Restriction map for
pFN21AE9260
(Circular) (Six-base) MAPSORT of: pFN21AE9260.seq Check: 9359 from: 1 to: 9451
pFN21AE9260 9451 bp
With 182 enzymes: SgfI *
February 10, 2017 15:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2611 6597 7261 278
Size: 53 83 186 936 1075 3986 664 2468
Fragments arranged by size:
3986 2468 1075 936 664 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6092 6794 1937
Size: 4155 702 4594
Fragments arranged by size:
4594 4155 702
Acc65I G'GTAC_C
Cuts at: 6772 6772
Size: 9451
AclI AA'CG_TT
Cuts at: 1563 7578 7951 1563
Size: 6015 373 3063
Fragments arranged by size:
6015 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2547 3335 3492 4592 5388 5498 5860 6023
Size: 915 788 157 1100 796 110 362 163
Cuts at: 6023 7514 8898 9166 1632
Size: 1491 1384 268 1917
Fragments arranged by size:
1917 1491 1384 1100 915 796 788 362
268 163 157 110
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9254
AflIII A'CryG_T
Cuts at: 1203 5469 5684 8350 9327 1203
Size: 4266 215 2666 977 1327
Fragments arranged by size:
4266 2666 1327 977 215
AgeI A'CCGG_T
Cuts at: 8282 8282
Size: 9451
AhdI GACnn_n'nnGTC
Cuts at: 6434 6593 8180 6434
Size: 159 1587 7705
Fragments arranged by size:
7705 1587 159
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9451
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9451
AlwNI CAG_nnn'CTG
Cuts at: 2525 2635 6574 7194 8766 2525
Size: 110 3939 620 1572 3210
Fragments arranged by size:
3939 3210 1572 620 110
ApaI G_GGCC'C
Cuts at: 3884 6658 3884
Size: 2774 6677
ApaLI G'TGCA_C
Cuts at: 7508 8664 7508
Size: 1156 8295
ApoI r'AATT_y
Cuts at: 1493 2824 3361 4128 4422 4626 5036 5094 5813
Size: 1331 537 767 294 204 410 58 719
Cuts at: 5813 5865 5948 6760 6986 7138 9247 1493
Size: 52 83 812 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 812 767 719 537 410
294 226 204 152 83 58 52
AseI AT'TA_AT
Cuts at: 160 1032 8005 160
Size: 872 6973 1606
Fragments arranged by size:
6973 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9451
AvaI C'yCGr_G
Cuts at: 1870 1943 3603 5265 6776 1870
Size: 73 1660 1662 1511 4545
Fragments arranged by size:
4545 1662 1660 1511 73
BamHI G'GATC_C
Cuts at: 4435 6781 7148 4435
Size: 2346 367 6738
Fragments arranged by size:
6738 2346 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2070 2227 6394 6684 6772
Size: 325 217 627 283 157 4167 290 88
Cuts at: 6772 8227 618
Size: 1455 1842
Fragments arranged by size:
4167 1842 1455 627 325 290 283 217
157 88
BanII G_rGCy'C
Cuts at: 1391 2217 3818 3884 5622 6231 6658 6770 1391
Size: 826 1601 66 1738 609 427 112 4072
Fragments arranged by size:
4072 1738 1601 826 609 427 112 66
BbeI G_GCGC'C
Cuts at: 6688 6688
Size: 9451
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2885 3631 3774 5376 5488 6592 928
Size: 971 986 746 143 1602 112 1104 3787
Fragments arranged by size:
3787 1602 1104 986 971 746 143 112
BbvCI CC'TCA_GC
Cuts at: 6033 6033
Size: 9451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7642 7676 1903
Size: 34 5705 34 3678
Fragments arranged by size:
5705 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2706 7343 8553 2706
Size: 4637 1210 3604
Fragments arranged by size:
4637 3604 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9451
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4827 6385 8062
Size: 107 122 71 631 605 3155 1558 1677
Cuts at: 8062 136
Size: 1525
Fragments arranged by size:
3155 1677 1558 1525 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2689 2761 4073 9446 2689
Size: 72 1312 5373 2694
Fragments arranged by size:
5373 2694 1312 72
BlpI GC'TnA_GC
Cuts at: 6230 6868 6230
Size: 638 8813
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2230 2519 3399 3884 6658 7512 8668
Size: 283 157 289 880 485 2774 854 1156
Cuts at: 8668 1790
Size: 2573
Fragments arranged by size:
2774 2573 1156 880 854 485 289 283
157
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9451
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7182 8140 442
Size: 416 53 83 399 24 5765 958 1753
Fragments arranged by size:
5765 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9451
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2818 3832 2818
Size: 1014 8437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2523 2582 3866 4313 6315 6366 8111 1239
Size: 1284 59 1284 447 2002 51 1745 2579
Fragments arranged by size:
2579 2002 1745 1284 1284 447 59 51
Bpu10I CC'TnA_GC
Cuts at: 6033 6033
Size: 9451
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2678 2790 3842 3905 6284 7576 8441 8739 2678
Size: 112 1052 63 2379 1292 865 298 3390
Fragments arranged by size:
3390 2379 1292 1052 865 298 112 63
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4281 8114 882
Size: 617 2782 3833 2219
Fragments arranged by size:
3833 2782 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 7242 493
Size: 711 6038 2702
Fragments arranged by size:
6038 2702 711
BsaBI GATnn'nnATC
Cuts at: 2291 2760 9445 2291
Size: 469 6685 2297
Fragments arranged by size:
6685 2297 469
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2608 6594 6685 7258
Size: 53 83 186 936 1075 3986 91 573
Cuts at: 7258 7640 8275 275
Size: 382 635 1451
Fragments arranged by size:
3986 1451 1075 936 635 573 382 186
91 83 53
BsaWI w'CCGG_w
Cuts at: 3786 7884 8282 8556 8703 9114 3786
Size: 4098 398 274 147 411 4123
Fragments arranged by size:
4123 4098 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4878 4908 5714 5744 1178
Size: 30 165 30 3475 30 806 30 4885
Fragments arranged by size:
4885 3475 806 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2125 2756 3824 1186
Size: 939 631 1068 6813
Fragments arranged by size:
6813 1068 939 631
BseYI C'CCAG_C
Cuts at: 3877 6486 8654 9150 3877
Size: 2609 2168 496 4178
Fragments arranged by size:
4178 2609 2168 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3078 1163
Size: 573 1342 7536
Fragments arranged by size:
7536 1342 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6901 7662 8690 1540
Size: 275 186 4900 761 1028 2301
Fragments arranged by size:
4900 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 6231 7512 7597 8668 2217
Size: 4014 1281 85 1071 3000
Fragments arranged by size:
4014 3000 1281 1071 85
BsmI GAATG_Cn'
Cuts at: 3796 6962 7055 3796
Size: 3166 93 6192
Fragments arranged by size:
6192 3166 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2615 3468 2615
Size: 853 8598
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2217 2230 2519 3399 3818 3884
Size: 399 283 144 13 289 880 419 66
Cuts at: 3884 5622 6231 6658 6770 7512 7597 8668 1391
Size: 1738 609 427 112 742 85 1071 2174
Fragments arranged by size:
2174 1738 1071 880 742 609 427 419
399 289 283 144 112 85 66 13
BspEI T'CCGG_A
Cuts at: 3786 9114 3786
Size: 5328 4123
BspHI T'CATG_A
Cuts at: 2872 4191 4371 7340 9070 2872
Size: 1319 180 2969 1730 3253
Fragments arranged by size:
3253 2969 1730 1319 180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4915 5681 6806 844
Size: 4071 766 1125 3489
Fragments arranged by size:
4071 3489 1125 766
BsrBI CCG'CTC
Cuts at: 1394 1823 7338 1394
Size: 429 5515 3507
Fragments arranged by size:
5515 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2700 2751 3739 7946 8120 65
Size: 1183 1452 51 988 4207 174 1396
Fragments arranged by size:
4207 1452 1396 1183 988 174 51
BsrFI r'CCGG_y
Cuts at: 1700 1810 4669 8095 8282 1700
Size: 110 2859 3426 187 2869
Fragments arranged by size:
3426 2869 2859 187 110
BsrGI T'GTAC_A
Cuts at: 96 4211 96
Size: 4115 5336
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9451
BssSI C'ACGA_G
Cuts at: 5526 7511 8523 5526
Size: 1985 1012 6454
Fragments arranged by size:
6454 1985 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2777 3442 794
Size: 1983 665 6803
Fragments arranged by size:
6803 1983 665
BstBI TT'CG_AA
Cuts at: 2440 2896 4132 8255 2440
Size: 456 1236 4123 3636
Fragments arranged by size:
4123 3636 1236 456
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9451
BstXI CCAn_nnnn'nTGG
Cuts at: 4120 6061 4120
Size: 1941 7510
BstYI r'GATC_y
Cuts at: 1972 2137 2531 2556 2689 2761 3300 4073 4435
Size: 165 394 25 133 72 539 773 362
Cuts at: 4435 5928 6781 7148 7533 7550 9002 9088 9100
Size: 1493 853 367 385 17 1452 86 12
Cuts at: 9100 9446 1972
Size: 346 1977
Fragments arranged by size:
1977 1493 1452 853 773 539 394 385
367 362 346 165 133 86 72 25
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 4113 5509 6666 513
Size: 552 939 2109 1396 1157 3298
Fragments arranged by size:
3298 2109 1396 1157 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4820 487
Size: 683 56 3594 5118
Fragments arranged by size:
5118 3594 683 56
BtsI GCAGTG_nn'
Cuts at: 2050 3884 6121 6181 6967 7761 7781 2050
Size: 1834 2237 60 786 794 20 3720
Fragments arranged by size:
3720 2237 1834 794 786 60 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6136 6171 529
Size: 35 5572 35 3809
Fragments arranged by size:
5572 3809 35 35
DraI TTT'AAA
Cuts at: 4864 6755 4864
Size: 1891 7560
DraIII CAC_nnn'GTG
Cuts at: 6341 6341
Size: 9451
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2937 4313 8458 809
Size: 507 1621 1376 4145 1802
Fragments arranged by size:
4145 1802 1621 1376 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2748 4146 6051
Size: 54 1425 50 275 18 918 1398 1905
Cuts at: 6051 6898 7787 9416 8
Size: 847 889 1629 43
Fragments arranged by size:
1905 1629 1425 1398 918 889 847 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6898 1537
Size: 275 5086 4090
Fragments arranged by size:
5086 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2017 2222 2310 2964 3099 3511 3747 3929
Size: 664 205 88 654 135 412 236 182
Cuts at: 3929 5822 5979 7381 1353
Size: 1893 157 1402 3423
Fragments arranged by size:
3423 1893 1402 664 654 412 236 205
182 157 135 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8038 8412 8558 387
Size: 1064 138 6449 374 146 1280
Fragments arranged by size:
6449 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2215 6229 2215
Size: 4014 5437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2523 2547 2582 3335 3492 3866 4313
Size: 393 891 24 35 753 157 374 447
Cuts at: 4313 4592 5388 5498 5860 6023 6315 6366 7514
Size: 279 796 110 362 163 292 51 1148
Cuts at: 7514 8111 8898 9166 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1148 891 796 787 753 597 447
393 374 362 292 279 268 163 157
110 51 35 24
EcoNI CCTnn'n_nnAGG
Cuts at: 6321 6321
Size: 9451
EcoO109I rG'GnC_Cy
Cuts at: 2939 6377 6654 2939
Size: 3438 277 5736
Fragments arranged by size:
5736 3438 277
EcoRI G'AATT_C
Cuts at: 6760 7138 6760
Size: 378 9073
EcoRV GAT'ATC
Cuts at: 3481 3481
Size: 9451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2978 3150 4268 4289 5149 2978
Size: 172 1118 21 860 7280
Fragments arranged by size:
7280 1118 860 172 21
FspI TGC'GCA
Cuts at: 7957 7957
Size: 9451
HaeII r_GCGC'y
Cuts at: 1678 6688 8598 1678
Size: 5010 1910 2531
Fragments arranged by size:
5010 2531 1910
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3098 3130 3465 3497 4154
Size: 32 135 32 1526 32 335 32 657
Cuts at: 4154 4186 4214 4246 4292 4324 4878 4910 5714
Size: 32 28 32 46 32 554 32 804
Cuts at: 5714 5746 6420 6452 6579 6611 8092 8124 8166
Size: 32 674 32 127 32 1481 32 42
Cuts at: 8166 8198 1373
Size: 32 2626
Fragments arranged by size:
2626 1526 1481 804 674 657 554 335
135 127 46 42 32 32 32 32
32 32 32 32 32 32 32 32
32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 2085 6795 7041 669
Size: 988 281 147 4710 246 3079
Fragments arranged by size:
4710 3079 988 281 246 147
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9451
HpaI GTT'AAC
Cuts at: 7041 7041
Size: 9451
KasI G'GCGC_C
Cuts at: 6684 6684
Size: 9451
KpnI G_GTAC'C
Cuts at: 6776 6776
Size: 9451
MfeI C'AATT_G
Cuts at: 5886 7050 5886
Size: 1164 8287
MluI A'CGCG_T
Cuts at: 9327 9327
Size: 9451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2153 2320 2937 3173 4864 6570 8565 8749
Size: 848 167 617 236 1691 1706 1995 184
Cuts at: 8749 1305
Size: 2007
Fragments arranged by size:
2007 1995 1706 1691 848 617 236 184
167
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2750 4148 6053 10
Size: 54 1425 343 918 1398 1905 3408
Fragments arranged by size:
3408 1905 1425 1398 918 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3804 4118 4383 4704 5463 5627 6665
Size: 648 2638 314 265 321 759 164 1038
Cuts at: 6665 7409 7768 7927 518
Size: 744 359 159 2042
Fragments arranged by size:
2638 2042 1038 759 744 648 359 321
314 265 164 159
MspA1I CmG'CkG
Cuts at: 1606 2052 4967 6425 6490 6867 7544 8692 8937
Size: 446 2915 1458 65 377 677 1148 245
Cuts at: 8937 1606
Size: 2120
Fragments arranged by size:
2915 2120 1458 1148 677 446 377 245
65
NarI GG'CG_CC
Cuts at: 6685 6685
Size: 9451
NcoI C'CATG_G
Cuts at: 513 1065 2004 4113 5509 6666 513
Size: 552 939 2109 1396 1157 3298
Fragments arranged by size:
3298 2109 1396 1157 939 552
NdeI CA'TA_TG
Cuts at: 387 4533 6352 387
Size: 4146 1819 3486
Fragments arranged by size:
4146 3486 1819
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9451
NotI GC'GGCC_GC
Cuts at: 6898 6898
Size: 9451
NspI r_CATG'y
Cuts at: 1132 1229 4876 5473 5688 6809 8354 1132
Size: 97 3647 597 215 1121 1545 2229
Fragments arranged by size:
3647 2229 1545 1121 597 215 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9451
PciI A'CATG_T
Cuts at: 5469 5684 8350 5469
Size: 215 2666 6570
Fragments arranged by size:
6570 2666 215
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5515 6310 1108
Size: 4407 795 4249
Fragments arranged by size:
4407 4249 795
PfoI T'CCnGG_A
Cuts at: 2559 3843 6705 2559
Size: 1284 2862 5305
Fragments arranged by size:
5305 2862 1284
PmeI GTTT'AAAC
Cuts at: 6755 6755
Size: 9451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7520 7552 9033 9065 7520
Size: 32 1481 32 7906
Fragments arranged by size:
7906 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2939 2939
Size: 9451
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9451
PsiI TTA'TAA
Cuts at: 2385 2790 3681 3828 7021 2385
Size: 405 891 147 3193 4815
Fragments arranged by size:
4815 3193 891 405 147
PspOMI G'GGCC_C
Cuts at: 3880 6654 3880
Size: 2774 6677
PstI C_TGCA'G
Cuts at: 830 2926 4819 4912 5738 6803 830
Size: 2096 1893 93 826 1065 3478
Fragments arranged by size:
3478 2096 1893 1065 826 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9451
PvuII CAG'CTG
Cuts at: 4967 6425 4967
Size: 1458 7993
SacI G_AGCT'C
Cuts at: 2217 6231 2217
Size: 4014 5437
SalI G'TCGA_C
Cuts at: 1936 6793 1936
Size: 4857 4594
SapI GCTCTTCn'nnn_
Cuts at: 2017 2222 2017
Size: 205 9246
SbfI CC_TGCA'GG
Cuts at: 5738 6803 5738
Size: 1065 8386
ScaI AGT'ACT
Cuts at: 7699 9411 7699
Size: 1712 7739
SexAI A'CCwGG_T
Cuts at: 5431 6337 5431
Size: 906 8545
SfcI C'TryA_G
Cuts at: 826 1045 2133 2685 2922 4402 4815 4855 4908
Size: 219 1088 552 237 1480 413 40 53
Cuts at: 4908 5734 6799 7934 8615 8806 826
Size: 826 1065 1135 681 191 1471
Fragments arranged by size:
1480 1471 1135 1088 1065 826 681 552
413 237 219 191 53 40
SfoI GGC'GCC
Cuts at: 6686 6686
Size: 9451
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9451
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9451
SmaI CCC'GGG
Cuts at: 1872 6778 1872
Size: 4906 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2693 2805 3603 3857 3920 6263
Size: 197 926 750 112 798 254 63 2343
Cuts at: 6263 7555 8456 8718 820
Size: 1292 901 262 1553
Fragments arranged by size:
2343 1553 1292 926 901 798 750 262
254 197 112 63
SnaBI TAC'GTA
Cuts at: 493 7242 493
Size: 6749 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9451
SphI G_CATG'C
Cuts at: 1132 6809 1132
Size: 5677 3774
SspI AAT'ATT
Cuts at: 5 52 2841 7375 5
Size: 47 2789 4534 2081
Fragments arranged by size:
4534 2789 2081 47
StuI AGG'CCT
Cuts at: 1517 4821 6509 1517
Size: 3304 1688 4459
Fragments arranged by size:
4459 3304 1688
StyI C'CwwG_G
Cuts at: 513 1065 2004 2962 3202 4113 5509 6304 6666
Size: 552 939 958 240 911 1396 795 362
Cuts at: 6666 6890 513
Size: 224 3074
Fragments arranged by size:
3074 1396 958 939 911 795 552 362
240 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5613 6222 6472 7478 7495 7648 7833
Size: 1255 4033 609 250 1006 17 153 185
Cuts at: 7833 8278 325
Size: 445 1498
Fragments arranged by size:
4033 1498 1255 1006 609 445 250 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3013 4211 7697
Size: 274 80 33 51 1443 1036 1198 3486
Cuts at: 7697 9409 96
Size: 1712 138
Fragments arranged by size:
3486 1712 1443 1198 1036 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6348 6380 9411 9443 6348
Size: 32 3031 32 6356
Fragments arranged by size:
6356 3031 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4187 5492 1316
Size: 2871 1305 5275
Fragments arranged by size:
5275 2871 1305
XbaI T'CTAG_A
Cuts at: 4070 5041 6787 4070
Size: 971 1746 6734
Fragments arranged by size:
6734 1746 971
XhoI C'TCGA_G
Cuts at: 1943 3603 1943
Size: 1660 7791
XmaI C'CCGG_G
Cuts at: 1870 6776 1870
Size: 4906 4545
XmnI GAAnn'nnTTC
Cuts at: 2625 4128 5649 5813 7580 2625
Size: 1503 1521 164 1767 4496
Fragments arranged by size:
4496 1767 1521 1503 164
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2609 6595 7259 276
Size: 53 83 186 936 1075 3986 664 2468
Fragments arranged by size:
3986 2468 1075 936 664 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstZ17I Bsu36I FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI
SrfI SwaI XcmI