Restriction map for
pFN21AE9257
(Circular) (Six-base) MAPSORT of: pFN21AE9257.seq Check: 1935 from: 1 to: 7282
pFN21AE9257 7282 bp
With 182 enzymes: SgfI *
December 13, 2010 19:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3798 5092 278
Size: 53 83 186 936 2262 1294 2468
Fragments arranged by size:
2468 2262 1294 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4625 1937
Size: 2688 4594
Acc65I G'GTAC_C
Cuts at: 4446 4603 4446
Size: 157 7125
AclI AA'CG_TT
Cuts at: 1563 5409 5782 1563
Size: 3846 373 3063
Fragments arranged by size:
3846 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3425 3548 3719 4041 4500 5345 6729 6997
Size: 1793 123 171 322 459 845 1384 268
Cuts at: 6997 1632
Size: 1917
Fragments arranged by size:
1917 1793 1384 845 459 322 268 171
123
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7085
AflIII A'CryG_T
Cuts at: 1203 3534 6181 7158 1203
Size: 2331 2647 977 1327
Fragments arranged by size:
2647 2331 1327 977
AgeI A'CCGG_T
Cuts at: 6113 6113
Size: 7282
AhdI GACnn_n'nnGTC
Cuts at: 6011 6011
Size: 7282
AleI CACnn'nnGTG
Cuts at: 1166 2531 4208 4373 1166
Size: 1365 1677 165 4075
Fragments arranged by size:
4075 1677 1365 165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3167 1184
Size: 1983 5299
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2223 2255 3500 3532 2223
Size: 32 1245 32 5973
Fragments arranged by size:
5973 1245 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2488 3682 5025 6597 2488
Size: 1194 1343 1572 3173
Fragments arranged by size:
3173 1572 1343 1194
ApaLI G'TGCA_C
Cuts at: 2920 3388 5339 6495 2920
Size: 468 1951 1156 3707
Fragments arranged by size:
3707 1951 1156 468
ApoI r'AATT_y
Cuts at: 1493 3191 4591 4817 4969 7078 1493
Size: 1698 1400 226 152 2109 1697
Fragments arranged by size:
2109 1698 1697 1400 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5836 160
Size: 872 4804 1606
Fragments arranged by size:
4804 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 2203 3436 4103 4607 1870
Size: 73 68 192 1233 667 504 4545
Fragments arranged by size:
4545 1233 667 504 192 73 68
BamHI G'GATC_C
Cuts at: 4612 4979 4612
Size: 367 6915
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3118 3522 4134 4446 4603
Size: 325 217 627 1331 404 612 312 157
Cuts at: 4603 6058 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1331 627 612 404 325 312
217 157
BanII G_rGCy'C
Cuts at: 1391 3513 3951 4601 1391
Size: 2122 438 650 4072
Fragments arranged by size:
4072 2122 650 438
BbeI G_GCGC'C
Cuts at: 3122 3122
Size: 7282
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5473 5507 1903
Size: 34 3536 34 3678
Fragments arranged by size:
3678 3536 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5174 6384 5174
Size: 1210 6072
BclI T'GATC_A
Cuts at: 1548 3096 1548
Size: 1548 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3127 4439 5893
Size: 107 122 71 631 605 1455 1312 1454
Cuts at: 5893 136
Size: 1525
Fragments arranged by size:
1525 1455 1454 1312 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2259 3432 7277 2259
Size: 1173 3845 2264
Fragments arranged by size:
3845 2264 1173
BlpI GC'TnA_GC
Cuts at: 4699 4699
Size: 7282
Bme1580I G_kGCm'C
Cuts at: 1790 2204 2924 3392 5343 6499 1790
Size: 414 720 468 1951 1156 2573
Fragments arranged by size:
2573 1951 1156 720 468 414
BmgBI CAC'GTC
Cuts at: 1318 2494 3226 4303 1318
Size: 1176 732 1077 4297
Fragments arranged by size:
4297 1176 1077 732
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2926 3240 5013
Size: 416 53 83 399 24 1509 314 1773
Cuts at: 5013 5971 442
Size: 958 1753
Fragments arranged by size:
1773 1753 1509 958 416 399 314 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 3047 5942 1239
Size: 1130 678 2895 2579
Fragments arranged by size:
2895 2579 1130 678
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5407 6272 6570 5407
Size: 865 298 6119
Fragments arranged by size:
6119 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4509 5945 882
Size: 617 3010 1436 2219
Fragments arranged by size:
3010 2219 1436 617
BsaAI yAC'GTr
Cuts at: 493 1204 3537 4375 5073 493
Size: 711 2333 838 698 2702
Fragments arranged by size:
2702 2333 838 711 698
BsaBI GATnn'nnATC
Cuts at: 7276 7276
Size: 7282
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3119 3303 3581 3795
Size: 53 83 186 936 1586 184 278 214
Cuts at: 3795 3861 5089 5471 6106 275
Size: 66 1228 382 635 1451
Fragments arranged by size:
1586 1451 1228 936 635 382 278 214
186 184 83 66 53
BsaWI w'CCGG_w
Cuts at: 5715 6113 6387 6534 6945 5715
Size: 398 274 147 411 6052
Fragments arranged by size:
6052 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3500 3530 3603 3633 3780
Size: 30 165 30 2097 30 73 30 147
Cuts at: 3780 3810 1178
Size: 30 4650
Fragments arranged by size:
4650 2097 165 147 73 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2221 3941 4318 1186
Size: 1035 1720 377 4150
Fragments arranged by size:
4150 1720 1035 377
BseYI C'CCAG_C
Cuts at: 2459 3378 3622 3715 4402 6485 6981 2459
Size: 919 244 93 687 2083 496 2760
Fragments arranged by size:
2760 2083 919 687 496 244 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3665 3680 3917 4138 4145 4366 1163
Size: 573 1929 15 237 221 7 221 4079
Fragments arranged by size:
4079 1929 573 237 221 221 15 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 4732 5493 6521 1540
Size: 275 186 1126 1605 761 1028 2301
Fragments arranged by size:
2301 1605 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2924 3392 3951 5343 5428 6499 2924
Size: 468 559 1392 85 1071 3707
Fragments arranged by size:
3707 1392 1071 559 468 85
BsmI GAATG_Cn'
Cuts at: 4793 4886 4793
Size: 93 7189
BsmBI CGTCTCn'nnnn_
Cuts at: 2468 3435 2468
Size: 967 6315
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 2924 3392 3513 3951 4601 5343
Size: 399 414 720 468 121 438 650 742
Cuts at: 5343 5428 6499 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 720 650 468 438 414
399 121 85
BspEI T'CCGG_A
Cuts at: 6945 6945
Size: 7282
BspHI T'CATG_A
Cuts at: 2499 5171 6901 2499
Size: 2672 1730 2880
Fragments arranged by size:
2880 2672 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4027 4637 844
Size: 3183 610 3489
Fragments arranged by size:
3489 3183 610
BsrBI CCG'CTC
Cuts at: 1394 1823 2904 4171 5169 1394
Size: 429 1081 1267 998 3507
Fragments arranged by size:
3507 1267 1081 998 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2857 3360 3834 5777 5951 65
Size: 1183 1609 503 474 1943 174 1396
Fragments arranged by size:
1943 1609 1396 1183 503 474 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 3757 3906 5926 6113 1700
Size: 110 208 1739 149 2020 187 2869
Fragments arranged by size:
2869 2020 1739 208 187 149 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7282
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7282
BssSI C'ACGA_G
Cuts at: 4049 5342 6354 4049
Size: 1293 1012 4977
Fragments arranged by size:
4977 1293 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2039 3643 794
Size: 1245 1604 4433
Fragments arranged by size:
4433 1604 1245
BstBI TT'CG_AA
Cuts at: 2139 6086 2139
Size: 3947 3335
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7282
BstXI CCAn_nnnn'nTGG
Cuts at: 4496 4496
Size: 7282
BstYI r'GATC_y
Cuts at: 1972 2259 3175 3432 4344 4612 4979 5364 5381
Size: 287 916 257 912 268 367 385 17
Cuts at: 5381 6833 6919 6931 7277 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 916 912 385 367 346 287
268 257 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2532 3843 3936 513
Size: 552 1467 1311 93 3859
Fragments arranged by size:
3859 1467 1311 552 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6543
Fragments arranged by size:
6543 683 56
BtsI GCAGTG_nn'
Cuts at: 3644 4798 5592 5612 3644
Size: 1154 794 20 5314
Fragments arranged by size:
5314 1154 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7247
DraI TTT'AAA
Cuts at: 2251 2725 4586 2251
Size: 474 1861 4947
Fragments arranged by size:
4947 1861 474
DraIII CAC_nnn'GTG
Cuts at: 3639 4429 4456 3639
Size: 790 27 6465
Fragments arranged by size:
6465 790 27
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6289 809
Size: 507 4973 1802
Fragments arranged by size:
4973 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3630 3930 4059
Size: 54 1425 50 275 18 1800 300 129
Cuts at: 4059 4530 4729 5618 7247 8
Size: 471 199 889 1629 43
Fragments arranged by size:
1800 1629 1425 889 471 300 275 199
129 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4729 1537
Size: 275 2917 4090
Fragments arranged by size:
4090 2917 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2825 3129 4124 4154 5212 1353
Size: 1472 304 995 30 1058 3423
Fragments arranged by size:
3423 1472 1058 995 304 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3512 5869 6243 6389 387
Size: 1064 138 1923 2357 374 146 1280
Fragments arranged by size:
2357 1923 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3949 3949
Size: 7282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 3047 3425 3548 3719 4041 4500
Size: 393 737 678 378 123 171 322 459
Cuts at: 4500 5345 5942 6729 6997 1239
Size: 845 597 787 268 1524
Fragments arranged by size:
1524 845 787 737 678 597 459 393
378 322 268 171 123
EcoO109I rG'GnC_Cy
Cuts at: 2100 2485 2100
Size: 385 6897
EcoRI G'AATT_C
Cuts at: 4591 4969 4591
Size: 378 6904
EcoRV GAT'ATC
Cuts at: 2185 2185
Size: 7282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4305 4305
Size: 7282
FspI TGC'GCA
Cuts at: 4565 5788 4565
Size: 1223 6059
HaeII r_GCGC'y
Cuts at: 1678 3122 3385 4409 6429 1678
Size: 1444 263 1024 2020 2531
Fragments arranged by size:
2531 2020 1444 1024 263
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3780 3812 4001 4033 5923
Size: 32 135 32 2208 32 189 32 1890
Cuts at: 5923 5955 5997 6029 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2208 1890 189 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2215 4626 4872 669
Size: 988 281 277 2411 246 3079
Fragments arranged by size:
3079 2411 988 281 277 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7282
HpaI GTT'AAC
Cuts at: 2215 4872 2215
Size: 2657 4625
KasI G'GCGC_C
Cuts at: 3118 3118
Size: 7282
KpnI G_GTAC'C
Cuts at: 4450 4607 4450
Size: 157 7125
MfeI C'AATT_G
Cuts at: 4881 4881
Size: 7282
MluI A'CGCG_T
Cuts at: 7158 7158
Size: 7282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2974 3711 6396 6580 1305
Size: 1669 737 2685 184 2007
Fragments arranged by size:
2685 2007 1669 737 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3632 4061 4532 10
Size: 54 1425 343 1800 429 471 2760
Fragments arranged by size:
2760 1800 1425 471 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2312 2531 4121 4208 4373 4473 5240
Size: 648 1146 219 1590 87 165 100 767
Cuts at: 5240 5599 5758 518
Size: 359 159 2042
Fragments arranged by size:
2042 1590 1146 767 648 359 219 165
159 100 87
MspA1I CmG'CkG
Cuts at: 1606 2890 3553 4360 4698 5375 6523 6768 1606
Size: 1284 663 807 338 677 1148 245 2120
Fragments arranged by size:
2120 1284 1148 807 677 663 338 245
NaeI GCC'GGC
Cuts at: 2020 2020
Size: 7282
NarI GG'CG_CC
Cuts at: 3119 3119
Size: 7282
NcoI C'CATG_G
Cuts at: 513 1065 3936 513
Size: 552 2871 3859
Fragments arranged by size:
3859 2871 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7282
NgoMIV G'CCGG_C
Cuts at: 2018 2018
Size: 7282
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7282
NotI GC'GGCC_GC
Cuts at: 4729 4729
Size: 7282
NspI r_CATG'y
Cuts at: 1132 1229 3316 3454 4640 6185 1132
Size: 97 2087 138 1186 1545 2229
Fragments arranged by size:
2229 2087 1545 1186 138 97
PasI CC'CwG_GG
Cuts at: 1348 3745 1348
Size: 2397 4885
PciI A'CATG_T
Cuts at: 6181 6181
Size: 7282
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2403 2488 4456 4528 1108
Size: 1295 85 1968 72 3862
Fragments arranged by size:
3862 1968 1295 85 72
PfoI T'CCnGG_A
Cuts at: 2105 2105
Size: 7282
PmeI GTTT'AAAC
Cuts at: 4586 4586
Size: 7282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 5351 5383 6864 6896 3500
Size: 32 1819 32 1481 32 3886
Fragments arranged by size:
3886 1819 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2100 2100
Size: 7282
PshAI GACnn'nnGTC
Cuts at: 1613 3293 1613
Size: 1680 5602
PsiI TTA'TAA
Cuts at: 4852 4852
Size: 7282
PstI C_TGCA'G
Cuts at: 830 2895 3246 3552 4344 4634 830
Size: 2065 351 306 792 290 3478
Fragments arranged by size:
3478 2065 792 351 306 290
PvuI CG_AT'CG
Cuts at: 2001 3127 2001
Size: 1126 6156
PvuII CAG'CTG
Cuts at: 3553 4360 3553
Size: 807 6475
SacI G_AGCT'C
Cuts at: 3951 3951
Size: 7282
SalI G'TCGA_C
Cuts at: 1936 4624 1936
Size: 2688 4594
SapI GCTCTTCn'nnn_
Cuts at: 4154 4154
Size: 7282
SbfI CC_TGCA'GG
Cuts at: 4634 4634
Size: 7282
ScaI AGT'ACT
Cuts at: 5530 7242 5530
Size: 1712 5570
SexAI A'CCwGG_T
Cuts at: 4425 4452 4425
Size: 27 7255
SfcI C'TryA_G
Cuts at: 826 1045 2891 3242 3548 4340 4630 5765 6446
Size: 219 1846 351 306 792 290 1135 681
Cuts at: 6446 6637 826
Size: 191 1471
Fragments arranged by size:
1846 1471 1135 792 681 351 306 290
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4439 4439
Size: 7282
SfoI GGC'GCC
Cuts at: 3120 3120
Size: 7282
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
SgrAI Cr'CCGG_yG
Cuts at: 1700 3757 1700
Size: 2057 5225
SmaI CCC'GGG
Cuts at: 1872 4105 4609 1872
Size: 2233 504 4545
Fragments arranged by size:
4545 2233 504
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3436 5386 6287 6549 820
Size: 197 926 1493 1950 901 262 1553
Fragments arranged by size:
1950 1553 1493 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5073 493
Size: 4580 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7282
SphI G_CATG'C
Cuts at: 1132 3454 4640 1132
Size: 2322 1186 3774
Fragments arranged by size:
3774 2322 1186
SspI AAT'ATT
Cuts at: 5 52 2653 5206 5
Size: 47 2601 2553 2081
Fragments arranged by size:
2601 2553 2081 47
StuI AGG'CCT
Cuts at: 1517 4073 4288 1517
Size: 2556 215 4511
Fragments arranged by size:
4511 2556 215
StyI C'CwwG_G
Cuts at: 513 1065 2059 3936 4533 4721 513
Size: 552 994 1877 597 188 3074
Fragments arranged by size:
3074 1877 994 597 552 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2395 4241 5309 5326 5479 5664 6109
Size: 1255 815 1846 1068 17 153 185 445
Cuts at: 6109 325
Size: 1498
Fragments arranged by size:
1846 1498 1255 1068 815 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 4572 5528
Size: 274 80 33 51 1443 30 2565 956
Cuts at: 5528 7240 96
Size: 1712 138
Fragments arranged by size:
2565 1712 1443 956 274 138 80 51
33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2498 2530 2827 2859 3004 3036 3603 3635 7242
Size: 32 297 32 145 32 567 32 3607
Cuts at: 7242 7274 2498
Size: 32 2506
Fragments arranged by size:
3607 2506 567 297 145 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2495 3682 4013 1316
Size: 1179 1187 331 4585
Fragments arranged by size:
4585 1187 1179 331
XbaI T'CTAG_A
Cuts at: 4618 4618
Size: 7282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3841 4450 3841
Size: 609 6673
XhoI C'TCGA_G
Cuts at: 1943 3436 1943
Size: 1493 5789
XmaI C'CCGG_G
Cuts at: 1870 4103 4607 1870
Size: 2233 504 4545
Fragments arranged by size:
4545 2233 504
XmnI GAAnn'nnTTC
Cuts at: 3410 5411 3410
Size: 2001 5281
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3796 5090 276
Size: 53 83 186 936 2262 1294 2468
Fragments arranged by size:
2468 2262 1294 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I EcoNI FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SrfI SwaI