Restriction map for
pFN21AE9392
(Circular) (Six-base) MAPSORT of: pFN21AE9392.seq Check: 1531 from: 1 to: 8539
pFN21AE9392 8539 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3340 4381 3340
Size: 1041 7498
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5036 6349 278
Size: 53 83 186 936 3500 1313 2468
Fragments arranged by size:
3500 2468 1313 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2586 5882 1937
Size: 649 3296 4594
Fragments arranged by size:
4594 3296 649
Acc65I G'GTAC_C
Cuts at: 5860 5860
Size: 8539
AclI AA'CG_TT
Cuts at: 1563 6666 7039 1563
Size: 5103 373 3063
Fragments arranged by size:
5103 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2373 3206 4797 5171 5345 5444 6602
Size: 492 249 833 1591 374 174 99 1158
Cuts at: 6602 7986 8254 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1591 1384 1158 833 492 374 268
249 174 99
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8342
AflIII A'CryG_T
Cuts at: 1203 7438 8415 1203
Size: 6235 977 1327
Fragments arranged by size:
6235 1327 977
AgeI A'CCGG_T
Cuts at: 7370 7370
Size: 8539
AhdI GACnn_n'nnGTC
Cuts at: 7268 7268
Size: 8539
AleI CACnn'nnGTG
Cuts at: 1166 4433 1166
Size: 3267 5272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4031 5395 1184
Size: 2847 1364 4328
Fragments arranged by size:
4328 2847 1364
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5522 5554 5522
Size: 32 8507
AlwNI CAG_nnn'CTG
Cuts at: 2031 3091 3404 3464 4373 4516 4931 6282 7854
Size: 1060 313 60 909 143 415 1351 1572
Cuts at: 7854 2031
Size: 2716
Fragments arranged by size:
2716 1572 1351 1060 909 415 313 143
60
ApaLI G'TGCA_C
Cuts at: 2073 3241 6596 7752 2073
Size: 1168 3355 1156 2860
Fragments arranged by size:
3355 2860 1168 1156
ApoI r'AATT_y
Cuts at: 1493 2239 2280 2290 2425 2720 2921 3714 4031
Size: 746 41 10 135 295 201 793 317
Cuts at: 4031 4784 5180 5720 5848 6074 6226 8335 1493
Size: 753 396 540 128 226 152 2109 1697
Fragments arranged by size:
2109 1697 793 753 746 540 396 317
295 226 201 152 135 128 41 10
AseI AT'TA_AT
Cuts at: 160 1032 7093 160
Size: 872 6061 1606
Fragments arranged by size:
6061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8539
AvaI C'yCGr_G
Cuts at: 1870 1943 3980 4026 5864 1870
Size: 73 2037 46 1838 4545
Fragments arranged by size:
4545 2037 1838 73 46
AvrII C'CTAG_G
Cuts at: 3284 3944 3284
Size: 660 7879
BamHI G'GATC_C
Cuts at: 5869 6236 5869
Size: 367 8172
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2884 3439 4157 5860 7315
Size: 325 217 627 1097 555 718 1703 1455
Cuts at: 7315 618
Size: 1842
Fragments arranged by size:
1842 1703 1455 1097 718 627 555 325
217
BanII G_rGCy'C
Cuts at: 1391 3258 5304 5858 1391
Size: 1867 2046 554 4072
Fragments arranged by size:
4072 2046 1867 554
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2130 2580 2652 3230 3629 928
Size: 971 231 450 72 578 399 5838
Fragments arranged by size:
5838 971 578 450 399 231 72
BbvCI CC'TCA_GC
Cuts at: 4172 4172
Size: 8539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6730 6764 1903
Size: 34 4793 34 3678
Fragments arranged by size:
4793 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6431 7641 6431
Size: 1210 7329
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7150 136
Size: 107 122 71 631 605 5478 1525
Fragments arranged by size:
5478 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8534 8534
Size: 8539
BlpI GC'TnA_GC
Cuts at: 3191 5956 3191
Size: 2765 5774
Bme1580I G_kGCm'C
Cuts at: 1790 2077 2301 3245 6600 7756 1790
Size: 287 224 944 3355 1156 2573
Fragments arranged by size:
3355 2573 1156 944 287 224
BmgBI CAC'GTC
Cuts at: 1318 5644 1318
Size: 4326 4213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2041 2970 3039
Size: 416 53 83 399 24 624 929 69
Cuts at: 3039 5537 5594 5692 6270 7228 442
Size: 2498 57 98 578 958 1753
Fragments arranged by size:
2498 1753 958 929 624 578 416 399
98 83 69 57 53 24
BmtI G_CTAG'C
Cuts at: 1056 5372 1056
Size: 4316 4223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3400 3490 3400
Size: 90 8449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3349 3441 4143 4538 5045 5434 7199 1239
Size: 2110 92 702 395 507 389 1765 2579
Fragments arranged by size:
2579 2110 1765 702 507 395 389 92
Bpu10I CC'TnA_GC
Cuts at: 3465 4172 3465
Size: 707 7832
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2544 3194 3233 6664 7529 7827 2544
Size: 650 39 3431 865 298 3256
Fragments arranged by size:
3431 3256 865 650 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2748 4076 5077 7202 882
Size: 617 1249 1328 1001 2125 2219
Fragments arranged by size:
2219 2125 1328 1249 1001 617
BsaAI yAC'GTr
Cuts at: 493 1204 2474 6330 493
Size: 711 1270 3856 2702
Fragments arranged by size:
3856 2702 1270 711
BsaBI GATnn'nnATC
Cuts at: 5392 8533 5392
Size: 3141 5398
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5033 6346 6728 7363
Size: 53 83 186 936 3500 1313 382 635
Cuts at: 7363 275
Size: 1451
Fragments arranged by size:
3500 1451 1313 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2707 2887 5079 6972 7370 7644 7791 8202 2707
Size: 180 2192 1893 398 274 147 411 3044
Fragments arranged by size:
3044 2192 1893 411 398 274 180 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2033 2063 5018 5048 5546
Size: 30 165 30 630 30 2955 30 498
Cuts at: 5546 5576 1178
Size: 30 4141
Fragments arranged by size:
4141 2955 630 498 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3573 3659 4535 4638 5294 5390 1186
Size: 2387 86 876 103 656 96 4335
Fragments arranged by size:
4335 2387 876 656 103 96 86
BseYI C'CCAG_C
Cuts at: 2590 3321 3643 5613 5760 7742 8238 2590
Size: 731 322 1970 147 1982 496 2891
Fragments arranged by size:
2891 1982 1970 731 496 322 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3000 1163
Size: 573 1264 6702
Fragments arranged by size:
6702 1264 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5989 6750 7778 1540
Size: 275 186 3988 761 1028 2301
Fragments arranged by size:
3988 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2077 3245 3258 5304 6600 6685 7756 2077
Size: 1168 13 2046 1296 85 1071 2860
Fragments arranged by size:
2860 2046 1296 1168 1071 85 13
BsmI GAATG_Cn'
Cuts at: 3634 5619 6050 6143 3634
Size: 1985 431 93 6030
Fragments arranged by size:
6030 1985 431 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5566 5566
Size: 8539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2301 3245 3258 5304 5858 6600
Size: 399 287 224 944 13 2046 554 742
Cuts at: 6600 6685 7756 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 2046 1071 944 742 554 399 287
224 85 13
BspEI T'CCGG_A
Cuts at: 5079 8202 5079
Size: 3123 5416
BspHI T'CATG_A
Cuts at: 3895 4288 6428 8158 3895
Size: 393 2140 1730 4276
Fragments arranged by size:
4276 2140 1730 393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3340 4381 5894 844
Size: 2496 1041 1513 3489
Fragments arranged by size:
3489 2496 1513 1041
BsrBI CCG'CTC
Cuts at: 1394 1823 2098 6426 1394
Size: 429 275 4328 3507
Fragments arranged by size:
4328 3507 429 275
BsrDI GCAATG_nn'
Cuts at: 65 1248 5242 5322 5527 5583 5665 7034 7208
Size: 1183 3994 80 205 56 82 1369 174
Cuts at: 7208 65
Size: 1396
Fragments arranged by size:
3994 1396 1369 1183 205 174 82 80
56
BsrFI r'CCGG_y
Cuts at: 1700 1810 7183 7370 1700
Size: 110 5373 187 2869
Fragments arranged by size:
5373 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8539
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8539
BssSI C'ACGA_G
Cuts at: 3444 6599 7611 3444
Size: 3155 1012 4372
Fragments arranged by size:
4372 3155 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4373 4847 794
Size: 3579 474 4486
Fragments arranged by size:
4486 3579 474
BstBI TT'CG_AA
Cuts at: 7343 7343
Size: 8539
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8539
BstXI CCAn_nnnn'nTGG
Cuts at: 2516 2516
Size: 8539
BstYI r'GATC_y
Cuts at: 1972 2008 4494 5869 6236 6621 6638 8090 8176
Size: 36 2486 1375 367 385 17 1452 86
Cuts at: 8176 8188 8534 1972
Size: 12 346 1977
Fragments arranged by size:
2486 1977 1452 1375 385 367 346 86
36 17 12
Bsu36I CC'TnA_GG
Cuts at: 4452 5306 4452
Size: 854 7685
BtgI C'CryG_G
Cuts at: 513 1065 2004 2865 3561 3698 4941 5241 5510
Size: 552 939 861 696 137 1243 300 269
Cuts at: 5510 513
Size: 3542
Fragments arranged by size:
3542 1243 939 861 696 552 300 269
137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4717 487
Size: 683 56 3491 4309
Fragments arranged by size:
4309 3491 683 56
BtsI GCAGTG_nn'
Cuts at: 2054 2785 3202 3243 3342 3924 4198 4661 4881
Size: 731 417 41 99 582 274 463 220
Cuts at: 4881 4893 6055 6849 6869 2054
Size: 12 1162 794 20 3724
Fragments arranged by size:
3724 1162 794 731 582 463 417 274
220 99 41 20 12
ClaI AT'CG_AT
Cuts at: 1554 3167 5823 1554
Size: 1613 2656 4270
Fragments arranged by size:
4270 2656 1613
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8504
DraI TTT'AAA
Cuts at: 5843 5843
Size: 8539
DraIII CAC_nnn'GTG
Cuts at: 2275 3240 3446 2275
Size: 965 206 7368
Fragments arranged by size:
7368 965 206
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2582 7546 809
Size: 507 1266 4964 1802
Fragments arranged by size:
4964 1802 1266 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2872 3312 3544
Size: 54 1425 50 275 18 1042 440 232
Cuts at: 3544 4348 4944 5691 5986 6875 8504 8
Size: 804 596 747 295 889 1629 43
Fragments arranged by size:
1629 1425 1042 889 804 747 596 440
295 275 232 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5986 1537
Size: 275 4174 4090
Fragments arranged by size:
4174 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2616 4074 4221 6469 1353
Size: 1263 1458 147 2248 3423
Fragments arranged by size:
3423 2248 1458 1263 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7126 7500 7646 387
Size: 1064 138 5537 374 146 1280
Fragments arranged by size:
5537 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3256 5302 3256
Size: 2046 6493
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2373 3206 3349 3441 4143 4538
Size: 393 492 249 833 143 92 702 395
Cuts at: 4538 4797 5045 5171 5345 5434 5444 6602 7199
Size: 259 248 126 174 89 10 1158 597
Cuts at: 7199 7986 8254 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1158 833 787 702 597 492 395
393 268 259 249 248 174 143 126
92 89 10
EcoNI CCTnn'n_nnAGG
Cuts at: 4520 4520
Size: 8539
EcoO109I rG'GnC_Cy
Cuts at: 3640 4001 4672 5750 3640
Size: 361 671 1078 6429
Fragments arranged by size:
6429 1078 671 361
EcoRI G'AATT_C
Cuts at: 2280 2290 2425 2720 4031 5720 5848 6226 2280
Size: 10 135 295 1311 1689 128 378 4593
Fragments arranged by size:
4593 1689 1311 378 295 135 128 10
EcoRV GAT'ATC
Cuts at: 4809 4809
Size: 8539
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3056 3227 3056
Size: 171 8368
FspI TGC'GCA
Cuts at: 5819 7045 5819
Size: 1226 7313
HaeII r_GCGC'y
Cuts at: 1678 2738 4516 7686 1678
Size: 1060 1778 3170 2531
Fragments arranged by size:
3170 2531 1778 1060
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3413 3445 3559 3591 3752
Size: 32 135 32 1841 32 114 32 161
Cuts at: 3752 3754 3784 3786 3951 3983 4319 4351 5018
Size: 2 30 2 165 32 336 32 667
Cuts at: 5018 5050 5076 5108 5491 5523 5737 5769 7180
Size: 32 26 32 383 32 214 32 1411
Cuts at: 7180 7212 7254 7286 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1841 1411 667 383 336 214 165
161 135 114 42 32 32 32 32
32 32 32 32 32 32 32 32
30 26 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3750 5647 5883 6129 669
Size: 988 281 1812 1897 236 246 3079
Fragments arranged by size:
3079 1897 1812 988 281 246 236
HindIII A'AGCT_T
Cuts at: 748 2329 748
Size: 1581 6958
HpaI GTT'AAC
Cuts at: 6129 6129
Size: 8539
KpnI G_GTAC'C
Cuts at: 5864 5864
Size: 8539
MfeI C'AATT_G
Cuts at: 2916 3780 6138 2916
Size: 864 2358 5317
Fragments arranged by size:
5317 2358 864
MluI A'CGCG_T
Cuts at: 8415 8415
Size: 8539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3372 3619 4089 7653 7837 1305
Size: 2067 247 470 3564 184 2007
Fragments arranged by size:
3564 2067 2007 470 247 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2874 3314 3546 4350 10
Size: 54 1425 343 1042 440 232 804 4199
Fragments arranged by size:
4199 1425 1042 804 440 343 232 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4181 4433 4736 5744 6497 6856 7015
Size: 648 3015 252 303 1008 753 359 159
Cuts at: 7015 518
Size: 2042
Fragments arranged by size:
3015 2042 1008 753 648 359 303 252
159
MspA1I CmG'CkG
Cuts at: 1606 2594 3348 3647 4424 4433 5955 6632 7780
Size: 988 754 299 777 9 1522 677 1148
Cuts at: 7780 8025 1606
Size: 245 2120
Fragments arranged by size:
2120 1522 1148 988 777 754 677 299
245 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 2865 3561 5241 5510 513
Size: 552 939 861 696 1680 269 3542
Fragments arranged by size:
3542 1680 939 861 696 552 269
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8539
NheI G'CTAG_C
Cuts at: 1052 5368 1052
Size: 4316 4223
NotI GC'GGCC_GC
Cuts at: 5986 5986
Size: 8539
NspI r_CATG'y
Cuts at: 1132 1229 5134 5897 7442 1132
Size: 97 3905 763 1545 2229
Fragments arranged by size:
3905 2229 1545 763 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8539
PciI A'CATG_T
Cuts at: 7438 7438
Size: 8539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3486 3601 5836 1108
Size: 2378 115 2235 3811
Fragments arranged by size:
3811 2378 2235 115
PfoI T'CCnGG_A
Cuts at: 4607 4607
Size: 8539
PmeI GTTT'AAAC
Cuts at: 5843 5843
Size: 8539
PmlI CAC'GTG
Cuts at: 2474 2474
Size: 8539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6608 6640 8121 8153 6608
Size: 32 1481 32 6994
Fragments arranged by size:
6994 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3640 4672 3640
Size: 1032 7507
PshAI GACnn'nnGTC
Cuts at: 1613 4424 1613
Size: 2811 5728
PsiI TTA'TAA
Cuts at: 6109 6109
Size: 8539
PstI C_TGCA'G
Cuts at: 830 3337 5891 830
Size: 2507 2554 3478
Fragments arranged by size:
3478 2554 2507
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8539
PvuII CAG'CTG
Cuts at: 2594 3647 4424 4433 2594
Size: 1053 777 9 6700
Fragments arranged by size:
6700 1053 777 9
SacI G_AGCT'C
Cuts at: 3258 5304 3258
Size: 2046 6493
SalI G'TCGA_C
Cuts at: 1936 5881 1936
Size: 3945 4594
SapI GCTCTTCn'nnn_
Cuts at: 4221 4221
Size: 8539
SbfI CC_TGCA'GG
Cuts at: 5891 5891
Size: 8539
ScaI AGT'ACT
Cuts at: 5376 5447 6787 8499 5376
Size: 71 1340 1712 5416
Fragments arranged by size:
5416 1712 1340 71
SfcI C'TryA_G
Cuts at: 826 1045 3333 5887 7022 7703 7894 826
Size: 219 2288 2554 1135 681 191 1471
Fragments arranged by size:
2554 2288 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8539
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8539
SmaI CCC'GGG
Cuts at: 1872 5866 1872
Size: 3994 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2559 3173 3212 6643 7544 7806
Size: 197 926 616 614 39 3431 901 262
Cuts at: 7806 820
Size: 1553
Fragments arranged by size:
3431 1553 926 901 616 614 262 197
39
SnaBI TAC'GTA
Cuts at: 493 6330 493
Size: 5837 2702
SpeI A'CTAG_T
Cuts at: 152 3755 152
Size: 3603 4936
SphI G_CATG'C
Cuts at: 1132 5897 1132
Size: 4765 3774
SspI AAT'ATT
Cuts at: 5 52 3853 5468 6463 5
Size: 47 3801 1615 995 2081
Fragments arranged by size:
3801 2081 1615 995 47
StuI AGG'CCT
Cuts at: 1517 3664 1517
Size: 2147 6392
StyI C'CwwG_G
Cuts at: 513 1065 2004 2865 3284 3309 3561 3944 4143
Size: 552 939 861 419 25 252 383 199
Cuts at: 4143 5241 5343 5510 5800 5978 513
Size: 1098 102 167 290 178 3074
Fragments arranged by size:
3074 1098 939 861 552 419 383 290
252 199 178 167 102 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3574 4300 6566 6583 6736 6921 7366
Size: 1255 1994 726 2266 17 153 185 445
Cuts at: 7366 325
Size: 1498
Fragments arranged by size:
2266 1994 1498 1255 726 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2497 3993 5283
Size: 274 80 33 51 1443 520 1496 1290
Cuts at: 5283 5374 5445 6785 8497 96
Size: 91 71 1340 1712 138
Fragments arranged by size:
1712 1496 1443 1340 1290 520 274 138
91 80 71 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2033 2065 8499 8531 2033
Size: 32 6434 32 2041
Fragments arranged by size:
6434 2041 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3766 1316
Size: 2450 6089
XbaI T'CTAG_A
Cuts at: 4337 5875 4337
Size: 1538 7001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2213 5458 5575 2213
Size: 3245 117 5177
Fragments arranged by size:
5177 3245 117
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8539
XmaI C'CCGG_G
Cuts at: 1870 5864 1870
Size: 3994 4545
XmnI GAAnn'nnTTC
Cuts at: 2691 6668 2691
Size: 3977 4562
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5034 6347 276
Size: 53 83 186 936 3500 1313 2468
Fragments arranged by size:
3500 2468 1313 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BfrBI BsiWI BstZ17I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI