 524 87 107 4072
Fragments arranged by size:
4072 893 796 524 513 480 441 349
334 300 293 292 272 267 247 205
198 184 164 153 152 144 122 117
112 107 107 99 96 89 87 87
82 72 60 53 52 43 41 38
36 30 24 15 8
BbeI G_GCGC'C
Cuts at: 2085 5425 5884 6951 8423 10125 2085
Size: 3340 459 1067 1472 1702 4810
Fragments arranged by size:
4810 3340 1702 1472 1067 459
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3534 3657 5084 6048 6132 6850 7036
Size: 971 1635 123 1427 964 84 718 186
Cuts at: 7036 7923 9722 928
Size: 887 1799 4056
Fragments arranged by size:
4056 1799 1635 1427 971 964 887 718
186 123 84
BbvCI CC'TCA_GC
Cuts at: 3165 4109 6685 6980 7815 8220 3165
Size: 944 2576 295 835 405 7795
Fragments arranged by size:
7795 2576 944 835 405 295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8219 8253 11041 11075 1903
Size: 34 6282 34 2788 34 3678
Fragments arranged by size:
6282 3678 2788 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4720 8923 10742 11952 4720
Size: 4203 1819 1210 5618
Fragments arranged by size:
5618 4203 1819 1210
BclI T'GATC_A
Cuts at: 1548 2916 1548
Size: 1368 11482
BfrBI ATG'CAT
Cuts at: 5825 9934 5825
Size: 4109 8741
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2868 2877
Size: 107 122 71 631 605 337 859 9
Cuts at: 2877 6205 7363 8036 8058 11461 136
Size: 3328 1158 673 22 3403 1525
Fragments arranged by size:
3403 3328 1525 1158 859 673 631 605
337 122 107 71 22 9
BglII A'GATC_T
Cuts at: 3984 4509 12845 3984
Size: 525 8336 3989
Fragments arranged by size:
8336 3989 525
BlpI GC'TnA_GC
Cuts at: 4071 4209 4463 6266 8795 10267 4071
Size: 138 254 1803 2529 1472 6654
Fragments arranged by size:
6654 2529 1803 1472 254 138
Bme1580I G_kGCm'C
Cuts at: 1790 2312 2605 2820 3479 3607 3763 3952 4690
Size: 522 293 215 659 128 156 189 738
Cuts at: 4690 4982 5166 5196 5303 5511 5603 5850 5990
Size: 292 184 30 107 208 92 247 140
Cuts at: 5990 6229 6287 6399 6751 7006 7422 7608 7830
Size: 239 58 112 352 255 416 186 222
Cuts at: 7830 7928 8063 8252 8314 8512 9057 9563 10911
Size: 98 135 189 62 198 545 506 1348
Cuts at: 10911 12067 1790
Size: 1156 2573
Fragments arranged by size:
2573 1348 1156 738 659 545 522 506
416 352 293 292 255 247 239 222
215 208 198 189 189 186 184 156
140 135 128 112 107 98 92 62
58 30
BmgBI CAC'GTC
Cuts at: 1318 5764 6829 9665 1318
Size: 4446 1065 2836 4503
Fragments arranged by size:
4503 4446 2836 1065
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2400 3302 3776
Size: 416 53 83 399 24 983 902 474
Cuts at: 3776 5953 6100 6298 8639 9139 10581 11539 442
Size: 2177 147 198 2341 500 1442 958 1753
Fragments arranged by size:
2341 2177 1753 1442 983 958 902 500
474 416 399 198 147 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12850
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3496 4588 5224 6133 7525 3496
Size: 1092 636 909 1392 8821
Fragments arranged by size:
8821 1392 1092 909 636
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3213 3385 4050 4161 4491 4848 5115 5714
Size: 1974 172 665 111 330 357 267 599
Cuts at: 5714 5822 5916 5982 6215 6239 6492 6657 6749
Size: 108 94 66 233 24 253 165 92
Cuts at: 6749 7318 7367 7409 7512 7551 8184 9044 9698
Size: 569 49 42 103 39 633 860 654
Cuts at: 9698 11510 1239
Size: 1812 2579
Fragments arranged by size:
2579 1974 1812 860 665 654 633 599
569 357 330 267 253 233 172 165
111 108 103 94 92 66 49 42
39 24
Bpu10I CC'TnA_GC
Cuts at: 2754 3165 4035 4077 4109 6218 6685 6980 7500
Size: 411 870 42 32 2109 467 295 520
Cuts at: 7500 7815 7820 8220 8547 9650 2754
Size: 315 5 400 327 1103 5954
Fragments arranged by size:
5954 2109 1103 870 520 467 411 400
327 315 295 42 32 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4376 5393 6866 9158 10975 11840 12138 4376
Size: 1017 1473 2292 1817 865 298 5088
Fragments arranged by size:
5088 2292 1817 1473 1017 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2664 3685 6446 6644 7704 11513 882
Size: 617 1165 1021 2761 198 1060 3809 2219
Fragments arranged by size:
3809 2761 2219 1165 1060 1021 617 198
BsaAI yAC'GTr
Cuts at: 493 1204 3396 3758 4925 10000 10641 493
Size: 711 2192 362 1167 5075 641 2702
Fragments arranged by size:
5075 2702 2192 1167 711 641 362
BsaBI GATnn'nnATC
Cuts at: 9806 12844 9806
Size: 3038 9812
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 4739 5317 5422
Size: 53 83 186 936 549 2657 578 105
Cuts at: 5422 5881 5949 6531 6948 8420 9719 9924 10122
Size: 459 68 582 417 1472 1299 205 198
Cuts at: 10122 10657 11039 11674 275
Size: 535 382 635 1451
Fragments arranged by size:
2657 1472 1451 1299 936 635 582 578
549 535 459 417 382 205 198 186
105 83 68 53
BsaWI w'CCGG_w
Cuts at: 3523 4530 5010 9088 9552 11283 11681 11955 12102
Size: 1007 480 4078 464 1731 398 274 147
Cuts at: 12102 12513 3523
Size: 411 3860
Fragments arranged by size:
4078 3860 1731 1007 480 464 411 398
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3259 3289 3469 3499 3994
Size: 30 165 30 1856 30 180 30 495
Cuts at: 3994 4024 4364 4394 6860 6890 7268 7298 7937
Size: 30 340 30 2466 30 378 30 639
Cuts at: 7937 7967 9290 9320 1178
Size: 30 1323 30 4708
Fragments arranged by size:
4708 2466 1856 1323 639 495 378 340
180 165 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2351 2378 2381 2481 2484 3182 3216
Size: 1162 3 27 3 100 3 698 34
Cuts at: 3216 3234 3264 3366 3371 3474 3601 3759 3855
Size: 18 30 102 5 103 127 158 96
Cuts at: 3855 3913 3996 3999 4002 4038 4379 4385 4499
Size: 58 83 3 3 36 341 6 114
Cuts at: 4499 5186 5213 5301 5375 5711 6458 6878 7415
Size: 687 27 88 74 336 747 420 537
Cuts at: 7415 7554 7788 8093 8096 8417 8711 9518 10070
Size: 139 234 305 3 321 294 807 552
Cuts at: 10070 1186
Size: 3966
Fragments arranged by size:
3966 1162 807 747 698 687 552 537
420 341 336 321 305 294 234 158
139 127 114 103 102 100 96 88
83 74 58 36 34 30 27 27
18 6 5 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 2215 2304 2433 3388 3431 3500 3871 4242 5156
Size: 89 129 955 43 69 371 371 914
Cuts at: 5156 5718 5849 5991 6185 7723 7751 7996 8033
Size: 562 131 142 194 1538 28 245 37
Cuts at: 8033 8150 8470 8593 8618 9035 9450 9591 12053
Size: 117 320 123 25 417 415 141 2462
Cuts at: 12053 12549 2215
Size: 496 2516
Fragments arranged by size:
2516 2462 1538 955 914 562 496 417
415 371 371 320 245 194 142 141
131 129 123 117 89 69 43 37
28 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2594 4427 4949 6283 6314 8249 9665
Size: 573 858 1833 522 1334 31 1935 1416
Cuts at: 9665 1163
Size: 4348
Fragments arranged by size:
4348 1935 1833 1416 1334 858 573 522
31
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2075 2893 8863 10300 11061 12089
Size: 275 186 74 818 5970 1437 761 1028
Cuts at: 12089 1540
Size: 2301
Fragments arranged by size:
5970 2301 1437 1028 818 761 275 186
74
BsiHKAI G_wGCw'C
Cuts at: 3401 5511 5583 5990 6255 6272 6399 6751 6933
Size: 2110 72 407 265 17 127 352 182
Cuts at: 6933 7197 7286 7304 7422 7498 9179 9563 9606
Size: 264 89 18 118 76 1681 384 43
Cuts at: 9606 10062 10911 10996 12067 3401
Size: 456 849 85 1071 4184
Fragments arranged by size:
4184 2110 1681 1071 849 456 407 384
352 265 264 182 127 118 89 85
76 72 43 18 17
BsmI GAATG_Cn'
Cuts at: 6692 10361 10454 6692
Size: 3669 93 9088
Fragments arranged by size:
9088 3669 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3796 3796
Size: 12850
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2223 2312 2605 2643 2760 2820
Size: 399 397 36 89 293 38 117 60
Cuts at: 2820 2907 3059 3401 3479 3607 3763 3952 4024
Size: 87 152 342 78 128 156 189 72
Cuts at: 4024 4177 4469 4690 4818 4982 5166 5196 5295
Size: 153 292 221 128 164 184 30 99
Cuts at: 5295 5303 5511 5583 5603 5850 5990 6117 6229
Size: 8 208 72 20 247 140 127 112
Cuts at: 6229 6255 6272 6287 6399 6492 6751 6933 7006
Size: 26 17 15 112 93 259 182 73
Cuts at: 7006 7197 7286 7304 7422 7446 7498 7594 7608
Size: 191 89 18 118 24 52 96 14
Cuts at: 7608 7830 7928 8010 8063 8170 8252 8314 8512
Size: 222 98 82 53 107 82 62 198
Cuts at: 8512 8553 9033 9057 9179 9451 9563 9606 9975
Size: 41 480 24 122 272 112 43 369
Cuts at: 9975 10062 10169 10911 10996 12067 1391
Size: 87 107 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 480 399 397 369 342
293 292 272 259 247 222 221 208
198 191 189 184 182 164 156 153
152 140 128 128 127 122 118 117
112 112 112 107 107 99 98 96
93 89 89 87 87 85 82 82
78 73 72 72 62 60 53 52
43 41 38 36 30 26 24 24
20 18 17 15 14 8
BspEI T'CCGG_A
Cuts at: 3523 9088 9552 12513 3523
Size: 5565 464 2961 3860
Fragments arranged by size:
5565 3860 2961 464
BspHI T'CATG_A
Cuts at: 10739 12469 10739
Size: 1730 11120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6179 8337 9138 10205 844
Size: 5335 2158 801 1067 3489
Fragments arranged by size:
5335 3489 2158 1067 801
BsrBI CCG'CTC
Cuts at: 1394 1823 3448 3609 4567 5707 6222 9834 10737
Size: 429 1625 161 958 1140 515 3612 903
Cuts at: 10737 1394
Size: 3507
Fragments arranged by size:
3612 3507 1625 1140 958 903 515 429
161
BsrDI GCAATG_nn'
Cuts at: 65 1248 9946 11345 11519 65
Size: 1183 8698 1399 174 1396
Fragments arranged by size:
8698 1399 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2070 2084 2238 2258 3414 5370 8862
Size: 110 260 14 154 20 1156 1956 3492
Cuts at: 8862 11494 11681 1700
Size: 2632 187 2869
Fragments arranged by size:
3492 2869 2632 1956 1156 260 187 154
110 20 14
BsrGI T'GTAC_A
Cuts at: 96 6026 6165 6801 96
Size: 5930 139 636 6145
Fragments arranged by size:
6145 5930 636 139
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12850
BssSI C'ACGA_G
Cuts at: 5642 10910 11922 5642
Size: 5268 1012 6570
Fragments arranged by size:
6570 5268 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4225 4234 5757 9601 9885 794
Size: 3431 9 1523 3844 284 3759
Fragments arranged by size:
3844 3759 3431 1523 284 9
BstBI TT'CG_AA
Cuts at: 11654 11654
Size: 12850
BstEII G'GTnAC_C
Cuts at: 1184 4135 1184
Size: 2951 9899
BstXI CCAn_nnnn'nTGG
Cuts at: 5768 6280 7759 7785 7996 9676 5768
Size: 512 1479 26 211 1680 8942
Fragments arranged by size:
8942 1680 1479 512 211 26
BstYI r'GATC_y
Cuts at: 1972 3038 3984 4509 5786 5889 6135 6679 6706
Size: 1066 946 525 1277 103 246 544 27
Cuts at: 6706 10180 10547 10932 10949 12401 12487 12499 12845
Size: 3474 367 385 17 1452 86 12 346
Cuts at: 12845 1972
Size: 1977
Fragments arranged by size:
3474 1977 1452 1277 1066 946 544 525
385 367 346 246 103 86 27 17
12
BstZ17I GTA'TAC
Cuts at: 5008 5008
Size: 12850
Bsu36I CC'TnA_GG
Cuts at: 3815 4573 5112 5358 7867 8322 8937 9609 3815
Size: 758 539 246 2509 455 615 672 7056
Fragments arranged by size:
7056 2509 758 672 615 539 455 246
BtgI C'CryG_G
Cuts at: 513 1065 2004 2075 2101 2264 2863 2872 2887
Size: 552 939 71 26 163 599 9 15
Cuts at: 2887 3510 3540 4656 6951 7358 8040 8053 8682
Size: 623 30 1116 2295 407 682 13 629
Cuts at: 8682 8736 9523 9687 9757 513
Size: 54 787 164 70 3606
Fragments arranged by size:
3606 2295 1116 939 787 682 629 623
599 552 407 164 163 71 70 54
30 26 15 13 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 12111
Fragments arranged by size:
12111 683 56
BtsI GCAGTG_nn'
Cuts at: 2350 4095 4859 5003 5345 6077 6287 6635 7535
Size: 1745 764 144 342 732 210 348 900
Cuts at: 7535 8806 8962 9268 10366 11160 11180 2350
Size: 1271 156 306 1098 794 20 4020
Fragments arranged by size:
4020 1745 1271 1098 900 794 764 732
348 342 306 210 156 144 20
ClaI AT'CG_AT
Cuts at: 1554 4422 1554
Size: 2868 9982
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5764 5799 529
Size: 35 5200 35 7580
Fragments arranged by size:
7580 5200 35 35
DraI TTT'AAA
Cuts at: 10154 10154
Size: 12850
DraIII CAC_nnn'GTG
Cuts at: 3758 5736 6094 6306 9601 3758
Size: 1978 358 212 3295 7007
Fragments arranged by size:
7007 3295 1978 358 212
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5778 7040 7743 8128 11857 809
Size: 507 4462 1262 703 385 3729 1802
Fragments arranged by size:
4462 3729 1802 1262 703 507 385
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2072 2681 2890
Size: 54 1425 50 275 18 242 609 209
Cuts at: 2890 4491 6379 7355 7541 7986 8860 9429 9690
Size: 1601 1888 976 186 445 874 569 261
Cuts at: 9690 10297 11186 12815 8
Size: 607 889 1629 43
Fragments arranged by size:
1888 1629 1601 1425 976 889 874 609
607 569 445 275 261 242 209 186
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2072 2890 8860 10297 1537
Size: 275 260 818 5970 1437 4090
Fragments arranged by size:
5970 4090 1437 818 275 260
EarI CTCTTCn'nnn_
Cuts at: 1353 3025 3096 3135 3189 3401 3794 7268 7368
Size: 1672 71 39 54 212 393 3474 100
Cuts at: 7368 8013 9180 9627 10780 1353
Size: 645 1167 447 1153 3423
Fragments arranged by size:
3474 3423 1672 1167 1153 645 447 393
212 100 71 54 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2121 2190 2213 2234 3458 6866
Size: 1064 138 532 69 23 21 1224 3408
Cuts at: 6866 7103 8122 8741 11437 11811 11957 387
Size: 237 1019 619 2696 374 146 1280
Fragments arranged by size:
3408 2696 1280 1224 1064 1019 619 532
374 237 146 138 69 23 21
EcoICRI GAG'CTC
Cuts at: 6270 6931 7496 9177 10060 6270
Size: 661 565 1681 883 9060
Fragments arranged by size:
9060 1681 883 661 565
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3213 3385 3587 4001 4047 4050 4161
Size: 393 1581 172 202 414 46 3 111
Cuts at: 4161 4491 4557 4686 4848 5115 5714 5822 5916
Size: 330 66 129 162 267 599 108 94
Cuts at: 5916 5982 6215 6239 6492 6518 6657 6749 6959
Size: 66 233 24 253 26 139 92 210
Cuts at: 6959 7318 7367 7409 7512 7551 7935 8184 9044
Size: 359 49 42 103 39 384 249 860
Cuts at: 9044 9698 10913 11510 12297 12565 1239
Size: 654 1215 597 787 268 1524
Fragments arranged by size:
1581 1524 1215 860 787 654 599 597
414 393 384 359 330 268 267 253
249 233 210 202 172 162 139 129
111 108 103 94 92 66 66 49
46 42 39 26 24 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2698 3928 4253 4593 4643 5429 5696 6245 7866
Size: 1230 325 340 50 786 267 549 1621
Cuts at: 7866 2698
Size: 7682
Fragments arranged by size:
7682 1621 1230 786 549 340 325 267
50
EcoO109I rG'GnC_Cy
Cuts at: 2230 2308 2690 2816 2828 2835 3518 3933 3949
Size: 78 382 126 12 7 683 415 16
Cuts at: 3949 4523 4577 4598 4711 4796 4833 4941 4978
Size: 574 54 21 113 85 37 108 37
Cuts at: 4978 4979 5162 5163 5192 5299 5300 5569 5599
Size: 1 183 1 29 107 1 269 30
Cuts at: 5599 5600 5846 6214 6225 6226 6238 6259 6338
Size: 1 246 368 11 1 12 21 79
Cuts at: 6338 6733 7459 7629 8059 8287 8310 8326 8372
Size: 395 726 170 430 228 23 16 46
Cuts at: 8372 8508 8509 8714 9004 9054 9109 9220 2230
Size: 136 1 205 290 50 55 111 5860
Fragments arranged by size:
5860 726 683 574 430 415 395 382
368 290 269 246 228 205 183 170
136 126 113 111 108 107 85 79
78 55 54 50 46 37 37 30
29 23 21 21 16 16 12 12
11 7 1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 7341 10159 10537 7341
Size: 2818 378 9654
Fragments arranged by size:
9654 2818 378
EcoRV GAT'ATC
Cuts at: 5266 5266
Size: 12850
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7060 7695 9580 7060
Size: 635 1885 10330
Fragments arranged by size:
10330 1885 635
FseI GG_CCGG'CC
Cuts at: 2074 2262 3418 2074
Size: 188 1156 11506
Fragments arranged by size:
11506 1156 188
FspI TGC'GCA
Cuts at: 4889 6076 6627 11356 4889
Size: 1187 551 4729 6383
Fragments arranged by size:
6383 4729 1187 551
FspAI rTGC'GCAy
Cuts at: 4889 6076 4889
Size: 1187 11663
HaeII r_GCGC'y
Cuts at: 1678 2085 2450 4858 5239 5425 5815 5884 6370
Size: 407 365 2408 381 186 390 69 486
Cuts at: 6370 6951 7324 7507 7560 8157 8423 10125 10132
Size: 581 373 183 53 597 266 1702 7
Cuts at: 10132 11997 1678
Size: 1865 2531
Fragments arranged by size:
2531 2408 1865 1702 597 581 486 407
390 381 373 365 266 186 183 69
53 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2339 2371 3259 3291 3795
Size: 32 135 32 767 32 888 32 504
Cuts at: 3795 3827 4192 4224 4512 4544 4715 4718 4747
Size: 32 365 32 288 32 171 3 29
Cuts at: 4747 4750 4925 4957 6761 6793 7937 7969 8069
Size: 3 175 32 1804 32 1144 32 100
Cuts at: 8069 8101 8318 8350 9179 9211 9738 9770 11491
Size: 32 217 32 829 32 527 32 1721
Cuts at: 11491 11523 11565 11597 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1804 1721 1144 888 829 767 527
504 365 288 217 175 171 135 100
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2996 4951 6832 8587 9657 9843
Size: 988 281 1058 1955 1881 1755 1070 186
Cuts at: 9843 10194 10440 669
Size: 351 246 3079
Fragments arranged by size:
3079 1955 1881 1755 1070 1058 988 351
281 246 186
HindIII A'AGCT_T
Cuts at: 748 748
Size: 12850
HpaI GTT'AAC
Cuts at: 10440 10440
Size: 12850
KasI G'GCGC_C
Cuts at: 2081 5421 5880 6947 8419 10121 2081
Size: 3340 459 1067 1472 1702 4810
Fragments arranged by size:
4810 3340 1702 1472 1067 459
KpnI G_GTAC'C
Cuts at: 10175 10175
Size: 12850
MfeI C'AATT_G
Cuts at: 2957 10449 2957
Size: 7492 5358
MluI A'CGCG_T
Cuts at: 12726 12726
Size: 12850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2382 3016 3518 4539 5300 5882 8722 11964
Size: 1077 634 502 1021 761 582 2840 3242
Cuts at: 11964 12148 1305
Size: 184 2007
Fragments arranged by size:
3242 2840 2007 1077 1021 761 634 582
502 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6381 9692 10
Size: 54 1425 343 4549 3311 3168
Fragments arranged by size:
4549 3311 3168 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2388 5588 5766 6428 6621 7190 7783
Size: 648 1222 3200 178 662 193 569 593
Cuts at: 7783 7946 9411 9692 9939 9966 9998 10808 11167
Size: 163 1465 281 247 27 32 810 359
Cuts at: 11167 11326 518
Size: 159 2042
Fragments arranged by size:
3200 2042 1465 1222 810 662 648 593
569 359 281 247 193 178 163 159
32 27
MspA1I CmG'CkG
Cuts at: 1606 2077 2103 2266 2275 2735 2799 2889 3182
Size: 471 26 163 9 460 64 90 293
Cuts at: 3182 3439 4070 4282 4357 4462 6204 8033 8185
Size: 257 631 212 75 105 1742 1829 152
Cuts at: 8185 8474 9035 10266 10943 12091 12336 1606
Size: 289 561 1231 677 1148 245 2120
Fragments arranged by size:
2120 1829 1742 1231 1148 677 631 561
471 460 293 289 257 245 212 163
152 105 90 75 64 26 9
NaeI GCC'GGC
Cuts at: 2072 2086 2240 2260 3416 5372 2072
Size: 14 154 20 1156 1956 9550
Fragments arranged by size:
9550 1956 1156 154 20 14
NarI GG'CG_CC
Cuts at: 2082 5422 5881 6948 8420 10122 2082
Size: 3340 459 1067 1472 1702 4810
Fragments arranged by size:
4810 3340 1702 1472 1067 459
NcoI C'CATG_G
Cuts at: 513 1065 2004 4656 7358 8040 9523 9687 513
Size: 552 939 2652 2702 682 1483 164 3676
Fragments arranged by size:
3676 2702 2652 1483 939 682 552 164
NdeI CA'TA_TG
Cuts at: 387 387
Size: 12850
NgoMIV G'CCGG_C
Cuts at: 2070 2084 2238 2258 3414 5370 2070
Size: 14 154 20 1156 1956 9550
Fragments arranged by size:
9550 1956 1156 154 20 14
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12850
NotI GC'GGCC_GC
Cuts at: 10297 10297
Size: 12850
NsiI A_TGCA'T
Cuts at: 5827 9936 5827
Size: 4109 8741
NspI r_CATG'y
Cuts at: 1132 1229 2637 4616 6101 6896 9350 9574 10208
Size: 97 1408 1979 1485 795 2454 224 634
Cuts at: 10208 11753 1132
Size: 1545 2229
Fragments arranged by size:
2454 2229 1979 1545 1485 1408 795 634
224 97
PasI CC'CwG_GG
Cuts at: 1348 4639 4799 5091 5189 7222 8504 8513 1348
Size: 3291 160 292 98 2033 1282 9 5685
Fragments arranged by size:
5685 3291 2033 1282 292 160 98 9
PciI A'CATG_T
Cuts at: 4612 9570 11749 4612
Size: 4958 2179 5713
Fragments arranged by size:
5713 4958 2179
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2831 5475 5647 6549 8697 8951 1108
Size: 1723 2644 172 902 2148 254 5007
Fragments arranged by size:
5007 2644 2148 1723 902 254 172
PfoI T'CCnGG_A
Cuts at: 2824 3401 3926 4698 5799 7026 7068 7622 8013
Size: 577 525 772 1101 1227 42 554 391
Cuts at: 8013 8696 9060 2824
Size: 683 364 6614
Fragments arranged by size:
6614 1227 1101 772 683 577 554 525
391 364 42
PmeI GTTT'AAAC
Cuts at: 10154 10154
Size: 12850
PmlI CAC'GTG
Cuts at: 3758 10000 3758
Size: 6242 6608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 5636 5668 6860 6892 9257 9289 10919
Size: 32 1240 32 1192 32 2365 32 1630
Cuts at: 10919 10951 12432 12464 4364
Size: 32 1481 32 4750
Fragments arranged by size:
4750 2365 1630 1481 1240 1192 32 32
32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2828 2835 4577 4598 4711 4833 4941 6338 6733
Size: 7 1742 21 113 122 108 1397 395
Cuts at: 6733 7459 8287 8326 8372 9004 9109 2828
Size: 726 828 39 46 632 105 6569
Fragments arranged by size:
6569 1742 1397 828 726 632 395 122
113 108 105 46 39 21 7
PshAI GACnn'nnGTC
Cuts at: 1613 2959 6738 6827 1613
Size: 1346 3779 89 7636
Fragments arranged by size:
7636 3779 1346 89
PsiI TTA'TAA
Cuts at: 10420 10420
Size: 12850
PspOMI G'GGCC_C
Cuts at: 2308 2601 2816 3948 4978 5162 5192 5299 5599
Size: 293 215 1132 1030 184 30 107 300
Cuts at: 5599 5846 6225 6283 7924 8059 8310 8508 9053
Size: 247 379 58 1641 135 251 198 545
Cuts at: 9053 2308
Size: 6105
Fragments arranged by size:
6105 1641 1132 1030 545 379 300 293
251 247 215 198 184 135 107 58
30
PstI C_TGCA'G
Cuts at: 830 2699 4221 4230 4287 5220 5394 7399 8302
Size: 1869 1522 9 57 933 174 2005 903
Cuts at: 8302 10202 830
Size: 1900 3478
Fragments arranged by size:
3478 2005 1900 1869 1522 933 903 174
57 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12850
PvuII CAG'CTG
Cuts at: 2735 2799 3182 4070 4282 8185 8474 2735
Size: 64 383 888 212 3903 289 7111
Fragments arranged by size:
7111 3903 888 383 289 212 64
RsrII CG'GwC_CG
Cuts at: 6176 6460 6176
Size: 284 12566
SacI G_AGCT'C
Cuts at: 6272 6933 7498 9179 10062 6272
Size: 661 565 1681 883 9060
Fragments arranged by size:
9060 1681 883 661 565
SacII CC_GC'GG
Cuts at: 2078 2104 2267 2890 2078
Size: 26 163 623 12038
Fragments arranged by size:
12038 623 163 26
SalI G'TCGA_C
Cuts at: 1936 10192 1936
Size: 8256 4594
SanDI GG'GwC_CC
Cuts at: 2835 4833 2835
Size: 1998 10852
SapI GCTCTTCn'nnn_
Cuts at: 7268 7368 9180 7268
Size: 100 1812 10938
Fragments arranged by size:
10938 1812 100
SbfI CC_TGCA'GG
Cuts at: 2699 4230 10202 2699
Size: 1531 5972 5347
Fragments arranged by size:
5972 5347 1531
ScaI AGT'ACT
Cuts at: 6548 9815 11098 12810 6548
Size: 3267 1283 1712 6588
Fragments arranged by size:
6588 3267 1712 1283
SexAI A'CCwGG_T
Cuts at: 5732 5732
Size: 12850
SfcI C'TryA_G
Cuts at: 826 1045 2695 3159 4217 4226 4283 5216 5390
Size: 219 1650 464 1058 9 57 933 174
Cuts at: 5390 7395 8298 9742 10198 11333 12014 12205 826
Size: 2005 903 1444 456 1135 681 191 1471
Fragments arranged by size:
2005 1650 1471 1444 1135 1058 933 903
681 464 456 219 191 174 57 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 7363 7363
Size: 12850
SfoI GGC'GCC
Cuts at: 2083 5423 5882 6949 8421 10123 2083
Size: 3340 459 1067 1472 1702 4810
Fragments arranged by size:
4810 3340 1702 1472 1067 459
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12850
SgrAI Cr'CCGG_yG
Cuts at: 1700 2084 1700
Size: 384 12466
SmaI CCC'GGG
Cuts at: 1872 2038 2067 2188 3851 4652 5168 5296 5864
Size: 166 29 121 1663 801 516 128 568
Cuts at: 5864 7007 8064 10177 1872
Size: 1143 1057 2113 4545
Fragments arranged by size:
4545 2113 1663 1143 1057 801 568 516
166 128 121 29
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4391 5408 6881 9137 9710 10954
Size: 197 926 2448 1017 1473 2256 573 1244
Cuts at: 10954 11855 12117 820
Size: 901 262 1553
Fragments arranged by size:
2448 2256 1553 1473 1244 1017 926 901
573 262 197
SnaBI TAC'GTA
Cuts at: 493 10641 493
Size: 10148 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12850
SphI G_CATG'C
Cuts at: 1132 6101 6896 9350 10208 1132
Size: 4969 795 2454 858 3774
Fragments arranged by size:
4969 3774 2454 858 795
SrfI GCCC'GGGC
Cuts at: 4652 7007 4652
Size: 2355 10495
SspI AAT'ATT
Cuts at: 5 52 10774 5
Size: 47 10722 2081
Fragments arranged by size:
10722 2081 47
StuI AGG'CCT
Cuts at: 1517 4232 5117 8457 8921 9768 1517
Size: 2715 885 3340 464 847 4599
Fragments arranged by size:
4599 3340 2715 885 847 464
StyI C'CwwG_G
Cuts at: 513 1065 2004 2964 4656 6273 6780 7358 7632
Size: 552 939 960 1692 1617 507 578 274
Cuts at: 7632 8040 8290 8365 8452 8533 9523 9687 10289
Size: 408 250 75 87 81 990 164 602
Cuts at: 10289 513
Size: 3074
Fragments arranged by size:
3074 1692 1617 990 960 939 602 578
552 507 408 274 250 164 87 81
75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5202 5697 6005 6082 6317 6348 9725
Size: 1255 3622 495 308 77 235 31 3377
Cuts at: 9725 10877 10894 11047 11232 11677 325
Size: 1152 17 153 185 445 1498
Fragments arranged by size:
3622 3377 1498 1255 1152 495 445 308
235 185 153 77 31 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6026 6165 6546
Size: 274 80 33 51 1443 4049 139 381
Cuts at: 6546 6801 7293 9813 11096 12808 96
Size: 255 492 2520 1283 1712 138
Fragments arranged by size:
4049 2520 1712 1443 1283 492 381 274
255 139 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2533 2565 3469 3501 5939 5971 9290 9322 12810
Size: 32 904 32 2438 32 3319 32 3488
Cuts at: 12810 12842 2533
Size: 32 2541
Fragments arranged by size:
3488 3319 2541 2438 904 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2289 4908 1316
Size: 973 2619 9258
Fragments arranged by size:
9258 2619 973
XbaI T'CTAG_A
Cuts at: 10186 10186
Size: 12850
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3032 5797 5940 7522 9113 9730 3032
Size: 2765 143 1582 1591 617 6152
Fragments arranged by size:
6152 2765 1591 1582 617 143
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 12850
XmaI C'CCGG_G
Cuts at: 1870 2036 2065 2186 3849 4650 5166 5294 5862
Size: 166 29 121 1663 801 516 128 568
Cuts at: 5862 7005 8062 10175 1870
Size: 1143 1057 2113 4545
Fragments arranged by size:
4545 2113 1663 1143 1057 801 568 516
166 128 121 29
XmnI GAAnn'nnTTC
Cuts at: 4379 7043 10979 4379
Size: 2664 3936 6250
Fragments arranged by size:
6250 3936 2664
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5950 9720 10658 276
Size: 53 83 186 936 4416 3770 938 2468
Fragments arranged by size:
4416 3770 2468 938 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BsiWI NruI PacI PspXI PsrI SwaI