Restriction map for
pFN21AE9282
(Circular) (Six-base) MAPSORT of: pFN21AE9282.seq Check: 341 from: 1 to: 8452
pFN21AE9282 8452 bp
With 182 enzymes: SgfI *
December 8, 2014 13:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4213 4213
Size: 8452
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6262 278
Size: 53 83 186 936 4726 2468
Fragments arranged by size:
4726 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4724 5795 1937
Size: 2787 1071 4594
Fragments arranged by size:
4594 2787 1071
Acc65I G'GTAC_C
Cuts at: 3950 5773 3950
Size: 1823 6629
AclI AA'CG_TT
Cuts at: 1563 3739 4123 6579 6952 1563
Size: 2176 384 2456 373 3063
Fragments arranged by size:
3063 2456 2176 384 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2265 5310 5442 6515 7899 8167 1632
Size: 633 3045 132 1073 1384 268 1917
Fragments arranged by size:
3045 1917 1384 1073 633 268 132
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8255
AflIII A'CryG_T
Cuts at: 1203 7351 8328 1203
Size: 6148 977 1327
Fragments arranged by size:
6148 1327 977
AgeI A'CCGG_T
Cuts at: 7283 7283
Size: 8452
AhdI GACnn_n'nnGTC
Cuts at: 4720 7181 4720
Size: 2461 5991
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8452
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2921 1184
Size: 1737 6715
AlwNI CAG_nnn'CTG
Cuts at: 6195 7767 6195
Size: 1572 6880
ApaLI G'TGCA_C
Cuts at: 4632 6509 7665 4632
Size: 1877 1156 5419
Fragments arranged by size:
5419 1877 1156
ApoI r'AATT_y
Cuts at: 1493 2092 2484 3931 4074 4204 4353 4458 4652
Size: 599 392 1447 143 130 149 105 194
Cuts at: 4652 4801 4867 5595 5761 5987 6139 8248 1493
Size: 149 66 728 166 226 152 2109 1697
Fragments arranged by size:
2109 1697 1447 728 599 392 226 194
166 152 149 149 143 130 105 66
AseI AT'TA_AT
Cuts at: 160 1032 3342 3813 7006 160
Size: 872 2310 471 3193 1606
Fragments arranged by size:
3193 2310 1606 872 471
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8452
AvaI C'yCGr_G
Cuts at: 1870 1943 3069 3210 3258 5072 5777 1870
Size: 73 1126 141 48 1814 705 4545
Fragments arranged by size:
4545 1814 1126 705 141 73 48
BamHI G'GATC_C
Cuts at: 5302 5782 6149 5302
Size: 480 367 7605
Fragments arranged by size:
7605 480 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3950 5773 7228 618
Size: 325 217 627 2163 1823 1455 1842
Fragments arranged by size:
2163 1842 1823 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2793 4270 4528 5073 5771 1391
Size: 1402 1477 258 545 698 4072
Fragments arranged by size:
4072 1477 1402 698 545 258
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 4172 4424 928
Size: 971 102 2171 252 4956
Fragments arranged by size:
4956 2171 971 252 102
BbvCI CC'TCA_GC
Cuts at: 5565 5565
Size: 8452
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3188 3222 3309 3343 6643 6677 1903
Size: 34 1251 34 87 34 3300 34 3678
Fragments arranged by size:
3678 3300 1251 87 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4696 6344 7554 4696
Size: 1648 1210 5594
Fragments arranged by size:
5594 1648 1210
BclI T'GATC_A
Cuts at: 1548 2965 1548
Size: 1417 7035
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7063 136
Size: 107 122 71 631 605 5391 1525
Fragments arranged by size:
5391 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4375 8447 4375
Size: 4072 4380
BlpI GC'TnA_GC
Cuts at: 2164 5869 2164
Size: 3705 4747
Bme1580I G_kGCm'C
Cuts at: 1790 4636 6513 7669 1790
Size: 2846 1877 1156 2573
Fragments arranged by size:
2846 2573 1877 1156
BmgBI CAC'GTC
Cuts at: 1318 2321 1318
Size: 1003 7449
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5681 6183 7141
Size: 416 53 83 399 24 4264 502 958
Cuts at: 7141 442
Size: 1753
Fragments arranged by size:
4264 1753 958 502 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3929 4175 4658 4879 7112 1239
Size: 2690 246 483 221 2233 2579
Fragments arranged by size:
2690 2579 2233 483 246 221
Bpu10I CC'TnA_GC
Cuts at: 5067 5565 5067
Size: 498 7954
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6577 7442 7740 6577
Size: 865 298 7289
Fragments arranged by size:
7289 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7115 882
Size: 617 5616 2219
Fragments arranged by size:
5616 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6243 493
Size: 711 5039 2702
Fragments arranged by size:
5039 2702 711
BsaBI GATnn'nnATC
Cuts at: 3153 8446 3153
Size: 5293 3159
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6259 6641 7276 275
Size: 53 83 186 936 4726 382 635 1451
Fragments arranged by size:
4726 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2793 6885 7283 7557 7704 8115 2793
Size: 4092 398 274 147 411 3130
Fragments arranged by size:
4092 3130 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8227
Fragments arranged by size:
8227 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3551 4206 1186
Size: 2365 655 5432
Fragments arranged by size:
5432 2365 655
BseYI C'CCAG_C
Cuts at: 7655 8151 7655
Size: 496 7956
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7879
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5902 6663 7691 1540
Size: 275 186 3901 761 1028 2301
Fragments arranged by size:
3901 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2793 3211 4039 4270 4528 4636 5073 6513 6598
Size: 418 828 231 258 108 437 1440 85
Cuts at: 6598 7669 2793
Size: 1071 3576
Fragments arranged by size:
3576 1440 1071 828 437 418 258 231
108 85
BsmI GAATG_Cn'
Cuts at: 5963 6056 5963
Size: 93 8359
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2793 3211 4039 4270 4528 4636 5073
Size: 399 1003 418 828 231 258 108 437
Cuts at: 5073 5771 6513 6598 7669 1391
Size: 698 742 85 1071 2174
Fragments arranged by size:
2174 1071 1003 828 742 698 437 418
399 258 231 108 85
BspEI T'CCGG_A
Cuts at: 2793 8115 2793
Size: 5322 3130
BspHI T'CATG_A
Cuts at: 3307 5683 6341 8071 3307
Size: 2376 658 1730 3688
Fragments arranged by size:
3688 2376 1730 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4213 5807 844
Size: 3369 1594 3489
Fragments arranged by size:
3489 3369 1594
BsrBI CCG'CTC
Cuts at: 1394 1823 5281 6339 1394
Size: 429 3458 1058 3507
Fragments arranged by size:
3507 3458 1058 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2524 6947 7121 65
Size: 1183 1276 4423 174 1396
Fragments arranged by size:
4423 1396 1276 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7096 7283 1700
Size: 110 5286 187 2869
Fragments arranged by size:
5286 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8452
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8452
BssSI C'ACGA_G
Cuts at: 6512 7524 6512
Size: 1012 7440
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8452
BstBI TT'CG_AA
Cuts at: 7256 7256
Size: 8452
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8452
BstXI CCAn_nnnn'nTGG
Cuts at: 4490 4490
Size: 8452
BstYI r'GATC_y
Cuts at: 1972 4375 4964 5302 5782 6149 6534 6551 8003
Size: 2403 589 338 480 367 385 17 1452
Cuts at: 8003 8089 8101 8447 1972
Size: 86 12 346 1977
Fragments arranged by size:
2403 1977 1452 589 480 385 367 346
338 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2862 2862
Size: 8452
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7900
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7713
Fragments arranged by size:
7713 683 56
BtsI GCAGTG_nn'
Cuts at: 3453 5968 6762 6782 3453
Size: 2515 794 20 5123
Fragments arranged by size:
5123 2515 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8417
DraI TTT'AAA
Cuts at: 3153 4746 5707 5756 3153
Size: 1593 961 49 5849
Fragments arranged by size:
5849 1593 961 49
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7459 809
Size: 507 6143 1802
Fragments arranged by size:
6143 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2638 4441 5899
Size: 54 1425 50 275 18 808 1803 1458
Cuts at: 5899 6788 8417 8
Size: 889 1629 43
Fragments arranged by size:
1803 1629 1458 1425 889 808 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5899 1537
Size: 275 4087 4090
Fragments arranged by size:
4090 4087 275
EarI CTCTTCn'nnn_
Cuts at: 1353 6382 1353
Size: 5029 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7039 7413 7559 387
Size: 1064 138 5450 374 146 1280
Fragments arranged by size:
5450 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2791 4268 4526 5071 2791
Size: 1477 258 545 6172
Fragments arranged by size:
6172 1477 545 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2265 3929 4175 4658 4879 5310 5442
Size: 393 633 1664 246 483 221 431 132
Cuts at: 5442 6515 7112 7899 8167 1239
Size: 1073 597 787 268 1524
Fragments arranged by size:
1664 1524 1073 787 633 597 483 431
393 268 246 221 132
EcoO109I rG'GnC_Cy
Cuts at: 3965 4473 4486 3965
Size: 508 13 7931
Fragments arranged by size:
7931 508 13
EcoRI G'AATT_C
Cuts at: 2092 2484 4353 5595 5761 6139 2092
Size: 392 1869 1242 166 378 4405
Fragments arranged by size:
4405 1869 1242 392 378 166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 3263 4995 2068
Size: 1195 1732 5525
Fragments arranged by size:
5525 1732 1195
FspI TGC'GCA
Cuts at: 6958 6958
Size: 8452
HaeII r_GCGC'y
Cuts at: 1678 7599 1678
Size: 5921 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3374 3406 4291 4323 4706
Size: 32 135 32 1802 32 885 32 383
Cuts at: 4706 4738 5060 5092 5330 5362 7093 7125 7167
Size: 32 322 32 238 32 1731 32 42
Cuts at: 7167 7199 1373
Size: 32 2626
Fragments arranged by size:
2626 1802 1731 885 383 322 238 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3283 5796 6042 669
Size: 988 281 1345 2513 246 3079
Fragments arranged by size:
3079 2513 1345 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2064 2161 5014 748
Size: 1316 97 2853 4186
Fragments arranged by size:
4186 2853 1316 97
HpaI GTT'AAC
Cuts at: 6042 6042
Size: 8452
KpnI G_GTAC'C
Cuts at: 3954 5777 3954
Size: 1823 6629
MfeI C'AATT_G
Cuts at: 2031 2252 6051 2031
Size: 221 3799 4432
Fragments arranged by size:
4432 3799 221
MluI A'CGCG_T
Cuts at: 8328 8328
Size: 8452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2115 3956 7566 7750 1305
Size: 810 1841 3610 184 2007
Fragments arranged by size:
3610 2007 1841 810 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2640 10
Size: 54 1425 343 808 5822
Fragments arranged by size:
5822 1425 808 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2850 4512 6410 6769 6928 518
Size: 648 1684 1662 1898 359 159 2042
Fragments arranged by size:
2042 1898 1684 1662 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3794 3907 5868 6545 7693 7938 1606
Size: 2188 113 1961 677 1148 245 2120
Fragments arranged by size:
2188 2120 1961 1148 677 245 113
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7900
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8452
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8452
NotI GC'GGCC_GC
Cuts at: 5899 5899
Size: 8452
NspI r_CATG'y
Cuts at: 1132 1229 2274 5737 5810 7355 1132
Size: 97 1045 3463 73 1545 2229
Fragments arranged by size:
3463 2229 1545 1045 97 73
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8452
PciI A'CATG_T
Cuts at: 7351 7351
Size: 8452
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3527 1108
Size: 2419 6033
PfoI T'CCnGG_A
Cuts at: 3674 4833 4923 3674
Size: 1159 90 7203
Fragments arranged by size:
7203 1159 90
PmeI GTTT'AAAC
Cuts at: 5756 5756
Size: 8452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4113 4145 5265 5297 6521 6553 8034 8066 4113
Size: 32 1120 32 1224 32 1481 32 4499
Fragments arranged by size:
4499 1481 1224 1120 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8452
PsiI TTA'TAA
Cuts at: 2061 3648 6022 2061
Size: 1587 2374 4491
Fragments arranged by size:
4491 2374 1587
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3225 3257 3225
Size: 32 8420
PstI C_TGCA'G
Cuts at: 830 3760 4224 5804 830
Size: 2930 464 1580 3478
Fragments arranged by size:
3478 2930 1580 464
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8452
PvuII CAG'CTG
Cuts at: 3794 3907 3794
Size: 113 8339
SacI G_AGCT'C
Cuts at: 2793 4270 4528 5073 2793
Size: 1477 258 545 6172
Fragments arranged by size:
6172 1477 545 258
SalI G'TCGA_C
Cuts at: 1936 5794 1936
Size: 3858 4594
SbfI CC_TGCA'GG
Cuts at: 5804 5804
Size: 8452
ScaI AGT'ACT
Cuts at: 6700 8412 6700
Size: 1712 6740
SfcI C'TryA_G
Cuts at: 826 1045 3331 3756 4220 4379 4841 5800 6935
Size: 219 2286 425 464 159 462 959 1135
Cuts at: 6935 7616 7807 826
Size: 681 191 1471
Fragments arranged by size:
2286 1471 1135 959 681 464 462 425
219 191 159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8452
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8452
SmaI CCC'GGG
Cuts at: 1872 5779 1872
Size: 3907 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6556 7457 7719 820
Size: 197 926 4613 901 262 1553
Fragments arranged by size:
4613 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6243 493
Size: 5750 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8452
SphI G_CATG'C
Cuts at: 1132 2274 5810 1132
Size: 1142 3536 3774
Fragments arranged by size:
3774 3536 1142
SspI AAT'ATT
Cuts at: 5 52 6376 5
Size: 47 6324 2081
Fragments arranged by size:
6324 2081 47
StuI AGG'CCT
Cuts at: 1517 3050 1517
Size: 1533 6919
StyI C'CwwG_G
Cuts at: 513 1065 2308 2989 3969 4069 4489 4644 5891
Size: 552 1243 681 980 100 420 155 1247
Cuts at: 5891 513
Size: 3074
Fragments arranged by size:
3074 1247 1243 980 681 552 420 155
100
SwaI ATTT'AAAT
Cuts at: 3153 3153
Size: 8452
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6479 6496 6649 6834 7279 325
Size: 1255 4899 17 153 185 445 1498
Fragments arranged by size:
4899 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2248 2542 4227
Size: 274 80 33 51 1443 271 294 1685
Cuts at: 4227 4749 6698 8410 96
Size: 522 1949 1712 138
Fragments arranged by size:
1949 1712 1685 1443 522 294 274 271
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3970 4002 4829 4861 8412 8444 3970
Size: 32 827 32 3551 32 3978
Fragments arranged by size:
3978 3551 827 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8452
XbaI T'CTAG_A
Cuts at: 3121 5788 3121
Size: 2667 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8452
XmaI C'CCGG_G
Cuts at: 1870 5777 1870
Size: 3907 4545
XmnI GAAnn'nnTTC
Cuts at: 6581 6581
Size: 8452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6260 276
Size: 53 83 186 936 4726 2468
Fragments arranged by size:
4726 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI XcmI