Restriction map for
pFN21AE9289
(Circular) (Six-base) MAPSORT of: pFN21AE9289.seq Check: 6870 from: 1 to: 5617
pFN21AE9289 5617 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2214 2214
Size: 5617
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3427 278
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2960 1937
Size: 1023 4594
Acc65I G'GTAC_C
Cuts at: 2938 2938
Size: 5617
AclI AA'CG_TT
Cuts at: 1563 3744 4117 1563
Size: 2181 373 3063
Fragments arranged by size:
3063 2181 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2740 3680 5064 5332 1632
Size: 1108 940 1384 268 1917
Fragments arranged by size:
1917 1384 1108 940 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5420
AflIII A'CryG_T
Cuts at: 1203 2382 4516 5493 1203
Size: 1179 2134 977 1327
Fragments arranged by size:
2134 1327 1179 977
AgeI A'CCGG_T
Cuts at: 4448 4448
Size: 5617
AhdI GACnn_n'nnGTC
Cuts at: 4346 4346
Size: 5617
AleI CACnn'nnGTG
Cuts at: 1166 2159 1166
Size: 993 4624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2447 1184
Size: 1263 4354
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2303 2335 2303
Size: 32 5585
AlwNI CAG_nnn'CTG
Cuts at: 2072 3360 4932 2072
Size: 1288 1572 2757
Fragments arranged by size:
2757 1572 1288
ApaLI G'TGCA_C
Cuts at: 3674 4830 3674
Size: 1156 4461
ApoI r'AATT_y
Cuts at: 1493 2926 3152 3304 5413 1493
Size: 1433 226 152 2109 1697
Fragments arranged by size:
2109 1697 1433 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4171 160
Size: 872 3139 1606
Fragments arranged by size:
3139 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
AvaI C'yCGr_G
Cuts at: 1870 1943 2942 1870
Size: 73 999 4545
Fragments arranged by size:
4545 999 73
BamHI G'GATC_C
Cuts at: 2947 3314 2947
Size: 367 5250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2938 4393 618
Size: 325 217 627 1151 1455 1842
Fragments arranged by size:
1842 1455 1151 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2094 2112 2196 2604 2936 1391
Size: 703 18 84 408 332 4072
Fragments arranged by size:
4072 703 408 332 84 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4646
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 1903
Size: 34 1871 34 3678
Fragments arranged by size:
3678 1871 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3509 4719 3509
Size: 1210 4407
BclI T'GATC_A
Cuts at: 1548 2178 1548
Size: 630 4987
BfrBI ATG'CAT
Cuts at: 2334 2334
Size: 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4228 136
Size: 107 122 71 631 605 2556 1525
Fragments arranged by size:
2556 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5612 5612
Size: 5617
BlpI GC'TnA_GC
Cuts at: 3034 3034
Size: 5617
Bme1580I G_kGCm'C
Cuts at: 1790 2892 3678 4834 1790
Size: 1102 786 1156 2573
Fragments arranged by size:
2573 1156 1102 786
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5617
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3348 4306 442
Size: 416 53 83 399 24 1931 958 1753
Fragments arranged by size:
1931 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2596 4277 1239
Size: 857 500 1681 2579
Fragments arranged by size:
2579 1681 857 500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2191 2668 3742 4607 4905 2191
Size: 477 1074 865 298 2903
Fragments arranged by size:
2903 1074 865 477 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4280 882
Size: 617 2781 2219
Fragments arranged by size:
2781 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3408 493
Size: 711 2204 2702
Fragments arranged by size:
2702 2204 711
BsaBI GATnn'nnATC
Cuts at: 5611 5611
Size: 5617
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2669 3424 3806 4441
Size: 53 83 186 936 1136 755 382 635
Cuts at: 4441 275
Size: 1451
Fragments arranged by size:
1451 1136 936 755 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4050 4448 4722 4869 5280 4050
Size: 398 274 147 411 4387
Fragments arranged by size:
4387 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2303 2333 1178
Size: 30 165 30 900 30 4462
Fragments arranged by size:
4462 900 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5617
BseYI C'CCAG_C
Cuts at: 2087 4820 5316 2087
Size: 2733 496 2388
Fragments arranged by size:
2733 2388 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5044
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3067 3828 4856 1540
Size: 275 186 1066 761 1028 2301
Fragments arranged by size:
2301 1066 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2112 3678 3763 4834 2112
Size: 1566 85 1071 2895
Fragments arranged by size:
2895 1566 1071 85
BsmI GAATG_Cn'
Cuts at: 2336 2724 2810 3128 3221 2336
Size: 388 86 318 93 4732
Fragments arranged by size:
4732 388 318 93 86
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2112 2196 2604 2892 2936 3678
Size: 399 304 18 84 408 288 44 742
Cuts at: 3678 3763 4834 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 304 288 85
84 44 18
BspEI T'CCGG_A
Cuts at: 5280 5280
Size: 5617
BspHI T'CATG_A
Cuts at: 3506 5236 3506
Size: 1730 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2214 2733 2829 2972 844
Size: 1370 519 96 143 3489
Fragments arranged by size:
3489 1370 519 143 96
BsrBI CCG'CTC
Cuts at: 1394 1823 3504 1394
Size: 429 1681 3507
Fragments arranged by size:
3507 1681 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2706 4112 4286 65
Size: 1183 1458 1406 174 1396
Fragments arranged by size:
1458 1406 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2522 4261 4448 1700
Size: 110 712 1739 187 2869
Fragments arranged by size:
2869 1739 712 187 110
BsrGI T'GTAC_A
Cuts at: 96 2436 96
Size: 2340 3277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5617
BssSI C'ACGA_G
Cuts at: 3677 4689 3677
Size: 1012 4605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5617
BstBI TT'CG_AA
Cuts at: 4421 4421
Size: 5617
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5617
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2287
Size: 5617
BstYI r'GATC_y
Cuts at: 1972 2507 2947 3314 3699 3716 5168 5254 5266
Size: 535 440 367 385 17 1452 86 12
Cuts at: 5266 5612 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 535 440 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2844 513
Size: 552 939 840 3286
Fragments arranged by size:
3286 939 840 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4878
Fragments arranged by size:
4878 683 56
BtsI GCAGTG_nn'
Cuts at: 2461 3133 3927 3947 2461
Size: 672 794 20 4131
Fragments arranged by size:
4131 794 672 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5582
DraI TTT'AAA
Cuts at: 2921 2921
Size: 5617
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4624 809
Size: 507 3308 1802
Fragments arranged by size:
3308 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3064 3953 5582
Size: 54 1425 50 275 18 1234 889 1629
Cuts at: 5582 8
Size: 43
Fragments arranged by size:
1629 1425 1234 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3064 1537
Size: 275 1252 4090
Fragments arranged by size:
4090 1252 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 2591 3547 1353
Size: 776 462 956 3423
Fragments arranged by size:
3423 956 776 462
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4204 4578 4724 387
Size: 1064 138 2615 374 146 1280
Fragments arranged by size:
2615 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2110 2110
Size: 5617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2596 2740 3680 4277 5064 5332
Size: 393 464 500 144 940 597 787 268
Cuts at: 5332 1239
Size: 1524
Fragments arranged by size:
1524 940 787 597 500 464 393 268
144
EcoRI G'AATT_C
Cuts at: 2926 3304 2926
Size: 378 5239
EcoRV GAT'ATC
Cuts at: 2254 2254
Size: 5617
FspI TGC'GCA
Cuts at: 4123 4123
Size: 5617
HaeII r_GCGC'y
Cuts at: 1678 4764 1678
Size: 3086 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2729 2761 4258 4290 4332
Size: 32 135 32 1157 32 1497 32 42
Cuts at: 4332 4364 1373
Size: 32 2626
Fragments arranged by size:
2626 1497 1157 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2961 3207 669
Size: 988 281 1023 246 3079
Fragments arranged by size:
3079 1023 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5617
HpaI GTT'AAC
Cuts at: 3207 3207
Size: 5617
KpnI G_GTAC'C
Cuts at: 2942 2942
Size: 5617
MfeI C'AATT_G
Cuts at: 3216 3216
Size: 5617
MluI A'CGCG_T
Cuts at: 5493 5493
Size: 5617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4731 4915 1305
Size: 3426 184 2007
Fragments arranged by size:
3426 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3795
Fragments arranged by size:
3795 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 3575 3934 4093 518
Size: 648 993 1416 359 159 2042
Fragments arranged by size:
2042 1416 993 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2116 3033 3710 4858 5103 1606
Size: 510 917 677 1148 245 2120
Fragments arranged by size:
2120 1148 917 677 510 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2844 513
Size: 552 939 840 3286
Fragments arranged by size:
3286 939 840 552
NdeI CA'TA_TG
Cuts at: 387 2401 387
Size: 2014 3603
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5617
NotI GC'GGCC_GC
Cuts at: 3064 3064
Size: 5617
NsiI A_TGCA'T
Cuts at: 2336 2336
Size: 5617
NspI r_CATG'y
Cuts at: 1132 1229 2386 2975 4520 1132
Size: 97 1157 589 1545 2229
Fragments arranged by size:
2229 1545 1157 589 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5617
PciI A'CATG_T
Cuts at: 2382 4516 2382
Size: 2134 3483
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2779 1108
Size: 1671 3946
PmeI GTTT'AAAC
Cuts at: 2921 2921
Size: 5617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2303 2335 3686 3718 5199 5231 2303
Size: 32 1351 32 1481 32 2689
Fragments arranged by size:
2689 1481 1351 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5617
PsiI TTA'TAA
Cuts at: 3187 3187
Size: 5617
PstI C_TGCA'G
Cuts at: 830 2426 2809 2826 2969 830
Size: 1596 383 17 143 3478
Fragments arranged by size:
3478 1596 383 143 17
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5617
PvuII CAG'CTG
Cuts at: 2116 2116
Size: 5617
SacI G_AGCT'C
Cuts at: 2112 2112
Size: 5617
SalI G'TCGA_C
Cuts at: 1936 2959 1936
Size: 1023 4594
SbfI CC_TGCA'GG
Cuts at: 2969 2969
Size: 5617
ScaI AGT'ACT
Cuts at: 3865 5577 3865
Size: 1712 3905
SfcI C'TryA_G
Cuts at: 826 1045 2422 2805 2822 2910 2965 4100 4781
Size: 219 1377 383 17 88 55 1135 681
Cuts at: 4781 4972 826
Size: 191 1471
Fragments arranged by size:
1471 1377 1135 681 383 219 191 88
55 17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5617
SmaI CCC'GGG
Cuts at: 1872 2944 1872
Size: 1072 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2170 2647 3721 4622 4884 820
Size: 197 926 227 477 1074 901 262 1553
Fragments arranged by size:
1553 1074 926 901 477 262 227 197
SnaBI TAC'GTA
Cuts at: 493 3408 493
Size: 2915 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5617
SphI G_CATG'C
Cuts at: 1132 2975 1132
Size: 1843 3774
SspI AAT'ATT
Cuts at: 5 52 3541 5
Size: 47 3489 2081
Fragments arranged by size:
3489 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5617
StyI C'CwwG_G
Cuts at: 513 1065 2004 2503 2844 3056 513
Size: 552 939 499 341 212 3074
Fragments arranged by size:
3074 939 552 499 341 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3644 3661 3814 3999 4444 325
Size: 1255 2064 17 153 185 445 1498
Fragments arranged by size:
2064 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2220 2436 3863
Size: 274 80 33 51 1443 243 216 1427
Cuts at: 3863 5575 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1427 274 243 216 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2133 2165 5577 5609 2133
Size: 32 3412 32 2141
Fragments arranged by size:
3412 2141 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5617
XbaI T'CTAG_A
Cuts at: 2953 2953
Size: 5617
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5617
XmaI C'CCGG_G
Cuts at: 1870 2942 1870
Size: 1072 4545
XmnI GAAnn'nnTTC
Cuts at: 2059 3746 2059
Size: 1687 3930
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3425 276
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI